FIND

researchers

DISCOVER

relevant articles

PROMOTE

your research

now for free

Lewis Kay

Research Profile

Procedures

Nuclear Magnetic Resonance, Biomolecular

Concepts & Ideas

Phenomena

Chemicals & Drugs

Co-author Network

Publications

TOP 3
Pramodh Vallurupalli; Lewis E Kay
Probing slow chemical exchange at carbonyl sites in proteins by chemical exchange saturation transfer NMR spectroscopy.
Rina Rosenzweig; Arash Zarrine-Afsar; John R Glover; Shoeib Moradi; Lewis E Kay
Unraveling the mechanism of protein disaggregation through a ClpB-DnaK interaction.
Alexandar L Hansen; Guillaume Bouvignies; Lewis E Kay
Probing slowly exchanging protein systems via ¹³Cα-CEST: monitoring folding of the Im7 protein.

Register for free to view:

Visualized networks:

See your personal network in
sophisticated graphical views

GeoTargeted Searches:

Locate experts around the world
and connect with global collaborators

Research Profiles:

See the research activity
of experts around the globe

Geonetwork of Lewis Kay (preview)

Cities where this author has publications
Cities where co-authors of this author have publications

Flash Player Required

All Publications

2013: Pramodh Vallurupalli; Lewis E Kay
Probing slow chemical exchange at carbonyl sites in proteins by chemical exchange saturation transfer NMR spectroscopy.
Angewandte Chemie (International ed. in English) 2013;52(15):4156-9.
2013: Rina Rosenzweig; Arash Zarrine-Afsar; John R Glover; Shoeib Moradi; Lewis E Kay
Unraveling the mechanism of protein disaggregation through a ClpB-DnaK interaction.
Science (New York, N.Y.) 2013;339(6123):1080-3.
2013: Alexandar L Hansen; Guillaume Bouvignies; Lewis E Kay
Probing slowly exchanging protein systems via ¹³Cα-CEST: monitoring folding of the Im7 protein.
Journal of biomolecular NMR 2013;55(3):279-89.
2013: Michael P Latham; Lewis E Kay
Probing non-specific interactions of Ca²⁺-calmodulin in E. coli lysate.
Journal of biomolecular NMR 2013;55(3):239-47.
2013: Andrew Baldwin; Lewis E Kay
An R(1ρ) expression for a spin in chemical exchange between two sites with unequal transverse relaxation rates.
Journal of biomolecular NMR 2013;55(2):211-8.
2012: Guillaume Bouvignies; Lewis E Kay
Measurement of proton chemical shifts in invisible states of slowly exchanging protein systems by chemical exchange saturation transfer.
The journal of physical chemistry. B 2012;116(49):14311-7.
2012: Ashok Sekhar; Pramodh Vallurupalli; Lewis E Kay
Folding of the four-helix bundle FF domain from a compact on-pathway intermediate state is governed predominantly by water motion.
Proceedings of the National Academy of Sciences of the United States of America 2012;109(47):19268-73.
2012: Andrew Baldwin; Justin L P Benesch; D Flemming Hansen; Gillian R Hilton; Simon Sharpe; Patrick Walsh; Lewis E Kay
Probing dynamic conformations of the high-molecular-weight αB-crystallin heat shock protein ensemble by NMR spectroscopy.
Journal of the American Chemical Society 2012;134(37):15343-50.
2012: Andrew Baldwin; Lewis E Kay
Structural biology: Dynamic binding.
Nature 2012;488(7410):165-6.
2012: Guillaume Bouvignies; Lewis E Kay
A 2D ¹³C-CEST experiment for studying slowly exchanging protein systems using methyl probes: an application to protein folding.
Journal of biomolecular NMR 2012;53(4):303-10.
2012: Julia Barette; Dmitry M Korzhnev; Tomasz L Religa; Algirdas Velyvis; Lewis E Kay
Cross-validation of the structure of a transiently formed and low populated FF domain folding intermediate determined by relaxation dispersion NMR and CS-Rosetta.
The journal of physical chemistry. B 2012;116(23):6637-44.
2012: Pramodh Vallurupalli; Guillaume Bouvignies; Lewis E Kay
Studying "invisible" excited protein states in slow exchange with a major state conformation.
Journal of the American Chemical Society 2012;134(19):8148-61.
2012: Andrew Baldwin; Lewis E Kay
Measurement of the signs of methyl 13C chemical shift differences between interconverting ground and excited protein states by R(1ρ): an application to αB-crystallin.
Journal of biomolecular NMR 2012;53(1):1-12.
2012: Philipp Neudecker; Paul Robustelli; Simon Sharpe; Michele Vendruscolo; Patrick Walsh; Arash Zarrine-Afsar; Andrea Cavalli; Patrik Lundström; Lewis E Kay
Structure of an intermediate state in protein folding and aggregation.
Science (New York, N.Y.) 2012;336(6079):362-6.
2012: Alexandar L Hansen; Patrik Lundström; Algirdas Velyvis; Lewis E Kay
Quantifying millisecond exchange dynamics in proteins by CPMG relaxation dispersion NMR using side-chain 1H probes.
Journal of the American Chemical Society 2012;134(6):3178-89.
2012: Michael P Latham; Lewis E Kay
Is buffer a good proxy for a crowded cell-like environment? A comparative NMR study of calmodulin side-chain dynamics in buffer and E. coli lysate.
PloS one 2012;7(10):e48226.
2012: Algirdas Velyvis; Amy M Ruschak; Lewis E Kay
An economical method for production of (2)H, (13)CH3-threonine for solution NMR studies of large protein complexes: application to the 670 kDa proteasome.
PloS one 2012;7(9):e43725.
2011: Andrew Baldwin; Justin L P Benesch; Gillian R Hilton; Claire Bagnéris; Hadi Lioe; Lewis E Kay
Quaternary dynamics of αB-crystallin as a direct consequence of localised tertiary fluctuations in the C-terminus.
Journal of molecular biology 2011;413(2):310-20.
2011: Andrew Baldwin; Lewis E Kay; Hadi Lioe; Carol V Robinson; Justin L P Benesch
αB-crystallin polydispersity is a consequence of unbiased quaternary dynamics.
Journal of molecular biology 2011;413(2):297-309.
2011: Guillaume Bouvignies; Bruno E Correia; Frederick W Dahlquist; D Flemming Hansen; Alaji Bah; David Baker; oliver lange; Pramodh Vallurupalli; Robert M Vernon; Lewis E Kay
Solution structure of a minor and transiently formed state of a T4 lysozyme mutant.
Nature 2011;477(7362):111-4.
2011: Hidenori Kato; Lewis E Kay; Hugo van Ingen; Bing-Rui Zhou; Michael Bustin; Hanqiao Feng; Yawen Bai
Architecture of the high mobility group nucleosomal protein 2-nucleosome complex as revealed by methyl-based NMR.
Proceedings of the National Academy of Sciences of the United States of America 2011;108(30):12283-8.
2011: Dmitry M Korzhnev; Tomasz L Religa; Robert M Vernon; David Baker; Alan R Fersht; Alexandar L Hansen; Lewis E Kay
Nonnative interactions in the FF domain folding pathway from an atomic resolution structure of a sparsely populated intermediate: an NMR relaxation dispersion study.
Journal of the American Chemical Society 2011;133(28):10974-82.
2011: Tomasz L Religa; Rina Rosenzweig; Amy M Ruschak; Lewis E Kay
Site-directed methyl group labeling as an NMR probe of structure and dynamics in supramolecular protein systems: applications to the proteasome and to the ClpP protease.
Journal of the American Chemical Society 2011;133(23):9063-8.
2011: Lewis E Kay
Solution NMR spectroscopy of supra-molecular systems, why bother? A methyl-TROSY view.
Journal of magnetic resonance (San Diego, Calif. : 1997) 2011;210(2):159-70.
2011: Guillaume Bouvignies; Pramodh Vallurupalli; Matthew H J Cordes; D Flemming Hansen; Lewis E Kay
Measuring 1HN temperature coefficients in invisible protein states by relaxation dispersion NMR spectroscopy.
Journal of biomolecular NMR 2011;50(1):13-8.
2011: Madhusudan Dey; Algirdas Velyvis; John J Li; Elaine Chiu; David Chiovitti; Lewis E Kay; Frank Sicheri; Thomas E Dever
Requirement for kinase-induced conformational change in eukaryotic initiation factor 2alpha (eIF2alpha) restricts phosphorylation of Ser51.
Proceedings of the National Academy of Sciences of the United States of America 2011;108(11):4316-21.
2011: Guillaume Bouvignies; D Flemming Hansen; Pramodh Vallurupalli; Lewis E Kay
Divided-evolution-based pulse scheme for quantifying exchange processes in proteins: powerful complement to relaxation dispersion experiments.
Journal of the American Chemical Society 2011;133(6):1935-45.
2010: Amy M Ruschak; Algirdas Velyvis; Lewis E Kay
A simple strategy for ¹³C, ¹H labeling at the Ile-γ2 methyl position in highly deuterated proteins.
Journal of biomolecular NMR 2010;48(3):129-35.
2010: Amy M Ruschak; Tomasz L Religa; Sarah Breuer; Susanne Witt; Lewis E Kay
The proteasome antechamber maintains substrates in an unfolded state.
Nature 2010;467(7317):868-71.
2010: Dmitry M Korzhnev; Tomasz L Religa; Wiktor Banachewicz; Alan R Fersht; Lewis E Kay
A transient and low-populated protein-folding intermediate at atomic resolution.
Science (New York, N.Y.) 2010;329(5997):1312-6.
2010: Andrew Baldwin; Tomasz L Religa; D Flemming Hansen; Guillaume Bouvignies; Lewis E Kay
13CHD2 methyl group probes of millisecond time scale exchange in proteins by 1H relaxation dispersion: an application to proteasome gating residue dynamics.
Journal of the American Chemical Society 2010;132(32):10992-5.
2010: Xiaoming Li; Tabitha E Wood; Remco Sprangers; Gerrit Jansen; Niels E Franke; Xinliang Mao; Xiaoming Wang; Yi Zhang; Sue Ellen Verbrugge; Hans Adomat; Zhi Hua Li; Suzanne Trudel; Christine Chen; Tomasz L Religa; Nazir Jamal; Hans Messner; Jacqueline Cloos; David R Rose; Ami Navon; Emma Guns; Robert A Batey; Lewis E Kay; Aaron D Schimmer
Effect of noncompetitive proteasome inhibition on bortezomib resistance.
Journal of the National Cancer Institute 2010;102(14):1069-82.
2010: Tomasz L Religa; Lewis E Kay
Optimal methyl labeling for studies of supra-molecular systems.
Journal of biomolecular NMR 2010;47(3):163-9.
2010: D Flemming Hansen; Philipp Neudecker; Lewis E Kay
Determination of isoleucine side-chain conformations in ground and excited states of proteins from chemical shifts.
Journal of the American Chemical Society 2010;132(22):7589-91.
2010: Guillaume Bouvignies; Dmitry M Korzhnev; Philipp Neudecker; D Flemming Hansen; Matthew H J Cordes; Lewis E Kay
A simple method for measuring signs of (1)H (N) chemical shift differences between ground and excited protein states.
Journal of biomolecular NMR 2010;47(2):135-41.
2010: Tomasz L Religa; Remco Sprangers; Lewis E Kay
Dynamic regulation of archaeal proteasome gate opening as studied by TROSY NMR.
Science (New York, N.Y.) 2010;328(5974):98-102.
2010: Renate Auer; D Flemming Hansen; Philipp Neudecker; Dmitry M Korzhnev; D Ranjith Muhandiram; Robert Konrat; Lewis E Kay
Measurement of signs of chemical shift differences between ground and excited protein states: a comparison between H(S/M)QC and R1rho methods.
Journal of biomolecular NMR 2010;46(3):205-16.
2010: Tanja Mittag; Lewis E Kay; Julie D Forman-Kay
Protein dynamics and conformational disorder in molecular recognition.
Journal of molecular recognition : JMR 2010;23(2):105-16.
2010: D Flemming Hansen; Philipp Neudecker; Pramodh Vallurupalli; Frans A A Mulder; Lewis E Kay
Determination of Leu side-chain conformations in excited protein states by NMR relaxation dispersion.
Journal of the American Chemical Society 2010;132(1):42-3.
2010: Amy M Ruschak; Lewis E Kay
Methyl groups as probes of supra-molecular structure, dynamics and function.
Journal of biomolecular NMR 2010;46(1):75-87.
2009: Anthony K Mittermaier; Lewis E Kay
Observing biological dynamics at atomic resolution using NMR.
Trends in biochemical sciences 2009;34(12):601-11.
2009: Algirdas Velyvis; Howard K Schachman; Lewis E Kay
Assignment of Ile, Leu, and Val methyl correlations in supra-molecular systems: an application to aspartate transcarbamoylase.
Journal of the American Chemical Society 2009;131(45):16534-43.
2009: D Flemming Hansen; Haniqiao Feng; Zheng Zhou; Yawen Bai; Lewis E Kay
Selective characterization of microsecond motions in proteins by NMR relaxation.
Journal of the American Chemical Society 2009;131(44):16257-65.
2009: Andrew Baldwin; Lewis E Kay
NMR spectroscopy brings invisible protein states into focus.
Nature chemical biology 2009;5(11):808-14.
2009: James M Murphy; D Flemming Hansen; Silke Wiesner; D Ranjith Muhandiram; Mikael Borg; Matthew J Smith; Frank Sicheri; Lewis E Kay; Julie D Forman-Kay; Tony Pawson
Structural studies of FF domains of the transcription factor CA150 provide insights into the organization of FF domain tandem arrays.
Journal of molecular biology 2009;393(2):409-24.
2009: D Flemming Hansen; Pramodh Vallurupalli; Lewis E Kay
Measurement of methyl group motional parameters of invisible, excited protein states by NMR spectroscopy.
Journal of the American Chemical Society 2009;131(35):12745-54.
2009: Pramodh Vallurupalli; D Flemming Hansen; Patrik Lundström; Lewis E Kay
CPMG relaxation dispersion NMR experiments measuring glycine 1H alpha and 13C alpha chemical shifts in the 'invisible' excited states of proteins.
Journal of biomolecular NMR 2009;45(1-2):45-55.
2009: Andrew Baldwin; D Flemming Hansen; Pramodh Vallurupalli; Lewis E Kay
Measurement of methyl axis orientations in invisible, excited states of proteins by relaxation dispersion NMR spectroscopy.
Journal of the American Chemical Society 2009;131(33):11939-48.
2009: Renate Auer; Philipp Neudecker; D Ranjith Muhandiram; Patrik Lundström; D Flemming Hansen; Robert Konrat; Lewis E Kay
Measuring the signs of 1H(alpha) chemical shift differences between ground and excited protein states by off-resonance spin-lock R(1rho) NMR spectroscopy.
Journal of the American Chemical Society 2009;131(31):10832-3.
2009: Hugo van Ingen; Dmitry M Korzhnev; Lewis E Kay
An analysis of the effects of 1HN-(1)HN dipolar couplings on the measurement of amide bond vector orientations in invisible protein states by relaxation dispersion NMR.
The journal of physical chemistry. B 2009;113(29):9968-77.
2009: Algirdas Velyvis; Howard K Schachman; Lewis E Kay
Application of methyl-TROSY NMR to test allosteric models describing effects of nucleotide binding to aspartate transcarbamoylase.
Journal of molecular biology 2009;387(3):540-7.
2009: D Flemming Hansen; Zheng Zhou; Haniqiao Feng; Lisa M Miller Jenkins; Yawen Bai; Lewis E Kay
Binding kinetics of histone chaperone Chz1 and variant histone H2A.Z-H2B by relaxation dispersion NMR spectroscopy.
Journal of molecular biology 2009;387(1):1-9.
2009: Philipp Neudecker; Patrik Lundström; Lewis E Kay
Relaxation dispersion NMR spectroscopy as a tool for detailed studies of protein folding.
Biophysical journal 2009;96(6):2045-54.
2009: Dmitry M Korzhnev; Irina Bezsonova; Soyoung Lee; Tigran V Chalikian; Lewis E Kay
Alternate binding modes for a ubiquitin-SH3 domain interaction studied by NMR spectroscopy.
Journal of molecular biology 2009;386(2):391-405.
2009: Patrik Lundström; D Flemming Hansen; Pramodh Vallurupalli; Lewis E Kay
Accurate measurement of alpha proton chemical shifts of excited protein states by relaxation dispersion NMR spectroscopy.
Journal of the American Chemical Society 2009;131(5):1915-26.
2009: Patrik Lundström; Pramodh Vallurupalli; D Flemming Hansen; Lewis E Kay
Isotope labeling methods for studies of excited protein states by relaxation dispersion NMR spectroscopy.
Nature protocols 2009;4(11):1641-8.
2008: Tanja Mittag; Stephen Orlicky; Wing-Yiu Choy; Xiaojing Tang; Hong Lin; Frank Sicheri; Lewis E Kay; Mike Tyers; Julie D Forman-Kay
Dynamic equilibrium engagement of a polyvalent ligand with a single-site receptor.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(46):17772-7.
2008: Patrik Lundström; D Flemming Hansen; Lewis E Kay
Measurement of carbonyl chemical shifts of excited protein states by relaxation dispersion NMR spectroscopy: comparison between uniformly and selectively (13)C labeled samples.
Journal of biomolecular NMR 2008;42(1):35-47.
2008: Pramodh Vallurupalli; D Flemming Hansen; Lewis E Kay
Structures of invisible, excited protein states by relaxation dispersion NMR spectroscopy.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(33):11766-71.
2008: Zheng Zhou; Hanqiao Feng; D Flemming Hansen; Hidenori Kato; Ed Luk; Daron I Freedberg; Lewis E Kay; Carl Wu; Yawen Bai
NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B.
Nature structural & molecular biology 2008;15(8):868-9.
2008: Mathias Hass; D Flemming Hansen; Hans E M Christensen; Jens Led; Lewis E Kay
Characterization of conformational exchange of a histidine side chain: protonation, rotamerization, and tautomerization of His61 in plastocyanin from Anabaena variabilis.
Journal of the American Chemical Society 2008;130(26):8460-70.
2008: D Flemming Hansen; Pramodh Vallurupalli; Lewis E Kay
Quantifying two-bond 1HN-13CO and one-bond 1H(alpha)-13C(alpha) dipolar couplings of invisible protein states by spin-state selective relaxation dispersion NMR spectroscopy.
Journal of the American Chemical Society 2008;130(26):8397-405.
2008: D Flemming Hansen; Pramodh Vallurupalli; Lewis E Kay
Using relaxation dispersion NMR spectroscopy to determine structures of excited, invisible protein states.
Journal of biomolecular NMR 2008;41(3):113-20.
2008: Remco Sprangers; Xiaoming Li; Xinliang Mao; John L Rubinstein; Aaron D Schimmer; Lewis E Kay
TROSY-based NMR evidence for a novel class of 20S proteasome inhibitors.
Biochemistry 2008;47(26):6727-34.
2008: D Flemming Hansen; Pramodh Vallurupalli; Lewis E Kay
An improved 15N relaxation dispersion experiment for the measurement of millisecond time-scale dynamics in proteins.
The journal of physical chemistry. B 2008;112(19):5898-904.
2008: Pramodh Vallurupalli; D Flemming Hansen; Lewis E Kay
Probing structure in invisible protein states with anisotropic NMR chemical shifts.
Journal of the American Chemical Society 2008;130(9):2734-5.
2008: Dmitry M Korzhnev; Lewis E Kay
Probing invisible, low-populated States of protein molecules by relaxation dispersion NMR spectroscopy: an application to protein folding.
Accounts of chemical research 2008;41(3):442-51.
2008: D Flemming Hansen; Pramodh Vallurupalli; Patrik Lundström; Philipp Neudecker; Lewis E Kay
Probing chemical shifts of invisible states of proteins with relaxation dispersion NMR spectroscopy: how well can we do?
Journal of the American Chemical Society 2008;130(8):2667-75.
2007: Pramodh Vallurupalli; D Flemming Hansen; Elliott Stollar; Eva Meirovitch; Lewis E Kay
Measurement of bond vector orientations in invisible excited states of proteins.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(47):18473-7.
2007: Remco Sprangers; Lewis E Kay
Probing supramolecular structure from measurement of methyl (1)H-(13)C residual dipolar couplings.
Journal of the American Chemical Society 2007;129(42):12668-9.
2007: Philipp Neudecker; Arash Zarrine-Afsar; Alan R Davidson; Lewis E Kay
Phi-value analysis of a three-state protein folding pathway by NMR relaxation dispersion spectroscopy.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(40):15717-22.
2007: D Flemming Hansen; Daiwen Yang; Haniqiao Feng; Zheng Zhou; Silke Wiesner; Yawen Bai; Lewis E Kay
An exchange-free measure of 15N transverse relaxation: an NMR spectroscopy application to the study of a folding intermediate with pervasive chemical exchange.
Journal of the American Chemical Society 2007;129(37):11468-79.
2007: Dmitry M Korzhnev; Tomasz L Religa; Patrik Lundström; Alan Fersht; Lewis E Kay
The folding pathway of an FF domain: characterization of an on-pathway intermediate state under folding conditions by (15)N, (13)C(alpha) and (13)C-methyl relaxation dispersion and (1)H/(2)H-exchange NMR spectroscopy.
Journal of molecular biology 2007;372(2):497-512.
2007: James M Murphy; Dmitry M Korzhnev; Derek F J Ceccarelli; Douglas J Briant; Arash Zarrine-Afsar; Frank Sicheri; Lewis E Kay; Tony Pawson
Conformational instability of the MARK3 UBA domain compromises ubiquitin recognition and promotes interaction with the adjacent kinase domain.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(36):14336-41.
2007: Remco Sprangers; Algirdas Velyvis; Lewis E Kay
Solution NMR of supramolecular complexes: providing new insights into function.
Nature methods 2007;4(9):697-703.
2007: Vitali Tugarinov; Lewis E Kay
Separating degenerate (1)H transitions in methyl group probes for single-quantum (1)H-CPMG relaxation dispersion NMR spectroscopy.
Journal of the American Chemical Society 2007;129(30):9514-21.
2007: Patrik Lundström; Kaare Teilum; Tommy Carstensen; Irina Bezsonova; Silke Wiesner; D Flemming Hansen; Tomasz L Religa; Mikael Akke; Lewis E Kay
Fractional 13C enrichment of isolated carbons using [1-13C]- or [2- 13C]-glucose facilitates the accurate measurement of dynamics at backbone Calpha and side-chain methyl positions in proteins.
Journal of biomolecular NMR 2007;38(3):199-212.
2007: Algirdas Velyvis; Ying R Yang; Howard K Schachman; Lewis E Kay
A solution NMR study showing that active site ligands and nucleotides directly perturb the allosteric equilibrium in aspartate transcarbamoylase.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(21):8815-20.
2007: Patrik Lundström; Pramodh Vallurupalli; Tomasz L Religa; Frederick W Dahlquist; Lewis E Kay
A single-quantum methyl 13C-relaxation dispersion experiment with improved sensitivity.
Journal of biomolecular NMR 2007;38(1):79-88.
2007: Pramodh Vallurupalli; Lincoln Scott; James R Williamson; Lewis E Kay
Strong coupling effects during X-pulse CPMG experiments recorded on heteronuclear ABX spin systems: artifacts and a simple solution.
Journal of biomolecular NMR 2007;38(1):41-6.
2007: D Flemming Hansen; Lewis E Kay
Improved magnetization alignment schemes for spin-lock relaxation experiments.
Journal of biomolecular NMR 2007;37(4):245-55.
2007: Vitali Tugarinov; Remco Sprangers; Lewis E Kay
Probing side-chain dynamics in the proteasome by relaxation violated coherence transfer NMR spectroscopy.
Journal of the American Chemical Society 2007;129(6):1743-50.
2007: Remco Sprangers; Lewis E Kay
Quantitative dynamics and binding studies of the 20S proteasome by NMR.
Nature 2007;445(7128):618-22.
2006: Philipp Neudecker; Arash Zarrine-Afsar; Wing-Yiu Choy; D Ranjith Muhandiram; Alan R Davidson; Lewis E Kay
Identification of a collapsed intermediate with non-native long-range interactions on the folding pathway of a pair of Fyn SH3 domain mutants by NMR relaxation dispersion spectroscopy.
Journal of molecular biology 2006;363(5):958-76.
2006: Vitali Tugarinov; Lewis E Kay
A 2H NMR relaxation experiment for the measurement of the time scale of methyl side-chain dynamics in large proteins.
Journal of the American Chemical Society 2006;128(38):12484-9.
2006: Dmitry M Korzhnev; Philipp Neudecker; Arash Zarrine-Afsar; Alan R Davidson; Lewis E Kay
Abp1p and Fyn SH3 domains fold through similar low-populated intermediate states.
Biochemistry 2006;45(34):10175-83.
2006: Pramodh Vallurupalli; Lewis E Kay
Complementarity of ensemble and single-molecule measures of protein motion: a relaxation dispersion NMR study of an enzyme complex.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(32):11910-5.
2006: Pramodh Vallurupalli; Lincoln Scott; Mirko Hennig; James R Williamson; Lewis E Kay
New RNA labeling methods offer dramatic sensitivity enhancements in 2H NMR relaxation spectra.
Journal of the American Chemical Society 2006;128(29):9346-7.
2006: Vitali Tugarinov; Jason E Ollerenshaw; Lewis E Kay
Dipolar dynamic frequency shifts in multiple-quantum spectra of methyl groups in proteins: correlation with side-chain motion.
Magnetic resonance in chemistry : MRC 2006;44 Spec No():S122-9.
2006: Evanics Ferenc; Peter M Hwang; Yao Cheng; Lewis E Kay; R Scott Prosser
Topology of an outer-membrane enzyme: Measuring oxygen and water contacts in solution NMR studies of PagP.
Journal of the American Chemical Society 2006;128(25):8256-64.
2006: Vitali Tugarinov; Lewis E Kay
Relaxation rates of degenerate 1H transitions in methyl groups of proteins as reporters of side-chain dynamics.
Journal of the American Chemical Society 2006;128(22):7299-308.
2006: Martin Tollinger; Chris Neale; Lewis E Kay; Julie D Forman-Kay
Characterization of the hydrodynamic properties of the folding transition state of an SH3 domain by magnetization transfer NMR spectroscopy.
Biochemistry 2006;45(20):6434-45.
2006: Ulrika Brath; Mikael Akke; Daiwen Yang; Lewis E Kay; Frans A A Mulder
Functional dynamics of human FKBP12 revealed by methyl 13C rotating frame relaxation dispersion NMR spectroscopy.
Journal of the American Chemical Society 2006;128(17):5718-27.
2006: Dmitry M Korzhnev; Irina Bezsonova; Evanics Ferenc; Nicolas Taulier; Zheng Zhou; Yawen Bai; Tigran V Chalikian; R Scott Prosser; Lewis E Kay
Probing the transition state ensemble of a protein folding reaction by pressure-dependent NMR relaxation dispersion.
Journal of the American Chemical Society 2006;128(15):5262-9.
2006: Irina Bezsonova; Dmitry M Korzhnev; R Scott Prosser; Julie D Forman-Kay; Lewis E Kay
Hydration and packing along the folding pathway of SH3 domains by pressure-dependent NMR.
Biochemistry 2006;45(15):4711-9.
2006: Anthony Mittermaier; Lewis E Kay
New tools provide new insights in NMR studies of protein dynamics.
Science (New York, N.Y.) 2006;312(5771):224-8.
2006: Philipp Neudecker; Dmitry M Korzhnev; Lewis E Kay
Assessment of the effects of increased relaxation dispersion data on the extraction of 3-site exchange parameters characterizing the unfolding of an SH3 domain.
Journal of biomolecular NMR 2006;34(3):129-35.
2006: Vitali Tugarinov; Voula Kanelis; Lewis E Kay
Isotope labeling strategies for the study of high-molecular-weight proteins by solution NMR spectroscopy.
Nature protocols 2006;1(2):749-54.
2006: Arash Zarrine-Afsar; Anthony Mittermaier; Lewis E Kay; Alan R Davidson
Protein stabilization by specific binding of guanidinium to a functional arginine-binding surface on an SH3 domain.
Protein science : a publication of the Protein Society 2006;15(1):162-70.
2005: Vitali Tugarinov; Lewis E Kay
Quantitative 13C and 2H NMR relaxation studies of the 723-residue enzyme malate synthase G reveal a dynamic binding interface.
Biochemistry 2005;44(49):15970-7.
2005: Anthony Mittermaier; Dmitry M Korzhnev; Lewis E Kay
Side-chain interactions in the folding pathway of a Fyn SH3 domain mutant studied by relaxation dispersion NMR spectroscopy.
Biochemistry 2005;44(47):15430-6.
2005: Remco Sprangers; Anna Gribun; Peter M Hwang; Walid A Houry; Lewis E Kay
Quantitative NMR spectroscopy of supramolecular complexes: dynamic side pores in ClpP are important for product release.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(46):16678-83.
2005: Dmitry M Korzhnev; Philipp Neudecker; Anthony Mittermaier; Vladislav Yu Orekhov; Lewis E Kay
Multiple-site exchange in proteins studied with a suite of six NMR relaxation dispersion experiments: an application to the folding of a Fyn SH3 domain mutant.
Journal of the American Chemical Society 2005;127(44):15602-11.
2005: Elan Z Eisenmesser; Oscar Millet; Wladimir Labeikovsky; Dmitry M Korzhnev; Magnus Wolf-Watz; Daryl A Bosco; Jack J Skalicky; Lewis E Kay; Dorothee Kern
Intrinsic dynamics of an enzyme underlies catalysis.
Nature 2005;438(7064):117-21.
2005: Jason E Ollerenshaw; Vitali Tugarinov; Nikolai R Skrynnikov; Lewis E Kay
Comparison of 13CH3, 13CH2D, and 13CHD2 methyl labeling strategies in proteins.
Journal of biomolecular NMR 2005;33(1):25-41.
2005: Vitali Tugarinov; Lewis E Kay
Methyl groups as probes of structure and dynamics in NMR studies of high-molecular-weight proteins.
Chembiochem : a European journal of chemical biology 2005;6(9):1567-77.
2005: Miles A Pufall; Gregory M Lee; Mary L Nelson; Hyun-Seo Kang; Algirdas Velyvis; Lewis E Kay; Lawrence P McIntosh; Barbara J Graves
Variable control of Ets-1 DNA binding by multiple phosphates in an unstructured region.
Science (New York, N.Y.) 2005;309(5731):142-5.
2005: Martin Tollinger; Lewis E Kay; Julie D Forman-Kay
Measuring pK(a) values in protein folding transition state ensembles by NMR spectroscopy.
Journal of the American Chemical Society 2005;127(25):8904-5.
2005: Vitali Tugarinov; Jason E Ollerenshaw; Lewis E Kay
Probing side-chain dynamics in high molecular weight proteins by deuterium NMR spin relaxation: an application to an 82-kDa enzyme.
Journal of the American Chemical Society 2005;127(22):8214-25.
2005: Pramodh Vallurupalli; Lewis E Kay
A suite of 2H NMR spin relaxation experiments for the measurement of RNA dynamics.
Journal of the American Chemical Society 2005;127(18):6893-901.
2005: Wing-Yiu Choy; Zheng Zhou; Yawen Bai; Lewis E Kay
An 15N NMR spin relaxation dispersion study of the folding of a pair of engineered mutants of apocytochrome b562.
Journal of the American Chemical Society 2005;127(14):5066-72.
2005: Dmitry M Korzhnev; Anthony Mittermaier; Lewis E Kay
Cross-correlated spin relaxation effects in methyl 1H CPMG-based relaxation dispersion experiments: complications and a simple solution.
Journal of biomolecular NMR 2005;31(4):337-42.
2005: Lewis E Kay
NMR studies of protein structure and dynamics.
Journal of magnetic resonance (San Diego, Calif. : 1997) 2005;173(2):193-207.
2005: Jun Xu; Oscar Millet; Lewis E Kay; Nikolai R Skrynnikov
A new spin probe of protein dynamics: nitrogen relaxation in 15N-2H amide groups.
Journal of the American Chemical Society 2005;127(9):3220-9.
2005: Vitali Tugarinov; Lewis E Kay; Ilghiz Ibraghimov; Vladislav Yu Orekhov
High-resolution four-dimensional 1H-13C NOE spectroscopy using methyl-TROSY, sparse data acquisition, and multidimensional decomposition.
Journal of the American Chemical Society 2005;127(8):2767-75.
2005: Dmitry M Korzhnev; Vladislav Yu Orekhov; Lewis E Kay
Off-resonance R(1rho) NMR studies of exchange dynamics in proteins with low spin-lock fields: an application to a Fyn SH3 domain.
Journal of the American Chemical Society 2005;127(2):713-21.
2005: Patrick J Finerty; Anthony Mittermaier; Ranjith Muhandiram; Lewis E Kay; Julie D Forman-Kay
NMR dynamics-derived insights into the binding properties of a peptide interacting with an SH2 domain.
Biochemistry 2005;44(2):694-703.
2005: Vitali Tugarinov; Wing-Yiu Choy; Vladislav Yu Orekhov; Lewis E Kay
Solution NMR-derived global fold of a monomeric 82-kDa enzyme.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(3):622-7.
2005: Peter M Hwang; Lewis E Kay
Solution structure and dynamics of integral membrane proteins by NMR: a case study involving the enzyme PagP.
Methods in enzymology 2005;394():335-50.
2004: Vitali Tugarinov; Christoph Scheurer; Rafael Brüschweiler; Lewis E Kay
Estimates of methyl 13C and 1H CSA values (Deltasigma) in proteins from cross-correlated spin relaxation.
Journal of biomolecular NMR 2004;30(4):397-406.
2004: Vitali Tugarinov; Wing-Yiu Choy; Eriks Kupce; Lewis E Kay
Addressing the overlap problem in the quantitative analysis of two dimensional NMR spectra: application to (15)N relaxation measurements.
Journal of biomolecular NMR 2004;30(3):347-52.
2004: Jason E Ollerenshaw; Hüseyin Kaya; Hue Sun Chan; Lewis E Kay
Sparsely populated folding intermediates of the Fyn SH3 domain: matching native-centric essential dynamics and experiment.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(41):14748-53.
2004: Vitali Tugarinov; Lewis E Kay
Stereospecific NMR assignments of prochiral methyls, rotameric states and dynamics of valine residues in malate synthase G.
Journal of the American Chemical Society 2004;126(31):9827-36.
2004: Victoria E Ahn; Eileen I Lo; Christian K Engel; Lu Chen; Peter M Hwang; Lewis E Kay; Russell E Bishop; Gilbert G Privé
A hydrocarbon ruler measures palmitate in the enzymatic acylation of endotoxin.
The EMBO journal 2004;23(15):2931-41.
2004: Dmitry M Korzhnev; Xavier Salvatella; Michele Vendruscolo; Ariel A Di Nardo; Alan R Davidson; Christopher M Dobson; Lewis E Kay
Low-populated folding intermediates of Fyn SH3 characterized by relaxation dispersion NMR.
Nature 2004;430(6999):586-90.
2004: Vitali Tugarinov; Lewis E Kay
1H,13C-1H,1H dipolar cross-correlated spin relaxation in methyl groups.
Journal of biomolecular NMR 2004;29(3):369-76.
2004: Peter M Hwang; Russell E Bishop; Lewis E Kay
The integral membrane enzyme PagP alternates between two dynamically distinct states.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(26):9618-23.
2004: Dmitry M Korzhnev; Karin Kloiber; Lewis E Kay
Multiple-quantum relaxation dispersion NMR spectroscopy probing millisecond time-scale dynamics in proteins: theory and application.
Journal of the American Chemical Society 2004;126(23):7320-9.
2004: Ariel A Di Nardo; Dmitry M Korzhnev; Peter J Stogios; Arash Zarrine-Afsar; Lewis E Kay; Alan R Davidson
Dramatic acceleration of protein folding by stabilization of a nonnative backbone conformation.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(21):7954-9.
2004: Vitali Tugarinov; Remco Sprangers; Lewis E Kay
Line narrowing in methyl-TROSY using zero-quantum 1H-13C NMR spectroscopy.
Journal of the American Chemical Society 2004;126(15):4921-5.
2004: Anthony Mittermaier; Lewis E Kay
The response of internal dynamics to hydrophobic core mutations in the SH3 domain from the Fyn tyrosine kinase.
Protein science : a publication of the Protein Society 2004;13(4):1088-99.
2004: Dmitry M Korzhnev; Karin Kloiber; Voula Kanelis; Vitali Tugarinov; Lewis E Kay
Probing slow dynamics in high molecular weight proteins by methyl-TROSY NMR spectroscopy: application to a 723-residue enzyme.
Journal of the American Chemical Society 2004;126(12):3964-73.
2004: Vitali Tugarinov; Lewis E Kay
An isotope labeling strategy for methyl TROSY spectroscopy.
Journal of biomolecular NMR 2004;28(2):165-72.
2004: Vitali Tugarinov; Peter M Hwang; Lewis E Kay
Nuclear magnetic resonance spectroscopy of high-molecular-weight proteins.
Annual review of biochemistry 2004;73():107-46.
2003: Jason E Ollerenshaw; Daniel A Lidar; Lewis E Kay
Magnetic resonance realization of decoherence-free quantum computation.
Physical review letters 2003;91(21):217904.
2003: Vitali Tugarinov; Lewis E Kay
Ile, Leu, and Val methyl assignments of the 723-residue malate synthase G using a new labeling strategy and novel NMR methods.
Journal of the American Chemical Society 2003;125(45):13868-78.
2003: Oscar Millet; Rhea P Hudson; Lewis E Kay
The energetic cost of domain reorientation in maltose-binding protein as studied by NMR and fluorescence spectroscopy.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(22):12700-5.
2003: Wing-Yiu Choy; Lewis E Kay
Probing residual interactions in unfolded protein states using NMR spin relaxation techniques: an application to delta131delta.
Journal of the American Chemical Society 2003;125(39):11988-92.
2003: Vitali Tugarinov; Peter M Hwang; Jason E Ollerenshaw; Lewis E Kay
Cross-correlated relaxation enhanced 1H[bond]13C NMR spectroscopy of methyl groups in very high molecular weight proteins and protein complexes.
Journal of the American Chemical Society 2003;125(34):10420-8.
2003: Anthony Mittermaier; Alan R Davidson; Lewis E Kay
Correlation between 2H NMR side-chain order parameters and sequence conservation in globular proteins.
Journal of the American Chemical Society 2003;125(30):9004-5.
2003: Oscar Millet; Anthony Mittermaier; David Baker; Lewis E Kay
The effects of mutations on motions of side-chains in protein L studied by 2H NMR dynamics and scalar couplings.
Journal of molecular biology 2003;329(3):551-63.
2003: Vitali Tugarinov; Lewis E Kay
Side chain assignments of Ile delta 1 methyl groups in high molecular weight proteins: an application to a 46 ns tumbling molecule.
Journal of the American Chemical Society 2003;125(19):5701-6.
2003: Dmitry M Korzhnev; Vladislav Yu Orekhov; Frederick W Dahlquist; Lewis E Kay
Off-resonance R1rho relaxation outside of the fast exchange limit: an experimental study of a cavity mutant of T4 lysozyme.
Journal of biomolecular NMR 2003;26(1):39-48.
2003: Martin Tollinger; Karin A Crowhurst; Lewis E Kay; Julie D Forman-Kay
Site-specific contributions to the pH dependence of protein stability.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(8):4545-50.
2003: Vitali Tugarinov; Lewis E Kay
Quantitative NMR studies of high molecular weight proteins: application to domain orientation and ligand binding in the 723 residue enzyme malate synthase G.
Journal of molecular biology 2003;327(5):1121-33.
2003: Wing-Yiu Choy; Lewis E Kay
Model selection for the interpretation of protein side chain methyl dynamics.
Journal of biomolecular NMR 2003;25(4):325-33.
2003: Wing-Yiu Choy; David Shortle; Lewis E Kay
Side chain dynamics in unfolded protein states: an NMR based 2H spin relaxation study of delta131delta.
Journal of the American Chemical Society 2003;125(7):1748-58.
2002: Tapas Mal; Mitsuhiko Ikura; Lewis E Kay
The ATCUN domain as a probe of intermolecular interactions: application to calmodulin-peptide complexes.
Journal of the American Chemical Society 2002;124(47):14002-3.
2002: Tapas Mal; Nikolai R Skrynnikov; Kyoko L Yap; Lewis E Kay; Mitsuhiko Ikura
Detecting protein kinase recognition modes of calmodulin by residual dipolar couplings in solution NMR.
Biochemistry 2002;41(43):12899-906.
2002: Logan W Donaldson; Gerald Gish; Tony Pawson; Lewis E Kay; Julie D Forman-Kay
Structure of a regulatory complex involving the Abl SH3 domain, the Crk SH2 domain, and a Crk-derived phosphopeptide.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(22):14053-8.
2002: Nikolai R Skrynnikov; Frederick W Dahlquist; Lewis E Kay
Reconstructing NMR spectra of "invisible" excited protein states using HSQC and HMQC experiments.
Journal of the American Chemical Society 2002;124(41):12352-60.
2002: Peter M Hwang; Wing-Yiu Choy; Eileen I Lo; Lu Chen; Julie D Forman-Kay; Christian R H Raetz; Gilbert G Privé; Russell E Bishop; Lewis E Kay
Solution structure and dynamics of the outer membrane enzyme PagP by NMR.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(21):13560-5.
2002: Dmitry M Korzhnev; Nikolai R Skrynnikov; Oscar Millet; Dennis A Torchia; Lewis E Kay
An NMR experiment for the accurate measurement of heteronuclear spin-lock relaxation rates.
Journal of the American Chemical Society 2002;124(36):10743-53.
2002: Vitali Tugarinov; Ranjith Muhandiram; Ayeda Ayed; Lewis E Kay
Four-dimensional NMR spectroscopy of a 723-residue protein: chemical shift assignments and secondary structure of malate synthase g.
Journal of the American Chemical Society 2002;124(34):10025-35.
2002: Nikolai R Skrynnikov; Oscar Millet; Lewis E Kay
Deuterium spin probes of side-chain dynamics in proteins. 2. Spectral density mapping and identification of nanosecond time-scale side-chain motions.
Journal of the American Chemical Society 2002;124(22):6449-60.
2002: Oscar Millet; D Ranjith Muhandiram; Nikolai R Skrynnikov; Lewis E Kay
Deuterium spin probes of side-chain dynamics in proteins. 1. Measurement of five relaxation rates per deuteron in (13)C-labeled and fractionally (2)H-enriched proteins in solution.
Journal of the American Chemical Society 2002;124(22):6439-48.
2002: Martin Tollinger; Julie D Forman-Kay; Lewis E Kay
Measurement of side-chain carboxyl pK(a) values of glutamate and aspartate residues in an unfolded protein by multinuclear NMR spectroscopy.
Journal of the American Chemical Society 2002;124(20):5714-7.
2002: Anthony Mittermaier; Lewis E Kay
Effect of deuteration on some structural parameters of methyl groups in proteins as evaluated by residual dipolar couplings.
Journal of biomolecular NMR 2002;23(1):35-45.
2002: Frans A A Mulder; Bin Hon; Anthony Mittermaier; Frederick W Dahlquist; Lewis E Kay
Slow internal dynamics in proteins: application of NMR relaxation dispersion spectroscopy to methyl groups in a cavity mutant of T4 lysozyme.
Journal of the American Chemical Society 2002;124(7):1443-51.
2002: Wing-Yiu Choy; Frans A A Mulder; Karin A Crowhurst; D Ranjith Muhandiram; Ian S Millett; Sebastian Doniach; Julie D Forman-Kay; Lewis E Kay
Distribution of molecular size within an unfolded state ensemble using small-angle X-ray scattering and pulse field gradient NMR techniques.
Journal of molecular biology 2002;316(1):101-12.
2002: Peter M Hwang; Chengjun Li; Massimo Morra; Jennifer Lillywhite; D Ranjith Muhandiram; Frank Gertler; Cox Terhorst; Lewis E Kay; Tony Pawson; Julie D Forman-Kay; Shun-Cheng Li
A "three-pronged" binding mechanism for the SAP/SH2D1A SH2 domain: structural basis and relevance to the XLP syndrome.
The EMBO journal 2002;21(3):314-23.
2001: Voula Kanelis; Julie D Forman-Kay; Lewis E Kay
Multidimensional NMR methods for protein structure determination.
IUBMB life 2001;52(6):291-302.
2001: Martin Tollinger; Nikolai R Skrynnikov; Frans A A Mulder; Julie D Forman-Kay; Lewis E Kay
Slow dynamics in folded and unfolded states of an SH3 domain.
Journal of the American Chemical Society 2001;123(46):11341-52.
2001: Frans A A Mulder; Anthony Mittermaier; Bin Hon; Frederick W Dahlquist; Lewis E Kay
Studying excited states of proteins by NMR spectroscopy.
Nature structural biology 2001;8(11):932-5.
2001: Logan W Donaldson; Nikolai R Skrynnikov; Wing-Yiu Choy; D Ranjith Muhandiram; B Sarkar; Julie D Forman-Kay; Lewis E Kay
Structural characterization of proteins with an attached ATCUN motif by paramagnetic relaxation enhancement NMR spectroscopy.
Journal of the American Chemical Society 2001;123(40):9843-7.
2001: Wing-Yiu Choy; Martin Tollinger; Geoffrey A Mueller; Lewis E Kay
Direct structure refinement of high molecular weight proteins against residual dipolar couplings and carbonyl chemical shift changes upon alignment: an application to maltose binding protein.
Journal of biomolecular NMR 2001;21(1):31-40.
2001: Ayeda Ayed; Frans A A Mulder; Gwan-Su Yi; Ying Lu; Lewis E Kay; Cheryl H Arrowsmith
Latent and active p53 are identical in conformation.
Nature structural biology 2001;8(9):756-60.
2001: Anthony Mittermaier; Lewis E Kay
Chi1 torsion angle dynamics in proteins from dipolar couplings.
Journal of the American Chemical Society 2001;123(28):6892-903.
2001: J Evenäs; Vitali Tugarinov; Nikolai R Skrynnikov; NK Goto; Ranjith Muhandiram; Lewis E Kay
Ligand-induced structural changes to maltodextrin-binding protein as studied by solution NMR spectroscopy.
Journal of molecular biology 2001;309(4):961-74.
2001: Nikolai R Skrynnikov; Frans A A Mulder; Bin Hon; Frederick W Dahlquist; Lewis E Kay
Probing slow time scale dynamics at methyl-containing side chains in proteins by relaxation dispersion NMR measurements: application to methionine residues in a cavity mutant of T4 lysozyme.
Journal of the American Chemical Society 2001;123(19):4556-66.
2001: NK Goto; Nikolai R Skrynnikov; Frederick W Dahlquist; Lewis E Kay
What is the average conformation of bacteriophage T4 lysozyme in solution? A domain orientation study using dipolar couplings measured by solution NMR.
Journal of molecular biology 2001;308(4):745-64.
2001: Peter M Hwang; Nikolai R Skrynnikov; Lewis E Kay
Domain orientation in beta-cyclodextrin-loaded maltose binding protein: diffusion anisotropy measurements confirm the results of a dipolar coupling study.
Journal of biomolecular NMR 2001;20(1):83-8.
2001: J Evenäs; Anthony Mittermaier; Dingqiao Yang; Lewis E Kay
Measurement of (13)C(alpha)-(13)C(beta) dipolar couplings in (15)N,(13)C,(2)H-labeled proteins: application to domain orientation in maltose binding protein.
Journal of the American Chemical Society 2001;123(12):2858-64.
2001: JR Tolman; Hashim M Al-Hashimi; Lewis E Kay; James H Prestegard
Structural and dynamic analysis of residual dipolar coupling data for proteins.
Journal of the American Chemical Society 2001;123(7):1416-24.
2001: Frans A A Mulder; Nikolai R Skrynnikov; Bin Hon; Frederick W Dahlquist; Lewis E Kay
Measurement of slow (micros-ms) time scale dynamics in protein side chains by (15)N relaxation dispersion NMR spectroscopy: application to Asn and Gln residues in a cavity mutant of T4 lysozyme.
Journal of the American Chemical Society 2001;123(5):967-75.
2001: Lewis E Kay
Nuclear magnetic resonance methods for high molecular weight proteins: a study involving a complex of maltose binding protein and beta-cyclodextrin.
Methods in enzymology 2001;339():174-203.
2000: Nikolai R Skrynnikov; Lewis E Kay
Assessment of molecular structure using frame-independent orientational restraints derived from residual dipolar couplings.
Journal of biomolecular NMR 2000;18(3):239-52.
2000: Geoffrey A Mueller; Wing-Yiu Choy; Nikolai R Skrynnikov; Lewis E Kay
A method for incorporating dipolar couplings into structure calculations in cases of (near) axial symmetry of alignment.
Journal of biomolecular NMR 2000;18(3):183-8.
2000: Frans A A Mulder; Bin Hon; D Ranjith Muhandiram; Frederick W Dahlquist; Lewis E Kay
Flexibility and ligand exchange in a buried cavity mutant of T4 lysozyme studied by multinuclear NMR.
Biochemistry 2000;39(41):12614-22.
2000: Frans A A Mulder; Ayeda Ayed; D Yang; Cheryl H Arrowsmith; Lewis E Kay
Assignment of 1H(N), 15N, 13C(alpha), 13CO and 13C(beta) resonances in a 67 kDa p53 dimer using 4D-TROSY NMR spectroscopy.
Journal of biomolecular NMR 2000;18(2):173-6.
2000: N K Goto; Lewis E Kay
New developments in isotope labeling strategies for protein solution NMR spectroscopy.
Current opinion in structural biology 2000;10(5):585-92.
2000: Geoffrey A Mueller; Wing-Yiu Choy; Dingqiao Yang; Julie D Forman-Kay; Ronald A Venters; Lewis E Kay
Global folds of proteins with low densities of NOEs using residual dipolar couplings: application to the 370-residue maltodextrin-binding protein.
Journal of molecular biology 2000;300(1):197-212.
2000: Catherine Zwahlen; SC Li; Lewis E Kay; Tony Pawson; Julie D Forman-Kay
Multiple modes of peptide recognition by the PTB domain of the cell fate determinant Numb.
The EMBO journal 2000;19(7):1505-15.
2000: test testing; SI Gunderson; Iain W Mattaj; Lewis E Kay; David Neuhaus; Gabriele Varani
The NMR structure of the 38 kDa U1A protein - PIE RNA complex reveals the basis of cooperativity in regulation of polyadenylation by human U1A protein.
Nature structural biology 2000;7(4):329-35.
2000: Voula Kanelis; L Donaldson; D Ranjith Muhandiram; Daniela Rotin; Julie D Forman-Kay; Lewis E Kay
Sequential assignment of proline-rich regions in proteins: application to modular binding domain complexes.
Journal of biomolecular NMR 2000;16(3):253-9.
2000: Nikolai R Skrynnikov; NK Goto; Dingqiao Yang; Wing-Yiu Choy; JR Tolman; Geoffrey A Mueller; Lewis E Kay
Orienting domains in proteins using dipolar couplings measured by liquid-state NMR: differences in solution and crystal forms of maltodextrin binding protein loaded with beta-cyclodextrin.
Journal of molecular biology 2000;295(5):1265-73.
1999: Anthony Mittermaier; test testing; D Ranjith Muhandiram; Lewis E Kay; Gabriele Varani
Changes in side-chain and backbone dynamics identify determinants of specificity in RNA recognition by human U1A protein.
Journal of molecular biology 1999;294(4):967-79.
1999: S C Li; Gerald Gish; D Yang; A J Coffey; Julie D Forman-Kay; Ingemar Ernberg; Lewis E Kay; Tony Pawson
Novel mode of ligand binding by the SH2 domain of the human XLP disease gene product SAP/SH2D1A.
Current biology : CB 1999;9(23):1355-62.
1999: NK Goto; KH Gardner; Geoffrey A Mueller; R C Willis; Lewis E Kay
A robust and cost-effective method for the production of Val, Leu, Ile (delta 1) methyl-protonated 15N-, 13C-, 2H-labeled proteins.
Journal of biomolecular NMR 1999;13(4):369-74.
1999: Anthony Mittermaier; Lewis E Kay; Julie D Forman-Kay
Analysis of deuterium relaxation-derived methyl axis order parameters and correlation with local structure.
Journal of biomolecular NMR 1999;13(2):181-5.
1999: Dingqiao Yang; Lewis E Kay
Improved 1HN-detected triple resonance TROSY-based experiments.
Journal of biomolecular NMR 1999;13(1):3-10.
1998: SC Li; Catherine Zwahlen; Sébastien J F Vincent; C J McGlade; Lewis E Kay; Tony Pawson; Julie D Forman-Kay
Structure of a Numb PTB domain-peptide complex suggests a basis for diverse binding specificity.
Nature structural biology 1998;5(12):1075-83.
1998: D Liu; R Ishima; Kit I Tong; Stefan Bagby; Tetsuro Kokubo; D Ranjith Muhandiram; Lewis E Kay; Yoshihiro Nakatani; Mitsuhiko Ikura
Solution structure of a TBP-TAF(II)230 complex: protein mimicry of the minor groove surface of the TATA box unwound by TBP.
Cell 1998;94(5):573-83.
1998: Dingqiao Yang; J R Tolman; NK Goto; Lewis E Kay
An HNCO-based Pulse Scheme for the Measurement of 13Cα-1Hα One-bond Dipolar couplings in 15N, 13C Labeled Proteins.
Journal of biomolecular NMR 1998;12(2):325-32.
1998: Lewis E Kay
Protein dynamics from NMR.
Nature structural biology 1998;5 Suppl():513-7.
1998: Pascale Legault; Joyce Li; Jeremy Mogridge; Lewis E Kay; Jack Greenblatt
NMR structure of the bacteriophage lambda N peptide/boxB RNA complex: recognition of a GNRA fold by an arginine-rich motif.
Cell 1998;93(2):289-99.
1998: X Shan; KH Gardner; D Ranjith Muhandiram; Lewis E Kay; Cheryl H Arrowsmith
Subunit-specific backbone NMR assignments of a 64 kDa trp repressor/DNA complex: a role for N-terminal residues in tandem binding.
Journal of biomolecular NMR 1998;11(3):307-18.
1998: Dingqiao Yang; Anthony Mittermaier; YK Mok; Lewis E Kay
A study of protein side-chain dynamics from new 2H auto-correlation and 13C cross-correlation NMR experiments: application to the N-terminal SH3 domain from drk.
Journal of molecular biology 1998;276(5):939-54.
1998: Dingqiao Yang; KH Gardner; Lewis E Kay
A Sensitive Pulse Scheme for Measuring the Backbone Dihedral Angle psi Based on Cross-correlation Between (13)C (alpha)- (1)Halpha Dipolar and Carbonyl Chemical Shift Anisotropy Relaxation Interactions.
Journal of biomolecular NMR 1998;11(2):213-20.
1998: Catherine Zwahlen; Sébastien J F Vincent; Lewis E Kay
Analytical description of the effect of adiabatic pulses on IS, I2S, and I3S spin systems.
Journal of magnetic resonance (San Diego, Calif. : 1997) 1998;130(2):169-75.
1998: Lewis E Kay; D Ranjith Muhandiram; G Wolf; S E Shoelson; Julie D Forman-Kay
Correlation between binding and dynamics at SH2 domain interfaces.
Nature structural biology 1998;5(2):156-63.
1998: Jack Greenblatt; Thien-Fah Mah; Pascale Legault; Jeremy Mogridge; Joyce Li; Lewis E Kay
Structure and mechanism in transcriptional antitermination by the bacteriophage lambda N protein.
Cold Spring Harbor symposia on quantitative biology 1998;63():327-36.
1998: Voula Kanelis; NA Farrow; Lewis E Kay; Daniela Rotin; Julie D Forman-Kay
NMR studies of tandem WW domains of Nedd4 in complex with a PY motif-containing region of the epithelial sodium channel.
Biochemistry and cell biology = Biochimie et biologie cellulaire 1998;76(2-3):341-50.
1998: Lewis E Kay
Protein dynamics from NMR.
Biochemistry and cell biology = Biochimie et biologie cellulaire 1998;76(2-3):145-52.
1998: Jeremy Mogridge; Pascale Legault; Joyce Li; Mark Van Oene; Lewis E Kay; Jack Greenblatt
Independent ligand-induced folding of the RNA-binding domain and two functionally distinct antitermination regions in the phage lambda N protein.
Molecular cell 1998;1(2):265-75.
1998: KH Gardner; Lewis E Kay
The use of 2H, 13C, 15N multidimensional NMR to study the structure and dynamics of proteins.
Annual review of biophysics and biomolecular structure 1998;27():357-406.
1997: Dingqiao Yang; YK Mok; Julie D Forman-Kay; NA Farrow; Lewis E Kay
Contributions to protein entropy and heat capacity from bond vector motions measured by NMR spin relaxation.
Journal of molecular biology 1997;272(5):790-804.
1997: D Ranjith Muhandiram; P E Johnson; Dingqiao Yang; O Zhang; L P McIntosh; Lewis E Kay
Specific (15)N, NH correlations for residues in(15) N, (13)C and fractionally deuterated proteins that immediately follow methyl-containing amino acids.
Journal of biomolecular NMR 1997;10(3):283-8.
1997: Lewis E Kay; KH Gardner
Solution NMR spectroscopy beyond 25 kDa.
Current opinion in structural biology 1997;7(5):722-31.
1997: O Zhang; Lewis E Kay; David Shortle; Julie D Forman-Kay
Comprehensive NOE characterization of a partially folded large fragment of staphylococcal nuclease Delta131Delta, using NMR methods with improved resolution.
Journal of molecular biology 1997;272(1):9-20.
1997: SC Li; Zhou Songyang; Sébastien J F Vincent; Catherine Zwahlen; S Wiley; L Cantley; Lewis E Kay; Julie D Forman-Kay; Tony Pawson
High-affinity binding of the Drosophila Numb phosphotyrosine-binding domain to peptides containing a Gly-Pro-(p)Tyr motif.
Proceedings of the National Academy of Sciences of the United States of America 1997;94(14):7204-9.
1997: R Konrat; D Ranjith Muhandiram; NA Farrow; Lewis E Kay
Pulse schemes for the measurement of 3JC'C gamma and 3JNC gamma scalar couplings in 15N,13C uniformly labeled proteins.
Journal of biomolecular NMR 1997;9(4):409-22.
1997: Lawrence P McIntosh; E Brun; Lewis E Kay
Stereospecific assignment of the NH2 resonances from the primary amides of asparagine and glutamine side chains in isotopically labeled proteins.
Journal of biomolecular NMR 1997;9(3):306-12.
1997: NA Farrow; O Zhang; Julie D Forman-Kay; Lewis E Kay
Characterization of the backbone dynamics of folded and denatured states of an SH3 domain.
Biochemistry 1997;36(9):2390-402.
1997: KH Gardner; M K Rosen; Lewis E Kay
Global folds of highly deuterated, methyl-protonated proteins by multidimensional NMR.
Biochemistry 1997;36(6):1389-401.
1997: O Zhang; Julie D Forman-Kay; David Shortle; Lewis E Kay
Triple-resonance NOESY-based experiments with improved spectral resolution: applications to structural characterization of unfolded, partially folded and folded proteins.
Journal of biomolecular NMR 1997;9(2):181-200.
1997: Lewis E Kay
NMR methods for the study of protein structure and dynamics.
Biochemistry and cell biology = Biochimie et biologie cellulaire 1997;75(1):1-15.
1996: N S Rao; Pascale Legault; D Ranjith Muhandiram; Jack Greenblatt; J L Battiste; J R Williamson; Lewis E Kay
NMR pulse schemes for the sequential assignment of arginine side-chain H epsilon protons.
Journal of magnetic resonance. Series B 1996;113(3):272-6.
1996: M K Rosen; KH Gardner; R C Willis; W E Parris; Tony Pawson; Lewis E Kay
Selective methyl group protonation of perdeuterated proteins.
Journal of molecular biology 1996;263(5):627-36.
1996: Dingqiao Yang; Lewis E Kay
Contributions to conformational entropy arising from bond vector fluctuations measured from NMR-derived order parameters: application to protein folding.
Journal of molecular biology 1996;263(2):369-82.
1996: KH Gardner; R Konrat; M K Rosen; Lewis E Kay
An (H)C(CO)NH-TOCSY pulse scheme for sequential assignment of protonated methyl groups in otherwise deuterated (15)N, (13)C-labeled proteins.
Journal of biomolecular NMR 1996;8(3):351-6.
1996: J L Battiste; H Mao; N S Rao; R Tan; D Ranjith Muhandiram; Lewis E Kay; Alan D Frankel; James R Williamson
Alpha helix-RNA major groove recognition in an HIV-1 rev peptide-RRE RNA complex.
Science (New York, N.Y.) 1996;273(5281):1547-51.
1996: M Anafi; M K Rosen; Gerald Gish; Lewis E Kay; Tony Pawson
A potential SH3 domain-binding site in the Crk SH2 domain.
The Journal of biological chemistry 1996;271(35):21365-74.
1996: Megan M McEvoy; D Ranjith Muhandiram; Lewis E Kay; Frederick W Dahlquist
Structure and dynamics of a CheY-binding domain of the chemotaxis kinase CheA determined by nuclear magnetic resonance spectroscopy.
Biochemistry 1996;35(18):5633-40.
1996: K A Williams; NA Farrow; Charles M Deber; Lewis E Kay
Structure and dynamics of bacteriophage IKe major coat protein in MPG micelles by solution NMR.
Biochemistry 1996;35(16):5145-57.
1996: Lewis E Kay; D Ranjith Muhandiram; NA Farrow; Y Aubin; Julie D Forman-Kay
Correlation between dynamics and high affinity binding in an SH2 domain interaction.
Biochemistry 1996;35(2):361-8.
1995: S M Pascal; A U Singer; T Yamazaki; Lewis E Kay; Julie D Forman-Kay
Structural and dynamic characterization of an SH2 domain-phosphopeptide complex by NMR approaches.
Biochemical Society transactions 1995;23(4):729-33.
1995: Megan M McEvoy; H Zhou; A F Roth; D F Lowry; T B Morrison; Lewis E Kay; Frederick W Dahlquist
Nuclear magnetic resonance assignments and global fold of a CheY-binding domain in CheA, the chemotaxis-specific kinase of Escherichia coli.
Biochemistry 1995;34(42):13871-80.
1995: Lewis E Kay
Field gradient techniques in NMR spectroscopy.
Current opinion in structural biology 1995;5(5):674-81.
1995: S M Pascal; T Yamazaki; A U Singer; Lewis E Kay; Julie D Forman-Kay
Structural and dynamic characterization of the phosphotyrosine binding region of a Src homology 2 domain--phosphopeptide complex by NMR relaxation, proton exchange, and chemical shift approaches.
Biochemistry 1995;34(36):11353-62.
1995: NA Farrow; O Zhang; A Szabo; D A Torchia; Lewis E Kay
Spectral density function mapping using 15N relaxation data exclusively.
Journal of biomolecular NMR 1995;6(2):153-62.
1995: G Y Xu; E Ong; Neil Gilkes; Douglas G Kilburn; D Ranjith Muhandiram; Marees Harris-Brandts; J P Carver; Lewis E Kay; T S Harvey
Solution structure of a cellulose-binding domain from Cellulomonas fimi by nuclear magnetic resonance spectroscopy.
Biochemistry 1995;34(21):6993-7009.
1995: M K Rosen; T Yamazaki; Gerald Gish; C M Kay; Tony Pawson; Lewis E Kay
Direct demonstration of an intramolecular SH2-phosphotyrosine interaction in the Crk protein.
Nature 1995;374(6521):477-9.
1995: NA Farrow; O Zhang; Julie D Forman-Kay; Lewis E Kay
Comparison of the backbone dynamics of a folded and an unfolded SH3 domain existing in equilibrium in aqueous buffer.
Biochemistry 1995;34(3):868-78.
1994: O Zhang; Lewis E Kay; Jean Paul olivier; Julie D Forman-Kay
Backbone 1H and 15N resonance assignments of the N-terminal SH3 domain of drk in folded and unfolded states using enhanced-sensitivity pulsed field gradient NMR techniques.
Journal of biomolecular NMR 1994;4(6):845-58.
1994: NA Farrow; O Zhang; Julie D Forman-Kay; Lewis E Kay
A heteronuclear correlation experiment for simultaneous determination of 15N longitudinal decay and chemical exchange rates of systems in slow equilibrium.
Journal of biomolecular NMR 1994;4(5):727-34.
1994: W Lee; M J Revington; Cheryl H Arrowsmith; Lewis E Kay
A pulsed field gradient isotope-filtered 3D 13C HMQC-NOESY experiment for extracting intermolecular NOE contacts in molecular complexes.
FEBS letters 1994;350(1):87-90.
1994: NA Farrow; R Muhandiram; A U Singer; S M Pascal; C M Kay; Gerald Gish; S E Shoelson; Tony Pawson; Julie D Forman-Kay; Lewis E Kay
Backbone dynamics of a free and phosphopeptide-complexed Src homology 2 domain studied by 15N NMR relaxation.
Biochemistry 1994;33(19):5984-6003.
1994: S M Pascal; A U Singer; Gerald Gish; T Yamazaki; S E Shoelson; Tony Pawson; Lewis E Kay; Julie D Forman-Kay
Nuclear magnetic resonance structure of an SH2 domain of phospholipase C-gamma 1 complexed with a high affinity binding peptide.
Cell 1994;77(3):461-72.
1994: Stefan Bagby; T S Harvey; Lewis E Kay; S G Eagle; S Inouye; Mitsuhiko Ikura
Unusual helix-containing greek keys in development-specific Ca(2+)-binding protein S. 1H, 15N, and 13C assignments and secondary structure determined with the use of multidimensional double and triple resonance heteronuclear NMR spectroscopy.
Biochemistry 1994;33(9):2409-21.
1992: Gaetano Barbato; M Ikura; Lewis E Kay; R W Pastor; Adriaan Bax
Backbone dynamics of calmodulin studied by 15N relaxation using inverse detected two-dimensional NMR spectroscopy: the central helix is flexible.
Biochemistry 1992;31(23):5269-78.
1991: M Ikura; S Spera; Gaetano Barbato; Lewis E Kay; M Krinks; Adriaan Bax
Secondary structure and side-chain 1H and 13C resonance assignments of calmodulin in solution by heteronuclear multidimensional NMR spectroscopy.
Biochemistry 1991;30(38):9216-28.
1991: M Ikura; Lewis E Kay; Adriaan Bax
Improved three-dimensional 1H-13C-1H correlation spectroscopy of a 13C-labeled protein using constant-time evolution.
Journal of biomolecular NMR 1991;1(3):299-304.
1991: M Ikura; Lewis E Kay; M Krinks; Adriaan Bax
Triple-resonance multidimensional NMR study of calmodulin complexed with the binding domain of skeletal muscle myosin light-chain kinase: indication of a conformational change in the central helix.
Biochemistry 1991;30(22):5498-504.
1991: Lewis E Kay; Julie D Forman-Kay; W D McCubbin; C M Kay
Solution structure of a polypeptide dimer comprising the fourth Ca(2+)-binding site of troponin C by nuclear magnetic resonance spectroscopy.
Biochemistry 1991;30(17):4323-33.
1991: G Marius Clore; Lewis E Kay; Adriaan Bax; Angela M Gronenborn
Four-dimensional 13C/13C-edited nuclear Overhauser enhancement spectroscopy of a protein in solution: application to interleukin 1 beta.
Biochemistry 1991;30(1):12-8.
1991: Adriaan Bax; M Ikura; Lewis E Kay; Gaetano Barbato; S Spera
Multidimensional triple resonance NMR spectroscopy of isotopically uniformly enriched proteins: a powerful new strategy for structure determination.
Ciba Foundation symposium 1991;161():108-19; discussion 119-35.
1990: Lewis E Kay; G Marius Clore; Adriaan Bax; Angela M Gronenborn
Four-dimensional heteronuclear triple-resonance NMR spectroscopy of interleukin-1 beta in solution.
Science (New York, N.Y.) 1990;249(4967):411-4.
1990: M Ikura; D Marion; Lewis E Kay; H Shih; M Krinks; C B Klee; Adriaan Bax
Heteronuclear 3D NMR and isotopic labeling of calmodulin. Towards the complete assignment of the 1H NMR spectrum.
Biochemical pharmacology 1990;40(1):153-60.
1990: M Ikura; Lewis E Kay; Adriaan Bax
A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.
Biochemistry 1990;29(19):4659-67.
1990: J E Forman-Kay; Angela M Gronenborn; Lewis E Kay; Paul T Wingfield; G Marius Clore
Studies on the solution conformation of human thioredoxin using heteronuclear 15N-1H nuclear magnetic resonance spectroscopy.
Biochemistry 1990;29(6):1566-72.
1989: Lewis E Kay; D A Torchia; Adriaan Bax
Backbone dynamics of proteins as studied by 15N inverse detected heteronuclear NMR spectroscopy: application to staphylococcal nuclease.
Biochemistry 1989;28(23):8972-9.
1989: D Marion; P C Driscoll; Lewis E Kay; Paul T Wingfield; Adriaan Bax; Angela M Gronenborn; G Marius Clore
Overcoming the overlap problem in the assignment of 1H NMR spectra of larger proteins by use of three-dimensional heteronuclear 1H-15N Hartmann-Hahn-multiple quantum coherence and nuclear Overhauser-multiple quantum coherence spectroscopy: application to interleukin 1 beta.
Biochemistry 1989;28(15):6150-6.