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Taiji Imoto

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Daisuke Kameoka; Tadashi Ueda; Taiji Imoto
Effect of the conformational stability of the CH2 domain on the aggregation and peptide cleavage of a humanized IgG.
Takatoshi Ohkuri; Satoko Nagatomo; Kenji Oda; Takanori So; Taiji Imoto; Tadashi Ueda
A protein's conformational stability is an immunologically dominant factor: evidence that free-energy barriers for protein unfolding limit the immunogenicity of foreign proteins.
Takashi Goto; Takatoshi Ohkuri; Seijiro Shioi; Yoshito Abe; Taiji Imoto; Tadashi Ueda
Crystal structures of K33 mutant hen lysozymes with enhanced activities.

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All Publications

2011: Daisuke Kameoka; Tadashi Ueda; Taiji Imoto
Effect of the conformational stability of the CH2 domain on the aggregation and peptide cleavage of a humanized IgG.
Applied biochemistry and biotechnology 2011;164(5):642-54.
2010: Takatoshi Ohkuri; Satoko Nagatomo; Kenji Oda; Takanori So; Taiji Imoto; Tadashi Ueda
A protein's conformational stability is an immunologically dominant factor: evidence that free-energy barriers for protein unfolding limit the immunogenicity of foreign proteins.
Journal of immunology (Baltimore, Md. : 1950) 2010;185(7):4199-205.
2008: Takashi Goto; Takatoshi Ohkuri; Seijiro Shioi; Yoshito Abe; Taiji Imoto; Tadashi Ueda
Crystal structures of K33 mutant hen lysozymes with enhanced activities.
Journal of biochemistry 2008;144(5):619-23.
2007: Takatoshi Ohkuri; Chika Takeda; Yuichiro Yoshida; Kenji Izuhara; Taiji Imoto; Tadashi Ueda
Expression of human IL-13 receptor alpha2 extracellular domain in Pichia pastoris.
Protein expression and purification 2007;56(1):48-53.
2007: Takashi Goto; Yoshito Abe; Yoshimitsu Kakuta; Kohei Takeshita; Taiji Imoto; Tadashi Ueda
Crystal structure of Tapes japonica Lysozyme with substrate analogue: structural basis of the catalytic mechanism and manifestation of its chitinase activity accompanied by quaternary structural change.
The Journal of biological chemistry 2007;282(37):27459-67.
2007: Daisuke Kameoka; Etsuro Masuzaki; Tadashi Ueda; Taiji Imoto
Effect of buffer species on the unfolding and the aggregation of humanized IgG.
Journal of biochemistry 2007;142(3):383-91.
2007: Tomonori Mishima; Takatoshi Ohkuri; Taiji Imoto; Tadashi Ueda
Analyses of native disulfide bond formations in the early stage of the folding process in mutant lysozymes where the long-range interactions in the denatured state were modulated.
Bioscience, biotechnology, and biochemistry 2007;71(8):2072-4.
2007: Yuichiro Yoshida; Takatoshi Ohkuri; Chika Takeda; Ryota Kuroki; Kenji Izuhara; Taiji Imoto; Tadashi Ueda
Analysis of internal motions of interleukin-13 variant associated with severe bronchial asthma using (15)N NMR relaxation measurements.
Biochemical and biophysical research communications 2007;358(1):292-7.
2007: Tomonori Mishima; Takatoshi Ohkuri; Akira Monji; Taiji Imoto; Tadashi Ueda
A particular hydrophobic cluster in the residual structure of reduced lysozyme drastically affects the amyloid fibrils formation.
Biochemical and biophysical research communications 2007;356(3):769-72.
2006: Tomonori Mishima; Takatoshi Ohkuri; Akira Monji; Taiji Imoto; Tadashi Ueda
Amyloid formation in denatured single-mutant lysozymes where residual structures are modulated.
Protein science : a publication of the Protein Society 2006;15(10):2448-52.
2006: Ayako Yamada; Yuki Nakamura; Daigo Sugita; Shinya Shirosaki; Takatoshi Ohkuri; Hideo Katsukawa; Kazuaki Nonaka; Taiji Imoto; Yuzo Ninomiya
Induction of salivary kallikreins by the diet containing a sweet-suppressive peptide, gurmarin, in the rat.
Biochemical and biophysical research communications 2006;346(2):386-92.
2006: Yuichiro Yoshida; Masakazu Tanaka; Takatoshi Ohkuri; Yoshitsugu Tanaka; Taiji Imoto; Tadashi Ueda
Analysis of internal motions of RNase T1 complexed with a productive substrate involving 15N NMR relaxation measurements.
Journal of biochemistry 2006;140(1):43-8.
2005: Takatoshi Ohkuri; Taiji Imoto; Tadashi Ueda
Effect of W62G mutation of hen lysozyme on the folding in vivo.
Biochemical and biophysical research communications 2005;338(2):820-4.
2005: Takatoshi Ohkuri; Taiji Imoto; Tadashi Ueda
Characterization of refolded hen lysozyme variant lacking two outside disulfide bonds.
Bioscience, biotechnology, and biochemistry 2005;69(6):1206-8.
2005: Yuichiro Yoshida; Takatoshi Ohkuri; S Kino; Tadashi Ueda; Taiji Imoto
Elucidation of the relationship between enzyme activity and internal motion using a lysozyme stabilized by cavity-filling mutations.
Cellular and molecular life sciences : CMLS 2005;62(9):1047-55.
2005: Takatoshi Ohkuri; Seijiro Shioi; Taiji Imoto; Tadashi Ueda
Effect of the structure of the denatured state of lysozyme on the aggregation reaction at the early stages of folding from the reduced form.
Journal of molecular biology 2005;347(1):159-68.
2005: Noriatsu Shigemura; Keiko Yasumatsu; Ryusuke Yoshida; Noritaka Sako; Hideo Katsukawa; Kiyohito Nakashima; Taiji Imoto; Yuzo Ninomiya
The role of the dpa locus in mice.
Chemical senses 2005;30 Suppl 1():i84-5.
2005: K Ueno; Tadashi Ueda; K Sakai; Y Abe; N Hamasaki; M Okamoto; Taiji Imoto
Evidence for a novel racemization process of an asparaginyl residue in mouse lysozyme under physiological conditions.
Cellular and molecular life sciences : CMLS 2005;62(2):199-205.
2004: Seijiro Shioi; Taiji Imoto; Tadashi Ueda
Analysis of the early stage of the folding process of reduced lysozyme using all lysozyme variants containing a pair of cysteines.
Biochemistry 2004;43(18):5488-93.
2003: Yuichiro Yoshida; Takayuki Obita; Y Kokusho; T Ohmura; Tsutomu Katayama; Tadashi Ueda; Taiji Imoto
Identification of the region in Escherichia coli DnaA protein required for specific recognition of the DnaA box.
Cellular and molecular life sciences : CMLS 2003;60(9):1998-2008.
2003: K Takeshita; Yoshio Hashimoto; Tadashi Ueda; Taiji Imoto
A small chimerically bifunctional monomeric protein: Tapes japonica lysozyme.
Cellular and molecular life sciences : CMLS 2003;60(9):1944-51.
2003: Daisuke Kameoka; Tadashi Ueda; Taiji Imoto
A method for the detection of asparagine deamidation and aspartate isomerization of proteins by MALDI/TOF-mass spectrometry using endoproteinase Asp-N.
Journal of biochemistry 2003;134(1):129-35.
2003: Taiji Imoto
[Enjoying research on lysozymes for 42 years].
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan 2003;123(6):377-86.
2003: Takayuki Obita; Tadashi Ueda; Taiji Imoto
Solution structure and activity of mouse lysozyme M.
Cellular and molecular life sciences : CMLS 2003;60(1):176-84.
2002: Yoshiyuki Tsujihata; Yousuke Mizukami; Tadashi Ueda; Takanori So; Taiji Imoto
B-cell repertoire specific for an unfolded self-determinant of mouse lysozyme escape tolerance and dominantly participate in the autoantibody response.
Immunology 2002;107(4):394-402.
2002: Takayuki Obita; Takafumi Iwura; Masayuki Su'etsugu; Yoichiro Yoshida; Yoshitsugu Tanaka; Tsutomu Katayama; Tadashi Ueda; Taiji Imoto
Determination of the secondary structure in solution of the Escherichia coli DnaA DNA-binding domain.
Biochemical and biophysical research communications 2002;299(1):42-8.
2002: Kaori Kunichika; Yoshio Hashimoto; Taiji Imoto
Robustness of hen lysozyme monitored by random mutations.
Protein engineering 2002;15(10):805-9.
2002: Kiyonari Masumoto; Tadashi Ueda; Makiko Nagata; Yuko Yamada; Yuichiro Yoshida; Yoshio Hashimoto; Taiji Imoto
Effects of stereochemistry of sugars on protein stabilities.
Protein and peptide letters 2002;9(5):435-9.
2002: Taiji Imoto
[Foundation of the bases for protein research and its application to the pharmaceutical science field].
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan 2002;122(8):537-46.
2002: Takatoshi Ohkuri; Tadashi Ueda; Yuichiro Yoshida; Yoshito Abe; Naotaka Hamasaki; Taiji Imoto
A metal binding in the polypeptide chain improves the folding efficiency of a denatured and reduced protein.
Biopolymers 2002;64(2):106-14.
2002: Tadahiro Ohmura; Hiroyuki Motoshima; Tadashi Ueda; Taiji Imoto
Fluctuations in free or substrate-complexed lysozyme and a mutant of it detected on x-ray crystallography and comparison with those detected on NMR.
Journal of biochemistry 2002;131(5):701-4.
2002: Tadashi Ueda; Makiko Nagata; Akira Monji; Ichiro Yoshida; Nobutada Tashiro; Taiji Imoto
Effect of sucrose on formation of the beta-amyloid fibrils and D-aspartic acids in Abeta 1-42.
Biological & pharmaceutical bulletin 2002;25(3):375-8.
2002: Akira Monji; Hideo Utsumi; Tadashi Ueda; Taiji Imoto; Ichiro Yoshida; Sadayuki Hashioka; Ken-ichiro Tashiro; Nobutada Tashiro
Amyloid-beta-protein (A beta) (25-35)-associated free radical generation is strongly influenced by the aggregational state of the peptides.
Life sciences 2002;70(7):833-41.
2001: Takatoshi Ohkuri; Tadashi Ueda; M Tsurumaru; Taiji Imoto
Evidence for an initiation site for hen lysozyme folding from the reduced form using its dissected peptide fragments.
Protein engineering 2001;14(11):829-33.
2001: Takanori So; H Ito; M Hirata; Tadashi Ueda; Taiji Imoto
Contribution of conformational stability of hen lysozyme to induction of type 2 T-helper immune responses.
Immunology 2001;104(3):259-68.
2001: Yoshio Hashimoto; O Munemura; K Masumoto; Tadashi Ueda; Taiji Imoto
Thermostability of doubly glycosylated recombinant lysozyme.
Biological & pharmaceutical bulletin 2001;24(10):1102-7.
2001: Tadashi Ueda; M Nagata; Taiji Imoto
Aggregation and chemical reaction in hen lysozyme caused by heating at pH 6 are depressed by osmolytes, sucrose and trehalose.
Journal of biochemistry 2001;130(4):491-6.
2001: Yoshiyuki Tsujihata; Takanori So; Y Hashimoto; Tadashi Ueda; Taiji Imoto
A single amino acid substitution in a self protein is sufficient to trigger autoantibody response.
Molecular immunology 2001;38(5):375-81.
2001: T Ohmura; Tadashi Ueda; Yoshio Hashimoto; Taiji Imoto
Tolerance of point substitution of methionine for isoleucine in hen egg white lysozyme.
Protein engineering 2001;14(6):421-5.
2001: Akira Monji; Hideo Utsumi; T Ueda; Taiji Imoto; Ichiro Yoshida; Sadayuki Hashioka; K Tashiro ; Nobutada Tashiro
The relationship between the aggregational state of the amyloid-beta peptides and free radical generation by the peptides.
Journal of neurochemistry 2001;77(6):1425-32.
2001: T Ohmura; Tadashi Ueda; K Ootsuka; M Saito; Taiji Imoto
Stabilization of hen egg white lysozyme by a cavity-filling mutation.
Protein science : a publication of the Protein Society 2001;10(2):313-20.
2001: Taiji Imoto
Effective protein folding in simple random search.
Biopolymers 2001;58(1):46-9.
2000: Takayuki Obita; Tadashi Ueda; Yoshitsugu Tanaka; Y Hashimoto; Taiji Imoto
Assignment of 1H and 15N resonances of mouse lysozyme M.
Journal of biomolecular NMR 2000;18(4):361-2.
2000: K Masumoto; Tadashi Ueda; H Motoshima; Taiji Imoto
Relationship between local structure and stability in hen egg white lysozyme mutant with alanine substituted for glycine.
Protein engineering 2000;13(10):691-5.
2000: Yoshiyuki Tsujihata; Takanori So; Y Chijiiwa; Y Hashimoto; M Hirata; Tadashi Ueda; Taiji Imoto
Mutant mouse lysozyme carrying a minimal T cell epitope of hen egg lysozyme evokes high autoantibody response.
Journal of immunology (Baltimore, Md. : 1950) 2000;165(7):3606-11.
2000: S Mine; Tadashi Ueda; Yoshio Hashimoto; Taiji Imoto
Analysis of the internal motion of free and ligand-bound human lysozyme by use of 15N NMR relaxation measurement: a comparison with those of hen lysozyme.
Protein science : a publication of the Protein Society 2000;9(9):1669-84.
2000: Yoshio Hashimoto; T Ikenaga; K Tanigawa; Tadashi Ueda; I Ezak; Taiji Imoto
Expression and characterization of human rheumatoid factor single-chain Fv.
Biological & pharmaceutical bulletin 2000;23(8):941-5.
2000: Tadashi Ueda; K Masumoto; R Ishibashi; Takanori So; Taiji Imoto
Remarkable thermal stability of doubly intramolecularly cross-linked hen lysozyme.
Protein engineering 2000;13(3):193-6.
1999: Yoshio Hashimoto; K Tanigawa; M Nakashima; K Sonoda; Tadashi Ueda; Tomomi Watanabe; Taiji Imoto
Construction of the single-chain Fv from 196-14 antibody toward ovarian cancer-associated antigen CA125.
Biological & pharmaceutical bulletin 1999;22(10):1068-72.
1999: K Kunichika; Y Hashimoto; Taiji Imoto
Construction of a screening system for selecting lysozyme mutants unable to form a stable structure from random mutants.
Journal of biochemistry 1999;126(3):584-90.
1999: Hideo Katsukawa; Taiji Imoto; Yuzo Ninomiya
Induction of salivary gurmarin-binding proteins in rats fed gymnema-containing diets.
Chemical senses 1999;24(4):387-92.
1999: Takanori So; H O Ito; Yoshiyuki Tsujihata; M Hirata; Tadashi Ueda; Taiji Imoto
The molecular weight ratio of monomethoxypolyethylene glycol (mPEG) to protein determines the immunotolerogenicity of mPEG proteins.
Protein engineering 1999;12(8):701-5.
1999: T So; H O Ito; M Hirata; Tadashi Ueda; Taiji Imoto
Extended blood half-life of monomethoxypolyethylene glycol-conjugated hen lysozyme is a key parameter controlling immunological tolerogenicity.
Cellular and molecular life sciences : CMLS 1999;55(8-9):1187-94.
1999: Y Ito; Ryota Kuroki; Y Ogata; Yoshio Hashimoto; K Sugimura; Taiji Imoto
Analysis of a catalytic pathway via a covalent adduct of D52E hen egg white mutant lysozyme by further mutation.
Protein engineering 1999;12(4):327-31.
1999: S Mine; Tadashi Ueda; Yoshio Hashimoto; Yoshitsugu Tanaka; Taiji Imoto
High-level expression of uniformly 15N-labeled hen lysozyme in Pichia pastoris and identification of the site in hen lysozyme where phosphate ion binds using NMR measurements.
FEBS letters 1999;448(1):33-7.
1999: S Mine; S Tate; Tadashi Ueda; M Kainosho; Taiji Imoto
Analysis of the relationship between enzyme activity and its internal motion using nuclear magnetic resonance: 15N relaxation studies of wild-type and mutant lysozyme.
Journal of molecular biology 1999;286(5):1547-65.
1999: Hideaki Fujita; M Saeki; K Yasunaga; Tadashi Ueda; Taiji Imoto; Masaru Himeno
In vitro binding study of adaptor protein complex (AP-1) to lysosomal targeting motif (LI-motif).
Biochemical and biophysical research communications 1999;255(1):54-8.
1999: Y Ito; A Yoshikawa; T Hotani; S Fukuda; K Sugimura; Taiji Imoto
Amino acid sequences of lysozymes newly purified from invertebrates imply wide distribution of a novel class in the lysozyme family.
European journal of biochemistry / FEBS 1999;259(1-2):456-61.
1998: Tadashi Ueda; M Tsurumaru; Taiji Imoto
Kinetic measurement of the interaction between a lysozyme and its immobilized substrate analogue by means of surface plasmon resonance.
Journal of biochemistry 1998;124(4):712-6.
1998: Shunsuke Kawamura; Yoshito Abe; Tadashi Ueda; K Masumoto; Taiji Imoto; Nobuyuki Yamasaki; Makoto Kimura
Investigation of the structural basis for thermostability of DNA-binding protein HU from Bacillus stearothermophilus.
The Journal of biological chemistry 1998;273(32):19982-7.
1998: Yuzo Ninomiya; Taiji Imoto; A Yatabe; S Kawamura; Kiyohito Nakashima; Hideo Katsukawa
Enhanced responses of the chorda tympani nerve to nonsugar sweeteners in the diabetic db/db mouse.
The American journal of physiology 1998;274(5 Pt 2):R1324-30.
1998: H O Ito; Takanori So; M Hirata; Tetsuya Koga; Tadashi Ueda; Taiji Imoto
Tolerogenic activity of polyethylene glycol-conjugated lysozyme distinct from that of the native counterpart.
Immunology 1998;93(2):200-7.
1998: Yoshio Hashimoto; Noriko Koyabu; Taiji Imoto
Effects of signal sequences on the secretion of hen lysozyme by yeast: construction of four secretion cassette vectors.
Protein engineering 1998;11(2):75-7.
1998: Yoshito Abe; Tadashi Ueda; K Kawano; Yoshitaka Tanaka; Taiji Imoto
Detection of a local interaction of hen lysozyme under highly denaturing conditions using chemically 13C-enriched methionine resonance.
Journal of biochemistry 1998;123(2):313-7.
1997: T So; H O Ito; Tetsuya Koga; S Watanabe; Tadashi Ueda; Taiji Imoto
Depression of T-cell epitope generation by stabilizing hen lysozyme.
The Journal of biological chemistry 1997;272(51):32136-40.
1997: Yoshito Abe; Tadashi Ueda; Taiji Imoto
An improved method for preparing lysozyme with chemically 13C-enriched methionine residues using 2-aminothiophenol as a reagent of thiolysis.
Journal of biochemistry 1997;122(6):1153-9.
1997: Tadashi Ueda; Y Maeda; T So; Taiji Imoto
Favourable interaction between heavy and light chains arrests the undesirable oligomerization of heavy chains in the refolding of denatured and reduced immunoglobulin G.
Cellular and molecular life sciences : CMLS 1997;53(11-12):929-34.
1997: S Mine; Tadashi Ueda; Yoshio Hashimoto; Taiji Imoto
Improvement of the refolding yield and solubility of hen egg-white lysozyme by altering the Met residue attached to its N-terminus to Ser.
Protein engineering 1997;10(11):1333-8.
1997: Tadahiro Ohmura; Tadashi Ueda; Hiroyuki Motoshima; T Tamura; Taiji Imoto
Analysis of the stability of mutant lysozymes at position 15 using X-ray crystallography.
Journal of biochemistry 1997;122(3):512-7.
1997: Ryota Kuroki; Y Ito; Y Kato; Taiji Imoto
A covalent enzyme-substrate adduct in a mutant hen egg white lysozyme (D52E).
The Journal of biological chemistry 1997;272(32):19976-81.
1997: Hiroyuki Motoshima; Tadashi Ueda; K Masumoto; Y Hashimoto; Y Chijiiwa; Taiji Imoto
Influence of mutations of the N-cap residue, Gly4, on stability and structure of hen lysozyme.
Journal of biochemistry 1997;122(1):25-31.
1997: Hiroyuki Motoshima; S Mine; K Masumoto; Yoshito Abe; H Iwashita; Y Hashimoto; Y Chijiiwa; Tadashi Ueda; Taiji Imoto
Analysis of the stabilization of hen lysozyme by helix macrodipole and charged side chain interaction.
Journal of biochemistry 1997;121(6):1076-81.
1997: H O Ito; Takanori So; Tadashi Ueda; Taiji Imoto; Tetsuya Koga
Prevention of collagen-induced arthritis (CIA) by treatment with polyethylene glycol-conjugated type II collagen; distinct tolerogenic property of the conjugated collagen from the native one.
Clinical and experimental immunology 1997;108(2):213-9.
1997: Taiji Imoto
Stabilization of protein.
Cellular and molecular life sciences : CMLS 1997;53(3):215-23.
1997: H O Ito; Tadashi Ueda; Yoshio Hashimoto; Taiji Imoto; T Koga
Quaternary structure-dependent idiotope and antigen binding of a monoclonal antibody specific for conformational epitope on type II collagen.
Cellular and molecular life sciences : CMLS 1997;53(1):51-60.
1996: T Ueda; T Ohkuri; Taiji Imoto
Identification of the peptide region that folds native conformation in the early stage of the renaturation of reduced lysozyme.
Biochemical and biophysical research communications 1996;228(1):203-8.
1996: Takanori So; Tadashi Ueda; Yoshito Abe; T Nakamata; Taiji Imoto
Situation of monomethoxypolyethylene glycol covalently attached to lysozyme.
Journal of biochemistry 1996;119(6):1086-93.
1996: Hiroyuki Motoshima; Tadashi Ueda; Taiji Imoto
Analysis of the transition state in the unfolding of hen lysozyme by introduction of Gly-Pro and Pro-Gly sequences at the same site.
Journal of biochemistry 1996;119(6):1019-23.
1996: Y Maeda; Hidenori Yamada; Tadashi Ueda; Taiji Imoto
Effect of additives on the renaturation of reduced lysozyme in the presence of 4 M urea.
Protein engineering 1996;9(5):461-5.
1996: Y Maeda; Tadashi Ueda; Taiji Imoto
Effective renaturation of denatured and reduced immunoglobulin G in vitro without assistance of chaperone.
Protein engineering 1996;9(1):95-100.
1996: Takanori So; H O Ito; Tetsuya Koga; Tadashi Ueda; Taiji Imoto
Reduced immunogenicity of monomethoxypolyethylene glycol-modified lysozyme for activation of T cells.
Immunology letters 1996;49(1-2):91-7.
1996: Tadashi Ueda; H Iwashita; Yoshio Hashimoto; Taiji Imoto
Stabilization of lysozyme by introducing N-glycosylation signal sequence.
Journal of biochemistry 1996;119(1):157-61.
1996: Yoshio Hashimoto; Katsushi Yamada; Hiroyuki Motoshima; T Omura; Hidenori Yamada; T Yasukochi; T Miki; Tadashi Ueda; Taiji Imoto
A mutation study of catalytic residue Asp 52 in hen egg lysozyme.
Journal of biochemistry 1996;119(1):145-50.
1995: Tadashi Ueda; Y Abe; T Ohkuri; K Kawano; Y Terada; Taiji Imoto
Kinetically trapped structure in the renaturation of reduced oxindolealanine 62 lysozyme.
Biochemistry 1995;34(49):16178-85.
1995: Hiroyuki Motoshima; Tadashi Ueda; Yoshio Hashimoto; M Tsutsumi; Taiji Imoto
Correlation between the differences in the free energy change and conformational energy in the folded state of hen lysozymes with Gly-Pro and Pro-Gly sequences introduced to the same site.
Journal of biochemistry 1995;118(6):1138-44.
1995: Yoshito Abe; Tadashi Ueda; H Iwashita; Yoshio Hashimoto; Hiroyuki Motoshima; Y Tanaka; Taiji Imoto
Effect of salt concentration on the pKa of acidic residues in lysozyme.
Journal of biochemistry 1995;118(5):946-52.
1995: H Tomizawa; Hidenori Yamada; Yoshio Hashimoto; Taiji Imoto
Stabilization of lysozyme against irreversible inactivation by alterations of the Asp-Gly sequences.
Protein engineering 1995;8(10):1023-8.
1995: Y Ito; M Nakamura; T Hotani; Taiji Imoto
Insect lysozyme from house fly (Musca domestica) larvae: possible digestive function based on sequence and enzymatic properties.
Journal of biochemistry 1995;118(3):546-51.
1995: H Tomizawa; Hidenori Yamada; K Wada; Taiji Imoto
Stabilization of lysozyme against irreversible inactivation by suppression of chemical reactions.
Journal of biochemistry 1995;117(3):635-40.
1995: Y Maeda; H Koga; Hidenori Yamada; Tadashi Ueda; Taiji Imoto
Effective renaturation of reduced lysozyme by gentle removal of urea.
Protein engineering 1995;8(2):201-5.
1995: H Tomizawa; Hidenori Yamada; K Tanigawa; Taiji Imoto
Effects of additives on irreversible inactivation of lysozyme at neutral pH and 100 degrees C.
Journal of biochemistry 1995;117(2):369-73.
1994: Y Ito; M Hirashima; Hidenori Yamada; Taiji Imoto
Colonic lysozymes of rabbit (Japanese white): recent divergence and functional conversion.
Journal of biochemistry 1994;116(6):1346-53.
1994: H Tomizawa; Hidenori Yamada; Taiji Imoto
The mechanism of irreversible inactivation of lysozyme at pH 4 and 100 degrees C.
Biochemistry 1994;33(44):13032-7.
1994: Y Maeda; Tadashi Ueda; Hidenori Yamada; Taiji Imoto
The role of net charge on the renaturation of reduced lysozyme by the sulfhydryl-disulfide interchange reaction.
Protein engineering 1994;7(10):1249-54.
1994: H Tomizawa; Hidenori Yamada; Tadashi Ueda; Taiji Imoto
Isolation and characterization of 101-succinimide lysozyme that possesses the cyclic imide at Asp101-Gly102.
Biochemistry 1994;33(29):8770-4.
1994: Taiji Imoto; Tadashi Ueda; T Tamura; Y Isakari; Y Abe; M Inoue; T Miki; K Kawano; Hidenori Yamada
Lysozyme requires fluctuation of the active site for the manifestation of activity.
Protein engineering 1994;7(6):743-8.
1994: Tadashi Ueda; A Nakashima; Y Hashimoto; T Miki; Hidenori Yamada; Taiji Imoto
Formation of alpha-helix 88-98 is essential in the establishment of higher-order structure from reduced lysozyme.
Journal of molecular biology 1994;235(4):1312-7.
1994: Yoshito Abe; Tadashi Ueda; Taiji Imoto
Reduction of disulfide bonds in proteins by 2-aminothiophenol under weakly acidic conditions.
Journal of biochemistry 1994;115(1):52-7.
1993: Y Ito; Hidenori Yamada; M Nakamura; A Yoshikawa; T Ueda; Taiji Imoto
The primary structures and properties of non-stomach lysozymes of sheep and cow, and implication for functional divergence of lysozyme.
European journal of biochemistry / FEBS 1993;213(2):649-58.
1993: M Kuribayashi; Hidenori Yamada; T Ohmori; M Yanai; Taiji Imoto
Endopeptidase activity of cathepsin C, dipeptidyl aminopeptidase I, from bovine spleen.
Journal of biochemistry 1993;113(4):441-9.
1993: Tadashi Ueda; T Tamura; Y Maeda; Yoshio Hashimoto; T Miki; Hidenori Yamada; Taiji Imoto
Stabilization of lysozyme by the introduction of Gly-Pro sequence.
Protein engineering 1993;6(2):183-7.
1993: Tadashi Ueda; Yoshito Abe; Kenji Akasaki; Y Yamaguchi; H Tsuji; K Kawano; Hidenori Yamada; Taiji Imoto
Detection of subtle differences in the surface structure of lysozymes by use of an immobilized Fab fragment.
Journal of biochemistry 1993;113(2):174-9.
1992: Mayumi Inoue; Hidenori Yamada; T Yasukochi; T Miki; T Horiuchi; Taiji Imoto
Left-sided substrate binding of lysozyme: evidence for the involvement of asparagine-46 in the initial binding of substrate to chicken lysozyme.
Biochemistry 1992;31(42):10322-30.
1992: Mayumi Inoue; Hidenori Yamada; Yoshio Hashimoto; T Yasukochi; K Hamaguchi; T Miki; T Horiuchi; Taiji Imoto
Stabilization of a protein by removal of unfavorable abnormal pKa: substitution of undissociable residue for glutamic acid-35 in chicken lysozyme.
Biochemistry 1992;31(37):8816-21.
1992: Mayumi Inoue; Hidenori Yamada; T Yasukochi; R Kuroki; T Miki; T Horiuchi; Taiji Imoto
Multiple role of hydrophobicity of tryptophan-108 in chicken lysozyme: structural stability, saccharide binding ability, and abnormal pKa of glutamic acid-35.
Biochemistry 1992;31(24):5545-53.
1992: Y Ito; Hidenori Yamada; Taiji Imoto
Colorimetric assay for lysozyme using Micrococcus luteus labeled with a blue dye, Remazol brilliant blue R, as a substrate.
Chemical & pharmaceutical bulletin 1992;40(6):1523-6.
1992: H Koga; N Mori; Hidenori Yamada; Y Nishimura; K Tokuda; K Kato; Taiji Imoto
Endo- and aminopeptidase activities of rat cathepsin H.
Chemical & pharmaceutical bulletin 1992;40(4):965-70.
1991: H Koga; N Mori; Hidenori Yamada; Y Nishimura; K Tokuda; K Kato; Taiji Imoto
Rat cathepsin H-catalyzed transacylation: comparisons of the mechanism and the specificity with papain-superfamily proteases.
Journal of biochemistry 1991;110(6):939-44.
1991: Masaru Himeno; K Nakamura; Y Tanaka; Hidenori Yamada; Taiji Imoto; K Kato
Mechanisms of a conversion from membrane associated lysosomal acid phosphatase to content forms.
Biochemical and biophysical research communications 1991;180(3):1483-9.
1991: Tadashi Ueda; Hidenori Yamada; N Sakamoto; Yoshito Abe; K Kawano; Y Terada; Taiji Imoto
Preparation and properties of a lysozyme derivative in which two domains are cross-linked intramolecularly between Trp62 and Asp101.
Journal of biochemistry 1991;110(5):719-25.
1991: H Koga; Hidenori Yamada; Y Nishimura; K Kato; Taiji Imoto
Multiple proteolytic action of rat liver cathepsin B: specificities and pH-dependences of the endo- and exopeptidase activities.
Journal of biochemistry 1991;110(2):179-88.
1991: Tadashi Ueda; Y Isakari; H Aoki; T Yasukochi; S Masutomo; K Kawano; Y Terada; Hidenori Yamada; Taiji Imoto
1H-NMR study of the intramolecular interaction of a substrate analogue covalently attached to aspartic acid-101 in lysozyme.
Journal of biochemistry 1991;109(5):690-8.
1990: H Koga; Hidenori Yamada; Y Nishimura; K Kato; Taiji Imoto
Comparative study on specificities of rat cathepsin L and papain: amino acid differences at substrate-binding sites are involved in their specificities.
Journal of biochemistry 1990;108(6):976-82.
1990: Tadashi Ueda; Hidenori Yamada; H Aoki; Taiji Imoto
Effect of chemical modifications of tryptophan residues on the folding of reduced hen egg-white lysozyme.
Journal of biochemistry 1990;108(5):886-92.
1990: H Yamada; T Yamashita; H Domoto; Taiji Imoto
Reaction of hen egg-white lysozyme with tetranitromethane: a new side reaction, oxidative bond cleavage at glycine 104, and sequential nitration of three tyrosine residues.
Journal of biochemistry 1990;108(3):432-40.
1990: Y Ito; H Yamada; S Nakamura; Taiji Imoto
Purification, amino acid sequence, and some properties of rabbit kidney lysozyme.
Journal of biochemistry 1990;107(2):236-41.
1987: Taiji Imoto; H Yamada; T Yasukochi; E Yamada; Y Ito; Tadashi Ueda; H Nagatani; T Miki; T Horiuchi
Point mutation of alanine (31) to valine prohibits the folding of reduced lysozyme by sulfhydryl-disulfide interchange.
Protein engineering 1987;1(4):333-8.
1987: T Miki; T Yasukochi; H Nagatani; M Furuno; T Orita; H Yamada; Taiji Imoto; T Horiuchi
Construction of a plasmid vector for the regulatable high level expression of eukaryotic genes in Escherichia coli: an application to overproduction of chicken lysozyme.
Protein engineering 1987;1(4):327-32.
1987: Tadashi Ueda; H Yamada; Taiji Imoto
Highly controlled carbodiimide reaction for the modification of lysozyme. Modification of Leu129 or Asp119.
Protein engineering 1987;1(3):189-93.
1985: H Yamada; Tadashi Ueda; R Kuroki; T Fukumura; T Yasukochi; T Hirabayashi; K Fujita; Taiji Imoto
Isolation and characterization of 101-beta-lysozyme that possesses the beta-aspartyl sequence at aspartic acid-101.
Biochemistry 1985;24(27):7953-9.