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Shigenori Kanaya

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Tri-Nhan Nguyen; Dong-Ju You; Eiko Kanaya; Yuichi Koga; Shigenori Kanaya
Crystal structure of metagenome-derived LC9-RNase H1 with atypical DEDN active site motif.
Tri-Nhan Nguyen; Dong-Ju You; Eiko Kanaya; Yuichi Koga; Hiroyuki Matsumoto; Shigenori Kanaya
Crystal structure of metagenome-derived LC11-RNase H1 in complex with RNA/DNA hybrid.
Takashi Tadokoro; Kazufumi Takano; Hirotaka Kazama; Yuichi Koga; Shigenori Kanaya
Investigating the structural dependence of protein stabilization by amino Acid substitution.

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2013: Tri-Nhan Nguyen; Dong-Ju You; Eiko Kanaya; Yuichi Koga; Shigenori Kanaya
Crystal structure of metagenome-derived LC9-RNase H1 with atypical DEDN active site motif.
FEBS letters 2013;587(9):1418-23.
2013: Tri-Nhan Nguyen; Dong-Ju You; Eiko Kanaya; Yuichi Koga; Hiroyuki Matsumoto; Shigenori Kanaya
Crystal structure of metagenome-derived LC11-RNase H1 in complex with RNA/DNA hybrid.
Journal of structural biology 2013;182(2):144-54.
2013: Takashi Tadokoro; Kazufumi Takano; Hirotaka Kazama; Yuichi Koga; Shigenori Kanaya
Investigating the structural dependence of protein stabilization by amino Acid substitution.
Biochemistry 2013;52(16):2839-47.
2013: Muhammad Tayyab; Clement Angkawidjaja; Shigenori Kanaya; Naeem Rashid; Muhammad Akhtar
Crystallization and X-ray structure determination of a thermoalkalophilic lipase from Geobacillus SBS-4S.
Acta crystallographica. Section F, Structural biology and crystallization communications 2013;69(Pt 4):355-7.
2013: Ryo Uehara; Dong-Ju You; Yuichi Koga; Yasunori Ueda; Shigenori Kanaya
Accelerated maturation of Tk-subtilisin by a Leu→Pro mutation at the C-terminus of the propeptide, which reduces the binding of the propeptide to Tk-subtilisin.
The FEBS journal 2013;280(4):994-1006.
2012: Jun Okada; Kazufumi Takano; Yuichi Koga; Shigenori Kanaya
Slow unfolding pathway of hyperthermophilic Tk-RNase H2 examined by pulse proteolysis using the stable protease Tk-subtilisin.
Biochemistry 2012;51(45):9178-91.
2012: Ryo Uehara; Yuichi Koga; Kazufumi Takano; Shun-Ichi Tanaka; Shigenori Kanaya
Requirement of insertion sequence IS1 for thermal adaptation of Pro-Tk-subtilisin from hyperthermophilic archaeon.
Extremophiles : life under extreme conditions 2012;16(6):841-51.
2012: Maria Cheng; Clement Angkawidjaja; Yuichi Koga; Shigenori Kanaya
Requirement of lid2 for interfacial activation of a family I.3 lipase with unique two lid structures.
The FEBS journal 2012;279(19):3727-37.
2012: Clement Angkawidjaja; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Structure and stability of a thermostable carboxylesterase from the thermoacidophilic archaeon Sulfolobus tokodaii.
The FEBS journal 2012;279(17):3071-84.
2012: Nujarin Jongruja; Eiko Kanaya; Yuichi Koga; Dong-Ju You; Clement Angkawidjaja; Shigenori Kanaya
Structure and characterization of RNase H3 from Aquifex aeolicus.
The FEBS journal 2012;279(15):2737-53.
2012: Joong-Jae Kim; Yuichi Koga; Soon-Wo Kwon; Kazufumi Takano; Hang-Yeon Weon; Peter F Dunfield; Eiko Kanaya; Shigenori Kanaya
Flavobacterium compostarboris sp. nov., isolated from leaf-and-branch compost, and emended descriptions of Flavobacterium hercynium, Flavobacterium resistens and Flavobacterium johnsoniae.
International journal of systematic and evolutionary microbiology 2012;62(Pt 8):2018-24.
2012: Ryo Uehara; Yuichi Koga; Kazufumi Takano; Yuki Takeuchi; Shun-ichi Tanaka; Shigenori Kanaya
Requirement of Ca(2+) ions for the hyperthermostability of Tk-subtilisin from Thermococcus kodakarensis.
Biochemistry 2012;51(26):5369-78.
2012: Tri-Nhan Nguyen; Kazufumi Takano; Clement Angkawidjaja; Eiko Kanaya; Yuichi Koga; Shigenori Kanaya
Activity, stability, and structure of metagenome-derived LC11-RNase H1, a homolog of Sulfolobus tokodaii RNase H1.
Protein science : a publication of the Protein Society 2012;21(4):553-61.
2012: Cahyo Budiman; Yuichi Koga; Takashi Tadokoro; Clement Angkawidjaja; Shigenori Kanaya
Role of polar and nonpolar residues at the active site for PPIase activity of FKBP22 from Shewanella sp. SIB1.
The FEBS journal 2012;279(6):976-86.
2012: Ken Okada; Kazufumi Takano; Clement Angkawidjaja; Yuichi Koga; Shigenori Kanaya
Characteristic features of kynurenine aminotransferase allosterically regulated by (alpha)-ketoglutarate in cooperation with kynurenine.
PloS one 2012;7(7):e40307.
2011: Cahyo Budiman; Yuichi Koga; Hideki Motoike; Kazufumi Takano; Clement Angkawidjaja; Shigenori Kanaya
Crystal structure of N-domain of FKBP22 from Shewanella sp. SIB1: dimer dissociation by disruption of Val-Leu knot.
Protein science : a publication of the Protein Society 2011;20(10):1755-64.
2011: Seiko Miyashita; Takashi Tadokoro; Kazufumi Takano; Dong-Ju You; Clement Angkawidjaja; Yuichi Koga; Shigenori Kanaya
Identification of the substrate binding site in the N-terminal TBP-like domain of RNase H3.
FEBS letters 2011;585(14):2313-7.
2011: Joong-Jae Kim; Yuichi Koga; Hyo Jung Lee; Kazufumi Takano; Che Ok Jeon; Hyun Mi Jin; Eiko Kanaya; Shigenori Kanaya
Flavobacterium banpakuense sp. nov., isolated from leaf-and-branch compost.
International journal of systematic and evolutionary microbiology 2011;61(Pt 7):1595-600.
2011: Nitat Sinsereekul; Tita Foophow; Mai Yamanouchi; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
An alternative mature form of subtilisin homologue, Tk-SP, from Thermococcus kodakaraensis identified in the presence of Ca2+.
The FEBS journal 2011;278(11):1901-11.
2011: K Kuwahara; Clement Angkawidjaja; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Importance of an extreme C-terminal motif of a family I.3 lipase for stability.
Protein engineering, design & selection : PEDS 2011;24(5):411-8.
2011: Muhammad Tayyab; Naeem Rashid; Clement Angkawidjaja; Shigenori Kanaya; Muhammad Akhtar
Highly active metallocarboxypeptidase from newly isolated Geobacillus strain SBS-4S: cloning and characterization.
Journal of bioscience and bioengineering 2011;111(3):259-65.
2011: Shun-ichi Tanaka; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Inhibition of chymotrypsin- and subtilisin-like serine proteases with Tk-serpin from hyperthermophilic archaeon Thermococcus kodakaraensis.
Biochimica et biophysica acta 2011;1814(2):299-307.
2011: Cahyo Budiman; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
FK506-Binding Protein 22 from a Psychrophilic Bacterium, a Cold Shock-Inducible Peptidyl Prolyl Isomerase with the Ability to Assist in Protein Folding.
International journal of molecular sciences 2011;12(8):5261-84.
2011: Kazufumi Takano; Tomohiro Okamoto; Jun Okada; Shun-ichi Tanaka; Clement Angkawidjaja; Yuichi Koga; Shigenori Kanaya
Stabilization by fusion to the C-terminus of hyperthermophile Sulfolobus tokodaii RNase HI: a possibility of protein stabilization tag.
PloS one 2011;6(1):e16226.
2011: Aya Sato; Sonoko Yokotani; Takashi Tadokoro; Shun-ichi Tanaka; Clement Angkawidjaja; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Crystal structure of stable protein CutA1 from psychrotrophic bacterium Shewanella sp. SIB1.
Journal of synchrotron radiation 2011;18(1):6-10.
2010: Nujarin Jongruja; Dong-Ju You; Eiko Kanaya; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+-dependent activity.
The FEBS journal 2010;277(21):4474-89.
2010: Masaki Tanaka; Hyongi Chon; Clement Angkawidjaja; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Protein core adaptability: crystal structures of the cavity-filling variants of Escherichia coli RNase HI.
Protein and peptide letters 2010;17(9):1163-9.
2010: E Kanaya; T Sakabe; N T Nguyen; S Koikeda; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Cloning of the RNase H genes from a metagenomic DNA library: identification of a new type 1 RNase H without a typical active-site motif.
Journal of applied microbiology 2010;109(3):974-83.
2010: Tita Foophow; Shun-ichi Tanaka; Clement Angkawidjaja; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Crystal structure of a subtilisin homologue, Tk-SP, from Thermococcus kodakaraensis: requirement of a C-terminal beta-jelly roll domain for hyperstability.
Journal of molecular biology 2010;400(4):865-77.
2010: Clement Angkawidjaja; Hiroyoshi Matsumura; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
X-ray crystallographic and MD simulation studies on the mechanism of interfacial activation of a family I.3 lipase with two lids.
Journal of molecular biology 2010;400(1):82-95.
2010: T Foophow; S Tanaka; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Subtilisin-like serine protease from hyperthermophilic archaeon Thermococcus kodakaraensis with N- and C-terminal propeptides.
Protein engineering, design & selection : PEDS 2010;23(5):347-55.
2010: Jun Okada; Tomohiro Okamoto; Atsushi Mukaiyama; Takashi Tadokoro; Dong-Ju You; Hyongi Chon; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Evolution and thermodynamics of the slow unfolding of hyperstable monomeric proteins.
BMC evolutionary biology 2010;10():207.
2010: Tomohisa Kato; Asuka Miyanaga; Shigenori Kanaya; Masaaki Morikawa
Gene cloning and characterization of an aldehyde dehydrogenase from long-chain alkane-degrading Geobacillus thermoleovorans B23.
Extremophiles : life under extreme conditions 2010;14(1):33-9.
2009: Shun-ichi Tanaka; Hiroyoshi Matsumura; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Identification of the interactions critical for propeptide-catalyzed folding of Tk-subtilisin.
Journal of molecular biology 2009;394(2):306-19.
2009: Cahyo Budiman; Keisuke Bando; Clement Angkawidjaja; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Engineering of monomeric FK506-binding protein 22 with peptidyl prolyl cis-trans isomerase. Importance of a V-shaped dimeric structure for binding to protein substrate.
The FEBS journal 2009;276(15):4091-101.
2009: Takashi Tadokoro; Shigenori Kanaya
Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes.
The FEBS journal 2009;276(6):1482-93.
2009: Tomohisa Kato; Asuka Miyanaga; Shigenori Kanaya; Masaaki Morikawa
Alkane inducible proteins in Geobacillus thermoleovorans B23.
BMC microbiology 2009;9():60.
2009: Muhammad S Rohman; Takashi Tadokoro; Clement Angkawidjaja; Yumi Abe; Hiroyoshi Matsumura; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses.
The FEBS journal 2009;276(2):603-13.
2008: Shun-ichi Tanaka; Yuki Takeuchi; Hiroyoshi Matsumura; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Crystal structure of Tk-subtilisin folded without propeptide: requirement of propeptide for acceleration of folding.
FEBS letters 2008;582(28):3875-8.
2008: Muhammad S Rohman; Yuichi Koga; Kazufumi Takano; Hyongi Chon; Robert J Crouch; Shigenori Kanaya
Effect of the disease-causing mutations identified in human ribonuclease (RNase) H2 on the activities and stabilities of yeast RNase H2 and archaeal RNase HII.
The FEBS journal 2008;275(19):4836-49.
2008: Takashi Tadokoro; Kyoko Matsushita; Yumi Abe; Muhammad Saifur Rohman; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Remarkable stabilization of a psychrotrophic RNase HI by a combination of thermostabilizing mutations identified by the suppressor mutation method.
Biochemistry 2008;47(31):8040-7.
2008: Yuichi Koga; Ryota Katsumi; Dong-Ju You; Hiroyoshi Matsumura; Kazufumi Takano; Shigenori Kanaya
Crystal structure of highly thermostable glycerol kinase from a hyperthermophilic archaeon in a dimeric form.
The FEBS journal 2008;275(10):2632-43.
2008: Hongju Dong; Atsushi Mukaiyama; Takashi Tadokoro; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Hydrophobic effect on the stability and folding of a hyperthermophilic protein.
Journal of molecular biology 2008;378(1):264-72.
2008: Atsushi Mukaiyama; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Osmolyte effect on the stability and folding of a hyperthermophilic protein.
Proteins 2008;71(1):110-8.
2007: Marian A Pulido; Shun-ichi Tanaka; Chutima Sringiew; Dong-Ju You; Hiroyoshi Matsumura; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Requirement of left-handed glycine residue for high stability of the Tk-subtilisin propeptide as revealed by mutational and crystallographic analyses.
Journal of molecular biology 2007;374(5):1359-73.
2007: Clement Angkawidjaja; Dong-Ju You; Hiroyoshi Matsumura; Katsumasa Kuwahara; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation.
FEBS letters 2007;581(26):5060-4.
2007: Dong-Ju You; Hyongi Chon; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Crystal structure of type 1 ribonuclease H from hyperthermophilic archaeon Sulfolobus tokodaii: role of arginine 118 and C-terminal anchoring.
Biochemistry 2007;46(41):11494-503.
2007: Dong-Ju You; Satoshi Fukuchi; Ken Nishikawa; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Protein thermostabilization requires a fine-tuned placement of surface-charged residues.
Journal of biochemistry 2007;142(4):507-16.
2007: Shun-Ichi Tanaka; Hiroyoshi Matsumura; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Four new crystal structures of Tk-subtilisin in unautoprocessed, autoprocessed and mature forms: insight into structural changes during maturation.
Journal of molecular biology 2007;372(4):1055-69.
2007: Kazufumi Takano; Yoshiaki Katagiri; Atsushi Mukaiyama; Hyongi Chon; Hiroyoshi Matsumura; Yuichi Koga; Shigenori Kanaya
Conformational contagion in a protein: structural properties of a chameleon sequence.
Proteins 2007;68(3):617-25.
2007: Takashi Tadokoro; Hyongi Chon; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Identification of the gene encoding a type 1 RNase H with an N-terminal double-stranded RNA binding domain from a psychrotrophic bacterium.
The FEBS journal 2007;274(14):3715-27.
2007: Takashi Tadokoro; Dong-Ju You; Yumi Abe; Hyongi Chon; Hiroyoshi Matsumura; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Structural, thermodynamic, and mutational analyses of a psychrotrophic RNase HI.
Biochemistry 2007;46(25):7460-8.
2007: Shun-ichi Tanaka; Kenji Saito; Hyongi Chon; Hiroyoshi Matsumura; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Crystal structure of unautoprocessed precursor of subtilisin from a hyperthermophilic archaeon: evidence for Ca2+-induced folding.
The Journal of biological chemistry 2007;282(11):8246-55.
2007: M A Pulido; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Directed evolution of Tk-subtilisin from a hyperthermophilic archaeon: identification of a single amino acid substitution responsible for low-temperature adaptation.
Protein engineering, design & selection : PEDS 2007;20(3):143-53.
2007: Clement Angkawidjaja; Dong-Ju You; Hiroyoshi Matsumura; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Extracellular overproduction and preliminary crystallographic analysis of a family I.3 lipase.
Acta crystallographica. Section F, Structural biology and crystallization communications 2007;63(Pt 3):187-9.
2007: Ryota Katsumi; Yuichi Koga; Dong-Ju You; Hiroyoshi Matsumura; Kazufumi Takano; Shigenori Kanaya
Crystallization and preliminary X-ray diffraction study of glycerol kinase from the hyperthermophilic archaeon Thermococcus kodakaraensis.
Acta crystallographica. Section F, Structural biology and crystallization communications 2007;63(Pt 2):126-9.
2007: Takehiko Wada; Yoshiki Maeda; Nobuya Sawa; Hirofumi Sato; Hyongi Chon; Shigenori Kanaya; Yoshihisa Inoue
Synthesis of peptide ribonucleic acid (PRNA)-DNA chimera and interaction with DNA and RNA.
Nucleic acids symposium series (2004) 2007;(51):21-2.
2007: Shin-ichi Hirano; Masaaki Morikawa; Kazufumi Takano; Tadayuki Imanaka; Shigenori Kanaya
Gentisate 1,2-dioxygenase from Xanthobacter polyaromaticivorans 127W.
Bioscience, biotechnology, and biochemistry 2007;71(1):192-9.
2006: Clement Angkawidjaja; Shigenori Kanaya
Family I.3 lipase: bacterial lipases secreted by the type I secretion system.
Cellular and molecular life sciences : CMLS 2006;63(23):2804-17.
2006: Atsushi Mukaiyama; Mitsuru Haruki; Motonori Ota; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
A hyperthermophilic protein acquires function at the cost of stability.
Biochemistry 2006;45(42):12673-9.
2006: Masafumi Kashii; Ryo Fujisawa; Hiroaki Adachi; Yusuke Mori; Takatomo Sasaki; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya; Hiroyoshi Matsumura; Tsuyoshi Inoue; Satoshi Murakami; Kazuomi Sugamoto; Hideki Yoshikawa
Application of femtosecond laser ablation for detaching grown protein crystals from glass capillary tube.
Journal of bioscience and bioengineering 2006;102(4):372-4.
2006: Shun-ichi Tanaka; Kenji Saito; Hyongi Chon; Hiroyoshi Matsumura; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Crystallization and preliminary X-ray diffraction study of an active-site mutant of pro-Tk-subtilisin from a hyperthermophilic archaeon.
Acta crystallographica. Section F, Structural biology and crystallization communications 2006;62(Pt 9):902-5.
2006: Masaaki Morikawa; Shinji Kagihiro; Mitsuru Haruki; Kazufumi Takano; Steven S. Branda; Roberto Kolter; Shigenori Kanaya
Biofilm formation by a Bacillus subtilis strain that produces gamma-polyglutamate.
Microbiology (Reading, England) 2006;152(Pt 9):2801-7.
2006: Jiraporn Thaniyavarn; Aree Chongchin; Nopparat Wanitsuksombut; Suthep Thaniyavarn; Pairoh Pinphanichakarn; Natthanant Leepipatpiboon; Masaaki Morikawa; Shigenori Kanaya
Biosurfactant production by Pseudomonas aeruginosa A41 using palm oil as carbon source.
The Journal of general and applied microbiology 2006;52(4):215-22.
2006: Dong-Ju You; Hyongi Chon; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Crystallization and preliminary crystallographic analysis of type 1 RNase H from the hyperthermophilic archaeon Sulfolobus tokodaii 7.
Acta crystallographica. Section F, Structural biology and crystallization communications 2006;62(Pt 8):781-4.
2006: Clement Angkawidjaja; K Kuwahara; Kenji Omori; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Extracellular secretion of Escherichia coli alkaline phosphatase with a C-terminal tag by type I secretion system: purification and biochemical characterization.
Protein engineering, design & selection : PEDS 2006;19(7):337-43.
2006: Marian Pulido; Kenji Saito; Shun-Ichi Tanaka; Yuichi Koga; Masaaki Morikawa; Kazufumi Takano; Shigenori Kanaya
Ca2+-dependent maturation of subtilisin from a hyperthermophilic archaeon, Thermococcus kodakaraensis: the propeptide is a potent inhibitor of the mature domain but is not required for its folding.
Applied and environmental microbiology 2006;72(6):4154-62.
2006: Hyongi Chon; Takashi Tadokoro; Naoto Ohtani; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Identification of RNase HII from psychrotrophic bacterium, Shewanella sp. SIB1 as a high-activity type RNase H.
The FEBS journal 2006;273(10):2264-75.
2006: Joel C Cornista; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Amyloidogenecity and pitrilysin sensitivity of a lysine-free derivative of amyloid beta-peptide cleaved from a recombinant fusion protein.
Journal of biotechnology 2006;122(2):186-97.
2006: Hyongi Chon; Hiroyoshi Matsumura; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Crystal structure and structure-based mutational analyses of RNase HIII from Bacillus stearothermophilus: a new type 2 RNase H with TBP-like substrate-binding domain at the N terminus.
Journal of molecular biology 2006;356(1):165-78.
2006: Shin-Ichi Hirano; Mitsuru Haruki; Kazufumi Takano; Tadayuki Imanaka; Masaaki Morikawa; Shigenori Kanaya
Gene cloning and in vivo characterization of a dibenzothiophene dioxygenase from Xanthobacter polyaromaticivorans.
Applied microbiology and biotechnology 2006;69(6):672-81.
2006: Kazufumi Takano; Shuji Endo; Atsushi Mukaiyama; Hyongi Chon; Hiroyoshi Matsumura; Yuichi Koga; Shigenori Kanaya
Structure of amyloid beta fragments in aqueous environments.
The FEBS journal 2006;273(1):150-8.
2006: Takahiko Yamamoto; Tomoki Matsuda; Tsuyoshi Inoue; Hiroyoshi Matsumura; Masaaki Morikawa; Shigenori Kanaya; Yasushi Kai
Crystal structure of TBP-interacting protein (Tk-TIP26) and implications for its inhibition mechanism of the interaction between TBP and TATA-DNA.
Protein science : a publication of the Protein Society 2006;15(1):152-61.
2005: Hyongi Chon; Hiroyoshi Matsumura; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Crystal structure of a human kynurenine aminotransferase II homologue from Pyrococcus horikoshii OT3 at 2.20 A resolution.
Proteins 2005;61(3):685-8.
2005: Niran Roongsawang; Siew Ping Lim; Kenji Washio; Kazufumi Takano; Shigenori Kanaya; Masaaki Morikawa
Phylogenetic analysis of condensation domains in the nonribosomal peptide synthetases.
FEMS microbiology letters 2005;252(1):143-51.
2005: Yutaka Suzuki; Ohnmar Ye Win; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Binding analysis of a psychrotrophic FKBP22 to a folding intermediate of protein using surface plasmon resonance.
FEBS letters 2005;579(25):5781-4.
2005: Clement Angkawidjaja; Aditya Paul; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Importance of a repetitive nine-residue sequence motif for intracellular stability and functional structure of a family I.3 lipase.
FEBS letters 2005;579(21):4707-12.
2005: Hyongi Chon; Hiroyoshi Matsumura; Shoko Shimizu; Nao Maeda; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Overproduction and preliminary crystallographic study of a human kynurenine aminotransferase II homologue from Pyrococcus horikoshii OT3.
Acta crystallographica. Section F, Structural biology and crystallization communications 2005;61(Pt 3):319-22.
2005: Hyongi Chon; Hiroyoshi Matsumura; Yuichi Koga; Kazufumi Takano; Shigenori Kanaya
Crystallization and preliminary X-ray diffraction study of thermostable RNase HIII from Bacillus stearothermophilus.
Acta crystallographica. Section F, Structural biology and crystallization communications 2005;61(Pt 3):293-5.
2005: Yasuo Tsunaka; Kazufumi Takano; Hiroyoshi Matsumura; Yuriko Yamagata; Shigenori Kanaya
Identification of single Mn(2+) binding sites required for activation of the mutant proteins of E.coli RNase HI at Glu48 and/or Asp134 by X-ray crystallography.
Journal of molecular biology 2005;345(5):1171-83.
2005: Yutaka Suzuki; Yoichi Mizutani; Tadao Tsuji; Naoto Ohtani; Kazufumi Takano; Mitsuru Haruki; Masaaki Morikawa; Shigenori Kanaya
Gene cloning, overproduction, and characterization of thermolabile alkaline phosphatase from a psychrotrophic bacterium.
Bioscience, biotechnology, and biochemistry 2005;69(2):364-73.
2005: Yutaka Suzuki; Kazufumi Takano; Shigenori Kanaya
Stabilities and activities of the N- and C-domains of FKBP22 from a psychrotrophic bacterium overproduced in Escherichia coli.
The FEBS journal 2005;272(3):632-42.
2005: Namio Kunihiro; Mitsuru Haruki; Kazufumi Takano; Masaaki Morikawa; Shigenori Kanaya
Isolation and characterization of Rhodococcus sp. strains TMP2 and T12 that degrade 2,6,10,14-tetramethylpentadecane (pristane) at moderately low temperatures.
Journal of biotechnology 2005;115(2):129-36.
2004: Atsushi Mukaiyama; Kazufumi Takano; Mitsuru Haruki; Masaaki Morikawa; Shigenori Kanaya
Kinetically robust monomeric protein from a hyperthermophile.
Biochemistry 2004;43(43):13859-66.
2004: Hyongi Chon; Rikita Nakano; Naoto Ohtani; Mitsuru Haruki; Kazufumi Takano; Masaaki Morikawa; Shigenori Kanaya
Gene cloning and biochemical characterizations of thermostable ribonuclease HIII from Bacillus stearothermophilus.
Bioscience, biotechnology, and biochemistry 2004;68(10):2138-47.
2004: Joel Cornista; Satoshi Ikeuchi; Mitsuru Haruki; Atsuko Kohara; Kazufumi Takano; Masaaki Morikawa; Shigenori Kanaya
Cleavage of various peptides with pitrilysin from Escherichia coli: kinetic analyses using beta-endorphin and its derivatives.
Bioscience, biotechnology, and biochemistry 2004;68(10):2128-37.
2004: Kazufumi Takano; Minoru Saito; Masaaki Morikawa; Shigenori Kanaya
Mutational and structural-based analyses of the osmolyte effect on protein stability.
Journal of biochemistry 2004;135(6):701-8.
2004: Yutaka Suzuki; Mitsuru Haruki; Kazufumi Takano; Masaaki Morikawa; Shigenori Kanaya
Possible involvement of an FKBP family member protein from a psychrotrophic bacterium Shewanella sp. SIB1 in cold-adaptation.
European journal of biochemistry / FEBS 2004;271(7):1372-81.
2004: Shin-ichi Hirano; Fumiya Kitauchi; Mitsuru Haruki; Tadayuki Imanaka; Masaaki Morikawa; Shigenori Kanaya
Isolation and characterization of Xanthobacter polyaromaticivorans sp. nov. 127W that degrades polycyclic and heterocyclic aromatic compounds under extremely low oxygen conditions.
Bioscience, biotechnology, and biochemistry 2004;68(3):557-64.
2003: Niran Roongsawang; Ken ichi Hase; Mitsuru Haruki; Tadayuki Imanaka; Masaaki Morikawa; Shigenori Kanaya
Cloning and characterization of the gene cluster encoding arthrofactin synthetase from Pseudomonas sp. MIS38.
Chemistry & biology 2003;10(9):869-80.
2003: Jiraporn Thaniyavarn; Niran Roongsawang; Takayuki Kameyama; Mitsuru Haruki; Tadayuki Imanaka; Masaaki Morikawa; Shigenori Kanaya
Production and characterization of biosurfactants from Bacillus licheniformis F2.2.
Bioscience, biotechnology, and biochemistry 2003;67(6):1239-44.
2003: Kazuhiko Yamasaki; Tomoko Yamasaki; Shigenori Kanaya; Motohisa Oobatake
Acid-induced denaturation of Escherichia coli ribonuclease HI analyzed by CD and NMR spectroscopies.
Biopolymers 2003;69(2):176-88.
2003: Yasuo Tsunaka; Mitsuru Haruki; Masaaki Morikawa; Motohisa Oobatake; Shigenori Kanaya
Dispensability of glutamic acid 48 and aspartic acid 134 for Mn2+-dependent activity of Escherichia coli ribonuclease HI.
Biochemistry 2003;42(11):3366-74.
2003: Takahiko Yamamoto; Tomoki Matsuda; Nami Sakamoto; Hiroyoshi Matsumura; Tsuyoshi Inoue; Masaaki Morikawa; Shigenori Kanaya; Yasushi Kai
Crystallization and preliminary X-ray analysis of TBP-interacting protein from the hyperthermophilic archaeon Thermococcus kodakaraensis strain KOD1.
Acta crystallographica. Section D, Biological crystallography 2003;59(Pt 2):372-4.
2003: Hiroaki Adachi; Kazufumi Takano; Masaaki Morikawa; Shigenori Kanaya; Masashi Yoshimura; Yusuke Mori; Takatomo Sasaki
Application of a two-liquid system to sitting-drop vapour-diffusion protein crystallization.
Acta crystallographica. Section D, Biological crystallography 2003;59(Pt 1):194-6.
2002: Niran Roongsawang; Jiraporn Thaniyavarn; Suthep Thaniyavarn; Takayuki Kameyama; Mitsuru Haruki; Tadayuki Imanaka; Masaaki Morikawa; Shigenori Kanaya
Isolation and characterization of a halotolerant Bacillus subtilis BBK-1 which produces three kinds of lipopeptides: bacillomycin L, plipastatin, and surfactin.
Extremophiles : life under extreme conditions 2002;6(6):499-506.
2002: Mitsuru Haruki; Yasuo Tsunaka; Masaaki Morikawa; Shigenori Kanaya
Cleavage of a DNA-RNA-DNA/DNA chimeric substrate containing a single ribonucleotide at the DNA-RNA junction with prokaryotic RNases HII.
FEBS letters 2002;531(2):204-8.
2002: Hyongi Chon; Yasuo Tsunaka; Mitsuru Haruki; Masaaki Morikawa; Shigenori Kanaya
Site-specific cleavage of MS2 RNA by a thermostable DNA-linked RNase H.
Protein engineering 2002;15(8):683-8.
2002: Ayumu Muroya; Rikita Nakano; Naoto Ohtani; Mitsuru Haruki; Masaaki Morikawa; Shigenori Kanaya
Importance of an N-terminal extension in ribonuclease HII from Bacillus stearothermophilus for substrate binding.
Journal of bioscience and bioengineering 2002;93(2):170-5.
2002: Hyun-Ju Kwon; Mitsuru Haruki; Masaaki Morikawa; Kenji Omori; Shigenori Kanaya
Role of repetitive nine-residue sequence motifs in secretion, enzymatic activity, and protein conformation of a family I.3 lipase.
Journal of bioscience and bioengineering 2002;93(2):157-64.
2001: Naoto Ohtani; Mitsuru Haruki; Masaaki Morikawa; Shigenori Kanaya
Heat labile ribonuclease HI from a psychrotrophic bacterium: gene cloning, characterization and site-directed mutagenesis.
Protein engineering 2001;14(12):975-82.
2001: K Amada; H J Kwon; Mitsuru Haruki; Masaaki Morikawa; Shigenori Kanaya
Ca(2+)-induced folding of a family I.3 lipase with repetitive Ca(2+) binding motifs at the C-terminus.
FEBS letters 2001;509(1):17-21.
2001: T Kato; Mitsuru Haruki; Tadayuki Imanaka; Masaaki Morikawa; Shigenori Kanaya
Isolation and characterization of psychotrophic bacteria from oil-reservoir water and oil sands.
Applied microbiology and biotechnology 2001;55(6):794-800.
2001: Tomoki Matsuda; M Fujikawa; Mitsuru Haruki; XF Tang; Satoshi Ezaki; Tadayuki Imanaka; Masaaki Morikawa; Shigenori Kanaya
Interaction of TIP26 from a hyperthermophilic archaeon with TFB/TBP/DNA ternary complex.
Extremophiles : life under extreme conditions 2001;5(3):177-82.
2001: Y Kannan; Yuichi Koga; Y Inoue; Mitsuru Haruki; Masahiro Takagi; Tadayuki Imanaka; Masaaki Morikawa; Shigenori Kanaya
Active subtilisin-like protease from a hyperthermophilic archaeon in a form with a putative prosequence.
Applied and environmental microbiology 2001;67(6):2445-52.
2001: Yasuo Tsunaka; Mitsuru Haruki; Masaaki Morikawa; Shigenori Kanaya
Strong nucleic acid binding to the Escherichia coli RNase HI mutant with two arginine residues at the active site.
Biochimica et biophysica acta 2001;1547(1):135-42.
2001: Ayumu Muroya; D Tsuchiya; M Ishikawa; Mitsuru Haruki; Masaaki Morikawa; Shigenori Kanaya; Kosuke Morikawa
Catalytic center of an archaeal type 2 ribonuclease H as revealed by X-ray crystallographic and mutational analyses.
Protein science : a publication of the Protein Society 2001;10(4):707-14.
2001: Yuichi Koga; Mitsuru Haruki; Masaaki Morikawa; Shigenori Kanaya
Stabilities of chimeras of hyperthermophilic and mesophilic glycerol kinases constructed by DNA shuffling.
Journal of bioscience and bioengineering 2001;91(6):551-6.
2001: Tomoaki Kato; A Miyanaga; Mitsuru Haruki; Tadayuki Imanaka; Masaaki Morikawa; Shigenori Kanaya
Gene Cloning of an alcohol dehydrogenase from thermophilic alkane-degrading Bacillus thermoleovorans B23.
Journal of bioscience and bioengineering 2001;91(1):100-2.
2001: Tomoaki Kato; Mitsuru Haruki; Tadayuki Imanaka; Masaaki Morikawa; Shigenori Kanaya
Isolation and characterization of long-chain-alkane degrading Bacillus thermoleovorans from deep subterranean petroleum reservoirs.
Journal of bioscience and bioengineering 2001;91(1):64-70.
2001: Shigenori Kanaya
Prokaryotic type 2 RNases H.
Methods in enzymology 2001;341():377-94.
2000: Mitsuru Haruki; T Nogawa; N Hirano; Hyongi Chon; Yasuo Tsunaka; Masaaki Morikawa; Shigenori Kanaya
Efficient cleavage of RNA at high temperatures by a thermostable DNA-linked ribonuclease H.
Protein engineering 2000;13(12):881-6.
2000: Mitsuru Haruki; Yasuo Tsunaka; Masaaki Morikawa; Shigenori Iwai; Shigenori Kanaya
Catalysis by Escherichia coli ribonuclease HI is facilitated by a phosphate group of the substrate.
Biochemistry 2000;39(45):13939-44.
2000: N Hirano; Mitsuru Haruki; Masaaki Morikawa; Shigenori Kanaya
Enhancement of the enzymatic activity of ribonuclease HI from Thermus thermophilus HB8 with a suppressor mutation method.
Biochemistry 2000;39(43):13285-94.
2000: H J Kwon; K Amada; Mitsuru Haruki; Masaaki Morikawa; Shigenori Kanaya; K Hyun-Ju
Identification of the histidine and aspartic acid residues essential for enzymatic activity of a family I.3 lipase by site-directed mutagenesis.
FEBS letters 2000;483(2-3):139-42.
2000: K Amada; Mitsuru Haruki; Tadayuki Imanaka; Masaaki Morikawa; Shigenori Kanaya
Overproduction in Escherichia coli, purification and characterization of a family I.3 lipase from Pseudomonas sp. MIS38.
Biochimica et biophysica acta 2000;1478(2):201-10.
2000: Naoto Ohtani; Mitsuru Haruki; Ayumu Muroya; Masaaki Morikawa; Shigenori Kanaya
Characterization of ribonuclease HII from Escherichia coli overproduced in a soluble form.
Journal of biochemistry 2000;127(5):895-9.
1999: S Mizuguchi; K Amada; Mitsuru Haruki; Tadayuki Imanaka; Masaaki Morikawa; Shigenori Kanaya
Identification of the gene encoding esterase, a homolog of hormone-sensitive lipase, from an oil-degrading bacterium, strain HD-1.
Journal of biochemistry 1999;126(4):731-7.
1999: Tomoki Matsuda; Masaaki Morikawa; Mitsuru Haruki; H Higashibata; Tadayuki Imanaka; Shigenori Kanaya
Isolation of TBP-interacting protein (TIP) from a hyperthermophilic archaeon that inhibits the binding of TBP to TATA-DNA.
FEBS letters 1999;457(1):38-42.
1999: Mitsuru Haruki; Y Oohashi; S Mizuguchi; Y Matsuo; Masaaki Morikawa; Shigenori Kanaya
Identification of catalytically essential residues in Escherichia coli esterase by site-directed mutagenesis.
FEBS letters 1999;454(3):262-6.
1999: M Izumi; Shinsuke Fujiwara; Masahiro Takagi; Shigenori Kanaya; Tadayuki Imanaka
Isolation and characterization of a second subunit of molecular chaperonin from Pyrococcus kodakaraensis KOD1: analysis of an ATPase-deficient mutant enzyme.
Applied and environmental microbiology 1999;65(4):1801-5.
1999: Naoto Ohtani; Mitsuru Haruki; Masaaki Morikawa; Robert J Crouch; Mitsuhiro Itaya; Shigenori Kanaya
Identification of the genes encoding Mn2+-dependent RNase HII and Mg2+-dependent RNase HIII from Bacillus subtilis: classification of RNases H into three families.
Biochemistry 1999;38(2):605-18.
1999: N Q Huy; S Jin; K Amada; Mitsuru Haruki; Huu Nguyen; D T Hang; D T Ha; Tadayuki Imanaka; Masaaki Morikawa; Shigenori Kanaya
Characterization of petroleum-degrading bacteria from oil-contaminated sites in Vietnam.
Journal of bioscience and bioengineering 1999;88(1):100-2.
1999: Naoto Ohtani; Mitsuru Haruki; Masaaki Morikawa; Shigenori Kanaya
Molecular diversities of RNases H.
Journal of bioscience and bioengineering 1999;88(1):12-9.
1999: N Okibe; K Amada; S Hirano; Mitsuru Haruki; Tadayuki Imanaka; Masaaki Morikawa; Shigenori Kanaya
Gene cloning and characterization of aldehyde dehydrogenase from a petroleum-degrading bacterium, strain HD-1.
Journal of bioscience and bioengineering 1999;88(1):7-11.
1998: Yuichi Koga; Masaaki Morikawa; Mitsuru Haruki; H Nakamura; Tadayuki Imanaka; Shigenori Kanaya
Thermostable glycerol kinase from a hyperthermophilic archaeon: gene cloning and characterization of the recombinant enzyme.
Protein engineering 1998;11(12):1219-27.
1998: Mitsuru Haruki; K Hayashi; T Kochi; Ayumu Muroya; Yuichi Koga; Masaaki Morikawa; Tadayuki Imanaka; Shigenori Kanaya
Gene cloning and characterization of recombinant RNase HII from a hyperthermophilic archaeon.
Journal of bacteriology 1998;180(23):6207-14.
1998: N Hirano; Mitsuru Haruki; Masaaki Morikawa; Shigenori Kanaya
Stabilization of ribonuclease HI from Thermus thermophilus HB8 by the spontaneous formation of an intramolecular disulfide bond.
Biochemistry 1998;37(36):12640-8.
1998: Shigenori Kanaya; T Koyanagi; E Kanaya
An esterase from Escherichia coli with a sequence similarity to hormone-sensitive lipase.
The Biochemical journal 1998;332 ( Pt 1)():75-80.
1998: K Yamasaki; A Akasako-Furukawa; Shigenori Kanaya
Structural stability and internal motions of Escherichia coli ribonuclease HI: 15N relaxation and hydrogen-deuterium exchange analyses.
Journal of molecular biology 1998;277(3):707-22.
1997: Mitsuru Haruki; E Noguchi; Shigenori Kanaya; Robert J Crouch
Kinetic and stoichiometric analysis for the binding of Escherichia coli ribonuclease HI to RNA-DNA hybrids using surface plasmon resonance.
The Journal of biological chemistry 1997;272(35):22015-22.
1997: A Akasako; Mitsuru Haruki; Motohisa Oobatake; Shigenori Kanaya
Conformational stabilities of Escherichia coli RNase HI variants with a series of amino acid substitutions at a cavity within the hydrophobic core.
The Journal of biological chemistry 1997;272(30):18686-93.
1997: N Rashid; Masaaki Morikawa; Keisuke Nagahisa; Shigenori Kanaya; Tadayuki Imanaka
Characterization of a RecA/RAD51 homologue from the hyperthermophilic archaeon Pyrococcus sp. KOD1.
Nucleic acids research 1997;25(4):719-26.
1996: Shigenori Kanaya; M Oobatake; Y Liu
Thermal stability of Escherichia coli ribonuclease HI and its active site mutants in the presence and absence of the Mg2+ ion. Proposal of a novel catalytic role for Glu48.
The Journal of biological chemistry 1996;271(51):32729-36.
1996: T Kashiwagi; Denis Jeanteur; Mitsuru Haruki; K Katayanagi; Shigenori Kanaya; K Morikawa
Proposal for new catalytic roles for two invariant residues in Escherichia coli ribonuclease HI.
Protein engineering 1996;9(10):857-67.
1996: Shinsuke Fujiwara; S G Lee; Mitsuru Haruki; Shigenori Kanaya; Masahiro Takagi; Tadayuki Imanaka
Unusual enzyme characteristics of aspartyl-tRNA synthetase from hyperthermophilic archaeon Pyrococcus sp. KOD1.
FEBS letters 1996;394(1):66-70.
1995: K Yamasaki; Kyoko Ogasahara; Katsuhide Yutani; M Oobatake; Shigenori Kanaya
Folding pathway of Escherichia coli ribonuclease HI: a circular dichroism, fluorescence, and NMR study.
Biochemistry 1995;34(51):16552-62.
1995: Shigenori Kanaya; T Uchida
Revised sequence of ribonuclease U2 in the substrate-binding region.
Journal of biochemistry 1995;118(4):681-2.
1995: E Kanaya; Shigenori Kanaya
Reconstitution of Escherichia coli RNase HI from the N-fragment with high helicity and the C-fragment with a disordered structure.
The Journal of biological chemistry 1995;270(34):19853-60.
1995: E Kanaya; Shigenori Kanaya
Kinetic analysis of Escherichia coli ribonuclease HI using oligomeric DNA/RNA substrates suggests an alternative mechanism for the interaction between the enzyme and the substrate.
European journal of biochemistry / FEBS 1995;231(3):557-62.
1995: A Akasako; Mitsuru Haruki; M Oobatake; Shigenori Kanaya
High resistance of Escherichia coli ribonuclease HI variant with quintuple thermostabilizing mutations to thermal denaturation, acid denaturation, and proteolytic degradation.
Biochemistry 1995;34(25):8115-22.
1995: K Yamasaki; M Saito; M Oobatake; Shigenori Kanaya
Characterization of the internal motions of Escherichia coli ribonuclease HI by a combination of 15N-NMR relaxation analysis and molecular dynamics simulation: examination of dynamic models.
Biochemistry 1995;34(20):6587-601.
1995: Shigenori Kanaya; M Ikehara
Functions and structures of ribonuclease H enzymes.
Sub-cellular biochemistry 1995;24():377-422.
1994: E Kanaya; Y Uchiyama; E Ohtsuka; Y Ueno; M Ikehara; Shigenori Kanaya
Kinetic analyses of DNA-linked ribonucleases H with different sizes of DNA.
FEBS letters 1994;354(2):227-31.
1994: Mitsuru Haruki; E Noguchi; A Akasako; M Oobatake; Mitsuhiro Itaya; Shigenori Kanaya
A novel strategy for stabilization of Escherichia coli ribonuclease HI involving a screen for an intragenic suppressor of carboxyl-terminal deletions.
The Journal of biological chemistry 1994;269(43):26904-11.
1994: Y Oda; T Yamazaki; K Nagayama; Shigenori Kanaya; Y Kuroda; H Nakamura
Individual ionization constants of all the carboxyl groups in ribonuclease HI from Escherichia coli determined by NMR.
Biochemistry 1994;33(17):5275-84.
1994: Mitsuru Haruki; E Noguchi; C Nakai; Y Y Liu; M Oobatake; Mitsuhiro Itaya; Shigenori Kanaya
Investigating the role of conserved residue Asp134 in Escherichia coli ribonuclease HI by site-directed random mutagenesis.
European journal of biochemistry / FEBS 1994;220(2):623-31.
1994: C Nakai; A Konishi; Y Komatsu; H Inoue; E Ohtsuka; Shigenori Kanaya
Sequence-specific cleavage of RNA by a hybrid ribonuclease H.
FEBS letters 1994;339(1-2):67-72.
1993: K Katayanagi; M Ishikawa; M Okumura; M Ariyoshi; Shigenori Kanaya; Y Kawano; M Suzuki; I Tanaka; K Morikawa
Crystal structures of ribonuclease HI active site mutants from Escherichia coli.
The Journal of biological chemistry 1993;268(29):22092-9.
1993: K Ishikawa; H Nakamura; K Morikawa; S Kimura; Shigenori Kanaya
Cooperative stabilization of Escherichia coli ribonuclease HI by insertion of Gly-80b and Gly-77-->Ala substitution.
Biochemistry 1993;32(28):7136-42.
1993: K Ishikawa; H Nakamura; K Morikawa; Shigenori Kanaya
Stabilization of Escherichia coli ribonuclease HI by cavity-filling mutations within a hydrophobic core.
Biochemistry 1993;32(24):6171-8.
1993: K Ishikawa; M Okumura; K Katayanagi; S Kimura; Shigenori Kanaya; H Nakamura; K Morikawa
Crystal structure of ribonuclease H from Thermus thermophilus HB8 refined at 2.8 A resolution.
Journal of molecular biology 1993;230(2):529-42.
1993: Shigenori Kanaya; M Oobatake; H Nakamura; M Ikehara
pH-dependent thermostabilization of Escherichia coli ribonuclease HI by histidine to alanine substitutions.
Journal of biotechnology 1993;28(1):117-36.
1993: Y Oda; M Yoshida; Shigenori Kanaya
Role of histidine 124 in the catalytic function of ribonuclease HI from Escherichia coli.
The Journal of biological chemistry 1993;268(1):88-92.
1993: M Okumura; K Ishikawa; Shigenori Kanaya; Mitsuhiro Itaya; K Morikawa
Crystallization and preliminary crystallographic analysis of ribonuclease H from Thermus thermophilus HB8.
Proteins 1993;15(1):108-11.
1993: K Ishikawa; S Kimura; Shigenori Kanaya; K Morikawa; H Nakamura
Structural study of mutants of Escherichia coli ribonuclease HI with enhanced thermostability.
Protein engineering 1993;6(1):85-91.
1992: S Kimura; Shigenori Kanaya; H Nakamura
Thermostabilization of Escherichia coli ribonuclease HI by replacing left-handed helical Lys95 with Gly or Asn.
The Journal of biological chemistry 1992;267(31):22014-7.
1992: S Kimura; H Nakamura; T Hashimoto; M Oobatake; Shigenori Kanaya
Stabilization of Escherichia coli ribonuclease HI by strategic replacement of amino acid residues with those from the thermophilic counterpart.
The Journal of biological chemistry 1992;267(30):21535-42.
1992: S Kimura; Y Oda; T Nakai; K Katayanagi; E Kitakuni; C Nakai; H Nakamura; M Ikehara; Shigenori Kanaya
Effect of cavity-modulating mutations on the stability of Escherichia coli ribonuclease HI.
European journal of biochemistry / FEBS 1992;206(2):337-43.
1992: Shigenori Kanaya; Mitsuhiro Itaya
Expression, purification, and characterization of a recombinant ribonuclease H from Thermus thermophilus HB8.
The Journal of biological chemistry 1992;267(14):10184-92.
1992: Shigenori Kanaya; C Nakai; A Konishi; H Inoue; E Ohtsuka; M Ikehara
A hybrid ribonuclease H. A novel RNA cleaving enzyme with sequence-specific recognition.
The Journal of biological chemistry 1992;267(12):8492-8.
1992: Y Oda; H Nakamura; T Yamazaki; K Nagayama; M Yoshida; Shigenori Kanaya; M Ikehara
1H NMR studies of deuterated ribonuclease HI selectively labeled with protonated amino acids.
Journal of biomolecular NMR 1992;2(2):137-47.
1992: K Katayanagi; M Miyagawa; M Matsushima; M Ishikawa; Shigenori Kanaya; H Nakamura; M Ikehara; T Matsuzaki; K Morikawa
Structural details of ribonuclease H from Escherichia coli as refined to an atomic resolution.
Journal of molecular biology 1992;223(4):1029-52.
1991: H Nakamura; Y Oda; S Iwai; H Inoue; E Ohtsuka; Shigenori Kanaya; S Kimura; C Katsuda; K Katayanagi; K Morikawa
How does RNase H recognize a DNA.RNA hybrid?
Proceedings of the National Academy of Sciences of the United States of America 1991;88(24):11535-9.
1991: Y Oda; H Nakamura; Shigenori Kanaya; M Ikehara
Binding of metal ions to E. coli RNase HI observed by 1H-15N heteronuclear 2D NMR.
Journal of biomolecular NMR 1991;1(3):247-55.
1991: Shigenori Kanaya; C Katsuda-Nakai; M Ikehara
Importance of the positive charge cluster in Escherichia coli ribonuclease HI for the effective binding of the substrate.
The Journal of biological chemistry 1991;266(18):11621-7.
1991: Shigenori Kanaya; K Katayanagi; K Morikawa; H Inoue; E Ohtsuka; M Ikehara
Effect of mutagenesis at each of five histidine residues on enzymatic activity and stability of ribonuclease H from Escherichia coli.
European journal of biochemistry / FEBS 1991;198(2):437-40.
1991: Shigenori Kanaya; C Katsuda; S Kimura; T Nakai; E Kitakuni; H Nakamura; K Katayanagi; K Morikawa; M Ikehara
Stabilization of Escherichia coli ribonuclease H by introduction of an artificial disulfide bond.
The Journal of biological chemistry 1991;266(10):6038-44.
1990: E Kanaya; Shigenori Kanaya; M Kikuchi
Introduction of a non-native disulfide bridge to human lysozyme by cysteine scanning mutagenesis.
Biochemical and biophysical research communications 1990;173(3):1194-9.
1990: Shigenori Kanaya; S Kimura; C Katsuda; M Ikehara
Role of cysteine residues in ribonuclease H from Escherichia coli. Site-directed mutagenesis and chemical modification.
The Biochemical journal 1990;271(1):59-66.
1990: K Katayanagi; M Miyagawa; M Matsushima; M Ishikawa; Shigenori Kanaya; M Ikehara; T Matsuzaki; K Morikawa
Three-dimensional structure of ribonuclease H from E. coli.
Nature 1990;347(6290):306-9.
1990: M Kikuchi; Y Taniyama; Shigenori Kanaya; T Takao; Y Shimonishi
Occurrence of S-(1,2-dicarboxyethyl)-cysteine at position 77 in mutant human lysozyme secreted by Saccharomyces cerevisiae.
European journal of biochemistry / FEBS 1990;187(2):315-20.
1989: Shigenori Kanaya; A Kohara; M Miyagawa; T Matsuzaki; K Morikawa; M Ikehara
Overproduction and preliminary crystallographic study of ribonuclease H from Escherichia coli.
The Journal of biological chemistry 1989;264(20):11546-9.