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Shin-ichi Ikushiro

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Kaori Yasuda; Mariko Endo; Shin-ichi Ikushiro; Masaki Kamakura; Miho Ohta; Toshiyuki Sakaki
UV-dependent production of 25-hydroxyvitamin D2 in the recombinant yeast cells expressing human CYP2R1.
Hongbo Zhang; Adrian Goldman; Shin-ichi Ikushiro; Peter I Mackenzie; Anne-Sisko Patana; Moshe Finel
Human UDP-glucuronosyltransferase expression in insect cells: ratio of active to inactive recombinant proteins and the effects of a C-terminal his-tag on glucuronidation kinetics.
Shinsuke Uchihashi; Miyu Nishikawa; Toshiyuki Sakaki; Shin-ichi Ikushiro
The critical role of amino acid residue at position 117 of mouse UDP-glucuronosyltransfererase 1a6a and 1a6b in resveratrol glucuronidation.

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2013: Kaori Yasuda; Mariko Endo; Shin-ichi Ikushiro; Masaki Kamakura; Miho Ohta; Toshiyuki Sakaki
UV-dependent production of 25-hydroxyvitamin D2 in the recombinant yeast cells expressing human CYP2R1.
Biochemical and biophysical research communications 2013;434(2):311-5.
2012: Hongbo Zhang; Adrian Goldman; Shin-ichi Ikushiro; Peter I Mackenzie; Anne-Sisko Patana; Moshe Finel
Human UDP-glucuronosyltransferase expression in insect cells: ratio of active to inactive recombinant proteins and the effects of a C-terminal his-tag on glucuronidation kinetics.
Drug metabolism and disposition: the biological fate of chemicals 2012;40(10):1935-44.
2012: Shinsuke Uchihashi; Miyu Nishikawa; Toshiyuki Sakaki; Shin-ichi Ikushiro
The critical role of amino acid residue at position 117 of mouse UDP-glucuronosyltransfererase 1a6a and 1a6b in resveratrol glucuronidation.
Journal of biochemistry 2012;152(4):331-40.
2012: Kaori Yasuda; Shuto Wakayama; Keiko Yamamoto; Shin-ichi Ikushiro; Toshimasa Itoh; Masaki Kamakura; Eiji Munetsuna; Miho Ohta; Toshiyuki Sakaki
Comparison of metabolism of sesamin and episesamin by drug-metabolizing enzymes in human liver.
Drug metabolism and disposition: the biological fate of chemicals 2012;40(10):1917-26.
2012: Yoshihisa Kato; Nobuyuki Koga; Chiho Ohta; Masashi Sekimoto; Sekihiro Tamaki; Shizuo Yamada; Tetsuya Endo; Koichi Haraguchi; Shin-ichi Ikushiro; Masakuni Degawa
Comparative study on 2,2',4,5,5'-pentachlorobiphenyl-mediated decrease in serum thyroxine level between C57BL/6 and its transthyretin-deficient mice.
Toxicology and applied pharmacology 2012;263(3):323-9.
2011: Eiji Munetsuna; Sachie Nakabayashi; Miho Ohta; Midori A Arai; Tai C Chen; Shin-ichi Ikushiro; Masaki Kamakura; Rie Kawanami; Atsushi Kittaka; Kaori Yasuda; Toshiyuki Sakaki
Mechanism of the anti-proliferative action of 25-hydroxy-19-nor-vitamin D(3) in human prostate cells.
Journal of molecular endocrinology 2011;47(2):209-18.
2011: Kaori Yasuda; Shin-ichi Ikushiro; Masaki Kamakura; Eiji Munetsuna; Miho Ohta; Toshiyuki Sakaki
Sequential metabolism of sesamin by cytochrome P450 and UDP-glucuronosyltransferase in human liver.
Drug metabolism and disposition: the biological fate of chemicals 2011;39(9):1538-45.
2011: Yoshihisa Kato; Nobuyuki Koga; Chiho Ohta; Mao Onishi; Shizuo Yamada; Tetsuya Endo; Koichi Haraguchi; Shin-ichi Ikushiro; Masakuni Degawa
A possible mechanism for 2,2',4,4',5,5'-hexachlorobiphenyl-mediated decrease in serum thyroxine level in mice.
Toxicology and applied pharmacology 2011;254(1):48-55.
2011: Shinsuke Uchihashi; Hiroyuki Fukumoto; Makoto Onoda; Hiroyoshi Hayakawa; Shin-ichi Ikushiro; Toshiyuki Sakaki
Metabolism of the c-Fos/activator protein-1 inhibitor T-5224 by multiple human UDP-glucuronosyltransferase isoforms.
Drug metabolism and disposition: the biological fate of chemicals 2011;39(5):803-13.
2011: Toshiyuki Sakaki; Hiroshi Sugimoto; Keiko Hayashi; Kaori Yasuda; Eiji Munetsuna; Masaki Kamakura; Shin-ichi Ikushiro; Yoshitsugu Shiro
Bioconversion of vitamin D to its active form by bacterial or mammalian cytochrome P450.
Biochimica et biophysica acta 2011;1814(1):249-56.
2010: Yoshihisa Kato; Hiroshi Suzuki; Koichi Haraguchi; Shin-ichi Ikushiro; Yoshihiko Ito; Shinya Uchida; Shizuo Yamada; Masakuni Degawa
A possible mechanism for the decrease in serum thyroxine level by phenobarbital in rodents.
Toxicology and applied pharmacology 2010;249(3):238-46.
2010: Keiko Hayashi; Kaori Yasuda; Hiroshi Sugimoto; Shin-ichi Ikushiro; Masaki Kamakura; Atsushi Kittaka; Ronald L Horst; Tai C Chen; Miho Ohta; Yoshitsugu Shiro; Toshiyuki Sakaki
Three-step hydroxylation of vitamin D3 by a genetically engineered CYP105A1: enzymes and catalysis.
The FEBS journal 2010;277(19):3999-4009.
2010: Junko Sugatani; Satoshi Sadamitsu; Masatoshi Kurosawa; Shin-ichi Ikushiro; Toshiyuki Sakaki; Akira Ikari; Masao Miwa
Nutritional status affects fluvastatin-induced hepatotoxicity and myopathy in rats.
Drug metabolism and disposition: the biological fate of chemicals 2010;38(10):1655-64.
2010: Noriyuki Kasai; Shin-ichi Ikushiro; Raku Shinkyo; Kaori Yasuda; Shinji Hirosue; Akira Arisawa; Hirofumi Ichinose; Hiroyuki Wariishi; Toshiyuki Sakaki
Metabolism of mono- and dichloro-dibenzo-p-dioxins by Phanerochaete chrysosporium cytochromes P450.
Applied microbiology and biotechnology 2010;86(2):773-80.
2010: Shin-ichi Ikushiro
Takashi Iyanagi: UGT1 gene complex: from Gunn rat to human.
Drug metabolism reviews 2010;42(1):14-22.
2010: Noriyuki Kasai; Shin-ichi Ikushiro; Shinji Hirosue; Akira Arisawa; Hirofumi Ichinose; Yujirou Uchida; Hiroyuki Wariishi; Miho Ohta; Toshiyuki Sakaki
Atypical kinetics of cytochromes P450 catalysing 3'-hydroxylation of flavone from the white-rot fungus Phanerochaete chrysosporium.
Journal of biochemistry 2010;147(1):117-25.
2010: Yoshihisa Kato; Koichi Haraguchi; Makiko Kubota; Yoshiki Seto; Takashi Okura; Shin-ichi Ikushiro; Nobuyuki Koga; Shizuo Yamada; Masakuni Degawa
A possible mechanism for the decrease in serum thyroxine level by a 2,3,7,8-tetrachlorodibenzo-p-dioxin-like polychlorinated biphenyl congener, 3,3',4,4',5-pentachlorobiphenyl in mice.
Drug metabolism and disposition: the biological fate of chemicals 2010;38(1):150-6.
2010: Ryoichi Fujiwara; Miki Nakajima; Shingo Oda; Hiroyuki Yamanaka; Shin-ichi Ikushiro; Toshiyuki Sakaki; Tsuyoshi Yokoi
Interactions between human UDP-glucuronosyltransferase (UGT) 2B7 and UGT1A enzymes.
Journal of pharmaceutical sciences 2010;99(1):442-54.
2009: Yoshihisa Kato; Koichi Haraguchi; Makiko Kubota; Yoshiki Seto; Shin-ichi Ikushiro; Toshiyuki Sakaki; Nobuyuki Koga; Shizuo Yamada; Masakuni Degawa
4-Hydroxy-2,2',3,4',5,5',6-heptachlorobiphenyl-mediated decrease in serum thyroxine level in mice occurs through increase in accumulation of thyroxine in the liver.
Drug metabolism and disposition: the biological fate of chemicals 2009;37(10):2095-102.
2009: Noriyuki Kasai; Shin-ichi Ikushiro; Shinji Hirosue; Akira Arisawa; Hirofumi Ichinose; Hiroyuki Wariishi; Miho Ohta; Toshiyuki Sakaki
Enzymatic properties of cytochrome P450 catalyzing 3'-hydroxylation of naringenin from the white-rot fungus Phanerochaete chrysosporium.
Biochemical and biophysical research communications 2009;387(1):103-8.
2009: Takeshi Izukawa; Miki Nakajima; Ryoichi Fujiwara; Hiroyuki Yamanaka; Tatsuki Fukami; Masataka Takamiya; Yasuhiro Aoki; Shin-ichi Ikushiro; Toshiyuki Sakaki; Tsuyoshi Yokoi
Quantitative analysis of UDP-glucuronosyltransferase (UGT) 1A and UGT2B expression levels in human livers.
Drug metabolism and disposition: the biological fate of chemicals 2009;37(8):1759-68.
2008: Mariko Kishi; Yoshikazu Emi; Masao Sakaguchi; Shin-ichi Ikushiro; Takashi Iyanagi
Ontogenic isoform switching of UDP-glucuronosyltransferase family 1 in rat liver.
Biochemical and biophysical research communications 2008;377(3):815-9.
2008: Katriina Itäaho; Peter I Mackenzie; Shin-ichi Ikushiro; John O Miners; Moshe Finel
The configuration of the 17-hydroxy group variably influences the glucuronidation of beta-estradiol and epiestradiol by human UDP-glucuronosyltransferases.
Drug metabolism and disposition: the biological fate of chemicals 2008;36(11):2307-15.
2008: Luis M Veggi; Luciana Pretto; Elena J Ochoa; Viviana A Catania; Marcelo G Luquita; Diego R Taborda; Enrique J Sánchez Pozzi; Shin-ichi Ikushiro; Michael D Coleman; Marcelo G Roma; Aldo D Mottino
Dapsone induces oxidative stress and impairs antioxidant defenses in rat liver.
Life sciences 2008;83(5-6):155-63.
2008: Hiroshi Sugimoto; Raku Shinkyo; Keiko Hayashi; Sachiyo Yoneda; Masato Yamada; Masaki Kamakura; Shin-ichi Ikushiro; Yoshitsugu Shiro; Toshiyuki Sakaki
Crystal structure of CYP105A1 (P450SU-1) in complex with 1alpha,25-dihydroxyvitamin D3.
Biochemistry 2008;47(13):4017-27.
2008: Makoto Osabe; Junko Sugatani; Tomoaki Fukuyama; Shin-ichi Ikushiro; Akira Ikari; Masao Miwa
Expression of hepatic UDP-glucuronosyltransferase 1A1 and 1A6 correlated with increased expression of the nuclear constitutive androstane receptor and peroxisome proliferator-activated receptor alpha in male rats fed a high-fat and high-sucrose diet.
Drug metabolism and disposition: the biological fate of chemicals 2008;36(2):294-302.
2008: Yoshihisa Kato; Shin-ichi Ikushiro; Yoshikazu Emi; Sekihiro Tamaki; Hiroshi Suzuki; Toshiyuki Sakaki; Shizuo Yamada; Masakuni Degawa
Hepatic UDP-glucuronosyltransferases responsible for glucuronidation of thyroxine in humans.
Drug metabolism and disposition: the biological fate of chemicals 2008;36(1):51-5.
2007: Yoshikazu Emi; Shin-ichi Ikushiro; Yoshihisa Kato
Thyroxine-metabolizing rat uridine diphosphate-glucuronosyltransferase 1A7 is regulated by thyroid hormone receptor.
Endocrinology 2007;148(12):6124-33.
2007: Yoshio Nishimura; Shingo Maeda; Shin-ichi Ikushiro; Peter I Mackenzie; Yuji Ishii; Hideyuki Yamada
Inhibitory effects of adenine nucleotides and related substances on UDP-glucuronosyltransferase: structure-effect relationships and evidence for an allosteric mechanism.
Biochimica et biophysica acta 2007;1770(11):1557-66.
2007: María L Ruiz; Silvina S M Villanueva; Marcelo G Luquita; Shin-ichi Ikushiro; Aldo D Mottino; Viviana A Catania
Beneficial effect of spironolactone administration on ethynylestradiol-induced cholestasis in the rat: involvement of up-regulation of multidrug resistance-associated protein 2.
Drug metabolism and disposition: the biological fate of chemicals 2007;35(11):2060-6.
2007: Yuji Ishii; Megumi Iwanaga; Yoshio Nishimura; Shuso Takeda; Shin-ichi Ikushiro; Kiyoshi Nagata; Yasushi Yamazoe; Peter I Mackenzie; Hideyuki Yamada
Protein-protein interactions between rat hepatic cytochromes P450 (P450s) and UDP-glucuronosyltransferases (UGTs): evidence for the functionally active UGT in P450-UGT complex.
Drug metabolism and pharmacokinetics 2007;22(5):367-76.
2007: Yoshihisa Kato; Shin-ichi Ikushiro; Rie Takiguchi; Koichi Haraguchi; Nobuyuki Koga; Shinya Uchida; Toshiyuki Sakaki; Shizuo Yamada; Jun Kanno; Masakuni Degawa
A novel mechanism for polychlorinated biphenyl-induced decrease in serum thyroxine level in rats.
Drug metabolism and disposition: the biological fate of chemicals 2007;35(10):1949-55.
2007: Naoko Urushino; Sachie Nakabayashi; Midori A Arai; Atsushi Kittaka; Tai C Chen; Keiko Yamamoto; Keiko Hayashi; Shigeaki Kato; Miho Ohta; Masaki Kamakura; Shin-ichi Ikushiro; Toshiyuki Sakaki
Kinetic studies of 25-hydroxy-19-nor-vitamin D3 and 1 alpha,25-dihydroxy-19-nor-vitamin D3 hydroxylation by CYP27B1 and CYP24A1.
Drug metabolism and disposition: the biological fate of chemicals 2007;35(9):1482-8.
2007: Ryoichi Fujiwara; Miki Nakajima; Hiroyuki Yamanaka; Akiko Nakamura; Miki Katoh; Shin-ichi Ikushiro; Toshiyuki Sakaki; Tsuyoshi Yokoi
Effects of coexpression of UGT1A9 on enzymatic activities of human UGT1A isoforms.
Drug metabolism and disposition: the biological fate of chemicals 2007;35(5):747-57.
2007: Natsumi Sawada; Keiko Yamamoto; Sachiko Yamada; Shin-ichi Ikushiro; Masaki Kamakura; Miho Ohta; Kuniyo Inouye; Toshiyuki Sakaki
Role of Gln 85 of human CYP27A1 in 25-hydroxyvitamin D(3)-binding and protein folding.
Biochemical and biophysical research communications 2007;355(1):211-6.
2006: Raku Shinkyo; Masaki Kamakura; Shin-ichi Ikushiro; Kuniyo Inouye; Toshiyuki Sakaki
Biodegradation of dioxins by recombinant Escherichia coli expressing rat CYP1A1 or its mutant.
Applied microbiology and biotechnology 2006;72(3):584-90.
2006: Kazuharu Okamura; Yuji Ishii; Shin-ichi Ikushiro; Peter I Mackenzie; Hideyuki Yamada
Fatty acyl-CoA as an endogenous activator of UDP-glucuronosyltransferases.
Biochemical and biophysical research communications 2006;345(4):1649-56.
2006: Hidenori Yoshioka; Noriyuki Kasai; Shin-ichi Ikushiro; Raku Shinkyo; Masaki Kamakura; Miho Ohta; Kuniyo Inouye; Toshiyuki Sakaki
Enzymatic properties of human CYP2W1 expressed in Escherichia coli.
Biochemical and biophysical research communications 2006;345(1):169-74.
2006: Naoko Urushino; Keiko Yamamoto; Norio Kagawa; Shin-ichi Ikushiro; Masaki Kamakura; Sachiko Yamada; Shigeaki Kato; Kuniyo Inouye; Toshiyuki Sakaki
Interaction between mitochondrial CYP27B1 and adrenodoxin: role of arginine 458 of mouse CYP27B1.
Biochemistry 2006;45(14):4405-12.
2006: Shin-ichi Ikushiro; Yoshikazu Emi; Yoshihisa Kato; Shizuo Yamada; Toshiyuki Sakaki
Monospecific antipeptide antibodies against human hepatic UDP-glucuronosyltransferase 1A subfamily (UGT1A) isoforms.
Drug metabolism and pharmacokinetics 2006;21(1):70-4.
2005: Motoyasu Ikeda; Yuichiro Kida; Shin-ichi Ikushiro; Masao Sakaguchi
Manipulation of membrane protein topology on the endoplasmic reticulum by a specific ligand in living cells.
Journal of biochemistry 2005;138(5):631-7.
2005: Yoshihisa Kato; Hiroshi Suzuki; Shin-ichi Ikushiro; Shizuo Yamada; Masakuni Degawa
Decrease in serum thyroxine level by phenobarbital in rats is not necessarily dependent on increase in hepatic UDP-glucuronosyltransferase.
Drug metabolism and disposition: the biological fate of chemicals 2005;33(11):1608-12.
2005: Peter I Mackenzie; Karl Walter Bock; Brian Burchell; Chantal Guillemette; Shin-ichi Ikushiro; Takashi Iyanagi; John O Miners; Ida S Owens; Daniel W Nebert
Nomenclature update for the mammalian UDP glycosyltransferase (UGT) gene superfamily.
Pharmacogenetics and genomics 2005;15(10):677-85.
2005: Yoshikazu Emi; Koichi Ueda; Aki Ohnishi; Shin-ichi Ikushiro; Takashi Iyanagi
Transcriptional enhancement of UDP-glucuronosyltransferase form 1A2 (UGT1A2) by nuclear factor I-A (NFI-A) in rat hepatocytes.
Journal of biochemistry 2005;138(3):313-25.
2005: Noriko Nishimura; Junzo Yonemoto; Hisao Nishimura; Shin-ichi Ikushiro; Chiharu Tohyama
Disruption of thyroid hormone homeostasis at weaning of Holtzman rats by lactational but not in utero exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin.
Toxicological sciences : an official journal of the Society of Toxicology 2005;85(1):607-14.
2005: Noriyuki Kasai; Toshiyuki Sakaki; Raku Shinkyo; Shin-ichi Ikushiro; Takashi Iyanagi; Miho Ohta; Kuniyo Inouye
Metabolism of 26,26,26,27,27,27-F6-1 alpha,23S,25-trihydroxyvitamin D3 by human UDP-glucuronosyltransferase 1A3.
Drug metabolism and disposition: the biological fate of chemicals 2005;33(1):102-7.
2004: Yoshihisa Kato; Shin-ichi Ikushiro; Koichi Haraguchi; Tomoaki Yamazaki; Yuriko Ito; Hiroshi Suzuki; Ryohei Kimura; Shizuo Yamada; Tohru Inoue; Masakuni Degawa
A possible mechanism for decrease in serum thyroxine level by polychlorinated biphenyls in Wistar and Gunn rats.
Toxicological sciences : an official journal of the Society of Toxicology 2004;81(2):309-15.
2004: Noriyuki Kasai; Toshiyuki Sakaki; Raku Shinkyo; Shin-ichi Ikushiro; Takashi Iyanagi; Maya Kamao; Toshio Okano; Miho Ohta; Kuniyo Inouye
Sequential metabolism of 2,3,7-trichlorodibenzo-p-dioxin (2,3,7-triCDD) by cytochrome P450 and UDP-glucuronosyltransferase in human liver microsomes.
Drug metabolism and disposition: the biological fate of chemicals 2004;32(8):870-5.
2004: Shin-ichi Ikushiro; Masahiro Sahara; Yoshikazu Emi; Yoshiyasu Yabusaki; Takashi Iyanagi
Functional co-expression of xenobiotic metabolizing enzymes, rat cytochrome P450 1A1 and UDP-glucuronosyltransferase 1A6, in yeast microsomes.
Biochimica et biophysica acta 2004;1672(2):86-92.
2003: Viviana A Catania; Marcelo G Luquita; Enrique J Sánchez Pozzi; Shin-ichi Ikushiro; Yoshikazu Emi; Takashi Iyanagi; Aldo D Mottino
Effect of spironolactone on the expression of rat hepatic UDP-glucuronosyltransferase.
Biochemical pharmacology 2003;66(1):171-7.
2002: Shin-ichi Ikushiro; Yoshikazu Emi; Takashi Iyanagi
Activation of glucuronidation through reduction of a disulfide bond in rat UDP-glucuronosyltransferase 1A6.
Biochemistry 2002;41(42):12813-20.
2002: Yoshikazu Emi; Satoshi Omura; Shin-ichi Ikushiro; Takashi Iyanagi
Accelerated degradation of mislocalized UDP-glucuronosyltransferase family 1 (UGT1) proteins in Gunn rat hepatocytes.
Archives of biochemistry and biophysics 2002;405(2):163-9.
2000: Yoshikazu Emi; Aki Ohnishi; T Kajimoto; Shin-ichi Ikushiro; Takashi Iyanagi
A 66-base-pair enhancer module activates the expression of a distinct isoform of UDP-glucuronosyltransferase family 1 (UGT1A2) in primary hepatocytes.
Archives of biochemistry and biophysics 2000;378(2):384-92.
1999: Shin-ichi Ikushiro; Yoshikazu Emi; Shigenobu Kimura; Takashi Iyanagi
Chemical modification of rat hepatic microsomes with N-ethylmaleimide results in inactivation of both UDP-N-acetylglucosamine-dependent stimulation of glucuronidation and UDP-glucuronic acid uptake.
Biochimica et biophysica acta 1999;1428(2-3):388-96.
1999: Shigenobu Kimura; Yoshikazu Emi; Shin-ichi Ikushiro; Takashi Iyanagi
Systematic mutations of highly conserved His49 and carboxyl-terminal of recombinant porcine liver NADH-cytochrome b5 reductase solubilized domain.
Biochimica et biophysica acta 1999;1430(2):290-301.
1998: Takashi Iyanagi; Yoshikazu Emi; Shin-ichi Ikushiro
Biochemical and molecular aspects of genetic disorders of bilirubin metabolism.
Biochimica et biophysica acta 1998;1407(3):173-84.
1998: Takashi Iyanagi; Yoshikazu Emi; Shin-ichi Ikushiro
[Gene organization and genetic defect of bilirubin-glucuronosyl transferase].
Seikagaku. The Journal of Japanese Biochemical Society 1998;70(2):105-9.
1997: Shin-ichi Ikushiro; Yoshikazu Emi; Takashi Iyanagi
Protein-protein interactions between UDP-glucuronosyltransferase isozymes in rat hepatic microsomes.
Biochemistry 1997;36(23):7154-61.
1996: Yoshikazu Emi; Shin-ichi Ikushiro; Takashi Iyanagi
Xenobiotic responsive element-mediated transcriptional activation in the UDP-glucuronosyltransferase family 1 gene complex.
The Journal of biological chemistry 1996;271(7):3952-8.
1995: Shin-ichi Ikushiro; Yoshikazu Emi; Takashi Iyanagi
Identification and analysis of drug-responsive expression of UDP-glucuronosyltransferase family 1 (UGT1) isozyme in rat hepatic microsomes using anti-peptide antibodies.
Archives of biochemistry and biophysics 1995;324(2):267-72.
1995: Yoshikazu Emi; Shin-ichi Ikushiro; Takashi Iyanagi
Drug-responsive and tissue-specific alternative expression of multiple first exons in rat UDP-glucuronosyltransferase family 1 (UGT1) gene complex.
Journal of biochemistry 1995;117(2):392-9.