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Yutaka Kawarabayasi

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Digvijay Verma; Yutaka Kawarabayasi; Kentaro Miyazaki; Tulasi Satyanarayana
Cloning, expression and characteristics of a novel alkalistable and thermostable xylanase encoding gene (Mxyl) retrieved from compost-soil metagenome.
Hironaga Akita; Katsumi Doi; Yutaka Kawarabayasi; Toshihisa Ohshima
Creation of a thermostable NADP⁺-dependent D-amino acid dehydrogenase from Ureibacillus thermosphaericus strain A1 meso-diaminopimelate dehydrogenase by site-directed mutagenesis.
Syuji Yamazaki; Shigeo Yoshinari; Kiyoshi Kita; Yoh-ichi Watanabe; Yutaka Kawarabayasi
Identification of an entire set of tRNA molecules and characterization of cleavage sites of the intron-containing tRNA precursors in acidothermophilic crenarchaeon Sulfolobus tokodaii strain7.

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2013: Digvijay Verma; Yutaka Kawarabayasi; Kentaro Miyazaki; Tulasi Satyanarayana
Cloning, expression and characteristics of a novel alkalistable and thermostable xylanase encoding gene (Mxyl) retrieved from compost-soil metagenome.
PloS one 2013;8(1):e52459.
2012: Hironaga Akita; Katsumi Doi; Yutaka Kawarabayasi; Toshihisa Ohshima
Creation of a thermostable NADP⁺-dependent D-amino acid dehydrogenase from Ureibacillus thermosphaericus strain A1 meso-diaminopimelate dehydrogenase by site-directed mutagenesis.
Biotechnology letters 2012;34(9):1693-9.
2011: Syuji Yamazaki; Shigeo Yoshinari; Kiyoshi Kita; Yoh-ichi Watanabe; Yutaka Kawarabayasi
Identification of an entire set of tRNA molecules and characterization of cleavage sites of the intron-containing tRNA precursors in acidothermophilic crenarchaeon Sulfolobus tokodaii strain7.
Gene 2011;489(2):103-10.
2011: Yutaka Kawarabayasi
[Uniqueness of the metabolic pathway of carbohydrate in thermophilic archaea].
Seikagaku. The Journal of Japanese Biochemical Society 2011;83(12):1117-21.
2011: Tomoko Mase; Keiko Kubota; Ken-ichi Miyazono; Yutaka Kawarabayasi; Masaru Tanokura
Structure of flap endonuclease 1 from the hyperthermophilic archaeon Desulfurococcus amylolyticus.
Acta crystallographica. Section F, Structural biology and crystallization communications 2011;67(Pt 2):209-13.
2010: Zilian Zhang; Jun-ichi Akutsu; Yutaka Kawarabayasi
Identification of novel acetyltransferase activity on the thermostable protein ST0452 from Sulfolobus tokodaii strain 7.
Journal of bacteriology 2010;192(13):3287-93.
2010: Hidehiko Hirakawa; Noriho Kamiya; Yutaka Kawarabayasi; Teruyuki Nagamune
Artificial self-sufficient P450 in reversed micelles.
Molecules (Basel, Switzerland) 2010;15(5):2935-48.
2009: Md Murad Hossain; Yutaka Kawarabayasi; Makoto Kimura; Yoshimitsu Kakuta
Expression and functional analysis of a predicted AtsG arylsulphatase identified from Mycobacterium tuberculosis genomic data.
Journal of biochemistry 2009;146(6):767-9.
2009: Shingo Kato; Katsunori Yanagawa; Michinari Sunamura; Yoshinori Takano; Jun-Ichiro Ishibashi; Takeshi Kakegawa; Motoo Utsumi; Toshiro Yamanaka; Tomohiro Toki; Takuroh Noguchi; Kensei Kobayashi; Arimichi Moroi; Hiroyuki Kimura; Yutaka Kawarabayasi; Katsumi Marumo; Tetsuro Urabe; Akihiko Yamagishi
Abundance of Zetaproteobacteria within crustal fluids in back-arc hydrothermal fields of the Southern Mariana Trough.
Environmental microbiology 2009;11(12):3210-22.
2009: Tomoko Mase; Keiko Kubota; Ken-ichi Miyazono; Yutaka Kawarabayasi; Masaru Tanokura
Crystallization and preliminary X-ray analysis of flap endonuclease 1 (FEN1) from Desulfurococcus amylolyticus.
Acta crystallographica. Section F, Structural biology and crystallization communications 2009;65(Pt 9):923-5.
2009: Hiroaki Matsukawa; Takeshi Yamagami; Yutaka Kawarabayasi; Yukiko Miyashita; Masaaki Takahashi; Yoshizumi Ishino
A useful strategy to construct DNA polymerases with different properties by using genetic resources from environmental DNA.
Genes & genetic systems 2009;84(1):3-13.
2009: Junji Itou; Mikita Suyama; Yukio Imamura; Tomonori Deguchi; Kazuhiro Fujimori; Shunsuke Yuba; Yutaka Kawarabayasi; Takashi Kawasaki
Functional and comparative genomics analyses of pmp22 in medaka fish.
BMC neuroscience 2009;10():60.
2008: Takashi Kawasaki; Kotaro Saito; Tomonori Deguchi; Kazuhiro Fujimori; Mika Tadokoro; Shunsuke Yuba; Hajime Ohgushi; Yutaka Kawarabayasi
Pharmacological characterization of isoproterenol-treated medaka fish.
Pharmacological research : the official journal of the Italian Pharmacological Society 2008;58(5-6):348-55.
2008: Ken-ichi Miyazono; Masanari Tsujimura; Yutaka Kawarabayasi; Masaru Tanokura
Crystal structure of STS042, a stand-alone RAM module protein, from hyperthermophilic archaeon Sulfolobus tokodaii strain 7.
Proteins 2008;71(3):1557-62.
2007: Ken-ichi Miyazono; Masanari Tsujimura; Yutaka Kawarabayasi; Masaru Tanokura
Crystal structure of an archaeal homologue of multidrug resistance repressor protein, EmrR, from hyperthermophilic archaea Sulfolobus tokodaii strain 7.
Proteins 2007;67(4):1138-46.
2007: Kaori Imamura; Kenzo Fukunaga; Yutaka Kawarabayasi; Yoshizumi Ishino
Specific interactions of three proliferating cell nuclear antigens with replication-related proteins in Aeropyrum pernix.
Molecular microbiology 2007;64(2):308-18.
2007: Zilian Zhang; Jun-ichi Akutsu; Masanari Tsujimura; Yutaka Kawarabayasi
Increasing in archaeal GlcNAc-1-P uridyltransferase activity by targeted mutagenesis while retaining its extreme thermostability.
Journal of biochemistry 2007;141(4):553-62.
2007: Masahiro Fujihashi; Nobutaka Numoto; Yukiko Kobayashi; Akira Mizushima; Masanari Tsujimura; Akira Nakamura; Yutaka Kawarabayasi; Kunio Miki
Crystal structure of archaeal photolyase from Sulfolobus tokodaii with two FAD molecules: implication of a novel light-harvesting cofactor.
Journal of molecular biology 2007;365(4):903-10.
2006: Shinji Sueda; Yan-Qiu Li; Hiroki Kondo; Yutaka Kawarabayasi
Substrate specificity of archaeon Sulfolobus tokodaii biotin protein ligase.
Biochemical and biophysical research communications 2006;344(1):155-9.
2006: Yan-Qiu Li; Shinji Sueda; Hiroki Kondo; Yutaka Kawarabayasi
A unique biotin carboxyl carrier protein in archaeon Sulfolobus tokodaii.
FEBS letters 2006;580(6):1536-40.
2006: Yosuke Koga; Mami Ohga; Masanari Tsujimura; Hiroyuki Morii; Yutaka Kawarabayasi
Identification of sn-glycerol-1-phosphate dehydrogenase activity from genomic information on a hyperthermophilic archaeon, Sulfolobus tokodaii strain 7.
Bioscience, biotechnology, and biochemistry 2006;70(1):282-5.
2005: Jun-ichi Akutsu; Zilian Zhang; Masanari Tsujimura; Mayumi Sasaki; Masafumi Yohda; Yutaka Kawarabayasi
Characterization of a thermostable enzyme with phosphomannomutase/phosphoglucomutase activities from the hyperthermophilic archaeon Pyrococcus horikoshii OT3.
Journal of biochemistry 2005;138(2):159-66.
2005: Yasuhiro Shimizu; Hitoshi Sakuraba; Ryushi Kawakami; Shuichiro Goda; Yutaka Kawarabayasi; Toshihisa Ohshima
L-Threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus horikoshii OT3: gene cloning and enzymatic characterization.
Extremophiles : life under extreme conditions 2005;9(4):317-24.
2005: Izumi Orita; Hiroya Yurimoto; Reiko Hirai; Yutaka Kawarabayasi; Yasuyoshi Sakai; Nobuo Kato
The archaeon Pyrococcus horikoshii possesses a bifunctional enzyme for formaldehyde fixation via the ribulose monophosphate pathway.
Journal of bacteriology 2005;187(11):3636-42.
2005: Shuichiro Goda; Hitoshi Sakuraba; Yutaka Kawarabayasi; Toshihisa Ohshima
The first archaeal agmatinase from anaerobic hyperthermophilic archaeon Pyrococcus horikoshii: cloning, expression, and characterization.
Biochimica et biophysica acta 2005;1748(1):110-5.
2005: Zilian Zhang; Masanari Tsujimura; Jun-ichi Akutsu; Mayumi Sasaki; Hideji Tajima; Yutaka Kawarabayasi
Identification of an extremely thermostable enzyme with dual sugar-1-phosphate nucleotidylyltransferase activities from an acidothermophilic archaeon, Sulfolobus tokodaii strain 7.
The Journal of biological chemistry 2005;280(10):9698-705.
2005: Tomokazu Shirai; Akinori Nakato; Noriyuki Izutani; Keisuke Nagahisa; Suteaki Shioya; Eiichiro Kimura; Yutaka Kawarabayasi; Akihiko Yamagishi; Takashi Gojobori; Hiroshi Shimizu
Comparative study of flux redistribution of metabolic pathway in glutamate production by two coryneform bacteria.
Metabolic engineering 2005;7(2):59-69.
2005: Syuji Yamazaki; Hisashi Kikuchi; Yutaka Kawarabayasi
Characterization of a whole set of tRNA molecules in an aerobic hyper-thermophilic Crenarchaeon, Aeropyrum pernix K1.
DNA research : an international journal for rapid publication of reports on genes and genomes 2005;12(6):403-16.
2004: Shaobin Hou; Jimmy Saw; Kit Shan Lee; Tracey Allen K Freitas; Claude Belisle; Yutaka Kawarabayasi; Stuart P Donachie; Alla Pikina; Michael Galperin; Eugene V Koonin; Kira S Makarova; Marina V Omelchenko; Alexei Sorokin; Yuri I Wolf; Qing X Li; Young-Soo Keum; Sonia Campbell; Judith Denery; Shin-Ichi Aizawa; Satoshi Shibata; Alexander Malahoff; Maqsudul Alam
Genome sequence of the deep-sea gamma-proteobacterium Idiomarina loihiensis reveals amino acid fermentation as a source of carbon and energy.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(52):18036-41.
2004: Ryushi Kawakami; Hitoshi Sakuraba; Shintaro Kamohara; Shuichiro Goda; Yutaka Kawarabayasi; Toshihisa Ohshima
Oxidative stress response in an anaerobic hyperthermophilic archaeon: presence of a functional peroxiredoxin in Pyrococcus horikoshii.
Journal of biochemistry 2004;136(4):541-7.
2004: John Lally; Matthew Newman; Judith Murray-Rust; Andrew Fadden; Yutaka Kawarabayasi; Neil McDonald
Crystallization of the xeroderma pigmentosum group F endonuclease from Aeropyrum pernix.
Acta crystallographica. Section D, Biological crystallography 2004;60(Pt 9):1658-61.
2004: Yousuke Nishio; Yoji Nakamura; Yoshihiro Usuda; Shinichi Sugimoto; Kazuhiko Matsui; Yutaka Kawarabayasi; Hisashi Kikuchi; Takashi Gojobori; Kazuho Ikeo
Evolutionary process of amino acid biosynthesis in Corynebacterium at the whole genome level.
Molecular biology and evolution 2004;21(9):1683-91.
2004: Yusuke Oku; Akashi Ohtaki; Shigehiro Kamitori; Nobuhumi Nakamura; Masafumi Yohda; Hiroyuki Ohno; Yutaka Kawarabayasi
Structure and direct electrochemistry of cytochrome P450 from the thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain 7.
Journal of inorganic biochemistry 2004;98(7):1194-9.
2004: Masanari Tsujimura; Yutaka Kawarabayasi
[Comprehensive expression of thermo-stable proteins in acidothermophilic archaea].
Seikagaku. The Journal of Japanese Biochemical Society 2004;76(2):149-54.
2004: Keisuke Usui; Noriyuki Ishii; Yutaka Kawarabayasi; Masafumi Yohda
Expression and biochemical characterization of two small heat shock proteins from the thermoacidophilic crenarchaeon Sulfolobus tokodaii strain 7.
Protein science : a publication of the Protein Society 2004;13(1):134-44.
2003: Yousuke Nishio; Yoji Nakamura; Yutaka Kawarabayasi; Yoshihiro Usuda; Eiichiro Kimura; Shinichi Sugimoto; Kazuhiko Matsui; Akihiko Yamagishi; Hisashi Kikuchi; Kazuho Ikeo; Takashi Gojobori
Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens.
Genome research 2003;13(7):1572-9.
2003: Hiroki Higashibata; Hisasi Kikuchi; Yutaka Kawarabayasi; Ikuo Matsui
Helicase and nuclease activities of hyperthermophile Pyrococcus horikoshii Dna2 inhibited by substrates with RNA segments at 5'-end.
The Journal of biological chemistry 2003;278(18):15983-90.
2003: Hitoshi Sakuraba; Hideaki Tsuge; Ikuko Shimoya; Ryushi Kawakami; Shuichiro Goda; Yutaka Kawarabayasi; Nobuhiko Katunuma; Hideo Ago; Masashi Miyano; Toshihisa Ohshima
The first crystal structure of archaeal aldolase. Unique tetrameric structure of 2-deoxy-d-ribose-5-phosphate aldolase from the hyperthermophilic archaea Aeropyrum pernix.
The Journal of biological chemistry 2003;278(12):10799-806.
2003: Hitoshi Sakuraba; Yuri Mitani; Shuichiro Goda; Yutaka Kawarabayasi; Toshihisa Ohshima
Cloning, expression, and characterization of the first archaeal ATP-dependent glucokinase from aerobic hyperthermophilic archaeon Aeropyrum pernix.
Journal of biochemistry 2003;133(2):219-24.
2003: Junji Yokozawa; Koji Okamoto; Yutaka Kawarabayasi; Atsushi Kuno; Tsunemi Hasegawa
Molecular recognition of proline tRNA by prolyl-tRNA synthetase from hyperthermophilic archaeon, Aeropyrum pernix K1.
Nucleic acids research. Supplement (2001) 2003;(3):247-8.
2002: Hitoshi Sakuraba; Takenori Satomura; Ryushi Kawakami; Sanae Yamamoto; Yutaka Kawarabayasi; Hisasi Kikuchi; Toshihisa Ohshima
L-aspartate oxidase is present in the anaerobic hyperthermophilic archaeon Pyrococcus horikoshii OT-3: characteristics and role in the de novo biosynthesis of nicotinamide adenine dinucleotide proposed by genome sequencing.
Extremophiles : life under extreme conditions 2002;6(4):275-81.
2002: Yoh-ichi Watanabe; Shin-ichi Yokobori; Yutaka Kawarabayasi
[Intron and pre-mRNA splicing of bacteria and Archaea].
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 2002;47(7):833-6.
2002: Kazuhiko Ishikawa; Ikuo Matsui; Françoise Payan; Christian Cambillau; Hiroyasu Ishida; Yutaka Kawarabayasi; Hisasi Kikuchi; Alain Roussel
A hyperthermostable D-ribose-5-phosphate isomerase from Pyrococcus horikoshii characterization and three-dimensional structure.
Structure (London, England : 1993) 2002;10(6):877-86.
2002: Lijun Liu; Kousuke Iwata; Akiko Kita; Yutaka Kawarabayasi; Masafumi Yohda; Kunio Miki
Crystal structure of aspartate racemase from Pyrococcus horikoshii OT3 and its implications for molecular mechanism of PLP-independent racemization.
Journal of molecular biology 2002;319(2):479-89.
2002: Mina Okochi; Takao Yoshida; Tadashi Maruyama; Yutaka Kawarabayasi; Hisashi Kikuchi; Masafumi Yohda
Pyrococcus prefoldin stabilizes protein-folding intermediates and transfers them to chaperonins for correct folding.
Biochemical and biophysical research communications 2002;291(4):769-74.
2002: Katsuya Daimon; Yutaka Kawarabayasi; Hisashi Kikuchi; Yoshihiko Sako; Yoshizumi Ishino
Three proliferating cell nuclear antigen-like proteins found in the hyperthermophilic archaeon Aeropyrum pernix: interactions with the two DNA polymerases.
Journal of bacteriology 2002;184(3):687-94.
2002: Yoh-ichi Watanabe; Shin-ichi Yokobori; Toshiro Inaba; Akihiko Yamagishi; Tairo Oshima; Yutaka Kawarabayasi; Hisasi Kikuchi; Kiyoshi Kita
Introns in protein-coding genes in Archaea.
FEBS letters 2002;510(1-2):27-30.
2002: Jun Iwaki; Haruichi Asahara; Yoshiyuki Nagaoka; Junji Yokozawa; Takuya Umehara; Yutaka Kawarabayasi; Yoshinori Koyama; Yoshihiko Sako; Atsushi Kuno; Tsunemi Hasegawa
Differences in tyrosine tRNA identity between Escherichia coli and archaeon, Aeropyrum pernix K1.
Nucleic acids research. Supplement (2001) 2002;(2):225-6.
2002: Yoshiyuki Nagaoka; Junji Yokozawa; Takuya Umehara; Jun Iwaki; Koji Okamoto; Yutaka Kawarabayasi; Yoshinori Koyama; Yoshihiko Sako; Takayoshi Wakagi; Atsushi Kuno; Tsunemi Hasegawa
Molecular recognition of threonine tRNA by threonyl-tRNA synthetase from an extreme thermophilic archaeon, Aeropyrum pernix K1.
Nucleic acids research. Supplement (2001) 2002;(2):81-2.
2001: Lijun Liu; Kousuke Iwata; Yutaka Kawarabayasi; H Kikuchi; Akiko Kita; Masafumi Yohda; Kunio Miki
Crystallization and preliminary X-ray analysis of aspartate racemase from Pyrococcus horikoshii OT3.
Acta crystallographica. Section D, Biological crystallography 2001;57(Pt 11):1674-6.
2001: Yutaka Kawarabayasi; Y Hino; H Horikawa; K Jin-no; M Takahashi; M Sekine; S Baba; A Ankai; H Kosugi; A Hosoyama; S Fukui; Y Nagai; K Nishijima; R Otsuka; H Nakazawa; M Takamiya; Y Kato; T Yoshizawa; T Tanaka; Y Kudoh; J Yamazaki; N Kushida; A Oguchi; K Aoki; S Masuda; M Yanagii; M Nishimura; A Yamagishi; T Oshima; Hisashi Kikuchi
Complete genome sequence of an aerobic thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain7.
DNA research : an international journal for rapid publication of reports on genes and genomes 2001;8(4):123-40.
2001: R Zheng; Eriko Matsui; Y Shen; Krishnasastry V Musti; Y Feng; S Darnis; Yutaka Kawarabayasi; H Kikuchi; Kazuaki Harata; Ikuo Matsui
The novel function of a short region K253xRxxxD259 conserved in the exonuclease domain of hyperthermostable DNA polymerase I from Pyrococcus horikoshii.
Extremophiles : life under extreme conditions 2001;5(2):111-7.
2001: K Ishikawa; H Ishida; Ikuo Matsui; Yutaka Kawarabayasi; H Kikuchi
Novel bifunctional hyperthermostable carboxypeptidase/aminoacylase from Pyrococcus horikoshii OT3.
Applied and environmental microbiology 2001;67(2):673-9.
2001: Junji Yokozawa; Yoshiyuki Nagaoka; Takuya Umehara; Jun Iwaki; Yutaka Kawarabayasi; Yoshinori Koyama; Yoshihiko Sako; Takayoshi Wakagi; Atsushi Kuno; Tsunemi Hasegawa
Recognition of tRNA by aminoacyl-tRNA synthetase from hyperthermophilic archaea, Aeropyrum pernix K1.
Nucleic acids research. Supplement (2001) 2001;(1):117-8.
2000: Mohammad W Bhuiya; Hitoshi Sakuraba; Chizu Kujo; Naoki Nunoura-Kominato; Yutaka Kawarabayasi; H Kikuchi; Toshihisa Ohshima
Glutamate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix K1: enzymatic characterization, identification of the encoding gene, and phylogenetic implications.
Extremophiles : life under extreme conditions 2000;4(6):333-41.
2000: Ikuo Matsui; Eriko Matsui; Y Sakai; H Kikuchi; Yutaka Kawarabayasi; H Ura; S i Kawaguchi; Seiki Kuramitsu; Kazuaki Harata
The molecular structure of hyperthermostable aromatic aminotransferase with novel substrate specificity from Pyrococcus horikoshii.
The Journal of biological chemistry 2000;275(7):4871-9.
2000: Ikuo Matsui; Y Sakai; Eriko Matsui; H Kikuchi; Yutaka Kawarabayasi; K Honda
Novel substrate specificity of a membrane-bound beta-glycosidase from the hyperthermophilic archaeon Pyrococcus horikoshii.
FEBS letters 2000;467(2-3):195-200.
1999: Yutaka Kawarabayasi; Y Hino; H Horikawa; S Yamazaki; Y Haikawa; K Jin-no; M Takahashi; M Sekine; S Baba; A Ankai; H Kosugi; A Hosoyama; S Fukui; Y Nagai; K Nishijima; H Nakazawa; M Takamiya; S Masuda; T Funahashi; T Tanaka; Y Kudoh; J Yamazaki; N Kushida; A Oguchi; Hisashi Kikuchi
Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1.
DNA research : an international journal for rapid publication of reports on genes and genomes 1999;6(2):83-101, 145-52.
1999: S Ando; K Ishikawa; H Ishida; Yutaka Kawarabayasi; H Kikuchi; Y Kosugi
Thermostable aminopeptidase from Pyrococcus horikoshii.
FEBS letters 1999;447(1):25-8.
1998: K Ishikawa; H Ishida; Y Koyama; Yutaka Kawarabayasi; J Kawahara; Eriko Matsui; Ikuo Matsui
Acylamino acid-releasing enzyme from the thermophilic archaeon Pyrococcus horikoshii.
The Journal of biological chemistry 1998;273(28):17726-31.
1998: Yutaka Kawarabayasi; M Sawada; H Horikawa; Y Haikawa; Y Hino; S Yamamoto; M Sekine; S Baba; H Kosugi; A Hosoyama; Y Nagai; M Sakai; K Ogura; R Otsuka; H Nakazawa; M Takamiya; Y Ohfuku; T Funahashi; T Tanaka; Y Kudoh; J Yamazaki; N Kushida; A Oguchi; K Aoki; Hisashi Kikuchi
Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3 (supplement).
DNA research : an international journal for rapid publication of reports on genes and genomes 1998;5(2):147-55.
1998: Yutaka Kawarabayasi; M Sawada; H Horikawa; Y Haikawa; Y Hino; S Yamamoto; M Sekine; S Baba; H Kosugi; A Hosoyama; Y Nagai; M Sakai; K Ogura; R Otsuka; H Nakazawa; M Takamiya; Y Ohfuku; T Funahashi; T Tanaka; Y Kudoh; J Yamazaki; N Kushida; A Oguchi; K Aoki; Hisashi Kikuchi
Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3.
DNA research : an international journal for rapid publication of reports on genes and genomes 1998;5(2):55-76.