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Makkuni Jayaram

This is a preview profile on BiomedExperts - the first literature-based scientific social network. It brings the right researchers together and allows them to collaborate online. Collexis and Dell provide the BiomedExperts network of +1.5 Million pre-calculated profiles free of charge to researchers worldwide.

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Co-Publications
Name
20
Morimoto, Asuka
16
Harshey, Rasika
12
Velmurugan, Soundarapandian
11
Voziyanov, Yuri
8
Grainge, Ian
7
Mehta, Shwetal
7
Pathania, Shailja
5
Yang, Xian-Mei
5
Knudsen, Birgitta
4
Araki, Hiroyuki
4
Ahn, YT
4
Westergaard, Ole
4
Uzri, Dina
3
Namgoong, SY
3
Paek, Andrew

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Publications

TOP 3
Kachroo Aashiq H; Jayaram Makkuni; Rowley Paul A
Metabolic engineering without plasmids.
Ma Chien-Hui; Rowley Paul A; Macieszak Anna; Guga Piotr; Jayaram Makkuni
Active site electrostatics protect genome integrity by blocking abortive hydrolysis during DNA recombination.
Cui Hong; Ghosh Santanu K; Jayaram Makkuni
The selfish yeast plasmid uses the nuclear motor Kip1p but not Cin8p for its localization and equal segregation.

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All Publications

2009: Kachroo Aashiq H; Jayaram Makkuni; Rowley Paul A
Metabolic engineering without plasmids.
Nature biotechnology 2009;27(8):729-31.
2009: Ma Chien-Hui; Rowley Paul A; Macieszak Anna; Guga Piotr; Jayaram Makkuni
Active site electrostatics protect genome integrity by blocking abortive hydrolysis during DNA recombination.
The EMBO journal 2009;28(12):1745-56.
2009: Cui Hong; Ghosh Santanu K; Jayaram Makkuni
The selfish yeast plasmid uses the nuclear motor Kip1p but not Cin8p for its localization and equal segregation.
The Journal of cell biology 2009;185(2):251-64.
2009: Ma Chien-Hui; Kachroo Aashiq H; Macieszak Anna; Chen Tzu-Yang; Guga Piotr; Jayaram Makkuni
Reactions of Cre with methylphosphonate DNA: similarities and contrasts with Flp and vaccinia topoisomerase.
PloS one 2009;4(9):e7248.
2007: Yin Zhiqi; Suzuki Asaka; Lou Zheng; Jayaram Makkuni; Harshey Rasika M
Interactions of phage Mu enhancer and termini that specify the assembly of a topologically unique interwrapped transpososome.
Journal of molecular biology 2007;372(2):382-96.
2007: Ghosh Santanu K; Hajra Sujata; Jayaram Makkuni
Faithful segregation of the multicopy yeast plasmid through cohesin-mediated recognition of sisters.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(32):13034-9.
2007: Jayaram Makkuni
Split target specificity of ResT: a design for protein delivery, site selectivity and regulation of enzyme activity?
Molecular microbiology 2007;64(3):575-9.
2007: Ma Chien-Hui; Kwiatek Agnieszka; Bolusani Swetha; Voziyanov Yuri; Jayaram Makkuni
Unveiling hidden catalytic contributions of the conserved His/Trp-III in tyrosine recombinases: assembly of a novel active site in Flp recombinase harboring alanine at this position.
Journal of molecular biology 2007;368(1):183-96.
2007: Du Quan; Livshits Alexei; Kwiatek Agnieszka; Jayaram Makkuni; Vologodskii Alexander
Protein-induced local DNA bends regulate global topology of recombination products.
Journal of molecular biology 2007;368(1):170-82.
2006: Harshey Rasika M; Jayaram Makkuni
The mu transpososome through a topological lens.
Critical reviews in biochemistry and molecular biology 2006;41(6):387-405.
2006: Hajra Sujata; Ghosh Santanu Kumar; Jayaram Makkuni
The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-microm circle partitioning locus and promotes equal plasmid segregation.
The Journal of cell biology 2006;174(6):779-90.
2006: Bolusani Swetha; Ma Chien-Hui; Paek Andrew; Konieczka Jay H; Jayaram Makkuni; Voziyanov Yuri
Evolution of variants of yeast site-specific recombinase Flp that utilize native genomic sequences as recombination target sites.
Nucleic acids research 2006;34(18):5259-69.
2006: Ghosh Santanu Kumar; Hajra Sujata; Paek Andrew; Jayaram Makkuni
Mechanisms for chromosome and plasmid segregation.
Annual review of biochemistry 2006;75():211-41.
2005: Dobson Melanie J; Pickett Andrew J; Velmurugan Soundarapandian; Pinder Jordan B; Barrett Lori A; Jayaram Makkuni; Chew Joyce S K
The 2 microm plasmid causes cell death in Saccharomyces cerevisiae with a mutation in Ulp1 protease.
Molecular and cellular biology 2005;25(10):4299-310.
2005: Mehta Shwetal; Yang Xian-Mei; Jayaram Makkuni; Velmurugan Soundarapandian
A novel role for the mitotic spindle during DNA segregation in yeast: promoting 2 microm plasmid-cohesin association.
Molecular and cellular biology 2005;25(10):4283-98.
2005: Yin Zhiqi; Jayaram Makkuni; Pathania Shailja; Harshey Rasika M
The Mu transposase interwraps distant DNA sites within a functional transpososome in the absence of DNA supercoiling.
The Journal of biological chemistry 2005;280(7):6149-56.
2004: Yang Xian-Mei; Mehta Shwetal; Uzri Dina; Jayaram Makkuni; Velmurugan Soundarapandian
Mutations in a partitioning protein and altered chromatin structure at the partitioning locus prevent cohesin recruitment by the Saccharomyces cerevisiae plasmid and cause plasmid missegregation.
Molecular and cellular biology 2004;24(12):5290-303.
2004: Konieczka Jay H; Paek Andrew; Jayaram Makkuni; Voziyanov Yuri
Recombination of hybrid target sites by binary combinations of Flp variants: mutations that foster interprotomer collaboration and enlarge substrate tolerance.
Journal of molecular biology 2004;339(2):365-78.
2004: Jayaram Makkuni; Mehta Shwetal; Uzri Dina; Velmurugan Soundarapandian
Segregation of the yeast plasmid: similarities and contrasts with bacterial plasmid partitioning.
Plasmid 2004;51(3):162-78.
2004: Jayaram Makkuni; Mehta Shwetal; Uzri Dina; Voziyanov Yuri; Velmurugan Soundarapandian
Site-specific recombination and partitioning systems in the stable high copy propagation of the 2-micron yeast plasmid.
Progress in nucleic acid research and molecular biology 2004;77():127-72.
2003: Velmurugan Soundarapandian; Mehta Shwetal; Uzri Dina; Jayaram Makkuni
Stable propagation of 'selfish' genetic elements.
Journal of biosciences 2003;28(5):623-36.
2003: Pathania Shailja; Jayaram Makkuni; Harshey Rasika M
A unique right end-enhancer complex precedes synapsis of Mu ends: the enhancer is sequestered within the transpososome throughout transposition.
The EMBO journal 2003;22(14):3725-36.
2003: Voziyanov Yuri; Konieczka Jay H; Stewart A Francis; Jayaram Makkuni
Stepwise manipulation of DNA specificity in Flp recombinase: progressively adapting Flp to individual and combinatorial mutations in its target site.
Journal of molecular biology 2003;326(1):65-76.
2003: Velmurugan Soundarapandian; Mehta Shwetal; Jayaram Makkuni
Selfishness in moderation: evolutionary success of the yeast plasmid.
Current topics in developmental biology 2003;56():1-24.
2002: Mehta Shwetal; Yang Xian Mei; Chan Clarence S; Dobson Melanie J; Jayaram Makkuni; Velmurugan Soundarapandian
The 2 micron plasmid purloins the yeast cohesin complex: a mechanism for coupling plasmid partitioning and chromosome segregation?
The Journal of cell biology 2002;158(4):625-37.
2002: Grainge Ian; Pathania Shailja; Vologodskii Alexander; Harshey Rasika M; Jayaram Makkuni
Symmetric DNA sites are functionally asymmetric within Flp and Cre site-specific DNA recombination synapses.
Journal of molecular biology 2002;320(3):515-27.
2002: Pathania Shailja; Jayaram Makkuni; Harshey Rasika M
Path of DNA within the Mu transpososome. Transposase interactions bridging two Mu ends and the enhancer trap five DNA supercoils.
Cell 2002;109(4):425-36.
2002: Voziyanov Yuri; Stewart A Francis; Jayaram Makkuni
A dual reporter screening system identifies the amino acid at position 82 in Flp site-specific recombinase as a determinant for target specificity.
Nucleic acids research 2002;30(7):1656-63.
2001: Grainge I; Lee J; Xu C J; Jayaram M
DNA recombination and RNA cleavage activities of the Flp protein: roles of two histidine residues in the orientation and activation of the nucleophile for strand cleavage.
Journal of molecular biology 2001;314(4):717-33.
2001: Sau A K; DeVue Tribble G; Grainge I; Frohlich R F; Knudsen B R; Jayaram M
Biochemical and kinetic analysis of the RNase active sites of the integrase/tyrosine family site-specific DNA recombinases.
The Journal of biological chemistry 2001;276(49):46612-23.
2001: Lisby M; Olesen J R; Skouboe C; Krogh B O; Straub T; Boege F; Velmurugan S; Martensen P M; Andersen A H; Jayaram M; Westergaard O; Knudsen B R
Residues within the N-terminal domain of human topoisomerase I play a direct role in relaxation.
The Journal of biological chemistry 2001;276(23):20220-7.
2001: Frøhlich R F; Hansen S G; Lisby M; Grainge I; Westergaard O; Jayaram M; Knudsen B R
Inhibition of Flp recombinase by the topoisomerase I-targeting drugs, camptothecin and NSC-314622.
The Journal of biological chemistry 2001;276(10):6993-7.
2000: Tribble G; Ahn Y T; Lee J; Dandekar T; Jayaram M
DNA recognition, strand selectivity, and cleavage mode during integrase family site-specific recombination.
The Journal of biological chemistry 2000;275(29):22255-67.
2000: Grainge I; Buck D; Jayaram M
Geometry of site alignment during int family recombination: antiparallel synapsis by the Flp recombinase.
Journal of molecular biology 2000;298(5):749-64.
2000: Velmurugan S; Yang X M; Chan C S; Dobson M; Jayaram M
Partitioning of the 2-microm circle plasmid of Saccharomyces cerevisiae. Functional coordination with chromosome segregation and plasmid-encoded rep protein distribution.
The Journal of cell biology 2000;149(3):553-66.
2000: Lee J; Tribble G; Jayaram M
Resolution of tethered antiparallel and parallel holliday junctions by the Flp site-specific recombinase.
Journal of molecular biology 2000;296(2):403-19.
1999: Grainge I; Jayaram M
The integrase family of recombinase: organization and function of the active site.
Molecular microbiology 1999;33(3):449-56.
1999: Voziyanov Y; Pathania S; Jayaram M
A general model for site-specific recombination by the integrase family recombinases.
Nucleic acids research 1999;27(4):930-41.
1999: Lee J; Jayaram M; Grainge I
Wild-type Flp recombinase cleaves DNA in trans.
The EMBO journal 1999;18(3):784-91.
1998: Velmurugan S; Ahn Y T; Yang X M; Wu X L; Jayaram M
The 2 micrometer plasmid stability system: analyses of the interactions among plasmid- and host-encoded components.
Molecular and cellular biology 1998;18(12):7466-77.
1998: Xu C J; Ahn Y T; Pathania S; Jayaram M
Flp ribonuclease activities. Mechanistic similarities and contrasts to site-specific DNA recombination.
The Journal of biological chemistry 1998;273(46):30591-8.
1998: Knudsen B R; Lee J; Lisby M; Westergaard O; Jayaram M
Alcoholysis and strand joining by the Flp site-specific recombinase. Mechanistically equivalent reactions mediated by distinct catalytic configurations.
The Journal of biological chemistry 1998;273(34):22028-36.
1998: Xu C J; Grainge I; Lee J; Harshey R M; Jayaram M
Unveiling two distinct ribonuclease activities and a topoisomerase activity in a site-specific DNA recombinase.
Molecular cell 1998;1(5):729-39.
1998: Lee J; Voziyanov Y; Pathania S; Jayaram M
Structural alterations and conformational dynamics in Holliday junctions induced by binding of a site-specific recombinase.
Molecular cell 1998;1(4):483-93.
1998: Namgoong S Y; Kim K; Saxena P; Yang J Y; Jayaram M; Giedroc D P; Harshey R M
Mutational analysis of domain II beta of bacteriophage Mu transposase: domains II alpha and II beta belong to different catalytic complementation groups.
Journal of molecular biology 1998;275(2):221-32.
1997: Ahn Y T; Wu X L; Biswal S; Velmurugan S; Volkert F C; Jayaram M
The 2microm-plasmid-encoded Rep1 and Rep2 proteins interact with each other and colocalize to the Saccharomyces cerevisiae nucleus.
Journal of bacteriology 1997;179(23):7497-506.
1997: Lee J; Tonozuka T; Jayaram M
Mechanism of active site exclusion in a site-specific recombinase: role of the DNA substrate in conferring half-of-the-sites activity.
Genes & development 1997;11(22):3061-71.
1997: Lee J; Jayaram M
A tetramer of the Flp recombinase silences the trimers within it during resolution of a Holliday junction substrate.
Genes & development 1997;11(18):2438-47.
1997: Saxena P; Whang I; Voziyanov Y; Harkey C; Argos P; Jayaram M; Dandekar T
Probing Flp: a new approach to analyze the structure of a DNA recognizing protein by combining the genetic algorithm, mutagenesis and non-canonical DNA target sites.
Biochimica et biophysica acta 1997;1340(2):187-204.
1997: Jayaram M
The cis-trans paradox of integrase.
Science (New York, N.Y.) 1997;276(5309):49-51.
1997: Knudsen B R; Dahlstrøm K; Westergaard O; Jayaram M
The yeast site-specific recombinase Flp mediates alcoholysis and hydrolysis of the strand cleavage product: mimicking the strand-joining reaction with non-DNA nucleophiles.
Journal of molecular biology 1997;266(1):93-107.
1996: Yang J Y; Jayaram M; Harshey R M
Positional information within the Mu transposase tetramer: catalytic contributions of individual monomers.
Cell 1996;85(3):447-55.
1996: Lee J; Whang I; Jayaram M
Assembly and orientation of Flp recombinase active sites on two-, three- and four-armed DNA substrates: implications for a recombination mechanism.
Journal of molecular biology 1996;257(3):532-549.
1996: Voziyanov Y; Lee J; Whang I; Lee J; Jayaram M
Analyses of the first chemical step in Flp site-specific recombination: Synapsis may not be a pre-requisite for strand cleavage.
Journal of molecular biology 1996;256(4):720-35.
1995: Yang J Y; Jayaram M; Harshey R M
Enhancer-independent variants of phage Mu transposase: enhancer-specific stimulation of catalytic activity by a partner transposase.
Genes & development 1995;9(20):2545-55.
1995: Lee J; Jayaram M
Functional roles of individual recombinase monomers in strand breakage and strand union during site-specific DNA recombination.
The Journal of biological chemistry 1995;270(39):23203-11.
1995: Lee J; Lee J; Jayaram M
Junction mobility and resolution of Holliday structures by Flp site-specific recombinase. Testing partner compatibility during recombination.
The Journal of biological chemistry 1995;270(32):19086-92.
1995: Yang J Y; Kim K; Jayaram M; Harshey R M
A domain sharing model for active site assembly within the Mu A tetramer during transposition: the enhancer may specify domain contributions.
The EMBO journal 1995;14(10):2374-84.
1995: Lee J; Jayaram M
Role of partner homology in DNA recombination. Complementary base pairing orients the 5'-hydroxyl for strand joining during Flp site-specific recombination.
The Journal of biological chemistry 1995;270(8):4042-52.
1995: Kim K; Namgoong S Y; Jayaram M; Harshey R M
Step-arrest mutants of phage Mu transposase. Implications in DNA-protein assembly, Mu end cleavage, and strand transfer.
The Journal of biological chemistry 1995;270(3):1472-9.
1994: Lee J; Whang I; Lee J; Jayaram M
Directed protein replacement in recombination full sites reveals trans-horizontal DNA cleavage by Flp recombinase.
The EMBO journal 1994;13(22):5346-54.
1994: Whang I; Lee J; Jayaram M
Active-site assembly and mode of DNA cleavage by Flp recombinase during full-site recombination.
Molecular and cellular biology 1994;14(11):7492-8.
1994: Namgoong S Y; Jayaram M; Kim K; Harshey R M
DNA-protein cooperativity in the assembly and stabilization of mu strand transfer complex. Relevance of DNA phasing and att site cleavage.
Journal of molecular biology 1994;238(4):514-27.
1994: Yang S H; Jayaram M
Generality of the shared active site among yeast family site-specific recombinases. The R site-specific recombinase follows the Flp paradigm [corrected]
The Journal of biological chemistry 1994;269(17):12789-96.
1994: Jayaram M
Phosphoryl transfer in Flp recombination: a template for strand transfer mechanisms.
Trends in biochemical sciences 1994;19(2):78-82.
1994: Saxena P; Whang I; Lee J; Lee J; Voziyanov Y; Mendoza V; Jayaram M
Role of tyrosine phosphorylation-dephosphorylation in copy number control of the yeast plasmid 2 micron circle.
Cellular & molecular biology research 1994;40(3):215-22.
1993: Lee J; Jayaram M
Mechanism of site-specific recombination. Logic of assembling recombinase catalytic site from fractional active sites.
The Journal of biological chemistry 1993;268(23):17564-70.
1993: Chen J W; Yang S H; Jayaram M
Tests for the fractional active-site model in Flp site-specific recombination. Assembly of a functional recombination complex in half-site and full-site strand transfer.
The Journal of biological chemistry 1993;268(19):14417-25.
1993: Serre M C; Zheng L; Jayaram M
DNA splicing by an active site mutant of Flp recombinase. Possible catalytic cooperativity between the inactive protein and its DNA substrate.
The Journal of biological chemistry 1993;268(1):455-63.
1992: Lee J; Serre M C; Yang S H; Whang I; Araki H; Oshima Y; Jayaram M
Functional analysis of Box II mutations in yeast site-specific recombinases Flp and R. Significance of amino acid conservation within the Int family and the yeast sub-family.
Journal of molecular biology 1992;228(4):1091-103.
1992: Chen J W; Evans B R; Yang S H; Araki H; Oshima Y; Jayaram M
Functional analysis of box I mutations in yeast site-specific recombinases Flp and R: pairwise complementation with recombinase variants lacking the active-site tyrosine.
Molecular and cellular biology 1992;12(9):3757-65.
1992: Chen J W; Evans B; Rosenfeldt H; Jayaram M
Bending-incompetent variants of Flp recombinase mediate strand transfer in half-site recombinations: role of DNA bending in recombination.
Gene 1992;119(1):37-48.
1992: Serre M C; Jayaram M
Half-site strand transfer by step-arrest mutants of yeast site-specific recombinase Flp.
Journal of molecular biology 1992;225(3):643-9.
1992: Serre M C; Evans B R; Araki H; Oshima Y; Jayaram M
Half-site recombinations mediated by yeast site-specific recombinases Flp and R.
Journal of molecular biology 1992;225(3):621-42.
1992: Chen J W; Lee J; Jayaram M
DNA cleavage in trans by the active site tyrosine during Flp recombination: switching protein partners before exchanging strands.
Cell 1992;69(4):647-58.
1992: Araki H; Nakanishi N; Evans B R; Matsuzaki H; Jayaram M; Oshima Y
Site-specific recombinase, R, encoded by yeast plasmid pSR1.
Journal of molecular biology 1992;225(1):25-37.
1991: Chen J W; Evans B R; Yang S H; Teplow D B; Jayaram M
Domain of a yeast site-specific recombinase (Flp) that recognizes its target site.
Proceedings of the National Academy of Sciences of the United States of America 1991;88(14):5944-8.
1991: Kuo C F; Zou A H; Jayaram M; Getzoff E; Harshey R
DNA-protein complexes during attachment-site synapsis in Mu DNA transposition.
The EMBO journal 1991;10(6):1585-91.
1991: Chen J W; Evans B R; Zheng L; Jayaram M
Tyr60 variants of Flp recombinase generate conformationally altered protein-DNA complexes. Differential activity in full-site and half-site recombinations.
Journal of molecular biology 1991;218(1):107-18.
1990: Evans B R; Chen J W; Parsons R L; Bauer T K; Teplow D B; Jayaram M
Identification of the active site tyrosine of Flp recombinase. Possible relevance of its location to the mechanism of recombination.
The Journal of biological chemistry 1990;265(30):18504-10.
1990: Parsons R L; Evans B R; Zheng L; Jayaram M
Functional analysis of Arg-308 mutants of Flp recombinase. Possible role of Arg-308 in coupling substrate binding to catalysis.
The Journal of biological chemistry 1990;265(8):4527-33.
1988: Choi H T; Revuelta J L; Sadhu C; Jayaram M
Structural organization of the TRP1 gene of Phycomyces blakesleeanus: implications for evolutionary gene fusion in fungi.
Gene 1988;71(1):85-95.
1988: Jayaram M; Crain K L; Parsons R L; Harshey R M
Holliday junctions in FLP recombination: resolution by step-arrest mutants of FLP protein.
Proceedings of the National Academy of Sciences of the United States of America 1988;85(21):7902-6.
1988: Parsons R L; Prasad P V; Harshey R M; Jayaram M
Step-arrest mutants of FLP recombinase: implications for the catalytic mechanism of DNA recombination.
Molecular and cellular biology 1988;8(8):3303-10.
1987: Revuelta J L; Jayaram M
Phycomyces blakesleeanus TRP1 gene: organization and functional complementation in Escherichia coli and Saccharomyces cerevisiae.
Molecular and cellular biology 1987;7(8):2664-70.
1986: Revuelta J L; Jayaram M
Transformation of Phycomyces blakesleeanus to G-418 resistance by an autonomously replicating plasmid.
Proceedings of the National Academy of Sciences of the United States of America 1986;83(19):7344-7.
1986: Jayaram M; Sumida S; Young L J
Inducible expression of REP1 causes inducible expression of the 2 micron circle stability system.
Current genetics 1986;11(2):85-91.

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