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Hiroaki Kawasaki

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Masanori Ito; Makoto Miyagishi; Chisato Murata; Hiroaki Kawasaki; Tadashi Baba; Chikashi Tachi; Kazunari Taira
Down-regulation of endogenous Wt1 expression by Sry transgene in the murine embryonic mesonephros-derived M15 cell line.
Yoko Fukuda; Hiroaki Kawasaki; Kazunari Taira
Construction of microRNA-containing vectors for expression in mammalian cells.
Hiroaki Kawasaki; Kazunari Taira
Transcriptional gene silencing by short interfering RNAs.

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2006: Masanori Ito; Makoto Miyagishi; Chisato Murata; Hiroaki Kawasaki; Tadashi Baba; Chikashi Tachi; Kazunari Taira
Down-regulation of endogenous Wt1 expression by Sry transgene in the murine embryonic mesonephros-derived M15 cell line.
The Journal of reproduction and development 2006;52(3):415-27.
2006: Yoko Fukuda; Hiroaki Kawasaki; Kazunari Taira
Construction of microRNA-containing vectors for expression in mammalian cells.
Methods in molecular biology (Clifton, N.J.) 2006;338():167-73.
2005: Hiroaki Kawasaki; Kazunari Taira
Transcriptional gene silencing by short interfering RNAs.
Current opinion in molecular therapeutics 2005;7(2):125-31.
2005: Hiroaki Kawasaki; Kazunari Taira; Kevin V Morris
siRNA induced transcriptional gene silencing in mammalian cells.
Cell cycle (Georgetown, Tex.) 2005;4(3):442-8.
2005: Yoko Fukuda; Hiroaki Kawasaki; Kazunari Taira
Exploration of human miRNA target genes in neuronal differentiation.
Nucleic acids symposium series (2004) 2005;(49):341-2.
2005: Makoto Miyagishi; Sahohime Matsumoto; Hideo Akashi; Hiroaki Kawasaki; Taro Fukao; Yoko Fukuda; Masayuki Sano; Yoshio Kato; Yasuomi Takagi; Yoshiyuki Tanaka; Masaki Warashina; Tomoko Kuwabara; Shinya Y Sawata; Yutaka Ikeda; Shun-ichi Kawahara; Kaul C Sunil; Renu Wadhwa; Kazunari Taira
Chemistry-based RNA technologies: demonstration of usefulness of libraries of ribozymes and short hairpin RNAs (shRNAs).
Nucleic acids symposium series (2004) 2005;(49):91-2.
2004: Eigo Suyama; Renu Wadhwa; Kamaljit Kaur; Makoto Miyagishi; Sunil C Kaul; Hiroaki Kawasaki; Kazunari Taira
Identification of metastasis-related genes in a mouse model using a library of randomized ribozymes.
The Journal of biological chemistry 2004;279(37):38083-6.
2004: Hiroaki Kawasaki; Kazunari Taira
Induction of DNA methylation and gene silencing by short interfering RNAs in human cells.
Nature 2004;431(7005):211-7.
2004: Hiroaki Kawasaki; Renu Wadhwa; Kazunari Taira
World of small RNAs: from ribozymes to siRNA and miRNA.
Differentiation; research in biological diversity 2004;72(2-3):58-64.
2004: Eigo Suyama; Renu Wadhwa; Hiroaki Kawasaki; Tomoko Yaguchi; Sunil C Kaul; Motowo Nakajima; Kazunari Taira
LIM kinase-2 targeting as a possible anti-metastasis therapy.
The journal of gene medicine 2004;6(3):357-63.
2004: Reiko Onuki; Yoshio Bando; Eigo Suyama; Taiichi Katayama; Hiroaki Kawasaki; Tadashi Baba; Masaya Tohyama; Kazunari Taira
An RNA-dependent protein kinase is involved in tunicamycin-induced apoptosis and Alzheimer's disease.
The EMBO journal 2004;23(4):959-68.
2004: Hitomi Kimura; Hiroaki Kawasaki; Kazunari Taira
Mouse microRNA-23b regulates expression of Hes1 gene in P19 cells.
Nucleic acids symposium series (2004) 2004;(48):213-4.
2004: Hiroaki Kawasaki; Kazunari Taira
MicroRNA-196 inhibits HOXB8 expression in myeloid differentiation of HL60 cells.
Nucleic acids symposium series (2004) 2004;(48):211-2.
2004: Toshiyasu Mikuma; Hiroaki Kawasaki; Yasuhiko Yamamoto; Kazunari Taira
Overexpression of Dicer enhances RNAi-mediated gene silencing by short-hairpin RNAs (shRNAs) in human cells.
Nucleic acids symposium series (2004) 2004;(48):191-2.
2004: Eigo Suyama; Hiroaki Kawasaki; Renu Wadhwa; Kazunari Taira
Cell migration and metastasis as targets of small RNA-based molecular genetic analyses.
Journal of muscle research and cell motility 2004;25(4-5):303-8.
2004: Mayu Iyo; Hiroaki Kawasaki; Kazunari Taira
Maxizyme technology.
Methods in molecular biology (Clifton, N.J.) 2004;252():257-65.
2004: Yoshio Kato; Masaru Tsunemi; Makoto Miyagishi; Hiroaki Kawasaki; Kazunari Taira
Functional gene discovery using hybrid ribozyme libraries.
Methods in molecular biology (Clifton, N.J.) 2004;252():245-56.
2004: Hiroaki Kawasaki; Masaki Warashina; Tomoko Kuwabara; Kazunari Taira
Helicase-attached novel hybrid ribozymes.
Methods in molecular biology (Clifton, N.J.) 2004;252():237-43.
2003: Keisuke Oshima; Hiroaki Kawasaki; Yasushi Soda; Kenzaburo Tani; Shigetaka Asano; Kazunari Taira
Maxizymes and small hairpin-type RNAs that are driven by a tRNA promoter specifically cleave a chimeric gene associated with leukemia in vitro and in vivo.
Cancer research 2003;63(20):6809-14.
2003: Hiroaki Kawasaki; Kazunari Taira
Hes1 is a target of microRNA-23 during retinoic-acid-induced neuronal differentiation of NT2 cells.
Nature 2003;423(6942):838-42.
2003: Hiroshi Minoshima; Eigo Suyama; Hiroaki Kawasaki; Kazunari Taira
[RNAi-mediated specific gene silencing and its application to medical treatments of viral infectious disease].
Uirusu 2003;53(1):7-14.
2003: Renu Wadhwa; Hiroshi Ando; Hiroaki Kawasaki; Kazunari Taira; Sunil C Kaul
Targeting mortalin using conventional and RNA-helicase-coupled hammerhead ribozymes.
EMBO reports 2003;4(6):595-601.
2003: Eigo Suyama; Hiroaki Kawasaki; Motowo Nakajima; Kazunari Taira
Identification of genes involved in cell invasion by using a library of randomized hybrid ribozymes.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(10):5616-21.
2003: Reiko Onuki; Hiroaki Kawasaki; Tadashi Baba; Kazunari Taira
Analysis of a mitochondrial apoptotic pathway using Bid-targeted ribozymes in human MCF7 cells in the absence of a caspase-3-dependent pathway.
Antisense & nucleic acid drug development 2003;13(2):75-82.
2003: Eigo Suyama; Hiroaki Kawasaki; Reiko Onuki; Kazunari Taira
[Gene discovery system with randomized ribozyme libraries].
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 2003;48(4 Suppl):532-9.
2003: Hiroaki Kawasaki; Eigo Suyama; Mayu Iyo; Kazunari Taira
siRNAs generated by recombinant human Dicer induce specific and significant but target site-independent gene silencing in human cells.
Nucleic acids research 2003;31(3):981-7.
2003: Hiroaki Kawasaki; Kazunari Taira
Short hairpin type of dsRNAs that are controlled by tRNA(Val) promoter significantly induce RNAi-mediated gene silencing in the cytoplasm of human cells.
Nucleic acids research 2003;31(2):700-7.
2003: Hiroaki Kawasaki; Tomoko Kuwabara; Makoto Miyagishi; Kazunari Taira
Identification of functional genes by libraries of ribozymes and siRNAs.
Nucleic acids research. Supplement (2001) 2003;(3):331-2.
2003: Eigo Suyama; Hiroaki Kawasaki; Kazunari Taira
Use of a ribozyme library for validation of gene functions and cellular pathways.
Nucleic acids research. Supplement (2001) 2003;(3):253-4.
2003: Hiroshi Minoshima; Eigo Suyama; Hiroaki Kawasaki; Kazunari Taira
Cancer gene validation using ribozymes and macroarray.
Nucleic acids research. Supplement (2001) 2003;(3):251-2.
2003: Hiroaki Kawasaki; Kazunari Taira
Functional analysis of microRNAs during the retinoic acid-induced neuronal differentiation of human NT2 cells.
Nucleic acids research. Supplement (2001) 2003;(3):243-4.
2003: Eigo Suyama; Hiroaki Kawasaki; Tatsuhiko Kasaoka; Kazunari Taira
Identification of genes responsible for cell migration by a library of randomized ribozymes.
Cancer research 2003;63(1):119-24.
2002: Reiko Onuki; Akira Nagasaki; Hiroaki Kawasaki; Tadashi Baba; Taro Q P Uyeda; Kazunari Taira
Confirmation by FRET in individual living cells of the absence of significant amyloid beta -mediated caspase 8 activation.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(23):14716-21.
2002: Yasuomi Takagi; Eigo Suyama; Hiroaki Kawasaki; Makoto Miyagishi; Kazunari Taira
Mechanism of action of hammerhead ribozymes and their applications in vivo: rapid identification of functional genes in the post-genome era by novel hybrid ribozyme libraries.
Biochemical Society transactions 2002;30(Pt 6):1145-9.
2002: Eigo Suyama; Hiroaki Kawasaki; Kazunari Taira
Identification of a caspase 3-independent role of pro-apoptotic factor Bak in TNF-alpha-induced apoptosis.
FEBS letters 2002;528(1-3):63-9.
2002: Hiroaki Kawasaki; Kazunari Taira
A functional gene discovery in the Fas-mediated pathway to apoptosis by analysis of transiently expressed randomized hybrid-ribozyme libraries.
Nucleic acids research 2002;30(16):3609-14.
2002: Hideo Akashi; Hiroaki Kawasaki; Won Jong Kim; Toshihiro Akaike; Kazunari Taira; Atsushi Maruyama
Enhancement in the cleavage activity of a hammerhead ribozyme by cationic comb-type polymers and an RNA helicase in vitro.
Journal of biochemistry 2002;131(5):687-92.
2002: Hiroaki Kawasaki; Kazunari Taira
Identification of genes by hybrid ribozymes that couple cleavage activity with the unwinding activity of an endogenous RNA helicase.
EMBO reports 2002;3(5):443-50.
2002: Mayu Iyo; Hiroaki Kawasaki; Kazunari Taira
Allosterically controllable maxizymes for molecular gene therapy.
Current opinion in molecular therapeutics 2002;4(2):154-65.
2002: Hiroaki Kawasaki; Reiko Onuki; Eigo Suyama; Kazunari Taira
Identification of genes that function in the TNF-alpha-mediated apoptotic pathway using randomized hybrid ribozyme libraries.
Nature biotechnology 2002;20(4):376-80.
2002: Hiroaki Kawasaki; Masaru Tsunemi; Mayu Iyo; Keisuke Oshima; Hiroshi Minoshima; Akira Hamada; Reiko Onuki; Eigo Suyama; Kazunari Taira
A functional gene discovery in cell differentiation by hybrid ribozyme and siRNA libraries.
Nucleic acids research. Supplement (2001) 2002;(2):275-6.
2002: Eigo Suyama; Hiroshi Minoshima; Hiroaki Kawasaki; Kazunari Taira
Identification of AP-2-regulated genes by macroarray profiling of gene expression in human A375P melanoma.
Nucleic acids research. Supplement (2001) 2002;(2):247-8.
2002: Mayu Iyo; Hiroaki Kawasaki; Kazunari Taira
Construction of an allosteric trans-maxizyme targeting for two distinct oncogenes.
Nucleic acids research. Supplement (2001) 2002;(2):115-6.
2001: Eigo Suyama; Hiroaki Kawasaki; Kazunari Taira
Characterization of pro-apoptotic gene Bak using hammerhead ribozymes.
Nucleic acids research. Supplement (2001) 2001;(1):207-8.
2001: Hiroaki Kawasaki; Kazunari Taira
Discovery of functional genes in the post-genome era by novel RNA-protein hybrid ribozymes.
Nucleic acids research. Supplement (2001) 2001;(1):133-4.
2000: Tsuyoshi Tanabe; I Takata; Tomoko Kuwabara; Masaki Warashina; Hiroaki Kawasaki; Kenzaburo Tani; S Ohta; Shigetaka Asano; Kazunari Taira
Maxizymes, novel allosterically controllable ribozymes, can be designed to cleave various substrates.
Biomacromolecules 2000;1(1):108-17.
1999: T Kawakami; Toshiharu Suzuki; S H Baek; C H Chung; Hiroaki Kawasaki; H Hirano; A Ichiyama; M Omata; Keiichi Tanaka
Isolation and characterization of cytosolic and membrane-bound deubiquitinylating enzymes from bovine brain.
Journal of biochemistry 1999;126(3):612-23.