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Michael Kalafatis

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Chemicals & Drugs

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Publications

TOP 3
Melissa A Barhoover; Michael Kalafatis
Cleavage at both Arg306 and Arg506 is required and sufficient for timely and efficient inactivation of factor Va by activated protein C.
Jamila Hirbawi; John L Vaughn; Michael A Bukys; Hans L Vos; Michael Kalafatis
Contribution of amino acid region 659-663 of Factor Va heavy chain to the activity of factor Xa within prothrombinase .
Melissa A Barhoover; Tivadar Orban; Michael A Bukys; Michael Kalafatis
Cooperative regulation of the activity of factor Xa within prothrombinase by discrete amino acid regions from factor Va heavy chain.

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All Publications

2011: Melissa A Barhoover; Michael Kalafatis
Cleavage at both Arg306 and Arg506 is required and sufficient for timely and efficient inactivation of factor Va by activated protein C.
Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis 2011;22(4):317-24.
2010: Jamila Hirbawi; John L Vaughn; Michael A Bukys; Hans L Vos; Michael Kalafatis
Contribution of amino acid region 659-663 of Factor Va heavy chain to the activity of factor Xa within prothrombinase .
Biochemistry 2010;49(39):8520-34.
2008: Melissa A Barhoover; Tivadar Orban; Michael A Bukys; Michael Kalafatis
Cooperative regulation of the activity of factor Xa within prothrombinase by discrete amino acid regions from factor Va heavy chain.
Biochemistry 2008;47(48):12835-43.
2008: Jamila Hirbawi; Michael A Bukys; Melissa A Barhoover; Evrim Erdogan; Michael Kalafatis
Role of the acidic hirudin-like COOH-terminal amino acid region of factor Va heavy chain in the enhanced function of prothrombinase.
Biochemistry 2008;47(30):7963-74.
2008: Melissa A Barhoover; Tivadar Orban; Daniel O Beck; Michael A Bukys; Michael Kalafatis
Contribution of amino acid region 334-335 from factor Va heavy chain to the catalytic efficiency of prothrombinase.
Biochemistry 2008;47(26):6840-50.
2008: Michael A Bukys; Tivadar Orban; Paul Y Kim; Michael E Nesheim; Michael Kalafatis
The interaction of fragment 1 of prothrombin with the membrane surface is a prerequisite for optimum expression of factor Va cofactor activity within prothrombinase.
Thrombosis and haemostasis 2008;99(3):511-22.
2008: E Erdogan; Michael A Bukys; Michael Kalafatis
The contribution of amino acid residues 1508-1515 of factor V to light chain generation.
Journal of thrombosis and haemostasis : JTH 2008;6(1):118-24.
2007: Evrim Erdogan; Michael A Bukys; Thomas Orfeo; Kenneth G Mann; Michael Kalafatis
Identification of an inactivating cleavage site for alpha-thrombin on the heavy chain of factor Va.
Thrombosis and haemostasis 2007;98(5):998-1006.
2006: Michael A Bukys; Paul Y Kim; Michael E Nesheim; Michael Kalafatis
A control switch for prothrombinase: characterization of a hirudin-like pentapeptide from the COOH terminus of factor Va heavy chain that regulates the rate and pathway for prothrombin activation.
The Journal of biological chemistry 2006;281(51):39194-204.
2006: Saulius Butenas; Thomas Orfeo; Michael Kalafatis; Kenneth G Mann
Peptidomimetic inhibitors for activated protein C: implications for hemophilia management.
Journal of thrombosis and haemostasis : JTH 2006;4(11):2411-6.
2006: Michael A Bukys; Tivadar Orban; Paul Y Kim; Daniel O Beck; Michael E Nesheim; Michael Kalafatis
The structural integrity of anion binding exosite I of thrombin is required and sufficient for timely cleavage and activation of factor V and factor VIII.
The Journal of biological chemistry 2006;281(27):18569-80.
2005: Tivadar Orban; Michael Kalafatis; Valentin Gogonea
Completed three-dimensional model of human coagulation factor va. Molecular dynamics simulations and structural analyses.
Biochemistry 2005;44(39):13082-90.
2005: Michael A Bukys; Melissa A Blum; Paul Y Kim; Nicole Brufatto; Michael E Nesheim; Michael Kalafatis
Incorporation of factor Va into prothrombinase is required for coordinated cleavage of prothrombin by factor Xa.
The Journal of biological chemistry 2005;280(29):27393-401.
2005: Weston R Gould; Paolo Simioni; Jay R Silveira; Daniela Tormene; Michael Kalafatis; Paula B Tracy
Megakaryocytes endocytose and subsequently modify human factor V in vivo to form the entire pool of a unique platelet-derived cofactor.
Journal of thrombosis and haemostasis : JTH 2005;3(3):450-6.
2005: Michael Kalafatis
Coagulation factor V: a plethora of anticoagulant molecules.
Current opinion in hematology 2005;12(2):141-8.
2004: Daniel O Beck; Michael A Bukys; Shanjukumar Lisam; Katalin A Szabo; Michael Kalafatis
The contribution of amino acid region ASP695-TYR698 of factor V to procofactor activation and factor Va function.
The Journal of biological chemistry 2004;279(4):3084-95.
2003: Michael Kalafatis; Daniel O Beck; Kenneth G Mann
Structural requirements for expression of factor Va activity.
The Journal of biological chemistry 2003;278(35):33550-61.
2003: Shanjukumar Lisam; Michael A Bukys; Daniel O Beck; Michael Kalafatis
Amino acids Glu323, Tyr324, Glu330, and Val331 of factor Va heavy chain are essential for expression of cofactor activity.
The Journal of biological chemistry 2003;278(30):28335-45.
2003: Kenneth G Mann; Michael Kalafatis
Factor V: a combination of Dr Jekyll and Mr Hyde.
Blood 2003;101(1):20-30.
2002: Michael Kalafatis; Daniel O Beck
Identification of a binding site for blood coagulation factor Xa on the heavy chain of factor Va. Amino acid residues 323-331 of factor V represent an interactive site for activated factor X.
Biochemistry 2002;41(42):12715-28.
2002: Shanjukumar Lisam; Michael Kalafatis
Sequencing of full-length cDNA encoding the alpha and beta subunits of human casein kinase II from human platelets and megakaryocytic cells. Expression of the casein kinase IIalpha intronless gene in a megakaryocytic cell line.
Biochemistry 2002;41(28):8935-40.
2002: Michael Kalafatis; Paolo Simioni; Daniela Tormene; Daniel O Beck; Sonia Luni; Antonio Girolami
Isolation and characterization of an antifactor V antibody causing activated protein C resistance from a patient with severe thrombotic manifestations.
Blood 2002;99(11):3985-92.
2002: K Hung; Xiyuan Sun; H Ding; Michael Kalafatis; Paolo Simioni; Baochuan Guo
A matrix-assisted laser desorption/ionization time-of-flight based method for screening the 1691G --> A mutation in the factor V gene.
Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis 2002;13(2):117-22.
2002: Jay R Silveira; Michael Kalafatis; Paula B Tracy
Carbohydrate moieties on the procofactor factor V, but not the derived cofactor factor Va, regulate its inactivation by activated protein C.
Biochemistry 2002;41(5):1672-80.
2001: Paolo Simioni; Michael Kalafatis; Daniela Tormene; Sonia Luni; Patrizia Zerbinati; Luisa Barzon; G Palù; Antonio Girolami
Abnormal propeptide processing resulting in the presence of two abnormal species of protein C in plasma: characterization of the dysfunctional protein C Padua3 (protein C(R-1L/propeptide)).
Thrombosis and haemostasis 2001;86(4):1017-22.
2001: Michael Kalafatis; Kenneth G Mann
The role of the membrane in the inactivation of factor va by plasmin. Amino acid region 307-348 of factor V plays a critical role in factor Va cofactor function.
The Journal of biological chemistry 2001;276(21):18614-23.
2001: Paolo Simioni; Fabrizio Vianello; Michael Kalafatis; Luisa Barzon; Saverio Ladogana; Paolo Paolucci; M Carotenuto; Federico Dal Bello; Giorgio Palù; Antonio Girolami
A dysfunctional factor X (factor X San Giovanni Rotondo) present at homozygous and double heterozygous level: identification of a novel microdeletion (delC556) and missense mutation (Lys(408)-->Asn) in the factor X gene. A study of an Italian family.
Thrombosis research 2001;101(4):219-30.
2001: Michael Kalafatis; Kenneth G Mann
Factor V: Dr. Jeckyll and Mr. Hyde.
Advances in experimental medicine and biology 2001;489():31-43.
2000: Elisabetta Castoldi; Paolo Simioni; Michael Kalafatis; Barbara Lunghi; Daniela Tormene; Domenico Girelli; A Girolami; francesco bernardi
Combinations of 4 mutations (FV R506Q, FV H1299R, FV Y1702C, PT 20210G/A) affecting the prothrombinase complex in a thrombophilic family.
Blood 2000;96(4):1443-8.
1999: Matthew F Hockin; Kevin Cawthern; Michael Kalafatis; Kenneth G Mann
A model describing the inactivation of factor Va by APC: bond cleavage, fragment dissociation, and product inhibition.
Biochemistry 1999;38(21):6918-34.
1999: Michael Kalafatis; F Bernardi; Paolo Simioni; Barbara Lunghi; A Girolami; Kenneth G Mann
Phenotype and genotype expression in pseudohomozygous factor VLEIDEN : the need for phenotype analysis.
Arteriosclerosis, thrombosis, and vascular biology 1999;19(2):336-42.
1998: Paolo Simioni; Michael Kalafatis; D Manfrin; Daniela Tormene; Antonio Girolami
Factor V variants, activated protein C resistance and venous thromboembolism.
Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis 1998;9(7):661-2.
1998: Elisabetta Castoldi; Michael Kalafatis; Barbara Lunghi; Paolo Simioni; P A Ioannou; M Petio; A Girolami; K G Mann; francesco bernardi
Molecular bases of pseudo-homozygous APC resistance: the compound heterozygosity for FV R506Q and a FV null mutation results in the exclusive presence of FV Leiden molecules in plasma.
Thrombosis and haemostasis 1998;80(3):403-6.
1998: Rodney M Camire; Michael Kalafatis; Paula B Tracy
Proteolysis of factor V by cathepsin G and elastase indicates that cleavage at Arg1545 optimizes cofactor function by facilitating factor Xa binding.
Biochemistry 1998;37(34):11896-906.
1998: George L Long; D Lu; R L Xie; Michael Kalafatis
Human protein S cleavage and inactivation by coagulation factor Xa.
The Journal of biological chemistry 1998;273(19):11521-6.
1998: Rodney M Camire; Michael Kalafatis; Paolo Simioni; A Girolami; Paula B Tracy
Platelet-derived factor Va/Va Leiden cofactor activities are sustained on the surface of activated platelets despite the presence of activated protein C.
Blood 1998;91(8):2818-29.
1998: Michael Kalafatis
Identification and partial characterization of factor Va heavy chain kinase from human platelets.
The Journal of biological chemistry 1998;273(14):8459-66.
1997: Matthew F Hockin; Michael Kalafatis; MA Shatos; Kenneth G Mann
Protein C activation and factor Va inactivation on human umbilical vein endothelial cells.
Arteriosclerosis, thrombosis, and vascular biology 1997;17(11):2765-75.
1997: C van 't Veer; NJ Golden; Michael Kalafatis; Paolo Simioni; R M Bertina; Kenneth G Mann
An in vitro analysis of the combination of hemophilia A and factor V(LEIDEN).
Blood 1997;90(8):3067-72.
1997: JO Egan; Michael Kalafatis; Kenneth G Mann
The effect of Arg306-->Ala and Arg506-->Gln substitutions in the inactivation of recombinant human factor Va by activated protein C and protein S.
Protein science : a publication of the Protein Society 1997;6(9):2016-27.
1997: C van 't Veer; Michael Kalafatis; R M Bertina; Paolo Simioni; Kenneth G Mann
Increased tissue factor-initiated prothrombin activation as a result of the Arg506 --> Gln mutation in factor VLEIDEN.
The Journal of biological chemistry 1997;272(33):20721-9.
1997: Kenneth G Mann; Matthew F Hockin; Kelly J Begin; Michael Kalafatis
Activated protein C cleavage of factor Va leads to dissociation of the A2 domain.
The Journal of biological chemistry 1997;272(33):20678-83.
1997: Michael Kalafatis; Kenneth G Mann
Factor VLeiden and thrombophilia.
Arteriosclerosis, thrombosis, and vascular biology 1997;17(4):620-7.
1997: C van 't Veer; NJ Golden; Michael Kalafatis; Kenneth G Mann
Inhibitory mechanism of the protein C pathway on tissue factor-induced thrombin generation. Synergistic effect in combination with tissue factor pathway inhibitor.
The Journal of biological chemistry 1997;272(12):7983-94.
1997: Saulius Butenas; Michael Kalafatis; Kenneth G Mann
Analysis of tissue plasminogen activator specificity using peptidyl fluorogenic substrates.
Biochemistry 1997;36(8):2123-31.
1997: Michael Kalafatis; JO Egan; C van 't Veer; Kevin Cawthern; Kenneth G Mann
The regulation of clotting factors.
Critical reviews in eukaryotic gene expression 1997;7(3):241-80.
1996: Laszlo Bajzar; Michael Kalafatis; Paolo Simioni; Paula B Tracy
An antifibrinolytic mechanism describing the prothrombotic effect associated with factor VLeiden.
The Journal of biological chemistry 1996;271(38):22949-52.
1996: D Lu; Michael Kalafatis; Kenneth G Mann; George L Long
Comparison of activated protein C/protein S-mediated inactivation of human factor VIII and factor V.
Blood 1996;87(11):4708-17.
1996: Michael Kalafatis; P E Haley; D Lu; R M Bertina; George L Long; Kenneth G Mann
Proteolytic events that regulate factor V activity in whole plasma from normal and activated protein C (APC)-resistant individuals during clotting: an insight into the APC-resistance assay.
Blood 1996;87(11):4695-707.
1996: Michael Kalafatis; J O Egan; C van't Veer; Kenneth G Mann
Regulation and regulatory role of gamma-carboxyglutamic acid containing clotting factors.
Critical reviews in eukaryotic gene expression 1996;6(1):87-101.
1995: Michael Kalafatis; D Lu; R M Bertina; George L Long; Kenneth G Mann
Biochemical prototype for familial thrombosis. A study combining a functional protein C mutation and factor V Leiden.
Arteriosclerosis, thrombosis, and vascular biology 1995;15(12):2181-7.
1995: Rodney M Camire; Michael Kalafatis; M Cushman; Russell P Tracy; Kenneth G Mann; Paula B Tracy
The mechanism of inactivation of human platelet factor Va from normal and activated protein C-resistant individuals.
The Journal of biological chemistry 1995;270(35):20794-800.
1995: Michael Kalafatis; R M Bertina; M D Rand; Kenneth G Mann
Characterization of the molecular defect in factor VR506Q.
The Journal of biological chemistry 1995;270(8):4053-7.
1994: Michael Kalafatis; M D Rand; Kenneth G Mann
The mechanism of inactivation of human factor V and human factor Va by activated protein C.
The Journal of biological chemistry 1994;269(50):31869-80.
1994: Michael Kalafatis; N A Swords; M D Rand; Kenneth G Mann
Membrane-dependent reactions in blood coagulation: role of the vitamin K-dependent enzyme complexes.
Biochimica et biophysica acta 1994;1227(3):113-29.
1994: J Xue; Michael Kalafatis; J R Silveira; C Kung; Kenneth G Mann
Determination of the disulfide bridges in factor Va heavy chain.
Biochemistry 1994;33(44):13109-16.
1994: Jeffrey H Lawson; Michael Kalafatis; S Stram; Kenneth G Mann
A model for the tissue factor pathway to thrombin. I. An empirical study.
The Journal of biological chemistry 1994;269(37):23357-66.
1994: D Lu; Michael Kalafatis; Kenneth G Mann; George L Long
Loss of membrane-dependent factor Va cleavage: a mechanistic interpretation of the pathology of protein CVermont.
Blood 1994;84(3):687-90.
1994: Michael Kalafatis; J Xue; C M Lawler; Kenneth G Mann
Contribution of the heavy and light chains of factor Va to the interaction with factor Xa.
Biochemistry 1994;33(21):6538-45.
1994: M D Rand; Michael Kalafatis; Kenneth G Mann
Platelet coagulation factor Va: the major secretory platelet phosphoprotein.
Blood 1994;83(8):2180-90.
1994: Saulius Butenas; Jeffrey H Lawson; Michael Kalafatis; Kenneth G Mann
Cooperative interaction of divalent metal ions, substrate, and tissue factor with factor VIIa.
Biochemistry 1994;33(11):3449-56.
1994: Michael Kalafatis; M D Rand; Kenneth G Mann
Factor Va-membrane interaction is mediated by two regions located on the light chain of the cofactor.
Biochemistry 1994;33(2):486-93.
1993: Michael Kalafatis; Kenneth G Mann
Role of the membrane in the inactivation of factor Va by activated protein C.
The Journal of biological chemistry 1993;268(36):27246-57.
1993: J Xue; Michael Kalafatis; Kenneth G Mann
Determination of the disulfide bridges in factor Va light chain.
Biochemistry 1993;32(22):5917-23.
1993: Michael Kalafatis; M D Rand; R J Jenny; Y H Ehrlich; Kenneth G Mann
Phosphorylation of factor Va and factor VIIIa by activated platelets.
Blood 1993;81(3):704-19.
1993: Michael Kalafatis; Senthil Kumar Krishnaswamy; M D Rand; Kenneth G Mann
Factor V.
Methods in enzymology 1993;222():224-36.
1990: Michael Kalafatis; R J Jenny; Kenneth G Mann
Identification and characterization of a phospholipid-binding site of bovine factor Va.
The Journal of biological chemistry 1990;265(35):21580-9.