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Ryushi Kawakami

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Takenori Shibahara; Takenori Satomura; Ryushi Kawakami; Toshihisa Ohshima; Haruhiko Sakuraba
Crystallization and preliminary X-ray analysis of a dye-linked D-lactate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix.
Hitoshi Sakuraba; Kazunari Yoneda; Kumiko Yoshihara; Kyoko Satoh; Ryushi Kawakami; Yoshihiro Uto; Hideaki Tsuge; Katsuyuki Takahashi; Hitoshi Hori; Toshihisa Ohshima
Sequential aldol condensation catalyzed by hyperthermophilic 2-deoxy-d-ribose-5-phosphate aldolase.
Ryushi Kawakami; Hitoshi Sakuraba; Toshihisa Ohshima
Gene cloning and characterization of the very large NAD-dependent l-glutamate dehydrogenase from the psychrophile Janthinobacterium lividum, isolated from cold soil.

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2011: Takenori Shibahara; Takenori Satomura; Ryushi Kawakami; Toshihisa Ohshima; Haruhiko Sakuraba
Crystallization and preliminary X-ray analysis of a dye-linked D-lactate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix.
Acta crystallographica. Section F, Structural biology and crystallization communications 2011;67(Pt 11):1425-7.
2007: Hitoshi Sakuraba; Kazunari Yoneda; Kumiko Yoshihara; Kyoko Satoh; Ryushi Kawakami; Yoshihiro Uto; Hideaki Tsuge; Katsuyuki Takahashi; Hitoshi Hori; Toshihisa Ohshima
Sequential aldol condensation catalyzed by hyperthermophilic 2-deoxy-d-ribose-5-phosphate aldolase.
Applied and environmental microbiology 2007;73(22):7427-34.
2007: Ryushi Kawakami; Hitoshi Sakuraba; Toshihisa Ohshima
Gene cloning and characterization of the very large NAD-dependent l-glutamate dehydrogenase from the psychrophile Janthinobacterium lividum, isolated from cold soil.
Journal of bacteriology 2007;189(15):5626-33.
2007: Toshihisa Ohshima; Ryushi Kawakami; Yuichiro Kanai; Shuichiro Goda; Hitoshi Sakuraba
Gene expression and characterization of 2-keto-3-deoxygluconate kinase, a key enzyme in the modified Entner-Doudoroff pathway of the aerobic and acidophilic hyperthermophile Sulfolobus tokodaii.
Protein expression and purification 2007;54(1):73-8.
2006: Kazunari Yoneda; Ryushi Kawakami; Yuya Tagashira; Hitoshi Sakuraba; Shuichiro Goda; Toshihisa Ohshima
The first archaeal L-aspartate dehydrogenase from the hyperthermophile Archaeoglobus fulgidus: gene cloning and enzymological characterization.
Biochimica et biophysica acta 2006;1764(6):1087-93.
2006: Hideaki Tsuge; Kenji Aki; Nobuhiko Katanuma; Ryushi Kawakami; Hitoshi Sakuraba; Toshihisa Ohshima
[Novel diflavin-containing dehydrogenase family: FAD, FMN and ATP-containing L-proline dehydrogenase].
Seikagaku. The Journal of Japanese Biochemical Society 2006;78(1):41-5.
2005: Shuichiro Goda; Masaki Kojima; Yoshimi Nishikawa; Chizu Kujo; Ryushi Kawakami; Seiki Kuramitsu; Hitoshi Sakuraba; Yuzuru Hiragi; Toshihisa Ohshima
Intersubunit interaction induced by subunit rearrangement is essential for the catalytic activity of the hyperthermophilic glutamate dehydrogenase from Pyrobaculum islandicum.
Biochemistry 2005;44(46):15304-13.
2005: Hideaki Tsuge; Ryushi Kawakami; Hitoshi Sakuraba; Hideo Ago; Masashi Miyano; Kenji Aki; Nobuhiko Katunuma; Toshihisa Ohshima
Crystal structure of a novel FAD-, FMN-, and ATP-containing L-proline dehydrogenase complex from Pyrococcus horikoshii.
The Journal of biological chemistry 2005;280(35):31045-9.
2005: Ryushi Kawakami; Hitoshi Sakuraba; Hideaki Tsuge; Shuichiro Goda; Nobuhiko Katunuma; Toshihisa Ohshima
A second novel dye-linked L-proline dehydrogenase complex is present in the hyperthermophilic archaeon Pyrococcus horikoshii OT-3.
The FEBS journal 2005;272(16):4044-54.
2005: Hitoshi Sakuraba; Ryushi Kawakami; Toshihisa Ohshima
First archaeal inorganic polyphosphate/ATP-dependent NAD kinase, from hyperthermophilic archaeon Pyrococcus horikoshii: cloning, expression, and characterization.
Applied and environmental microbiology 2005;71(8):4352-8.
2005: Yasuhiro Shimizu; Hitoshi Sakuraba; Ryushi Kawakami; Shuichiro Goda; Yutaka Kawarabayasi; Toshihisa Ohshima
L-Threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus horikoshii OT3: gene cloning and enzymatic characterization.
Extremophiles : life under extreme conditions 2005;9(4):317-24.
2004: Ryushi Kawakami; Hitoshi Sakuraba; Shintaro Kamohara; Shuichiro Goda; Yutaka Kawarabayasi; Toshihisa Ohshima
Oxidative stress response in an anaerobic hyperthermophilic archaeon: presence of a functional peroxiredoxin in Pyrococcus horikoshii.
Journal of biochemistry 2004;136(4):541-7.
2004: Hitoshi Sakuraba; Ryushi Kawakami; Hajime Takahashi; Toshihisa Ohshima
Novel archaeal alanine:glyoxylate aminotransferase from Thermococcus litoralis.
Journal of bacteriology 2004;186(16):5513-8.
2004: Ryushi Kawakami; Hitoshi Sakuraba; Toshihisa Ohshima
Gene and primary structures of dye-linked L-proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex.
Extremophiles : life under extreme conditions 2004;8(2):99-108.
2003: Hitoshi Sakuraba; Hideaki Tsuge; Ikuko Shimoya; Ryushi Kawakami; Shuichiro Goda; Yutaka Kawarabayasi; Nobuhiko Katunuma; Hideo Ago; Masashi Miyano; Toshihisa Ohshima
The first crystal structure of archaeal aldolase. Unique tetrameric structure of 2-deoxy-d-ribose-5-phosphate aldolase from the hyperthermophilic archaea Aeropyrum pernix.
The Journal of biological chemistry 2003;278(12):10799-806.
2002: Hitoshi Sakuraba; Takenori Satomura; Ryushi Kawakami; Sanae Yamamoto; Yutaka Kawarabayasi; Hisasi Kikuchi; Toshihisa Ohshima
L-aspartate oxidase is present in the anaerobic hyperthermophilic archaeon Pyrococcus horikoshii OT-3: characteristics and role in the de novo biosynthesis of nicotinamide adenine dinucleotide proposed by genome sequencing.
Extremophiles : life under extreme conditions 2002;6(4):275-81.
2002: Hitoshi Sakuraba; Issei Yoshioka; Shinji Koga; Mamoru Takahashi; Yuki Kitahama; Takenori Satomura; Ryushi Kawakami; Toshihisa Ohshima
ADP-dependent glucokinase/phosphofructokinase, a novel bifunctional enzyme from the hyperthermophilic archaeon Methanococcus jannaschii.
The Journal of biological chemistry 2002;277(15):12495-8.
2002: Takenori Satomura; Ryushi Kawakami; Hitoshi Sakuraba; Toshihisa Ohshima
Dye-linked D-proline dehydrogenase from hyperthermophilic archaeon Pyrobaculum islandicum is a novel FAD-dependent amino acid dehydrogenase.
The Journal of biological chemistry 2002;277(15):12861-7.
2001: Hitoshi Sakuraba; Y Takamatsu; Takenori Satomura; Ryushi Kawakami; Toshihisa Ohshima
Purification, characterization, and application of a novel dye-linked L-proline dehydrogenase from a hyperthermophilic archaeon, Thermococcus profundus.
Applied and environmental microbiology 2001;67(4):1470-5.