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Nicolas Humbert

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Chemicals & Drugs

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Nicolas Humbert; Peter Schürmann; Andrea Zocchi; Jean-Marc Neuhaus; Thomas R Ward
High-yield production and purification of recombinant T7-tag mature streptavidin in glucose-stressed E. coli.
Nicolas Humbert; Thomas R Ward
Functionality screen of streptavidin mutants by non-denaturing SDS-PAGE using biotin-4-fluorescein.
Christophe Letondor; Anca Pordea; Nicolas Humbert; Anita Ivanova; Sylwester Mazurek; Milko Novic; Thomas R Ward
Artificial transfer hydrogenases based on the biotin-(strept)avidin technology: fine tuning the selectivity by saturation mutagenesis of the host protein.

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All Publications

2008: Nicolas Humbert; Peter Schürmann; Andrea Zocchi; Jean-Marc Neuhaus; Thomas R Ward
High-yield production and purification of recombinant T7-tag mature streptavidin in glucose-stressed E. coli.
Methods in molecular biology (Clifton, N.J.) 2008;418():101-10.
2008: Nicolas Humbert; Thomas R Ward
Functionality screen of streptavidin mutants by non-denaturing SDS-PAGE using biotin-4-fluorescein.
Methods in molecular biology (Clifton, N.J.) 2008;418():63-72.
2006: Christophe Letondor; Anca Pordea; Nicolas Humbert; Anita Ivanova; Sylwester Mazurek; Milko Novic; Thomas R Ward
Artificial transfer hydrogenases based on the biotin-(strept)avidin technology: fine tuning the selectivity by saturation mutagenesis of the host protein.
Journal of the American Chemical Society 2006;128(25):8320-8.
2006: Isolde Le Trong; Nicolas Humbert; Thomas R Ward; Ronald E Stenkamp
Crystallographic analysis of a full-length streptavidin with its C-terminal polypeptide bound in the biotin binding site.
Journal of molecular biology 2006;356(3):738-45.
2005: Gérard Klein; Nicolas Humbert; Julieta Gradinaru; Anita Ivanova; François Gilardoni; Untung E Rusbandi; Thomas R Ward
Tailoring the active site of chemzymes by using a chemogenetic-optimization procedure: towards substrate-specific artificial hydrogenases based on the biotin-avidin technology.
Angewandte Chemie (International ed. in English) 2005;44(47):7764-7.
2005: Christophe Letondor; Nicolas Humbert; Thomas R Ward
Artificial metalloenzymes based on biotin-avidin technology for the enantioselective reduction of ketones by transfer hydrogenation.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(13):4683-7.
2005: Nicolas Humbert; Andrea Zocchi; Thomas R Ward
Electrophoretic behavior of streptavidin complexed to a biotinylated probe: a functional screening assay for biotin-binding proteins.
Electrophoresis 2005;26(1):47-52.
2004: Myriem Skander; Nicolas Humbert; Jérôme Collot; Julieta Gradinaru; Gérard Klein; Andreas Loosli; Jérôme Sauser; Andrea Zocchi; François Gilardoni; Thomas R Ward
Artificial metalloenzymes: (strept)avidin as host for enantioselective hydrogenation by achiral biotinylated rhodium-diphosphine complexes.
Journal of the American Chemical Society 2004;126(44):14411-8.
2003: Jérôme Collot; Julieta Gradinaru; Nicolas Humbert; Myriem Skander; Andrea Zocchi; Thomas R Ward
Artificial metalloenzymes for enantioselective catalysis based on biotin-avidin.
Journal of the American Chemical Society 2003;125(30):9030-1.