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Evan Kantrowitz

This is a preview profile on BiomedExperts - the first literature-based scientific social network. It brings the right researchers together and allows them to collaborate online. Collexis and Dell provide the BiomedExperts network of +1.5 Million pre-calculated profiles free of charge to researchers worldwide.

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Chemicals & Drugs

Living Beings

Escherichia coli

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Physiology

Procedures

X-Ray Crystallography

Phenomena

Catalysis

Genes & Molecular Sequences

Amino Acid Sequence

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Co-Publications
Name
15
Stec, Boguslaw
14
Tsuruta, Hiro
9
Williams, Mark
8
Vachette, Patrice
8
Stieglitz, Kimberly
8
Baker, Darren
7
Sakash, Jessica
6
Holtz, Kathleen
6
Lipscomb, William
6
Macol, Christine
6
Cardia, James
6
West, Jay
6
Xia, Jiarong
5
Eldo, Joby
4
Tauc, Patrick

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Publications

TOP 3
Heng Sabrina; Gryncel Kimberly R; Kantrowitz Evan R
A library of novel allosteric inhibitors against fructose 1,6-bisphosphatase.
West Jay M; Xia Jiarong; Tsuruta Hiro; Guo Wenyue; O'Day Elizabeth M; Kantrowitz Evan R
Time evolution of the quaternary structure of Escherichia coli aspartate transcarbamoylase upon reaction with the natural substrates and a slow, tight-binding inhibitor.
Wang Qin; Xia Jiarong; Guallar Victor; Krilov Goran; Kantrowitz Evan R
Mechanism of thermal decomposition of carbamoyl phosphate and its stabilization by aspartate and ornithine transcarbamoylases.

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All Publications

2009: Heng Sabrina; Gryncel Kimberly R; Kantrowitz Evan R
A library of novel allosteric inhibitors against fructose 1,6-bisphosphatase.
Bioorganic & medicinal chemistry 2009;17(11):3916-22.
2008: West Jay M; Xia Jiarong; Tsuruta Hiro; Guo Wenyue; O'Day Elizabeth M; Kantrowitz Evan R
Time evolution of the quaternary structure of Escherichia coli aspartate transcarbamoylase upon reaction with the natural substrates and a slow, tight-binding inhibitor.
Journal of molecular biology 2008;384(1):206-18.
2008: Wang Qin; Xia Jiarong; Guallar Victor; Krilov Goran; Kantrowitz Evan R
Mechanism of thermal decomposition of carbamoyl phosphate and its stabilization by aspartate and ornithine transcarbamoylases.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(44):16918-23.
2008: Rabinowitz Joshua D; Hsiao Jennifer J; Gryncel Kimberly R; Kantrowitz Evan R; Feng Xiao-Jiang; Li Genyuan; Rabitz Herschel
Dissecting enzyme regulation by multiple allosteric effectors: nucleotide regulation of aspartate transcarbamoylase.
Biochemistry 2008;47(21):5881-8.
2008: Cardia James P; Eldo Joby; Xia Jiarong; O'Day Elizabeth M; Tsuruta Hiro; Gryncel Kimberly R; Kantrowitz Evan R
Use of L-asparagine and N-phosphonacetyl-L-asparagine to investigate the linkage of catalysis and homotropic cooperativity in E. coli aspartate transcarbomoylase.
Proteins 2008;71(3):1088-96.
2007: Stec Boguslaw; Williams Mark K; Stieglitz Kimberly A; Kantrowitz Evan R
Comparison of two T-state structures of regulatory-chain mutants of Escherichia coli aspartate transcarbamoylase suggests that His20 and Asp19 modulate the response to heterotropic effectors.
Acta crystallographica. Section D, Biological crystallography 2007;63(Pt 12):1243-53.
2007: Wang Jie; Eldo Joby; Kantrowitz Evan R
Structural model of the R state of Escherichia coli aspartate transcarbamoylase with substrates bound.
Journal of molecular biology 2007;371(5):1261-73.
2007: Eldo Joby; Heng Sabrina; Kantrowitz Evan R
Design, synthesis, and bioactivity of novel inhibitors of E. coli aspartate transcarbamoylase.
Bioorganic & medicinal chemistry letters 2007;17(7):2086-90.
2007: Fetler Luc; Kantrowitz Evan R; Vachette Patrice
Direct observation in solution of a preexisting structural equilibrium for a mutant of the allosteric aspartate transcarbamoylase.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(2):495-500.
2006: Wang Jie; Kantrowitz Evan R
Trapping the tetrahedral intermediate in the alkaline phosphatase reaction by substitution of the active site serine with threonine.
Protein science : a publication of the Protein Society 2006;15(10):2395-401.
2006: Eldo Joby; Cardia James P; O'Day Elizabeth M; Xia Jiarong; Tsuruta Hiro; Kantrowitz Evan R
N-phosphonacetyl-L-isoasparagine a potent and specific inhibitor of Escherichia coli aspartate transcarbamoylase.
Journal of medicinal chemistry 2006;49(20):5932-8.
2006: Heng Sabrina; Stieglitz Kimberly A; Eldo Joby; Xia Jiarong; Cardia James P; Kantrowitz Evan R
T-state inhibitors of E. coli aspartate transcarbamoylase that prevent the allosteric transition.
Biochemistry 2006;45(33):10062-71.
2005: Stieglitz Kimberly A; Dusinberre Kelly J; Cardia James P; Tsuruta Hiro; Kantrowitz Evan R
Structure of the E.coli aspartate transcarbamoylase trapped in the middle of the catalytic cycle.
Journal of molecular biology 2005;352(2):478-86.
2005: Stec Boguslaw; Holtz Kathleen M; Wojciechowski Cheryl L; Kantrowitz Evan R
Structure of the wild-type TEM-1 beta-lactamase at 1.55 A and the mutant enzyme Ser70Ala at 2.1 A suggest the mode of noncovalent catalysis for the mutant enzyme.
Acta crystallographica. Section D, Biological crystallography 2005;61(Pt 8):1072-9.
2005: Wang Jie; Stieglitz Kimberly A; Cardia James P; Kantrowitz Evan R
Structural basis for ordered substrate binding and cooperativity in aspartate transcarbamoylase.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(25):8881-6.
2005: Wang Jie; Stieglitz Kimberly A; Kantrowitz Evan R
Metal specificity is correlated with two crucial active site residues in Escherichia coli alkaline phosphatase.
Biochemistry 2005;44(23):8378-86.
2005: Stieglitz Kimberly A; Pastra-Landis Styliani C; Xia Jiarong; Tsuruta Hiro; Kantrowitz Evan R
A single amino acid substitution in the active site of Escherichia coli aspartate transcarbamoylase prevents the allosteric transition.
Journal of molecular biology 2005;349(2):413-23.
2004: Stieglitz Kimberly; Stec Boguslaw; Baker Darren P; Kantrowitz Evan R
Monitoring the transition from the T to the R state in E.coli aspartate transcarbamoylase by X-ray crystallography: crystal structures of the E50A mutant enzyme in four distinct allosteric states.
Journal of molecular biology 2004;341(3):853-68.
2004: Alam Neelima; Stieglitz Kimberly A; Caban Michael D; Gourinath Samudrala; Tsuruta Hiro; Kantrowitz Evan R
240s loop interactions stabilize the T state of Escherichia coli aspartate transcarbamoylase.
The Journal of biological chemistry 2004;279(22):23302-10.
2004: West Jay M; Tsuruta Hiro; Kantrowitz Evan R
A fluorescent probe-labeled Escherichia coli aspartate transcarbamoylase that monitors the allosteric conformational state.
The Journal of biological chemistry 2004;279(2):945-51.
2003: West Jay M; Kantrowitz Evan R
Trapping specific quaternary states of the allosteric enzyme aspartate transcarbamoylase in silica matrix sol-gels.
Journal of the American Chemical Society 2003;125(33):9924-5.
2003: Wojciechowski Cheryl L; Kantrowitz Evan R
Glutamic acid residues as metal ligands in the active site of Escherichia coli alkaline phosphatase.
Biochimica et biophysica acta 2003;1649(1):68-73.
2003: Boulanger Robert R; Kantrowitz Evan R
Characterization of a monomeric Escherichia coli alkaline phosphatase formed upon a single amino acid substitution.
The Journal of biological chemistry 2003;278(26):23497-501.
2002: Chan Robin S; Sakash Jessica B; Macol Christine P; West Jay M; Tsuruta Hiro; Kantrowitz Evan R
The role of intersubunit interactions for the stabilization of the T state of Escherichia coli aspartate transcarbamoylase.
The Journal of biological chemistry 2002;277(51):49755-60.
2002: Wojciechowski Cheryl L; Kantrowitz Evan R
Altering of the metal specificity of Escherichia coli alkaline phosphatase.
The Journal of biological chemistry 2002;277(52):50476-81.
2002: West Jay M; Tsuruta Hiro; Kantrowitz Evan R
Stabilization of the R allosteric structure of Escherichia coli aspartate transcarbamoylase by disulfide bond formation.
The Journal of biological chemistry 2002;277(49):47300-4.
2002: Macol Christine P; Tsuruta Hiro; Kantrowitz Evan R
Importance of domain closure for the catalysis and regulation of Escherichia coli aspartate transcarbamoylase.
The Journal of biological chemistry 2002;277(30):26852-7.
2002: Fetler L; Tauc P; Baker D P; Macol C P; Kantrowitz E R; Vachette P
Replacement of Asp-162 by Ala prevents the cooperative transition by the substrates while enhancing the effect of the allosteric activator ATP on E. coli aspartate transcarbamoylase.
Protein science : a publication of the Protein Society 2002;11(5):1074-81.
2002: Wojciechowski Cheryl L; Cardia James P; Kantrowitz Evan R
Alkaline phosphatase from the hyperthermophilic bacterium T. maritima requires cobalt for activity.
Protein science : a publication of the Protein Society 2002;11(4):903-11.
2002: Kelley-Loughnane Nancy; Biolsi Susan A; Gibson Kate M; Lu Guqiang; Hehir Michael J; Phelan Paul; Kantrowitz Evan R
Purification, kinetic studies, and homology model of Escherichia coli fructose-1,6-bisphosphatase.
Biochimica et biophysica acta 2002;1594(1):6-16.
2001: Kelley-Loughnane N; Kantrowitz E R
Binding of AMP to two of the four subunits of pig kidney fructose-1,6-bisphosphatase induces the allosteric transition.
Proteins 2001;44(3):255-61.
2001: Kelley-Loughnane N; Kantrowitz E R
AMP inhibition of pig kidney fructose-1,6-bisphosphatase.
Biochimica et biophysica acta 2001;1548(1):66-71.
2001: Sakash J B; Williams M K; Tsuruta H; Kantrowitz E R
Domain bridging interactions. A necessary contribution to the function and structure of Escherichia coli aspartate transcarbamoylase.
The Journal of biological chemistry 2001;276(28):26441-7.
2001: McIninch J K; Kantrowitz E R
Use of silicate sol-gels to trap the R and T quaternary conformational states of pig kidney fructose-1,6-bisphosphatase.
Biochimica et biophysica acta 2001;1547(2):320-8.
2001: Macol C P; Tsuruta H; Stec B; Kantrowitz E R
Direct structural evidence for a concerted allosteric transition in Escherichia coli aspartate transcarbamoylase.
Nature structural biology 2001;8(5):423-6.
2000: Hehir M J; Murphy J E; Kantrowitz E R
Characterization of heterodimeric alkaline phosphatases from Escherichia coli: an investigation of intragenic complementation.
Journal of molecular biology 2000;304(4):645-56.
2000: Sakash J B; Kantrowitz E R
The contribution of individual interchain interactions to the stabilization of the T and R states of Escherichia coli aspartate transcarbamoylase.
The Journal of biological chemistry 2000;275(37):28701-7.
2000: Vitali J; Vorobyova T; Webster G; Kantrowitz E R
Crystallization and structure determination of the catalytic trimer of Methanococcus jannaschii aspartate transcarbamoylase.
Acta crystallographica. Section D, Biological crystallography 2000;56(Pt 8):1061-3.
2000: Holtz K M; Catrina I E; Hengge A C; Kantrowitz E R
Mutation of Arg-166 of alkaline phosphatase alters the thio effect but not the transition state for phosphoryl transfer. Implications for the interpretation of thio effects in reactions of phosphatases.
Biochemistry 2000;39(31):9451-8.
2000: Jin L; Stec B; Kantrowitz E R
A cis-proline to alanine mutant of E. coli aspartate transcarbamoylase: kinetic studies and three-dimensional crystal structures.
Biochemistry 2000;39(27):8058-66.
2000: Stec B; Holtz K M; Kantrowitz E R
A revised mechanism for the alkaline phosphatase reaction involving three metal ions.
Journal of molecular biology 2000;299(5):1303-11.
2000: Holtz K M; Stec B; Myers J K; Antonelli S M; Widlanski T S; Kantrowitz E R
Alternate modes of binding in two crystal structures of alkaline phosphatase-inhibitor complexes.
Protein science : a publication of the Protein Society 2000;9(5):907-15.
2000: Hack E S; Vorobyova T; Sakash J B; West J M; Macol C P; Hervé G; Williams M K; Kantrowitz E R
Characterization of the aspartate transcarbamoylase from Methanococcus jannaschii.
The Journal of biological chemistry 2000;275(21):15820-7.
2000: Sakash J B; Tsen A; Kantrowitz E R
The use of nucleotide analogs to evaluate the mechanism of the heterotropic response of Escherichia coli aspartate transcarbamoylase.
Protein science : a publication of the Protein Society 2000;9(1):53-63.
2000: Sakash J B; Chan R S; Tsuruta H; Kantrowitz E R
Three of the six possible intersubunit stabilizing interactions involving Glu-239 are sufficient for restoration of the homotropic and heterotropic properties of Escherichia coli aspartate transcarbamoylase.
The Journal of biological chemistry 2000;275(2):752-8.
1999: Jin L; Stec B; Lipscomb W N; Kantrowitz E R
Insights into the mechanisms of catalysis and heterotropic regulation of Escherichia coli aspartate transcarbamoylase based upon a structure of the enzyme complexed with the bisubstrate analogue N-phosphonacetyl-L-aspartate at 2.1 A.
Proteins 1999;37(4):729-42.
1999: Holtz K M; Kantrowitz E R
The mechanism of the alkaline phosphatase reaction: insights from NMR, crystallography and site-specific mutagenesis.
FEBS letters 1999;462(1-2):7-11.
1999: Sträter N; Sun L; Kantrowitz E R; Lipscomb W N
A bicarbonate ion as a general base in the mechanism of peptide hydrolysis by dizinc leucine aminopeptidase.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(20):11151-5.
1999: Macol C; Dutta M; Stec B; Tsuruta H; Kantrowitz E R
The 80s loop of the catalytic chain of Escherichia coli aspartate transcarbamoylase is critical for catalysis and homotropic cooperativity.
Protein science : a publication of the Protein Society 1999;8(6):1305-13.
1999: Martin D C; Pastra-Landis S C; Kantrowitz E R
Amino acid substitutions at the subunit interface of dimeric Escherichia coli alkaline phosphatase cause reduced structural stability.
Protein science : a publication of the Protein Society 1999;8(5):1152-9.
1999: Holtz K M; Stec B; Kantrowitz E R
A model of the transition state in the alkaline phosphatase reaction.
The Journal of biological chemistry 1999;274(13):8351-4.
1999: Sun L; Martin D C; Kantrowitz E R
Rate-determining step of Escherichia coli alkaline phosphatase altered by the removal of a positive charge at the active center.
Biochemistry 1999;38(9):2842-8.
1998: Williams M K; Kantrowitz E R
Threonine 82 in the regulatory chain is important for nucleotide affinity and for the allosteric stabilization of Escherichia coli aspartate transcarbamoylase.
Biochimica et biophysica acta 1998;1429(1):249-58.
1998: Williams M K; Stec B; Kantrowitz E R
A single mutation in the regulatory chain of Escherichia coli aspartate transcarbamoylase results in an extreme T-state structure.
Journal of molecular biology 1998;281(1):121-34.
1998: Dutta M; Kantrowitz E R
The influence of the regulatory chain amino acids Glu-62 and IIe-12 on the heterotropic properties of Escherichia coli aspartate transcarbamoylase.
Biochemistry 1998;37(24):8653-8.
1998: Tsuruta H; Vachette P; Kantrowitz E R
Direct observation of an altered quaternary-structure transition in a mutant aspartate transcarbamoylase.
Proteins 1998;31(4):383-90.
1998: Stec B; Hehir M J; Brennan C; Nolte M; Kantrowitz E R
Kinetic and X-ray structural studies of three mutant E. coli alkaline phosphatases: insights into the catalytic mechanism without the nucleophile Ser102.
Journal of molecular biology 1998;277(3):647-62.
1998: Sakash J B; Kantrowitz E R
The N-terminus of the regulatory chain of Escherichia coli aspartate transcarbamoylase is important for both nucleotide binding and heterotropic effects.
Biochemistry 1998;37(1):281-8.
1997: Murphy J E; Stec B; Ma L; Kantrowitz E R
Trapping and visualization of a covalent enzyme-phosphate intermediate.
Nature structural biology 1997;4(8):618-22.
1997: Lu G; Giroux E L; Kantrowitz E R
Importance of the dimer-dimer interface for allosteric signal transduction and AMP cooperativity of pig kidney fructose-1,6-bisphosphatase. Site-specific mutagenesis studies of Glu-192 and Asp-187 residues on the 190's loop.
The Journal of biological chemistry 1997;272(8):5076-81.
1996: Aucoin J M; Pishko E J; Baker D P; Kantrowitz E R
Engineered complementation in Escherichia coli aspartate transcarbamoylase. Heterotropic regulation by quaternary structure stabilization.
The Journal of biological chemistry 1996;271(47):29865-9.
1996: Lu G; Stec B; Giroux E L; Kantrowitz E R
Evidence for an active T-state pig kidney fructose 1,6-bisphosphatase: interface residue Lys-42 is important for allosteric inhibition and AMP cooperativity.
Protein science : a publication of the Protein Society 1996;5(11):2333-42.
1996: Baker D P; Fetler L; Vachette P; Kantrowitz E R
The allosteric activator ATP induces a substrate-dependent alteration of the quaternary structure of a mutant aspartate transcarbamoylase impaired in active site closure.
Protein science : a publication of the Protein Society 1996;5(11):2276-86.
1996: Stec B; Abraham R; Giroux E; Kantrowitz E R
Crystal structures of the active site mutant (Arg-243-->Ala) in the T and R allosteric states of pig kidney fructose-1,6-bisphosphatase expressed in Escherichia coli.
Protein science : a publication of the Protein Society 1996;5(8):1541-53.
1996: Tibbitts T T; Murphy J E; Kantrowitz E R
Kinetic and structural consequences of replacing the aspartate bridge by asparagine in the catalytic metal triad of Escherichia coli alkaline phosphatase.
Journal of molecular biology 1996;257(3):700-15.
1996: Ma L; Kantrowitz E R
Kinetic and X-ray structural studies of a mutant Escherichia coli alkaline phosphatase (His-412-->Gln) at one of the zinc binding sites.
Biochemistry 1996;35(7):2394-402.
1996: Kelley N; Giroux E L; Lu G; Kantrowitz E R
Glutamic acid residue 98 is critical for catalysis in pig kidney fructose-1,6-bisphosphatase.
Biochemical and biophysical research communications 1996;219(3):848-52.
1995: Murphy J E; Tibbitts T T; Kantrowitz E R
Mutations at positions 153 and 328 in Escherichia coli alkaline phosphatase provide insight towards the structure and function of mammalian and yeast alkaline phosphatases.
Journal of molecular biology 1995;253(4):604-17.
1995: Baker D P; Aucoin J M; Williams M K; deMello L A; Kantrowitz E R
Overexpression and purification of the trimeric aspartate transcarbamoylase from Bacillus subtilis.
Protein expression and purification 1995;6(5):679-84.
1995: Lu G; Williams M K; Giroux E L; Kantrowitz E R
Fructose-1,6-bisphosphatase: arginine-22 is involved in stabilization of the T allosteric state.
Biochemistry 1995;34(41):13272-7.
1995: Ma L; Tibbitts T T; Kantrowitz E R
Escherichia coli alkaline phosphatase: X-ray structural studies of a mutant enzyme (His-412-->Asn) at one of the catalytically important zinc binding sites.
Protein science : a publication of the Protein Society 1995;4(8):1498-506.
1995: Lee B H; Ley B W; Kantrowitz E R; O'Leary M H; Wedler F C
Domain closure in the catalytic chains of Escherichia coli aspartate transcarbamoylase influences the kinetic mechanism.
The Journal of biological chemistry 1995;270(26):15620-7.
1995: Wedler F C; Ley B W; Lee B H; O'Leary M H; Kantrowitz E R
L-aspartate association contributes to rate limitation and induction of the T-->R transition in Escherichia coli aspartate transcarbamoylase. Equilibrium exchanges and kinetic isotope effects with a Vmax-enhanced mutant, Asp-236-->Ala.
The Journal of biological chemistry 1995;270(17):9725-33.
1995: Baker D P; Fetler L; Keiser R T; Vachette P; Kantrowitz E R
Weakening of the interface between adjacent catalytic chains promotes domain closure in Escherichia coli aspartate transcarbamoylase.
Protein science : a publication of the Protein Society 1995;4(2):258-67.
1994: Ma L; Kantrowitz E R
Mutations at histidine 412 alter zinc binding and eliminate transferase activity in Escherichia coli alkaline phosphatase.
The Journal of biological chemistry 1994;269(50):31614-9.
1994: Giroux E; Williams M K; Kantrowitz E R
Shared active sites of fructose-1,6-bisphosphatase. Arginine 243 mediates substrate binding and fructose 2,6-bisphosphate inhibition.
The Journal of biological chemistry 1994;269(50):31404-9.
1994: Tibbitts T T; Xu X; Kantrowitz E R
Kinetics and crystal structure of a mutant Escherichia coli alkaline phosphatase (Asp-369-->Asn): a mechanism involving one zinc per active site.
Protein science : a publication of the Protein Society 1994;3(11):2005-14.
1994: Tauc P; Keiser R T; Kantrowitz E R; Vachette P
Glu-50 in the catalytic chain of Escherichia coli aspartate transcarbamoylase plays a crucial role in the stability of the R quaternary structure.
Protein science : a publication of the Protein Society 1994;3(11):1998-2004.
1994: Baker D P; Stebbins J W; DeSena E; Kantrowitz E R
Glutamic acid 86 is important for positioning the 80's loop and arginine 54 at the active site of Escherichia coli aspartate transcarbamoylase and for the structural stabilization of the C1-C2 interface.
The Journal of biological chemistry 1994;269(40):24608-14.
1994: Dembowski N J; Kantrowitz E R
The use of alanine scanning mutagenesis to determine the role of the N-terminus of the regulatory chain in the heterotropic mechanism of Escherichia coli aspartate transcarbamoylase.
Protein engineering 1994;7(5):673-9.
1994: Xu X; Qin X Q; Kantrowitz E R
Probing the role of histidine-372 in zinc binding and the catalytic mechanism of Escherichia coli alkaline phosphatase by site-specific mutagenesis.
Biochemistry 1994;33(8):2279-84.
1993: Murphy J E; Xu X; Kantrowitz E R
Conversion of a magnesium binding site into a zinc binding site by a single amino acid substitution in Escherichia coli alkaline phosphatase.
The Journal of biological chemistry 1993;268(29):21497-500.
1993: Xu X; Kantrowitz E R
Binding of magnesium in a mutant Escherichia coli alkaline phosphatase changes the rate-determining step in the reaction mechanism.
Biochemistry 1993;32(40):10683-91.
1993: Baker D P; Kantrowitz E R
The conserved residues glutamate-37, aspartate-100, and arginine-269 are important for the structural stabilization of Escherichia coli aspartate transcarbamoylase.
Biochemistry 1993;32(38):10150-8.
1993: Chaidaroglou A; Kantrowitz E R
The Ala-161-->Thr substitution in Escherichia coli alkaline phosphatase does not result in loss of enzymatic activity although the homologous mutation in humans causes hypophosphatasia.
Biochemical and biophysical research communications 1993;193(3):1104-9.
1993: Janeway C M; Xu X; Murphy J E; Chaidaroglou A; Kantrowitz E R
Magnesium in the active site of Escherichia coli alkaline phosphatase is important for both structural stabilization and catalysis.
Biochemistry 1993;32(6):1601-9.
1993: Dembowski N J; Kantrowitz E R
Overproduction and purification of the regulatory subunit of Escherichia coli aspartate transcarbamoylase.
Protein engineering 1993;6(1):123-7.
1992: Stebbins J W; Robertson D E; Roberts M F; Stevens R C; Lipscomb W N; Kantrowitz E R
Arginine 54 in the active site of Escherichia coli aspartate transcarbamoylase is critical for catalysis: a site-specific mutagenesis, NMR, and X-ray crystallographic study.
Protein science : a publication of the Protein Society 1992;1(11):1435-46.
1992: Xu X; Kantrowitz E R
The importance of aspartate 327 for catalysis and zinc binding in Escherichia coli alkaline phosphatase.
The Journal of biological chemistry 1992;267(23):16244-51.
1992: Williams M K; Kantrowitz E R
Isolation and sequence analysis of the cDNA for pig kidney fructose 1,6-bisphosphatase.
Proceedings of the National Academy of Sciences of the United States of America 1992;89(7):3080-2.
1992: Newton C J; Stevens R C; Kantrowitz E R
Importance of a conserved residue, aspartate-162, for the function of Escherichia coli aspartate transcarbamoylase.
Biochemistry 1992;31(11):3026-32.
1992: Stebbins J W; Kantrowitz E R
Conversion of the noncooperative Bacillus subtilis aspartate transcarbamoylase into a cooperative enzyme by a single amino acid substitution.
Biochemistry 1992;31(8):2328-32.
1992: Zhang Y; Kantrowitz E R
Probing the regulatory site of Escherichia coli aspartate transcarbamoylase by site-specific mutagenesis.
Biochemistry 1992;31(3):792-8.
1991: Zhang Y; Kantrowitz E R
The synergistic inhibition of Escherichia coli aspartate carbamoyltransferase by UTP in the presence of CTP is due to the binding of UTP to the low affinity CTP sites.
The Journal of biological chemistry 1991;266(33):22154-8.
1991: Xu X; Kantrowitz E R
A water-mediated salt link in the catalytic site of Escherichia coli alkaline phosphatase may influence activity.
Biochemistry 1991;30(31):7789-96.
1991: Stevens R C; Chook Y M; Cho C Y; Lipscomb W N; Kantrowitz E R
Escherichia coli aspartate carbamoyltransferase: the probing of crystal structure analysis via site-specific mutagenesis.
Protein engineering 1991;4(4):391-408.
1991: Xu W; Kantrowitz E R
Function of serine-52 and serine-80 in the catalytic mechanism of Escherichia coli aspartate transcarbamoylase.
Biochemistry 1991;30(9):2535-42.
1990: Tauc P; Vachette P; Middleton S A; Kantrowitz E R
Structural consequences of the replacement of Glu239 by Gln in the catalytic chain of Escherichia coli aspartate transcarbamylase.
Journal of molecular biology 1990;214(1):327-35.
1990: Stebbins J W; Zhang Y; Kantrowitz E R
Importance of residues Arg-167 and Gln-231 in both the allosteric and catalytic mechanisms of Escherichia coli aspartate transcarbamoylase.
Biochemistry 1990;29(16):3821-7.
1990: Dembowski N J; Newton C J; Kantrowitz E R
Function of serine-171 in domain closure, cooperativity, and catalysis in Escherichia coli aspartate transcarbamoylase.
Biochemistry 1990;29(15):3716-23.
1990: Newton C J; Kantrowitz E R
The regulatory subunit of Escherichia coli aspartate carbamoyltransferase may influence homotropic cooperativity and heterotropic interactions by a direct interaction with the loop containing residues 230-245 of the catalytic chain.
Proceedings of the National Academy of Sciences of the United States of America 1990;87(6):2309-13.
1990: Kantrowitz E R; Lipscomb W N
Escherichia coli aspartate transcarbamoylase: the molecular basis for a concerted allosteric transition.
Trends in biochemical sciences 1990;15(2):53-9.
1990: Newton C J; Kantrowitz E R
Importance of domain closure for homotropic cooperativity in Escherichia coli aspartate transcarbamylase.
Biochemistry 1990;29(6):1444-51.
1989: Xu W; Kantrowitz E R
Function of threonine-55 in the carbamoyl phosphate binding site of Escherichia coli aspartate transcarbamoylase.
Biochemistry 1989;28(26):9937-43.
1989: Chaidaroglou A; Kantrowitz E R
Alteration of aspartate 101 in the active site of Escherichia coli alkaline phosphatase enhances the catalytic activity.
Protein engineering 1989;3(2):127-32.
1989: Wedler F C; Hsuanyu Y C; Kantrowitz E R; Middleton S A
Regulatory behavior of Escherichia coli aspartate transcarbamylase altered by site-specific mutation of Tyr240----Phe in the catalytic chain.
The Journal of biological chemistry 1989;264(29):17266-74.
1989: Hsuanyu Y; Wedler F C; Kantrowitz E R; Middleton S A
Site-specific mutation of Tyr240----Phe in the catalytic chain of Escherichia coli aspartate transcarbamylase. Consequences for kinetic mechanism.
The Journal of biological chemistry 1989;264(29):17259-65.
1989: Stebbins J W; Kantrowitz E R
The importance of the link between Glu204 of the catalytic chain and Arg130 of the regulatory chain for the homotropic and heterotropic properties of Escherichia coli aspartate transcarbamoylase.
The Journal of biological chemistry 1989;264(25):14860-4.
1989: Zhang Y; Kantrowitz E R
Lysine-60 in the regulatory chain of Escherichia coli aspartate transcarbamoylase is important for the discrimination between CTP and ATP.
Biochemistry 1989;28(18):7313-8.
1989: Cherfils J; Sweet R M; Middleton S A; Kantrowitz E R; Tauc P; Vachette P
Structural consequences of a one atom mutation on aspartate transcarbamylase from E. coli.
FEBS letters 1989;247(2):361-6.
1989: Stebbins J W; Xu W; Kantrowitz E R
Three residues involved in binding and catalysis in the carbamyl phosphate binding site of Escherichia coli aspartate transcarbamylase.
Biochemistry 1989;28(6):2592-600.
1989: Hsuanyu Y; Wedler F C; Middleton S A; Kantrowitz E R
Kinetic consequences of site-specific mutation of Glu-239----Gln in E. coli aspartate transcarbamylase: comparison with catalytic subunits and Phe-240 mutant enzyme.
Biochimica et biophysica acta 1989;995(1):54-8.
1989: Gouaux J E; Lipscomb W N; Middleton S A; Kantrowitz E R
Structure of a single amino acid mutant of aspartate carbamoyltransferase at 2.5-A resolution: implications for the cooperative mechanism.
Biochemistry 1989;28(4):1798-803.
1989: Middleton S A; Stebbins J W; Kantrowitz E R
A loop involving catalytic chain residues 230-245 is essential for the stabilization of both allosteric forms of Escherichia coli aspartate transcarbamylase.
Biochemistry 1989;28(4):1617-26.
1988: Middleton S A; Kantrowitz E R
Function of arginine-234 and aspartic acid-271 in domain closure, cooperativity, and catalysis in Escherichia coli aspartate transcarbamylase.
Biochemistry 1988;27(23):8653-60.
1988: Chaidaroglou A; Brezinski D J; Middleton S A; Kantrowitz E R
Function of arginine-166 in the active site of Escherichia coli alkaline phosphatase.
Biochemistry 1988;27(22):8338-43.
1988: Xu W; Pitts M A; Middleton S A; Kelleher K S; Kantrowitz E R
Propagation of allosteric changes through the catalytic-regulatory interface of Escherichia coli aspartate transcarbamylase.
Biochemistry 1988;27(15):5507-15.
1988: Ladjimi M M; Kantrowitz E R
A possible model for the concerted allosteric transition in Escherichia coli aspartate transcarbamylase as deduced from site-directed mutagenesis studies.
Biochemistry 1988;27(1):276-83.
1988: Ladjimi M M; Middleton S A; Kelleher K S; Kantrowitz E R
Relationship between domain closure and binding, catalysis, and regulation in Escherichia coli aspartate transcarbamylase.
Biochemistry 1988;27(1):268-76.
1988: Zhang Y; Ladjimi M M; Kantrowitz E R
Site-directed mutagenesis of a residue located in the regulatory site of Escherichia coli aspartate transcarbamoylase. Involvement of lysine 94 in effector binding and the allosteric mechanism.
The Journal of biological chemistry 1988;263(3):1320-4.
1987: Ladjimi M M; Kantrowitz E R
Catalytic-regulatory subunit interactions and allosteric effects in aspartate transcarbamylase.
The Journal of biological chemistry 1987;262(1):312-8.
1986: Middleton S A; Kantrowitz E R
Importance of the loop at residues 230-245 in the allosteric interactions of Escherichia coli aspartate carbamoyltransferase.
Proceedings of the National Academy of Sciences of the United States of America 1986;83(16):5866-70.
1986: Smith K A; Nowlan S F; Middleton S A; O'Donovan C; Kantrowitz E R
Involvement of tryptophan 209 in the allosteric interactions of Escherichia coli aspartate transcarbamylase using single amino acid substitution mutants.
Journal of molecular biology 1986;189(1):227-38.
1985: Nowlan S F; Kantrowitz E R
Superproduction and rapid purification of Escherichia coli aspartate transcarbamylase and its catalytic subunit under extreme derepression of the pyrimidine pathway.
The Journal of biological chemistry 1985;260(27):14712-6.

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