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Hubertus Irth

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Danielle S Counotte; Natalia A Goriounova; Cecilia Gotti; Hubertus Irth; Huibert D Mansvelder; Milena Moretti; Anton N M Schoffelmeer; August B Smit; Marek T Smoluch; Francesco Clementi; Sabine Spijker
Adolescent nicotine exposure transiently increases high-affinity nicotinic receptors and modulates inhibitory synaptic transmission in rat medial prefrontal cortex.
David Falck; Martin Giera; Maarten Honing; Hubertus Irth; Jon S B de Vlieger; Wilfried M A Niessen; Jeroen Kool
On-line electrochemistry-bioaffinity screening with parallel HR-LC-MS for the generation and characterization of modified p38α kinase inhibitors.
Jeroen Kool; Rob Leurs; Henk Lingeman; Iwan J P De Esch; Gerdien de Kloe; Ewald Edink; Ferry Heus; Hubertus Irth; August B Smit; Wilfried M A Niessen
High-resolution bioactivity profiling of mixtures toward the acetylcholine binding protein using a nanofractionation spotter technology.

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2012: Danielle S Counotte; Natalia A Goriounova; Cecilia Gotti; Hubertus Irth; Huibert D Mansvelder; Milena Moretti; Anton N M Schoffelmeer; August B Smit; Marek T Smoluch; Francesco Clementi; Sabine Spijker
Adolescent nicotine exposure transiently increases high-affinity nicotinic receptors and modulates inhibitory synaptic transmission in rat medial prefrontal cortex.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012;26(5):1810-20.
2012: David Falck; Martin Giera; Maarten Honing; Hubertus Irth; Jon S B de Vlieger; Wilfried M A Niessen; Jeroen Kool
On-line electrochemistry-bioaffinity screening with parallel HR-LC-MS for the generation and characterization of modified p38α kinase inhibitors.
Analytical and bioanalytical chemistry 2012;403(2):367-75.
2011: Jeroen Kool; Rob Leurs; Henk Lingeman; Iwan J P De Esch; Gerdien de Kloe; Ewald Edink; Ferry Heus; Hubertus Irth; August B Smit; Wilfried M A Niessen
High-resolution bioactivity profiling of mixtures toward the acetylcholine binding protein using a nanofractionation spotter technology.
Journal of biomolecular screening 2011;16(8):917-24.
2011: Jeroen Kool; Hubertus Irth; Niels Jonker; Wilfried M A Niessen
Studying protein-protein affinity and immobilized ligand-protein affinity interactions using MS-based methods.
Analytical and bioanalytical chemistry 2011;401(4):1109-25.
2011: Jon S B de Vlieger; David Falck; Martin Giera; Mark J N Giezen; Maarten Honing; Hubertus Irth; Jeroen Kool; Henk Lingeman; Cornelis Tump; Fred van Heuveln; Jaap Wieling; Wilfried M A Niessen
High temperature liquid chromatography hyphenated with ESI-MS and ICP-MS detection for the structural characterization and quantification of halogen containing drug metabolites.
Analytica chimica acta 2011;698(1-2):69-76.
2011: Cornelius T Martha; Jan-Carel Hoogendoorn; Hubertus Irth; Wilfried M A Niessen
Optimization of information content in a mass spectrometry based flow-chemistry system by investigating different ionization approaches.
Talanta 2011;84(3):623-31.
2011: A Kretschmer; Martin Giera; Maikel Wijtmans; L de Vries; Henk Lingeman; Hubertus Irth; Wilfried M A Niessen
Derivatization of carboxylic acids with 4-APEBA for detection by positive-ion LC-ESI-MS(/MS) applied for the analysis of prostanoids and NSAID in urine.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2011;879(17-18):1393-401.
2011: Lygia Azevedo Marques; Ismail Maada; Frans J J de Kanter; Henk Lingeman; Hubertus Irth; Wilfried M A Niessen; Martin Giera
Stability-indicating study of the anti-Alzheimer's drug galantamine hydrobromide.
Journal of pharmaceutical and biomedical analysis 2011;55(1):85-92.
2011: L Switzar; Martin Giera; Henk Lingeman; Hubertus Irth; Wilfried M A Niessen
Protein digestion optimization for characterization of drug-protein adducts using response surface modeling.
Journal of chromatography. A 2011;1218(13):1715-23.
2011: Jeroen Kool; Martin Giera; Hubertus Irth; Wilfried M A Niessen
Advances in mass spectrometry-based post-column bioaffinity profiling of mixtures.
Analytical and bioanalytical chemistry 2011;399(8):2655-68.
2011: Niels Jonker; Jeroen Kool; Hubertus Irth; Wilfried M A Niessen
Recent developments in protein-ligand affinity mass spectrometry.
Analytical and bioanalytical chemistry 2011;399(8):2669-81.
2011: Jeroen Kool; Gerdien de Kloe; Arnoud D Denker; Klaas Van Altena; Marek Smoluch; Dick van Iperen; Tariq T Nahar; Rob J Limburg; Wilfried M A Niessen; Henk Lingeman; Rob Leurs; Iwan J P De Esch; August B Smit; Hubertus Irth
Nanofractionation spotter technology for rapid contactless and high-resolution deposition of LC eluent for further off-line analysis.
Analytical chemistry 2011;83(1):125-32.
2010: Ferry Heus; Martin Giera; Gerdien E de Kloe; Dick van Iperen; Joost B Buijs; Tariq T Nahar; August B Smit; Henk Lingeman; Iwan J P De Esch; Wilfried M A Niessen; Hubertus Irth; Jeroen Kool
Development of a microfluidic confocal fluorescence detection system for the hyphenation of nano-LC to on-line biochemical assays.
Analytical and bioanalytical chemistry 2010;398(7-8):3023-32.
2010: Kim Retra; Matthis Geitmann; Jeroen Kool; August B Smit; Iwan J P De Esch; Helena Danielson; Hubertus Irth
Development of surface plasmon resonance biosensor assays for primary and secondary screening of acetylcholine binding protein ligands.
Analytical biochemistry 2010;407(1):58-64.
2010: Lygia Azevedo Marques; Jeroen Kool; Frans J J de Kanter; Henk Lingeman; Wilfried Niessen; Hubertus Irth
Production and on-line acetylcholinesterase bioactivity profiling of chemical and biological degradation products of tacrine.
Journal of pharmaceutical and biomedical analysis 2010;53(3):609-16.
2010: Cornelius T Martha; Willem-Jan van Zeist; F Matthias Bickelhaupt; Hubertus Irth; Wilfried M A Niessen
Tandem mass spectrometry of silver-adducted ferrocenyl catalyst complexes.
Journal of mass spectrometry : JMS 2010;45(11):1332-43.
2010: Gerdien E de Kloe; Kim Retra; Matthis Geitmann; Per Kallblad; Tariq Nahar; René Van Elk; August B Smit; Jacqueline van Muijlwijk-Koezen; Rob Leurs; Hubertus Irth; Helena Danielson; Iwan J P De Esch
Surface plasmon resonance biosensor based fragment screening using acetylcholine binding protein identifies ligand efficiency hot spots (LE hot spots) by deconstruction of nicotinic acetylcholine receptor α7 ligands.
Journal of medicinal chemistry 2010;53(19):7192-201.
2010: David Falck; Jon S B de Vlieger; Wilfried M A Niessen; Jeroen Kool; Maarten Honing; Martin Giera; Hubertus Irth
Development of an online p38α mitogen-activated protein kinase binding assay and integration of LC-HR-MS.
Analytical and bioanalytical chemistry 2010;398(4):1771-80.
2010: Masakazu Kobayashi; Kim Retra; Francis Figaroa; Johan G Hollander; Eiso Ab; Robert J Heetebrij; Hubertus Irth; Gregg Siegal
Target immobilization as a strategy for NMR-based fragment screening: comparison of TINS, STD, and SPR for fragment hit identification.
Journal of biomolecular screening 2010;15(8):978-89.
2010: Jeroen Kool; Henk Lingeman; Wilfried Niessen; Hubertus Irth
High throughput screening methodologies classified for major drug target classes according to target signaling pathways.
Combinatorial chemistry & high throughput screening 2010;13(6):548-61.
2010: Jeroen Kool; Gerdien E de Kloe; Ben Bruyneel; Jon S B de Vlieger; Kim Retra; Maikel Wijtmans; Rene van Elk; August B Smit; Rob Leurs; Henk Lingeman; Iwan J P De Esch; Hubertus Irth
Online fluorescence enhancement assay for the acetylcholine binding protein with parallel mass spectrometric identification.
Journal of medicinal chemistry 2010;53(12):4720-30.
2010: Lygia Azevedo Marques; Martin Giera; Frans J J de Kanter; Wilfried M A Niessen; Henk Lingeman; Hubertus Irth
Photohuperzine A-A new photoisomer of huperzine A: structure elucidation, formation kinetics and activity assessment.
Journal of pharmaceutical and biomedical analysis 2010;52(2):190-4.
2010: Mark Eggink; Maikel Wijtmans; Ansgar Kretschmer; Jeroen Kool; Henk Lingeman; Iwan J P De Esch; Wilfried M A Niessen; Hubertus Irth
Targeted LC-MS derivatization for aldehydes and carboxylic acids with a new derivatization agent 4-APEBA.
Analytical and bioanalytical chemistry 2010;397(2):665-75.
2010: Jon S B de Vlieger; Ard J Kolkman; Kirsten A M Ampt; Jan N M Commandeur; Nico P E Vermeulen; Jeroen Kool; Sybren S Wijmenga; Wilfried M A Niessen; Hubertus Irth; Maarten Honing
Determination and identification of estrogenic compounds generated with biosynthetic enzymes using hyphenated screening assays, high resolution mass spectrometry and off-line NMR.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2010;878(7-8):667-74.
2009: Mark Eggink; Ségolène Charret; Maikel Wijtmans; Henk Lingeman; Jeroen Kool; Wilfried M A Niessen; Hubertus Irth
Development of an on-line weak-cation exchange liquid chromatography-tandem mass spectrometric method for screening aldehyde products in biological matrices.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2009;877(31):3937-45.
2009: Cornelius T Martha; AE Heemskerk; Jan-Carel Hoogendoorn; Niels Elders; Wilfried M A Niessen; Romano V A Orru; Hubertus Irth
High-throughput reaction optimisation and activity screening of ferrocene-based Lewis acid-catalyst complexes by using continuous-flow reaction detection mass spectrometry.
Chemistry (Weinheim an der Bergstrasse, Germany) 2009;15(30):7368-75.
2009: Martin Giera; Ferry Heus; Laura Janssen; Jeroen Kool; Henk Lingeman; Hubertus Irth
Microfractionation revisited: a 1536 well high resolution screening assay.
Analytical chemistry 2009;81(13):5460-6.
2009: Nils Helge Schebb; Helene Faber; Ronald Maul; Ferry Heus; Jeroen Kool; Hubertus Irth; Uwe Karst
Analysis of glutathione adducts of patulin by means of liquid chromatography (HPLC) with biochemical detection (BCD) and electrospray ionization tandem mass spectrometry (ESI-MS/MS).
Analytical and bioanalytical chemistry 2009;394(5):1361-73.
2009: N Jonker; A Kretschmer; Jeroen Kool; A Fernandez; D Kloos; Johannes G Krabbe; Henk Lingeman; Hubertus Irth
Online magnetic bead dynamic protein-affinity selection coupled to LC-MS for the screening of pharmacologically active compounds.
Analytical chemistry 2009;81(11):4263-70.
2008: Mark Eggink; Maikel Wijtmans; Reggy Ekkebus; Henk Lingeman; Iwan J P De Esch; Jeroen Kool; Wilfried M A Niessen; Hubertus Irth
Development of a selective ESI-MS derivatization reagent: synthesis and optimization for the analysis of aldehydes in biological mixtures.
Analytical chemistry 2008;80(23):9042-51.
2008: Micaela C Damsten; Jon S B de Vlieger; Wilfried M A Niessen; Hubertus Irth; Nico P E Vermeulen; Jan N M Commandeur
Trimethoprim: novel reactive intermediates and bioactivation pathways by cytochrome p450s.
Chemical research in toxicology 2008;21(11):2181-7.
2008: Gerardo R Marchesini; Anastasia Meimaridou; Willem Haasnoot; Eline Meulenberg; Faywell Albertus; Mineyuki Mizuguchi; Makoto Takeuchi; Hubertus Irth; Albertinka J Murk
Biosensor discovery of thyroxine transport disrupting chemicals.
Toxicology and applied pharmacology 2008;232(1):150-60.
2008: Niels Jonker; Jeroen Kool; Johannes G Krabbe; Kim Retra; Henk Lingeman; Hubertus Irth
Screening of protein-ligand interactions using dynamic protein-affinity chromatography solid-phase extraction-liquid chromatography-mass spectrometry.
Journal of chromatography. A 2008;1205(1-2):71-7.
2008: Cornelius T Martha; Niels Elders; Johannes G Krabbe; Jeroen Kool; Wilfried M A Niessen; Romano V A Orru; Hubertus Irth
Online screening of homogeneous catalyst performance using reaction detection mass spectrometry.
Analytical chemistry 2008;80(18):7121-7.
2008: Nils Helge Schebb; Ferry Heus; Thorsten Saenger; Uwe Karst; Hubertus Irth; Jeroen Kool
Development of a countergradient parking system for gradient liquid chromatography with online biochemical detection of serine protease inhibitors.
Analytical chemistry 2008;80(17):6764-72.
2008: Jeroen Carol-Visser; Marcel van der Schans; Alex Fidder; Albert G Hulst; Ben L M van Baar; Hubertus Irth; Daan Noort
Development of an automated on-line pepsin digestion-liquid chromatography-tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2008;870(1):91-7.
2008: Mark Eggink; Wilmar Romero; René J J Vreuls; Henk Lingeman; Wilfried M A Niessen; Hubertus Irth
Development and optimization of a system for comprehensive two-dimensional liquid chromatography with UV and mass spectrometric detection for the separation of complex samples by multi-step gradient elution.
Journal of chromatography. A 2008;1188(2):216-26.
2008: Lawrence Akoto; Frans Stellaard; Hubertus Irth; René J J Vreuls; Roel Pel
Improved fatty acid detection in micro-algae and aquatic meiofauna species using a direct thermal desorption interface combined with comprehensive gas chromatography-time-of-flight mass spectrometry.
Journal of chromatography. A 2008;1186(1-2):254-61.
2008: Lawrence Akoto; René J J Vreuls; Hubertus Irth; Virgilio Floris; Hans Hoogveld; Roel Pel
Determination of the carbon isotopic composition of whole/intact biological specimens using at-line direct thermal desorption to effect thermally assisted hydrolysis/methylation.
Journal of chromatography. A 2008;1186(1-2):372-9.
2008: Lawrence Akoto; René J J Vreuls; Hubertus Irth; Roel Pel; Frans Stellaard
Fatty acid profiling of raw human plasma and whole blood using direct thermal desorption combined with gas chromatography-mass spectrometry.
Journal of chromatography. A 2008;1186(1-2):365-71.
2007: Jeroen Kool; André van Marle; Saskia Hulscher; Maurice Selman; Dick J van Iperen; Klaas Van Altena; Michel Gillard; Remko A Bakker; Hubertus Irth; Rob Leurs; Nico P E Vermeulen
A flow-through fluorescence polarization detection system for measuring GPCR-mediated modulation of cAMP production.
Journal of biomolecular screening 2007;12(8):1074-83.
2007: Sebastiaan M van Liempd; Jeroen Kool; John Meerman; Hubertus Irth; Nico P Vermeulen
Metabolic profiling of endocrine-disrupting compounds by on-line cytochrome p450 bioreaction coupled to on-line receptor affinity screening.
Chemical research in toxicology 2007;20(12):1825-32.
2007: Johannes S Hoos; Micaela C Damsten; Jon S B de Vlieger; Jan N M Commandeur; Nico P E Vermeulen; Wilfried M A Niessen; Henk Lingeman; Hubertus Irth
Automated detection of covalent adducts to human serum albumin by immunoaffinity chromatography, on-line solution phase digestion and liquid chromatography-mass spectrometry.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2007;859(2):147-56.
2007: Jeroen Kool; Sebastiaan M van Liempd; Stefan Harmsen; Joran Beckman; Danny A van Elswijk; Jan N M Commandeur; Hubertus Irth; Nico P E Vermeulen
Cytochrome P450 bio-affinity detection coupled to gradient HPLC: on-line screening of affinities to cytochrome P4501A2 and 2D6.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2007;858(1-2):49-58.
2007: Jeroen Kool; Sebastiaan M van Liempd; Stefan Harmsen; Tim Schenk; Hubertus Irth; Jan N M Commandeur; Nico P E Vermeulen
An on-line post-column detection system for the detection of reactive-oxygen-species-producing compounds and antioxidants in mixtures.
Analytical and bioanalytical chemistry 2007;388(4):871-9.
2007: Gerardo R Marchesini; K Koopal; E Meulenberg; Willem Haasnoot; Hubertus Irth
Spreeta-based biosensor assays for endocrine disruptors.
Biosensors & bioelectronics 2007;22(9-10):1908-15.
2007: Jeroen Kool; Mark Eggink; Huub van Rossum; Sebastiaan M van Liempd; Danny A van Elswijk; Hubertus Irth; Jan N M Commandeur; John Meerman; Nico P E Vermeulen
Online biochemical detection of glutathione-S-transferase P1-specific inhibitors in complex mixtures.
Journal of biomolecular screening 2007;12(3):396-405.
2007: Jeroen Kool; Sebastiaan M van Liempd; Huub van Rossum; Danny A van Elswijk; Hubertus Irth; Jan N M Commandeur; Nico P E Vermeulen
Development of three parallel cytochrome P450 enzyme affinity detection systems coupled on-line to gradient high-performance liquid chromatography.
Drug metabolism and disposition: the biological fate of chemicals 2007;35(4):640-8.
2007: Johannes G Krabbe; Arjen R de Boer; Gert van der Zwan; Henk Lingeman; Wilfried M A Niessen; Hubertus Irth
Metal-complex formation in continuous-flow ligand-exchange reactors studied by electrospray mass spectrometry.
Journal of the American Society for Mass Spectrometry 2007;18(4):707-13.
2007: Ben Bruyneel; Johannes S Hoos; Marek Smoluch; Henk Lingeman; Wilfried M A Niessen; Hubertus Irth
Trace analysis of proteins using postseparation solution-phase digestion and electrospray mass spectrometric detection of marker peptides.
Analytical chemistry 2007;79(4):1591-8.
2006: Johannes G Krabbe; F Gao; J Li; J Eric Ahlskog; Henk Lingeman; Wilfried M A Niessen; Hubertus Irth
Selective detection and identification of phosphorylated proteins by simultaneous ligand-exchange fluorescence detection and mass spectrometry.
Journal of chromatography. A 2006;1130(2):287-95.
2006: Sebastiaan M van Liempd; Jeroen Kool; Wilfried M A Niessen; D E van Elswijk; Hubertus Irth; Nico P E Vermeulen
On-line formation, separation, and estrogen receptor affinity screening of cytochrome P450-derived metabolites of selective estrogen receptor modulators.
Drug metabolism and disposition: the biological fate of chemicals 2006;34(9):1640-9.
2006: Jeroen Kool; Rawi Ramautar; Sebastiaan M van Liempd; Joran Beckman; Frans J J de Kanter; John Meerman; Tim Schenk; Hubertus Irth; Jan N M Commandeur; Nico P E Vermeulen
Rapid on-line profiling of estrogen receptor binding metabolites of tamoxifen.
Journal of medicinal chemistry 2006;49(11):3287-92.
2006: Camiel F de Jong; Rico J E Derks; Ben Bruyneel; Wilfried Niessen; Hubertus Irth
High-performance liquid chromatography-mass spectrometry-based acetylcholinesterase assay for the screening of inhibitors in natural extracts.
Journal of chromatography. A 2006;1112(1-2):303-10.
2006: Thomas Letzel; Rico J E Derks; Cornelius T Martha; André van Marle; Hubertus Irth
Sensitive determination of G-protein-coupled receptor binding ligands by solid phase extraction-electrospray ionization-mass spectrometry.
Journal of pharmaceutical and biomedical analysis 2006;40(3):744-51.
2006: Gerardo R Marchesini; Eline Meulenberg; Willem Haasnoot; Mineyuki Mizuguchi; Hubertus Irth
Biosensor recognition of thyroid-disrupting chemicals using transport proteins.
Analytical chemistry 2006;78(4):1107-14.
2006: Johannes S Hoos; Hilke Sudergat; Jens-Peter Hoelck; Mark Stahl; Jon S B de Vlieger; Wilfried M A Niessen; Henk Lingeman; Hubertus Irth
Selective quantitative bioanalysis of proteins in biological fluids by on-line immunoaffinity chromatography-protein digestion-liquid chromatography-mass spectrometry.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2006;830(2):262-9.
2005: Arjen R de Boer; Juan M Alcaide-Hidalgo; Johannes G Krabbe; Jeffrey Kolkman; Caspar N van Emde Boas; Wilfried M A Niessen; Henk Lingeman; Hubertus Irth
High-temperature liquid chromatography coupled on-line to a continuous-flow biochemical screening assay with electrospray ionization mass spectrometric detection.
Analytical chemistry 2005;77(24):7894-900.
2005: Johannes G Krabbe; Henk Lingeman; Wilfried M A Niessen; Hubertus Irth
Screening for metal ligands by liquid chromatography--ligand-exchange--electrospray mass spectrometry.
Journal of chromatography. A 2005;1093(1-2):36-46.
2005: N Nuengchamnong; C F de Jong; Ben Bruyneel; Wilfried M A Niessen; Hubertus Irth; Kornkanok Ingkaninan
HPLC coupled on-line to ESI-MS and a DPPH-based assay for the rapid identification of anti-oxidants in Butea superba.
Phytochemical analysis : PCA 2005;16(6):422-8.
2005: Arjen R de Boer; Ben Bruyneel; Johannes G Krabbe; Henk Lingeman; Wilfried M A Niessen; Hubertus Irth
A microfluidic-based enzymatic assay for bioactivity screening combined with capillary liquid chromatography and mass spectrometry.
Lab on a chip 2005;5(11):1286-92.
2005: Jeroen Carol; Maarten C J K Gorseling; Camiel F de Jong; Henk Lingeman; Charles E Kientz; Ben L M van Baar; Hubertus Irth
Determination of denaturated proteins and biotoxins by on-line size-exclusion chromatography-digestion-liquid chromatography-electrospray mass spectrometry.
Analytical biochemistry 2005;346(1):150-7.
2005: Jeroen Kool; Sebastiaan M van Liempd; Rawi Ramautar; Tim Schenk; John Meerman; Hubertus Irth; Jan N M Commandeur; Nico P E Vermeulen
Development of a novel cytochrome p450 bioaffinity detection system coupled online to gradient reversed-phase high-performance liquid chromatography.
Journal of biomolecular screening 2005;10(5):427-36.
2005: Junko Hirata; Lai Ping Chung; Freek Ariese; Hubertus Irth; Cees Gooijer
Coupling of size-exclusion chromatography to a continuous assay for subtilisin using a fluorescence resonance energy transfer peptide substrate: testing of two standard inhibitors.
Journal of chromatography. A 2005;1081(2):140-4.
2005: N F C Visser; Henk Lingeman; Hubertus Irth
Sample preparation for peptides and proteins in biological matrices prior to liquid chromatography and capillary zone electrophoresis.
Analytical and bioanalytical chemistry 2005;382(3):535-58.
2005: Sebastiaan M van Liempd; Jeroen Kool; Jelle Reinen; Tim Schenk; John Meerman; Hubertus Irth; Nico P E Vermeulen
Development and validation of a microsomal online cytochrome P450 bioreactor coupled to solid-phase extraction and reversed-phase liquid chromatography.
Journal of chromatography. A 2005;1075(1-2):205-12.
2005: Arjen R de Boer; Thomas Letzel; Henk Lingeman; Hubertus Irth
Systematic development of an enzymatic phosphorylation assay compatible with mass spectrometric detection.
Analytical and bioanalytical chemistry 2005;381(3):647-55.
2004: Junko Hirata; Camiel F de Jong; Maarten M van Dongen; Joost B Buijs; Freek Ariese; Hubertus Irth; Cees Gooijer
A flow injection kinase assay system based on time-resolved fluorescence resonance energy-transfer detection in the millisecond range.
Analytical chemistry 2004;76(15):4292-8.
2004: Arjen R de Boer; Thomas Letzel; Danny A van Elswijk; Henk Lingeman; Wilfried M A Niessen; Hubertus Irth
On-line coupling of high-performance liquid chromatography to a continuous-flow enzyme assay based on electrospray ionization mass spectrometry.
Analytical chemistry 2004;76(11):3155-61.
2004: Danny A van Elswijk; Uwe P Schobel; Ephraim P Lansky; Hubertus Irth; Jan van der Greef
Rapid dereplication of estrogenic compounds in pomegranate (Punica granatum) using on-line biochemical detection coupled to mass spectrometry.
Phytochemistry 2004;65(2):233-41.
2003: Johannes G Krabbe; Henk Lingeman; Wilfried M A Niessen; Hubertus Irth
Ligand-exchange detection of phosphorylated peptides using liquid chromatography electrospray mass spectrometry.
Analytical chemistry 2003;75(24):6853-60.
2003: Danny A van Elswijk; Otto Diefenbach; Sonja van der Berg; Hubertus Irth; Ubbo R Tjaden; Jan van der Greef
Rapid detection and identification of angiotensin-converting enzyme inhibitors by on-line liquid chromatography-biochemical detection, coupled to electrospray mass spectrometry.
Journal of chromatography. A 2003;1020(1):45-58.
2003: N F C Visser; M van Harmelen; Henk Lingeman; Hubertus Irth
On-line SPE-CE for the determination of insulin derivatives in biological fluids.
Journal of pharmaceutical and biomedical analysis 2003;33(3):451-62.
2003: Tim Schenk; A Molendijk; Hubertus Irth; Ubbo R Tjaden; Jan van der Greef
Liquid chromatography coupled on-line to flow cytometry for postcolumn homogeneous biochemical detection.
Analytical chemistry 2003;75(16):4272-8.
2003: Tim Schenk; G J Breel; P Koevoets; S van den Berg; AC Hogenboom; Hubertus Irth; Ubbo R Tjaden; Jan van der Greef
Screening of natural products extracts for the presence of phosphodiesterase inhibitors using liquid chromatography coupled online to parallel biochemical detection and chemical characterization.
Journal of biomolecular screening 2003;8(4):421-9.
2003: Rico J E Derks; AC Hogenboom; Gert van der Zwan; Hubertus Irth
On-line continuous-flow, multi-protein biochemical assays for the characterization of bioaffinity compounds using electrospray quadrupole time-of-flight mass spectrometry.
Analytical chemistry 2003;75(14):3376-84.
2003: N F C Visser; Henk Lingeman; Hubertus Irth
On-line SPE-RP-LC for the determination of insulin derivatives in biological matrices.
Journal of pharmaceutical and biomedical analysis 2003;32(2):295-309.
2003: In Kyung Rhee; Natalie Appels; Teus Luijendijk; Hubertus Irth; Robert Verpoorte
Determining acetylcholinesterase inhibitory activity in plant extracts using a fluorimetric flow assay.
Phytochemical analysis : PCA 2003;14(3):145-9.
2003: Tim Schenk; Natalie Appels; Danny A van Elswijk; Hubertus Irth; Ubbo R Tjaden; Jan van der Greef
A generic assay for phosphate-consuming or -releasing enzymes coupled on-line to liquid chromatography for lead finding in natural products.
Analytical biochemistry 2003;316(1):118-26.
2002: M C García; AC Hogenboom; Herman Zappey; Hubertus Irth
Effect of the mobile phase composition on the separation and detection of intact proteins by reversed-phase liquid chromatography-electrospray mass spectrometry.
Journal of chromatography. A 2002;957(2):187-99.
2001: Tim Schenk; Hubertus Irth; György Marko-Varga; Lars-Erik Edholm; Ubbo R Tjaden; Jan van der Greef
Potential of on-line micro-LC immunochemical detection in the bioanalysis of cytokines.
Journal of pharmaceutical and biomedical analysis 2001;26(5-6):975-85.
2001: U Schobel; M Frenay; Danny A van Elswijk; J M McAndrews; Kelly R Long; L M Olson; S C Bobzin; Hubertus Irth
High resolution screening of plant natural product extracts for estrogen receptor alpha and beta binding activity using an online HPLC-MS biochemical detection system.
Journal of biomolecular screening 2001;6(5):291-303.
2001: AC Hogenboom; Arjen R de Boer; Rico J E Derks; Hubertus Irth
Continuous-flow, on-line monitoring of biospecific interactions using electrospray mass spectrometry.
Analytical chemistry 2001;73(16):3816-23.
2000: Sandra A G Visser; C J Smulders; W W Gladdines; Hubertus Irth; Piet H van Der Graaf; Meindert Danhof
High-performance liquid chromatography of the neuroactive steroids alphaxalone and pregnanolone in plasma using dansyl hydrazine as fluorescent label: application to a pharmacokinetic-pharmacodynamic study in rats.
Journal of chromatography. B, Biomedical sciences and applications 2000;745(2):357-63.
2000: M Mazereeuw; CM de Best; Ubbo R Tjaden; Hubertus Irth; Jan van der Greef
Free flow electrophoresis device for continuous on-line separation in analytical systems. An application in biochemical detection.
Analytical chemistry 2000;72(16):3881-6.
2000: MR van Bommel; AP de Jong; Ubbo R Tjaden; Hubertus Irth; Jan van der Greef
High-performance liquid chromatography coupled to enzyme-amplified biochemical detection for the analysis of hemoglobin after pre-column biotinylation.
Journal of chromatography. A 2000;886(1-2):19-29.
2000: Kornkanok Ingkaninan; Arno Hazekamp; CM de Best; Hubertus Irth; Ubbo R Tjaden; Robert van der Heijden; Jan van der Greef; Robert Verpoorte
The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from narcissus 'Sir Winston Churchill'.
Journal of natural products 2000;63(6):803-6.
2000: Kornkanok Ingkaninan; CM de Best; Robert van der Heijden; AJ Hofte; B Karabatak; Hubertus Irth; Ubbo R Tjaden; Jan van der Greef; Robert Verpoorte
High-performance liquid chromatography with on-line coupled UV, mass spectrometric and biochemical detection for identification of acetylcholinesterase inhibitors from natural products.
Journal of chromatography. A 2000;872(1-2):61-73.
1999: MR van Bommel; AP de Jong; Ubbo R Tjaden; Hubertus Irth; Jan van der Greef
Enzyme amplification as detection tool in continuous-flow systems. II. On-line coupling of liquid chromatography to enzyme-amplified biochemical detection after pre-column derivatization with biotin.
Journal of chromatography. A 1999;855(2):397-409.
1999: MR van Bommel; AP de Jong; Ubbo R Tjaden; Hubertus Irth; Jan van der Greef
Enzyme amplification as detection tool in continuous-flow systems. I. Development of an enzyme-amplified biochemical detection system coupled on-line to flow-injection analysis.
Journal of chromatography. A 1999;855(2):383-96.
1998: AJ Oosterkamp; R van der Hoeven; W Glässgen; B König; Ubbo R Tjaden; Jan van der Greef; Hubertus Irth
Gradient reversed-phase liquid chromatography coupled on-line to receptor-affinity detection based on the urokinase receptor.
Journal of chromatography. B, Biomedical sciences and applications 1998;715(1):331-8.
1997: E S Lutz; Hubertus Irth; Ubbo R Tjaden; Jan van der Greef
Biochemical detection for direct bead surface analysis.
Analytical chemistry 1997;69(23):4878-84.
1997: A J Oostercamp; Hubertus Irth; Ubbo R Tjaden; Jan van der Greef
Theoretical concepts of on-line liquid chromatographic-biochemical detection systems. II. Detection systems based on labelled affinity proteins.
Journal of chromatography. A 1997;787(1-2):37-46.
1997: AJ Oosterkamp; Hubertus Irth; M T Villaverde Herraiz; Ubbo R Tjaden; Jan van der Greef
Theoretical concepts of on-line liquid chromatographic-biochemical detection systems. I. Detection systems based on labelled ligands.
Journal of chromatography. A 1997;787(1-2):27-35.
1997: R A van der Hoeven; AJ Hofte; M Frenay; Hubertus Irth; Ubbo R Tjaden; Jan van der Greef; A Rudolphi; K S Boos; György Marko-Varga; Lars-Erik Edholm
Liquid chromatography-mass spectrometry with on-line solid-phase extraction by a restricted-access C18 precolumn for direct plasma and urine injection.
Journal of chromatography. A 1997;762(1-2):193-200.
1996: E S Lutz; Hubertus Irth; Ubbo R Tjaden; Jan van der Greef
Applying hollow fibres for separating free and bound label in continuous-flow immunochemical detection.
Journal of chromatography. A 1996;755(2):179-87.
1996: A J Oosterkamp; Hubertus Irth; L Heintz; György Marko-Varga; Ubbo R Tjaden; Jan van der Greef
Simultaneous determination of cross-reactive leukotrienes in biological matrices using on-line liquid chromatography immunochemical detection.
Analytical chemistry 1996;68(23):4101-6.
1996: A J Oosterkamp; M T Villaverde Herraiz; Hubertus Irth; Ubbo R Tjaden; Jan van der Greef
Reversed-phase liquid chromatography coupled on-line to receptor affinity detection based on the human estrogen receptor.
Analytical chemistry 1996;68(7):1201-6.
1995: B A Buscher; Ubbo R Tjaden; Hubertus Irth; E M Andersson; Jan van der Greef
Determination of 1,2,6-inositol trisphosphate (derivatives) in plasma using iron(III)-loaded adsorbents and capillary zone electrophoresis-(indirect) UV detection.
Journal of chromatography. A 1995;718(2):413-9.
1994: Hubertus Irth; Anne-Marie Cleton; R A Mathôt; Meindert Danhof; Adriaan P IJzerman; Ubbo R Tjaden; Jan van der Greef
Liquid chromatographic determination of the adenosine receptor agonist CGS 21680 in blood using on-line solid-phase extraction on a phenylboronic acid support and fluorescence detection.
Journal of chromatography. B, Biomedical applications 1994;658(1):207-12.
1994: E van der Vlis; Hubertus Irth; Ubbo R Tjaden; Jan van der Greef
Potential of receptor-ligand interactions for sample handling in liquid and gas chromatography.
Journal of chromatography. A 1994;665(2):233-41.
1994: A J Oosterkamp; Hubertus Irth; M Beth; K K Unger; Ubbo R Tjaden; J van de Greef
Bioanalysis of digoxin and its metabolites using direct serum injection combined with liquid chromatography and on-line immunochemical detection.
Journal of chromatography. B, Biomedical applications 1994;653(1):55-61.
1993: H J Reeuwijk; Hubertus Irth; Ubbo R Tjaden; F W Merkus; Jan van der Greef
Liquid chromatographic determination of beta-cyclodextrin derivatives based on fluorescence enhancement after inclusion complexation.
Journal of chromatography 1993;614(1):95-100.
1992: N J Reinhoud; Ubbo R Tjaden; Hubertus Irth; Jan van der Greef
Bioanalysis of some anthracyclines in human plasma by capillary electrophoresis with laser-induced fluorescence detection.
Journal of chromatography 1992;574(2):327-34.
1992: V Venizelos; Hubertus Irth; Ubbo R Tjaden; Jan van der Greef; Douwe D Breimer; T M Mulders; Gerard J Mulder
Enantioselective determination of R- and S-(alpha-bromoisovaleryl)urea in plasma using high-performance liquid chromatography after solid-phase extraction.
Journal of chromatography 1992;573(2):259-64.
1991: A Marinelli; Jan Hein van Dierendonck; G M van Brakel; Hubertus Irth; Peter Kuppen; U R Tjaden; Cornelis J H van de Velde
Increasing the effective concentration of melphalan in experimental rat liver tumours: comparison of isolated liver perfusion and hepatic artery infusion.
British journal of cancer 1991;64(6):1069-75.
1991: D S Stegehuis; Hubertus Irth; Ubbo R Tjaden; Jan van der Greef
Isotachophoresis as an on-line concentration pretreatment technique in capillary electrophoresis.
Journal of chromatography 1991;538(2):393-402.