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Chaitan Khosla

Research Profile

Chemicals & Drugs

Concepts & Ideas

Living Beings

Genes & Molecular Sequences

Amino Acid Sequences

Disorders

Celiac Disease

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Publications

TOP 3
Huanbin Xu; Ronald S Veazey; Xiaolei Wang; Pyone Aye; Juan T Borda; Gaby G Doxiadis; Jason Dufour; Stephanie L Feely; Chaitan Khosla; Weiwei Li; David X Liu; Karol Sestak
Gluten-sensitive enteropathy coincides with decreased capability of intestinal T cells to secrete IL-17 and IL-22 in a macaque model for celiac disease.
Briana J Dunn; David E Cane; Chaitan Khosla
Mechanism and specificity of an acyltransferase domain from a modular polyketide synthase.
Jay T Fitzgerald; Louise K Charkoudian; Katharine R Watts; Chaitan Khosla
Analysis and refactoring of the A-74528 biosynthetic pathway.

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All Publications

2013: Huanbin Xu; Ronald S Veazey; Xiaolei Wang; Pyone Aye; Juan T Borda; Gaby G Doxiadis; Jason Dufour; Stephanie L Feely; Chaitan Khosla; Weiwei Li; David X Liu; Karol Sestak
Gluten-sensitive enteropathy coincides with decreased capability of intestinal T cells to secrete IL-17 and IL-22 in a macaque model for celiac disease.
Clinical immunology (Orlando, Fla.) 2013;147(1):40-9.
2013: Briana J Dunn; David E Cane; Chaitan Khosla
Mechanism and specificity of an acyltransferase domain from a modular polyketide synthase.
Biochemistry 2013;52(11):1839-41.
2013: Jay T Fitzgerald; Louise K Charkoudian; Katharine R Watts; Chaitan Khosla
Analysis and refactoring of the A-74528 biosynthetic pathway.
Journal of the American Chemical Society 2013;135(10):3752-5.
2013: Thomas R DiRaimondo; Xi Jin; Nicholas M Plugis; Chaitan Khosla
Selective inhibition of extracellular thioredoxin by asymmetric disulfides.
Journal of medicinal chemistry 2013;56(3):1301-10.
2012: Cornelius Klöck; Chaitan Khosla
Regulation of the activities of the mammalian transglutaminase family of enzymes.
Protein science : a publication of the Protein Society 2012;21(12):1781-91.
2012: Tracy C Holmes; Masato Iwatsuki; Joseph L DeRisi; Michael A Fischbach; Aaron E May; Satoshi Omura; J Graham Ruby; Peter Skewes-Cox; Kathia Zaleta-Rivera; Chaitan Khosla
Molecular insights into the biosynthesis of guadinomine: a type III secretion system inhibitor.
Journal of the American Chemical Society 2012;134(42):17797-806.
2012: Colin J B Harvey; Vijay S Pande; Joseph D Puglisi; David E Cane; Chaitan Khosla
Precursor directed biosynthesis of an orthogonally functional erythromycin analogue: selectivity in the ribosome macrolide binding pocket.
Journal of the American Chemical Society 2012;134(29):12259-65.
2012: Cornelius Klöck; Thomas R Diraimondo; Chaitan Khosla
Role of transglutaminase 2 in celiac disease pathogenesis.
Seminars in immunopathology 2012;34(4):513-22.
2012: Satoshi Yuzawa; David E Cane; Shiven Kapur; Chaitan Khosla
Role of a conserved arginine residue in linkers between the ketosynthase and acyltransferase domains of multimodular polyketide synthases.
Biochemistry 2012;51(18):3708-10.
2012: Jianhao Wang; Xi Jin; Chaitan Khosla; Jiahui Liu; Jiang Xia
Resolving multiple protein-peptide binding events: implication for HLA-DQ2 mediated antigen presentation in celiac disease.
Chemistry, an Asian journal 2012;7(5):992-9.
2012: Fong T Wong; Chaitan Khosla
Combinatorial biosynthesis of polyketides--a perspective.
Current opinion in chemical biology 2012;16(1-2):117-23.
2012: Thomas R Diraimondo; Cornelius Klöck; Chaitan Khosla
Interferon-γ activates transglutaminase 2 via a phosphatidylinositol-3-kinase-dependent pathway: implications for celiac sprue therapy.
The Journal of pharmacology and experimental therapeutics 2012;341(1):104-14.
2012: Shiven Kapur; Sanketha Kenthirapalan; David E Cane; Alice Y Chen; Brian Lowry; Satoshi Yuzawa; Chaitan Khosla
Reprogramming a module of the 6-deoxyerythronolide B synthase for iterative chain elongation.
Proceedings of the National Academy of Sciences of the United States of America 2012;109(11):4110-5.
2012: Laila Dafik; Ludvig M Sollid; Jorunn Stamnaes; Megan Albertelli; Chaitan Khosla
Activation and inhibition of transglutaminase 2 in mice.
PloS one 2012;7(2):e30642.
2011: Xingye Yu; Fayin Zhu; Tiangang Liu; Chaitan Khosla
In vitro reconstitution and steady-state analysis of the fatty acid synthase from Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America 2011;108(46):18643-8.
2011: Xi Jin; Cornelius Klöck; Ludvig M Sollid; Jorunn Stamnaes; Thomas R DiRaimondo; Chaitan Khosla
Activation of extracellular transglutaminase 2 by thioredoxin.
The Journal of biological chemistry 2011;286(43):37866-73.
2011: Pouya Javidpour; Chaitan Khosla; Abhirup Das; Shiou-Chuan Tsai
Structural and biochemical studies of the hedamycin type II polyketide ketoreductase (HedKR): molecular basis of stereo- and regiospecificities.
Biochemistry 2011;50(34):7426-39.
2011: Ping-Hui Szu; Michael J Meehan; Jeremy Minshull; Don D Nguyen; Abhirup Das; Pieter dorrestein; Sridhar Govindarajan; Chaitan Khosla
Analysis of the ketosynthase-chain length factor heterodimer from the fredericamycin polyketide synthase.
Chemistry & biology 2011;18(8):1021-31.
2011: Fong T Wong; Irimpan I Mathews; David E Cane; Xi Jin; Chaitan Khosla
Structure and mechanism of the trans-acting acyltransferase from the disorazole synthase.
Biochemistry 2011;50(30):6539-48.
2011: Louise K Charkoudian; Shiven Kapur; David E Cane; Stefania Capone; Corey W Liu; Dieter Seebach; Antonio Togni; Chaitan Khosla
Probing the interactions of an acyl carrier protein domain from the 6-deoxyerythronolide B synthase.
Protein science : a publication of the Protein Society 2011;20(7):1244-55.
2011: L M Sollid; Chaitan Khosla
Novel therapies for coeliac disease.
Journal of internal medicine 2011;269(6):604-13.
2011: Liya Yuan; Tracy C Holmes; R Edward Watts; Chaitan Khosla; Thomas J Broekelmann; Robert Mecham; Hong Zheng; Enrique W Izaguirre; Keith M Rich
Novel chemo-sensitizing agent, ERW1227B, impairs cellular motility and enhances cell death in glioblastomas.
Journal of neuro-oncology 2011;103(2):207-19.
2011: Cornelius Klöck; Xi Jin; Kihang Choi; Chaitan Khosla; Peter B Madrid; Andrew Spencer; Brian C Raimundo; Paul Boardman; Guido Lanza; John H Griffin
Acylideneoxoindoles: a new class of reversible inhibitors of human transglutaminase 2.
Bioorganic & medicinal chemistry letters 2011;21(9):2692-6.
2011: Younjoo Lee; Jun Yong Choi; Hong Fu; Colin Harvey; Sandeep Ravindran; William R Roush; John C Boothroyd; Chaitan Khosla
Chemistry and biology of macrolide antiparasitic agents.
Journal of medicinal chemistry 2011;54(8):2792-804.
2011: Laila Dafik; Chaitan Khosla
Dihydroisoxazole analogs for labeling and visualization of catalytically active transglutaminase 2.
Chemistry & biology 2011;18(1):58-66.
2011: Ho Young Lee; Colin J B Harvey; David E Cane; Chaitan Khosla
Improved precursor-directed biosynthesis in E. coli via directed evolution.
The Journal of antibiotics 2011;64(1):59-64.
2010: Shiven Kapur; Alice Y Chen; David E Cane; Chaitan Khosla
Molecular recognition between ketosynthase and acyl carrier protein domains of the 6-deoxyerythronolide B synthase.
Proceedings of the National Academy of Sciences of the United States of America 2010;107(51):22066-71.
2010: Chiara R Valenzano; Young-Ok You; Ashish Garg; Adrian Keatinge-Clay; Chaitan Khosla; David E Cane
Stereospecificity of the dehydratase domain of the erythromycin polyketide synthase.
Journal of the American Chemical Society 2010;132(42):14697-9.
2010: Tiangang Liu; Chaitan Khosla
Chemistry. A balancing act for Taxol precursor pathways in E. coli.
Science (New York, N.Y.) 2010;330(6000):44-5.
2010: Chaitan Khosla
Thematic minireview series on antibacterial natural products: new tricks for old dogs.
The Journal of biological chemistry 2010;285(36):27499.
2010: Jorunn Stamnaes; Daniel M Pinkas; Burkhard Fleckenstein; Chaitan Khosla; Ludvig M Sollid
Redox regulation of transglutaminase 2 activity.
The Journal of biological chemistry 2010;285(33):25402-9.
2010: Kathia Zaleta-Rivera; Louise K Charkoudian; Christian P Ridley; Chaitan Khosla
Cloning, sequencing, heterologous expression, and mechanistic analysis of A-74528 biosynthesis.
Journal of the American Chemical Society 2010;132(26):9122-8.
2010: Abhirup Das; Ping-Hui Szu; Jay T Fitzgerald; Chaitan Khosla
Mechanism and engineering of polyketide chain initiation in fredericamycin biosynthesis.
Journal of the American Chemical Society 2010;132(26):8831-3.
2010: Ivana Matic; Alessandra Sacchi; Alessandra Rinaldi; Gennaro Melino; Chaitan Khosla; Laura Falasca; Mauro Piacentini
Characterization of transglutaminase type II role in dendritic cell differentiation and function.
Journal of leukocyte biology 2010;88(1):181-8.
2010: Tiangang Liu; Harmit Vora; Chaitan Khosla
Quantitative analysis and engineering of fatty acid biosynthesis in E. coli.
Metabolic engineering 2010;12(4):378-86.
2010: Jennifer A Sealey-Voyksner; Chaitan Khosla; Robert D Voyksner; James W Jorgenson
Novel aspects of quantitation of immunogenic wheat gluten peptides by liquid chromatography-mass spectrometry/mass spectrometry.
Journal of chromatography. A 2010;1217(25):4167-83.
2010: Elizabeth Tahanian; Simon Lord-Dufour; Abhirup Das; Chaitan Khosla; René Roy; Borhane Annabi
Inhibition of tubulogenesis and of carcinogen-mediated signaling in brain endothelial cells highlight the antiangiogenic properties of a mumbaistatin analog.
Chemical biology & drug design 2010;75(5):481-8.
2010: Fong T Wong; Alice Y Chen; David E Cane; Chaitan Khosla
Protein-protein recognition between acyltransferases and acyl carrier proteins in multimodular polyketide synthases.
Biochemistry 2010;49(1):95-102.
2010: Louise K Charkoudian; Jay T Fitzgerald; Chaitan Khosla; Andrea Champlin
In living color: bacterial pigments as an untapped resource in the classroom and beyond.
PLoS biology 2010;8(10):.
2010: Tiangang Liu; Chaitan Khosla
Genetic engineering of Escherichia coli for biofuel production.
Annual review of genetics 2010;44():53-69.
2010: Kaushiki Mazumdar; Xavier Alvarez; Juan T Borda; Jason Dufour; Edith Martin; Michael T Bethune; Chaitan Khosla; Karol Sestak
Visualization of transepithelial passage of the immunogenic 33-residue peptide from alpha-2 gliadin in gluten-sensitive macaques.
PloS one 2010;5(4):e10228.
2009: Chiara R Valenzano; Rachel J Lawson; Alice Y Chen; Chaitan Khosla; David E Cane
The biochemical basis for stereochemical control in polyketide biosynthesis.
Journal of the American Chemical Society 2009;131(51):18501-11.
2009: Abhirup Das; Chaitan Khosla
In vivo and in vitro analysis of the hedamycin polyketide synthase.
Chemistry & biology 2009;16(11):1197-207.
2009: Chaitan Khosla
Structures and mechanisms of polyketide synthases.
The Journal of organic chemistry 2009;74(17):6416-20.
2009: Michael T Bethune; Mónica Crespo-Bosque; Elin Bergseng; Kaushiki Mazumdar; Lara Doyle; Karol Sestak; Ludvig M Sollid; Chaitan Khosla
Noninflammatory gluten peptide analogs as biomarkers for celiac sprue.
Chemistry & biology 2009;16(8):868-81.
2009: Abhirup Das; Chaitan Khosla
Biosynthesis of aromatic polyketides in bacteria.
Accounts of chemical research 2009;42(5):631-9.
2009: Michael T Bethune; Matthew Siegel; Samuel Howles-Banerji; Chaitan Khosla
Interferon-gamma released by gluten-stimulated celiac disease-specific intestinal T cells enhances the transepithelial flux of gluten peptides.
The Journal of pharmacology and experimental therapeutics 2009;329(2):657-68.
2009: Chaitan Khosla; Shiven Kapur; David E Cane
Revisiting the modularity of modular polyketide synthases.
Current opinion in chemical biology 2009;13(2):135-43.
2009: Simon Lord-Dufour; Ian B Copland; Louis-Charles Levros; Martin Post; Abhirup Das; Chaitan Khosla; Jacques Galipeau; Eric Rassart; Borhane Annabi
Evidence for transcriptional regulation of the glucose-6-phosphate transporter by HIF-1alpha: Targeting G6PT with mumbaistatin analogs in hypoxic mesenchymal stromal cells.
Stem cells (Dayton, Ohio) 2009;27(3):489-97.
2009: Jennifer Ehren; Belen Morón; Edith Martin; Michael T Bethune; Gary M Gray; Chaitan Khosla
A food-grade enzyme preparation with modest gluten detoxification properties.
PloS one 2009;4(7):e6313.
2008: Jennifer Ehren; Sridhar Govindarajan; Belén Morón; Jeremy Minshull; Chaitan Khosla
Protein engineering of improved prolyl endopeptidases for celiac sprue therapy.
Protein engineering, design & selection : PEDS 2008;21(12):699-707.
2008: Xuefeng Lu; Harmit Vora; Chaitan Khosla
Overproduction of free fatty acids in E. coli: implications for biodiesel production.
Metabolic engineering 2008;10(6):333-9.
2008: Liya Yuan; Amir Behdad; Matthew Siegel; Chaitan Khosla; Ryuji Higashikubo; Keith M Rich
Tissue transgluaminase 2 expression in meningiomas.
Journal of neuro-oncology 2008;90(2):125-32.
2008: Roselyne Castonguay; Chiara R Valenzano; Alice Y Chen; Adrian Keatinge-Clay; Chaitan Khosla; David E Cane
Stereospecificity of ketoreductase domains 1 and 2 of the tylactone modular polyketide synthase.
Journal of the American Chemical Society 2008;130(35):11598-9.
2008: Shiven Kapur; Chaitan Khosla
Biochemistry: Fit for an enzyme.
Nature 2008;454(7206):832-3.
2008: Shiven Kapur; Andrew Worthington; Yinyan Tang; David E Cane; Michael D Burkart; Chaitan Khosla
Mechanism based protein crosslinking of domains from the 6-deoxyerythronolide B synthase.
Bioorganic & medicinal chemistry letters 2008;18(10):3034-8.
2008: Christian P Ridley; Ho Young Lee; Chaitan Khosla
Evolution of polyketide synthases in bacteria.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(12):4595-600.
2008: Michael T Bethune; Chaitan Khosla
Parallels between pathogens and gluten peptides in celiac sprue.
PLoS pathogens 2008;4(2):e34.
2008: Belén Morón; Michael T Bethune; Isabel Comino; Hamid Manyani; Marina Ferragud; Manuel Carlos López; Angel Cebolla; Chaitan Khosla; Carolina Sousa
Toward the assessment of food toxicity for celiac patients: characterization of monoclonal antibodies to a main immunogenic gluten peptide.
PloS one 2008;3(5):e2294.
2008: Matthew Siegel; Pavel Strnad; R Edward Watts; Kihang Choi; Bana Jabri; Bishr Omary; Chaitan Khosla
Extracellular transglutaminase 2 is catalytically inactive, but is transiently activated upon tissue injury.
PloS one 2008;3(3):e1861.
2008: Michael T Bethune; Erin Ribka; Chaitan Khosla; Karol Sestak
Transepithelial transport and enzymatic detoxification of gluten in gluten-sensitive rhesus macaques.
PloS one 2008;3(3):e1857.
2008: Michael T Bethune; Juan T Borda; Erin Ribka; Michael-Xun Liu; Kathrine Phillippi-Falkenstein; Ronald J Jandacek; Gaby G M Doxiadis; Gary M Gray; Chaitan Khosla; Karol Sestak
A non-human primate model for gluten sensitivity.
PloS one 2008;3(2):e1614.
2007: Daniel M Pinkas; Pavel Strop; Axel T Brunger; Chaitan Khosla
Transglutaminase 2 undergoes a large conformational change upon activation.
PLoS biology 2007;5(12):e327.
2007: Roselyne Castonguay; Weiguo He; Alice Y Chen; Chaitan Khosla; David E Cane
Stereospecificity of ketoreductase domains of the 6-deoxyerythronolide B synthase.
Journal of the American Chemical Society 2007;129(44):13758-69.
2007: Jiang Xia; Elin Bergseng; Burkhard Fleckenstein; Matthew Siegel; Chu-Young Kim; Chaitan Khosla; Ludvig M Sollid
Cyclic and dimeric gluten peptide analogues inhibiting DQ2-mediated antigen presentation in celiac disease.
Bioorganic & medicinal chemistry 2007;15(20):6565-73.
2007: Viktor Y Alekseyev; Corey W Liu; David E Cane; Joseph D Puglisi; Chaitan Khosla
Solution structure and proposed domain domain recognition interface of an acyl carrier protein domain from a modular polyketide synthase.
Protein science : a publication of the Protein Society 2007;16(10):2093-107.
2007: Matthew Siegel; Jiang Xia; Chaitan Khosla
Structure-based design of alpha-amido aldehyde containing gluten peptide analogues as modulators of HLA-DQ2 and transglutaminase 2.
Bioorganic & medicinal chemistry 2007;15(18):6253-61.
2007: Harmit Vora; James McIntire; Pawan Kumar; Milind Deshpande; Chaitan Khosla
A scaleable manufacturing process for pro-EP-B2, a cysteine protease from barley indicated for celiac sprue.
Biotechnology and bioengineering 2007;98(1):177-85.
2007: Yinyan Tang; Alice Y Chen; Chu-Young Kim; David E Cane; Chaitan Khosla
Structural and mechanistic analysis of protein interactions in module 3 of the 6-deoxyerythronolide B synthase.
Chemistry & biology 2007;14(8):931-43.
2007: Jonathan Gass; Michael T Bethune; Matthew Siegel; Andrew Spencer; Chaitan Khosla
Combination enzyme therapy for gastric digestion of dietary gluten in patients with celiac sprue.
Gastroenterology 2007;133(2):472-80.
2007: Matthew Siegel; Chaitan Khosla
Transglutaminase 2 inhibitors and their therapeutic role in disease states.
Pharmacology & therapeutics 2007;115(2):232-45.
2007: Taek Soon Lee; Abhirup Das; Chaitan Khosla
Structure-activity relationships of semisynthetic mumbaistatin analogs.
Bioorganic & medicinal chemistry 2007;15(15):5207-18.
2007: Alice Y Chen; David E Cane; Chaitan Khosla
Structure-based dissociation of a type I polyketide synthase module.
Chemistry & biology 2007;14(7):784-92.
2007: Jonathan Gass; Harmit Vora; Alan Hofmann; Gary M Gray; Chaitan Khosla
Enhancement of dietary protein digestion by conjugated bile acids.
Gastroenterology 2007;133(1):16-23.
2007: L Yuan; M Siegel; Kihang Choi; Chaitan Khosla; Ryan Miller; E N Jackson; D Piwnica-Worms; Keith M Rich
Transglutaminase 2 inhibitor, KCC009, disrupts fibronectin assembly in the extracellular matrix and sensitizes orthotopic glioblastomas to chemotherapy.
Oncogene 2007;26(18):2563-73.
2007: Leonard Katz; Chaitan Khosla
Antibiotic production from the ground up.
Nature biotechnology 2007;25(4):428-9.
2007: Pavel Strnad; Masaru Harada; Matthew Siegel; Robert Terkeltaub; Robert M Graham; Chaitan Khosla; Bishr Omary
Transglutaminase 2 regulates mallory body inclusion formation and injury-associated liver enlargement.
Gastroenterology 2007;132(4):1515-26.
2007: Martha Lovato Tse; R Edward Watts; Chaitan Khosla
Substrate tolerance of module 6 of the epothilone synthetase.
Biochemistry 2007;46(11):3385-93.
2007: Christian P Ridley; Chaitan Khosla
Synthesis and biological activity of novel pyranopyrones derived from engineered aromatic polyketides.
ACS chemical biology 2007;2(2):104-8.
2007: Ho Young Lee; Chaitan Khosla
Bioassay-guided evolution of glycosylated macrolide antibiotics in Escherichia coli.
PLoS biology 2007;5(2):e45.
2007: Jonathan Gass; Chaitan Khosla
Prolyl endopeptidases.
Cellular and molecular life sciences : CMLS 2007;64(3):345-55.
2007: Chaitan Khosla; Yinyan Tang; Alice Y Chen; Nathan A Schnarr; David E Cane
Structure and mechanism of the 6-deoxyerythronolide B synthase.
Annual review of biochemistry 2007;76():195-221.
2006: Nathan A Schnarr; Chaitan Khosla
Trapping transient protein-protein interactions in polyketide biosynthesis.
ACS chemical biology 2006;1(11):679-80.
2006: R Edward Watts; Matthew Siegel; Chaitan Khosla
Structure-activity relationship analysis of the selective inhibition of transglutaminase 2 by dihydroisoxazoles.
Journal of medicinal chemistry 2006;49(25):7493-501.
2006: Yinyan Tang; Ho Young Lee; Yi Tang; Chu-Young Kim; Irimpan I Mathews; Chaitan Khosla
Structural and functional studies on SCO1815: a beta-ketoacyl-acyl carrier protein reductase from Streptomyces coelicolor A3(2).
Biochemistry 2006;45(47):14085-93.
2006: Jonathan Gass; Harmit Vora; Michael T Bethune; Gary M Gray; Chaitan Khosla
Effect of barley endoprotease EP-B2 on gluten digestion in the intact rat.
The Journal of pharmacology and experimental therapeutics 2006;318(3):1178-86.
2006: Mathew A Rude; Chaitan Khosla
Production of ansamycin polyketide precursors in Escherichia coli.
The Journal of antibiotics 2006;59(8):464-70.
2006: Yinyan Tang; Chu-Young Kim; Irimpan I Mathews; David E Cane; Chaitan Khosla
The 2.7-Angstrom crystal structure of a 194-kDa homodimeric fragment of the 6-deoxyerythronolide B synthase.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(30):11124-9.
2006: Matthew Siegel; Michael T Bethune; Jonathan Gass; Jennifer Ehren; Jiang Xia; Alexandre Johannsen; Tor Stuge; Gary M Gray; Peter P Lee; Chaitan Khosla
Rational design of combination enzyme therapy for celiac sprue.
Chemistry & biology 2006;13(6):649-58.
2006: Michael T Bethune; Pavel Strop; Yinyan Tang; Ludvig M Sollid; Chaitan Khosla
Heterologous expression, purification, refolding, and structural-functional characterization of EP-B2, a self-activating barley cysteine endoprotease.
Chemistry & biology 2006;13(6):637-47.
2006: Leslie M Hicks; Matthew T Mazur; Leah M. Miller; Pieter dorrestein; Nathan A Schnarr; Chaitan Khosla; Neil L Kelleher
Investigating nonribosomal peptide and polyketide biosynthesis by direct detection of intermediates on >70 kDa polypeptides by using Fourier-transform mass spectrometry.
Chembiochem : a European journal of chemical biology 2006;7(6):904-7.
2006: Pavel Strnad; Matthew Siegel; Diana M Toivola; Kihang Choi; Jon C Kosek; Chaitan Khosla; Bishr Omary
Pharmacologic transglutaminase inhibition attenuates drug-primed liver hypertrophy but not Mallory body formation.
FEBS letters 2006;580(9):2351--2357.
2006: Alice Y Chen; Nathan A Schnarr; Chu-Young Kim; David E Cane; Chaitan Khosla
Extender unit and acyl carrier protein specificity of ketosynthase domains of the 6-deoxyerythronolide B synthase.
Journal of the American Chemical Society 2006;128(9):3067-74.
2006: Jiang Xia; Matthew Siegel; Elin Bergseng; Ludvig M Sollid; Chaitan Khosla
Inhibition of HLA-DQ2-mediated antigen presentation by analogues of a high affinity 33-residue peptide from alpha2-gliadin.
Journal of the American Chemical Society 2006;128(6):1859-67.
2006: Weiguo He; Jiaquan Wu; Chaitan Khosla; David E Cane
Macrolactonization to 10-deoxymethynolide catalyzed by the recombinant thioesterase of the picromycin/methymycin polyketide synthase.
Bioorganic & medicinal chemistry letters 2006;16(2):391-4.
2006: David A Moffet; Chaitan Khosla; David E Cane
Modular polyketide synthases: Investigating intermodular communication using 6 deoxyerythronolide B synthase module 2.
Bioorganic & medicinal chemistry letters 2006;16(1):213-6.
2005: Jonathan Gass; Jennifer Ehren; Gregg Strohmeier; Indu Isaacs; Chaitan Khosla
Fermentation, purification, formulation, and pharmacological evaluation of a prolyl endopeptidase from Myxococcus xanthus: implications for Celiac Sprue therapy.
Biotechnology and bioengineering 2005;92(6):674-84.
2005: Jiaquan Wu; Toby J Zaleski; Chiara Valenzano; Chaitan Khosla; David E Cane
Polyketide double bond biosynthesis. Mechanistic analysis of the dehydratase-containing module 2 of the picromycin/methymycin polyketide synthase.
Journal of the American Chemical Society 2005;127(49):17393-404.
2005: Jiaquan Wu; Weiguo He; Chaitan Khosla; David E Cane
Chain elongation, macrolactonization, and hydrolysis of natural and reduced hexaketide substrates by the picromycin/methymycin polyketide synthase.
Angewandte Chemie (International ed. in English) 2005;44(46):7557-60.
2005: Taek Soon Lee; Chaitan Khosla; Yi Tang
Orthogonal protein interactions in spore pigment producing and antibiotic producing polyketide synthases.
The Journal of antibiotics 2005;58(10):663-6.
2005: Chaitan Khosla; Gary M Gray; Ludvig M Sollid
Putative efficacy and dosage of prolyl endopeptidase for digesting and detoxifying gliadin peptides.
Gastroenterology 2005;129(4):1362-3; author reply 1363.
2005: Taek Soon Lee; Chaitan Khosla; Yi Tang
Engineered biosynthesis of aklanonic acid analogues.
Journal of the American Chemical Society 2005;127(35):12254-62.
2005: Nathan A Schnarr; Alice Y Chen; David E Cane; Chaitan Khosla
Analysis of covalently bound polyketide intermediates on 6-deoxyerythronolide B synthase by tandem proteolysis-mass spectrometry.
Biochemistry 2005;44(35):11836-42.
2005: Lu Shan; Shuo-Wang Qiao; Helene Arentz-Hansen; Øyvind Molberg; Gary M Gray; Ludvig M Sollid; Chaitan Khosla
Identification and analysis of multivalent proteolytically resistant peptides from gluten: implications for celiac sprue.
Journal of proteome research 2005;4(5):1732-41.
2005: Liya Yuan; Kihang Choi; Chaitan Khosla; Xiao Zheng; Ryuji Higashikubo; Michael R Chicoine; Keith M Rich
Tissue transglutaminase 2 inhibition promotes cell death and chemosensitivity in glioblastomas.
Molecular cancer therapeutics 2005;4(9):1293-302.
2005: Nathan A Schnarr; Chaitan Khosla
Biological chemistry: just add chlorine.
Nature 2005;436(7054):1094-5.
2005: Ingo V Hartung; Mathew A Rude; Nathan A Schnarr; Daniel Hunziker; Chaitan Khosla
Stereochemical assignment of intermediates in the rifamycin biosynthetic pathway by precursor-directed biosynthesis.
Journal of the American Chemical Society 2005;127(32):11202-3.
2005: Gail G Pyle; Brian Paaso; Barbara E Anderson; Diane D Allen; Thomas Marti; Qing Li; Matthew Siegel; Chaitan Khosla; Gary M Gray
Effect of pretreatment of food gluten with prolyl endopeptidase on gluten-induced malabsorption in celiac sprue.
Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association 2005;3(7):687-94.
2005: Gail G Pyle; Brian Paaso; Barbara E Anderson; Diane Allen; Thomas Marti; Chaitan Khosla; Gary M Gray
Low-dose gluten challenge in celiac sprue: malabsorptive and antibody responses.
Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association 2005;3(7):679-86.
2005: Elin Bergseng; Jiang Xia; Chu-Young Kim; Chaitan Khosla; Ludvig M Sollid
Main chain hydrogen bond interactions in the binding of proline-rich gluten peptides to the celiac disease-associated HLA-DQ2 molecule.
The Journal of biological chemistry 2005;280(23):21791-6.
2005: Chaitan Khosla; Yi Tang
Chemistry. A new route to designer antibiotics.
Science (New York, N.Y.) 2005;308(5720):367-8.
2005: Kihang Choi; Matthew Siegel; Justin L Piper; Liya Yuan; Eun Cho; Pavel Strnad; Bishr Omary; Keith M Rich; Chaitan Khosla
Chemistry and biology of dihydroisoxazole derivatives: selective inhibitors of human transglutaminase 2.
Chemistry & biology 2005;12(4):469-75.
2005: Jiang Xia; Ludvig M Sollid; Chaitan Khosla
Equilibrium and kinetic analysis of the unusual binding behavior of a highly immunogenic gluten peptide to HLA-DQ2.
Biochemistry 2005;44(11):4442-9.
2005: Lu Shan; Irimpan I Mathews; Chaitan Khosla
Structural and mechanistic analysis of two prolyl endopeptidases: role of interdomain dynamics in catalysis and specificity.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(10):3599-604.
2005: Ludvig M Sollid; Chaitan Khosla
Future therapeutic options for celiac disease.
Nature clinical practice. Gastroenterology & hepatology 2005;2(3):140-7.
2005: Shuo-Wang Qiao; Justin Piper; Guttorm Haraldsen; Inger Oynebråten; Burkhard Fleckenstein; Oyvind Molberg; Chaitan Khosla; Ludvig M Sollid
Tissue transglutaminase-mediated formation and cleavage of histamine-gliadin complexes: biological effects and implications for celiac disease.
Journal of immunology (Baltimore, Md. : 1950) 2005;174(3):1657-63.
2005: Thomas Marti; Oyvind Molberg; Qing Li; Gary M Gray; Chaitan Khosla; Ludvig M Sollid
Prolyl endopeptidase-mediated destruction of T cell epitopes in whole gluten: chemical and immunological characterization.
The Journal of pharmacology and experimental therapeutics 2005;312(1):19-26.
2004: Jiaquan Wu; Kenji Kinoshita; Chaitan Khosla; David E Cane
Biochemical analysis of the substrate specificity of the beta-ketoacyl-acyl carrier protein synthase domain of module 2 of the erythromycin polyketide synthase.
Biochemistry 2004;43(51):16301-10.
2004: Lautaro Diacovich; Deborah Lynn Mitchell; Huy Pham; Gabriela Gago; Melrose Mendoza Melgar; Chaitan Khosla; Hugo Gramajo; Shiou-Chuan Tsai
Crystal structure of the beta-subunit of acyl-CoA carboxylase: structure-based engineering of substrate specificity.
Biochemistry 2004;43(44):14027-36.
2004: Chu-Young Kim; Viktor Y Alekseyev; Alice Y Chen; Yinyan Tang; David E Cane; Chaitan Khosla
Reconstituting modular activity from separated domains of 6-deoxyerythronolide B synthase.
Biochemistry 2004;43(44):13892-8.
2004: Lu Shan; Thomas Marti; Ludvig M Sollid; Gary M Gray; Chaitan Khosla
Comparative biochemical analysis of three bacterial prolyl endopeptidases: implications for coeliac sprue.
The Biochemical journal 2004;383(Pt 2):311-8.
2004: Justin L Piper; Gary M Gray; Chaitan Khosla
Effect of prolyl endopeptidase on digestive-resistant gliadin peptides in vivo.
The Journal of pharmacology and experimental therapeutics 2004;311(1):213-9.
2004: Adrian T Keatinge-Clay; David A Maltby; Katalin F Medzihradszky; Chaitan Khosla; Robert M Stroud
An antibiotic factory caught in action.
Nature structural & molecular biology 2004;11(9):888-93.
2004: Ho Young Lee; Hak Suk Chung; Chao Hang; Chaitan Khosla; Christopher T Walsh; Daniel Kahne; Suzanne Walker
Reconstitution and characterization of a new desosaminyl transferase, EryCIII, from the erythromycin biosynthetic pathway.
Journal of the American Chemical Society 2004;126(32):9924-5.
2004: Shuo-Wang Qiao; Elin Bergseng; Øyvind Molberg; Jiang Xia; Burkhard Fleckenstein; Chaitan Khosla; Ludvig M Sollid
Antigen presentation to celiac lesion-derived T cells of a 33-mer gliadin peptide naturally formed by gastrointestinal digestion.
Journal of immunology (Baltimore, Md. : 1950) 2004;173(3):1757-62.
2004: Yi Tang; Andrew T Koppisch; Chaitan Khosla
The acyltransferase homologue from the initiation module of the R1128 polyketide synthase is an acyl-ACP thioesterase that edits acetyl primer units.
Biochemistry 2004;43(29):9546-55.
2004: Christopher N Boddy; Kinya Hotta; Martha Lovato Tse; R Edward Watts; Chaitan Khosla
Precursor-directed biosynthesis of epothilone in Escherichia coli.
Journal of the American Chemical Society 2004;126(24):7436-7.
2004: Chu-Young Kim; Hanne Quarsten; Elin Bergseng; Chaitan Khosla; Ludvig M Sollid
Structural basis for HLA-DQ2-mediated presentation of gluten epitopes in celiac disease.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(12):4175-9.
2004: Yi Tang; Taek Soon Lee; Chaitan Khosla
Engineered biosynthesis of regioselectively modified aromatic polyketides using bimodular polyketide synthases.
PLoS biology 2004;2(2):E31.
2004: Pawan Kumar; Chaitan Khosla; Yi Tang
Manipulation and analysis of polyketide synthases.
Methods in enzymology 2004;388():269-93.
2003: Chaitan Khosla; Jay D Keasling
Metabolic engineering for drug discovery and development.
Nature reviews. Drug discovery 2003;2(12):1019-25.
2003: Kenji Watanabe; Chaitan Khosla; Robert M Stroud; Shiou-Chuan Tsai
Crystal structure of an Acyl-ACP dehydrogenase from the FK520 polyketide biosynthetic pathway: insights into extender unit biosynthesis.
Journal of molecular biology 2003;334(3):435-44.
2003: Pawan Kumar; Andrew T Koppisch; David E Cane; Chaitan Khosla
Enhancing the modularity of the modular polyketide synthases: transacylation in modular polyketide synthases catalyzed by malonyl-CoA:ACP transacylase.
Journal of the American Chemical Society 2003;125(47):14307-12.
2003: Suzanne J Admiraal; Chaitan Khosla; Christopher T Walsh
A Switch for the transfer of substrate between nonribosomal peptide and polyketide modules of the rifamycin synthetase assembly line.
Journal of the American Chemical Society 2003;125(45):13664-5.
2003: Kenji Kinoshita; Blaine A Pfeifer; Chaitan Khosla; David E Cane
Precursor-Directed polyketide biosynthesis in Escherichia coli.
Bioorganic & medicinal chemistry letters 2003;13(21):3701-4.
2003: Blaine A Pfeifer; Clay C C Wang; Christopher T Walsh; Chaitan Khosla
Biosynthesis of Yersiniabactin, a complex polyketide-nonribosomal peptide, using Escherichia coli as a heterologous host.
Applied and environmental microbiology 2003;69(11):6698-702.
2003: Kenji Watanabe; Clay C C Wang; Christopher N Boddy; David E Cane; Chaitan Khosla
Understanding substrate specificity of polyketide synthase modules by generating hybrid multimodular synthases.
The Journal of biological chemistry 2003;278(43):42020-6.
2003: Yi Tang; Shiou-Chuan Tsai; Chaitan Khosla
Polyketide chain length control by chain length factor.
Journal of the American Chemical Society 2003;125(42):12708-9.
2003: Andrew T Koppisch; Chaitan Khosla
Structure-based mutagenesis of the malonyl-CoA:acyl carrier protein transacylase from Streptomyces coelicolor.
Biochemistry 2003;42(37):11057-64.
2003: Kenji Watanabe; Mathew A Rude; Christopher T Walsh; Chaitan Khosla
Engineered biosynthesis of an ansamycin polyketide precursor in Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(17):9774-8.
2003: Zhihao Hu; Blaine A Pfeifer; Elizabeth Chao; Sumati Murli; James T Kealey; John R Carney; Gary W Ashley; Chaitan Khosla; C Richard Hutchinson
A specific role of the Saccharopolyspora erythraea thioesterase II gene in the function of modular polyketide synthases.
Microbiology (Reading, England) 2003;149(Pt 8):2213-25.
2003: Yi Tang; Taek Soon Lee; Seiji Kobayashi; Chaitan Khosla
Ketosynthases in the initiation and elongation modules of aromatic polyketide synthases have orthogonal acyl carrier protein specificity.
Biochemistry 2003;42(21):6588-95.
2003: Yifeng Yin; Hongxiang Lu; Chaitan Khosla; David E Cane
Expression and kinetic analysis of the substrate specificity of modules 5 and 6 of the picromycin/methymycin polyketide synthase.
Journal of the American Chemical Society 2003;125(19):5671-6.
2003: Marcus Hans; Andreas Hornung; Agnieszka Dziarnowski; David E Cane; Chaitan Khosla
Mechanistic analysis of acyl transferase domain exchange in polyketide synthase modules.
Journal of the American Chemical Society 2003;125(18):5366-74.
2003: Qing Li; Chaitan Khosla; Joseph D Puglisi; Corey W Liu
Solution structure and backbone dynamics of the holo form of the frenolicin acyl carrier protein.
Biochemistry 2003;42(16):4648-57.
2003: Pawan Kumar; Qing Li; David E Cane; Chaitan Khosla
Intermodular communication in modular polyketide synthases: structural and mutational analysis of linker mediated protein-protein recognition.
Journal of the American Chemical Society 2003;125(14):4097-102.
2003: Grace F Liou; Chaitan Khosla
Building-block selectivity of polyketide synthases.
Current opinion in chemical biology 2003;7(2):279-84.
2003: Christopher N Boddy; Tanya L Schneider; Kinya Hotta; Christopher T Walsh; Chaitan Khosla
Epothilone C macrolactonization and hydrolysis are catalyzed by the isolated thioesterase domain of epothilone polyketide synthase.
Journal of the American Chemical Society 2003;125(12):3428-9.
2003: Felix Hausch; Tuula Halttunen; Markku Mäki; Chaitan Khosla
Design, synthesis, and evaluation of gluten peptide analogs as selective inhibitors of human tissue transglutaminase.
Chemistry & biology 2003;10(3):225-31.
2003: Adrian T Keatinge-Clay; Anang A Shelat; David F Savage; Shiou-Chuan Tsai; Larry J W Miercke; Joseph D O'Connell; Chaitan Khosla; Robert M Stroud
Catalysis, specificity, and ACP docking site of Streptomyces coelicolor malonyl-CoA:ACP transacylase.
Structure (London, England : 1993) 2003;11(2):147-54.
2003: Grace F Liou; Janice Lau; David E Cane; Chaitan Khosla
Quantitative analysis of loading and extender acyltransferases of modular polyketide synthases.
Biochemistry 2003;42(1):200-7.
2002: Isabelle Parrot; Philip C Huang; Chaitan Khosla
Circular dichroism and nuclear magnetic resonance spectroscopic analysis of immunogenic gluten peptides and their analogs.
The Journal of biological chemistry 2002;277(47):45572-8.
2002: Hu Pan; Shiou chuan Tsai; Eric S Meadows; Larry J W Miercke; Adrian T Keatinge-Clay; Joe O'Connell; Chaitan Khosla; Robert M Stroud
Crystal structure of the priming beta-ketosynthase from the R1128 polyketide biosynthetic pathway.
Structure (London, England : 1993) 2002;10(11):1559-68.
2002: Shiou-Chuan Tsai; Hongxiang Lu; David E Cane; Chaitan Khosla; Robert M Stroud
Insights into channel architecture and substrate specificity from crystal structures of two macrocycle-forming thioesterases of modular polyketide synthases.
Biochemistry 2002;41(42):12598-606.
2002: Hongxiang Lu; Shiou-Chuan Tsai; Chaitan Khosla; David E Cane
Expression, site-directed mutagenesis, and steady state kinetic analysis of the terminal thioesterase domain of the methymycin/picromycin polyketide synthase.
Biochemistry 2002;41(42):12590-7.
2002: Felix Hausch; Lu Shan; Nilda A Santiago; Gary M Gray; Chaitan Khosla
Intestinal digestive resistance of immunodominant gliadin peptides.
American journal of physiology. Gastrointestinal and liver physiology 2002;283(4):G996-G1003.
2002: Lu Shan; Øyvind Molberg; Isabelle Parrot; Felix Hausch; Ferda Filiz; Gary M Gray; Ludvig M Sollid; Chaitan Khosla
Structural basis for gluten intolerance in celiac sprue.
Science (New York, N.Y.) 2002;297(5590):2275-9.
2002: Jonathan S Dordick; Chaitan Khosla
Engineering of molecular and cellular biocatalysts: selected contributions by James E. Bailey.
Biotechnology and bioengineering 2002;79(5):490-5.
2002: Lautaro Diacovich; Salvador Peirú; Daniel Kurth; Eduardo Rodríguez; Florencio Podestá; Chaitan Khosla; Hugo Gramajo
Kinetic and structural analysis of a new group of Acyl-CoA carboxylases found in Streptomyces coelicolor A3(2).
The Journal of biological chemistry 2002;277(34):31228-36.
2002: Blaine A Pfeifer; Zhihao Hu; Peter Licari; Chaitan Khosla
Process and metabolic strategies for improved production of Escherichia coli-derived 6-deoxyerythronolide B.
Applied and environmental microbiology 2002;68(7):3287-92.
2002: Suzanne J Admiraal; Chaitan Khosla; Christopher T Walsh
The loading and initial elongation modules of rifamycin synthetase collaborate to produce mixed aryl ketide products.
Biochemistry 2002;41(16):5313-24.
2002: Linda C Dayem; John R Carney; Daniel V Santi; Blaine A Pfeifer; Chaitan Khosla; James T Kealey
Metabolic engineering of a methylmalonyl-CoA mutase-epimerase pathway for complex polyketide biosynthesis in Escherichia coli.
Biochemistry 2002;41(16):5193-201.
2002: Nicholas Wu; David E Cane; Chaitan Khosla
Quantitative analysis of the relative contributions of donor acyl carrier proteins, acceptor ketosynthases, and linker regions to intermodular transfer of intermediates in hybrid polyketide synthases.
Biochemistry 2002;41(15):5056-66.
2002: Justin L Piper; Gary M Gray; Chaitan Khosla
High selectivity of human tissue transglutaminase for immunoactive gliadin peptides: implications for celiac sprue.
Biochemistry 2002;41(1):386-93.
2002: David E Cane; Fumitaka Kudo; Kenji Kinoshita; Chaitan Khosla
Precursor-directed biosynthesis: biochemical basis of the remarkable selectivity of the erythromycin polyketide synthase toward unsaturated triketides.
Chemistry & biology 2002;9(1):131-42.
2001: Shiou-Chuan Tsai; Larry J W Miercke; Jolanta Krucinski; R Gokhale; Jeng-Chang Chen; Paul G Foster; D E Cane; Chaitan Khosla; Robert M Stroud
Crystal structure of the macrocycle-forming thioesterase domain of the erythromycin polyketide synthase: versatility from a unique substrate channel.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(26):14808-13.
2001: E S Meadows; Chaitan Khosla
In vitro reconstitution and analysis of the chain initiating enzymes of the R1128 polyketide synthase.
Biochemistry 2001;40(49):14855-61.
2001: J Dreier; Qing Li; Chaitan Khosla
Malonyl-CoA:ACP transacylase from Streptomyces coelicolor has two alternative catalytically active nucleophiles.
Biochemistry 2001;40(41):12407-11.
2001: H T Chiu; B K Hubbard; AN Shah; J Eide; R A Fredenburg; Christopher T Walsh; Chaitan Khosla
Molecular cloning and sequence analysis of the complestatin biosynthetic gene cluster.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(15):8548-53.
2001: Nicholas Wu; S Y Tsuji; David E Cane; Chaitan Khosla
Assessing the balance between protein-protein interactions and enzyme-substrate interactions in the channeling of intermediates between polyketide synthase modules.
Journal of the American Chemical Society 2001;123(27):6465-74.
2001: F Lombó; Blaine A Pfeifer; Timothy Leaf; Sally Ou; Y S Kim; David E Cane; Peter Licari; Chaitan Khosla
Enhancing the atom economy of polyketide biosynthetic processes through metabolic engineering.
Biotechnology progress 2001;17(4):612-7.
2001: NL Pohl; M Hans; Ho Young Lee; Y S Kim; David E Cane; Chaitan Khosla
Remarkably broad substrate tolerance of malonyl-CoA synthetase, an enzyme capable of intracellular synthesis of polyketide precursors.
Journal of the American Chemical Society 2001;123(24):5822-3.
2001: Y Yin; R Gokhale; Chaitan Khosla; David E Cane
Erythromycin biosynthesis. The 4-pro-S hydride of NADPH is utilized for ketoreduction by both module 5 and module 6 of the 6-deoxyerythronolide B synthase.
Bioorganic & medicinal chemistry letters 2001;11(12):1477-9.
2001: SJ Admiraal; Christopher T Walsh; Chaitan Khosla
The loading module of rifamycin synthetase is an adenylation-thiolation didomain with substrate tolerance for substituted benzoates.
Biochemistry 2001;40(20):6116-23.
2001: K Kinoshita; Paul G Williard; Chaitan Khosla; David E Cane
Precursor-directed biosynthesis of 16-membered macrolides by the erythromycin polyketide synthase.
Journal of the American Chemical Society 2001;123(11):2495-502.
2001: Blaine A Pfeifer; SJ Admiraal; H Gramajo; David E Cane; Chaitan Khosla
Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli.
Science (New York, N.Y.) 2001;291(5509):1790-2.
2001: Blaine A Pfeifer; Chaitan Khosla
Biosynthesis of polyketides in heterologous hosts.
Microbiology and molecular biology reviews : MMBR 2001;65(1):106-18.
2001: S Y Tsuji; David E Cane; Chaitan Khosla
Selective protein-protein interactions direct channeling of intermediates between polyketide synthase modules.
Biochemistry 2001;40(8):2326-31.
2001: S Y Tsuji; Nicholas Wu; Chaitan Khosla
Intermodular communication in polyketide synthases: comparing the role of protein-protein interactions to those in other multidomain proteins.
Biochemistry 2001;40(8):2317-25.
2001: AR Salomon; Y Zhang; Haruo Seto; Chaitan Khosla
Structure-activity relationships within a family of selectively cytotoxic macrolide natural products.
Organic letters 2001;3(1):57-9.
2001: Chaitan Khosla; Pehr B Harbury
Modular enzymes.
Nature 2001;409(6817):247-52.
2001: AR Salomon; David Voehringer; Leonore A Herzenberg; Chaitan Khosla
Apoptolidin, a selective cytotoxic agent, is an inhibitor of F0F1-ATPase.
Chemistry & biology 2001;8(1):71-80.
2000: AR Salomon; David Voehringer; Leonore A Herzenberg; Chaitan Khosla
Understanding and exploiting the mechanistic basis for selectivity of polyketide inhibitors of F(0)F(1)-ATPase.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(26):14766-71.
2000: Chaitan Khosla
Natural product biosynthesis: a new interface between enzymology and medicine.
The Journal of organic chemistry 2000;65(24):8127-33.
2000: T Marti; Z Hu; NL Pohl; AN Shah; Chaitan Khosla
Cloning, nucleotide sequence, and heterologous expression of the biosynthetic gene cluster for R1128, a non-steroidal estrogen receptor antagonist. Insights into an unusual priming mechanism.
The Journal of biological chemistry 2000;275(43):33443-8.
2000: Janice Lau; David E Cane; Chaitan Khosla
Substrate specificity of the loading didomain of the erythromycin polyketide synthase.
Biochemistry 2000;39(34):10514-20.
2000: Zhihao Hu; D A Hopwood; Chaitan Khosla
Directed transfer of large DNA fragments between Streptomyces species.
Applied and environmental microbiology 2000;66(5):2274-7.
2000: J Dreier; Chaitan Khosla
Mechanistic analysis of a type II polyketide synthase. Role of conserved residues in the beta-ketoacyl synthase-chain length factor heterodimer.
Biochemistry 2000;39(8):2088-95.
2000: RS Gokhale; Chaitan Khosla
Role of linkers in communication between protein modules.
Current opinion in chemical biology 2000;4(1):22-7.
1999: Zhihao Hu; Daniel Hunziker; C R Hutchinson; Chaitan Khosla
A host-vector system for analysis and manipulation of rifamycin polyketide biosynthesis in Amycolatopsis mediterranei.
Microbiology (Reading, England) 1999;145 ( Pt 9)():2335-41.
1999: R J Zawada; Chaitan Khosla
Heterologous expression, purification, reconstitution and kinetic analysis of an extended type II polyketide synthase.
Chemistry & biology 1999;6(9):607-15.
1999: J Dreier; AN Shah; Chaitan Khosla
Kinetic analysis of the actinorhodin aromatic polyketide synthase.
The Journal of biological chemistry 1999;274(35):25108-12.
1999: RS Gokhale; S Y Tsuji; David E Cane; Chaitan Khosla
Dissecting and exploiting intermodular communication in polyketide synthases.
Science (New York, N.Y.) 1999;284(5413):482-5.
1999: M T Richardson; NL Pohl; James T Kealey; Chaitan Khosla
Tolerance and specificity of recombinant 6-methylsalicyclic acid synthase.
Metabolic engineering 1999;1(2):180-7.
1999: Janice Lau; H Fu; David E Cane; Chaitan Khosla
Dissecting the role of acyltransferase domains of modular polyketide synthases in the choice and stereochemical fate of extender units.
Biochemistry 1999;38(5):1643-51.
1999: RS Gokhale; Daniel Hunziker; David E Cane; Chaitan Khosla
Mechanism and specificity of the terminal thioesterase domain from the erythromycin polyketide synthase.
Chemistry & biology 1999;6(2):117-25.
1999: Chaitan Khosla; RS Gokhale; JR Jacobsen; David E Cane
Tolerance and specificity of polyketide synthases.
Annual review of biochemistry 1999;68():219-53.
1998: David E Cane; C T Walsh; Chaitan Khosla
Harnessing the biosynthetic code: combinations, permutations, and mutations.
Science (New York, N.Y.) 1998;282(5386):63-8.
1998: JR Jacobsen; A T Keatinge-Clay; David E Cane; Chaitan Khosla
Precursor-directed biosynthesis of 12-ethyl erythromycin.
Bioorganic & medicinal chemistry 1998;6(8):1171-7.
1998: JR Jacobsen; David E Cane; Chaitan Khosla
Spontaneous priming of a downstream module in 6-deoxyerythronolide B synthase leads to polyketide biosynthesis.
Biochemistry 1998;37(14):4928-34.
1998: C W Carreras; Chaitan Khosla
Purification and in vitro reconstitution of the essential protein components of an aromatic polyketide synthase.
Biochemistry 1998;37(8):2084-8.
1998: RS Gokhale; Janice Lau; David E Cane; Chaitan Khosla
Functional orientation of the acyltransferase domain in a module of the erythromycin polyketide synthase.
Biochemistry 1998;37(8):2524-8.
1998: JR Jacobsen; Chaitan Khosla
New directions in metabolic engineering.
Current opinion in chemical biology 1998;2(1):133-7.
1997: J A Chuck; M McPherson; H Huang; JR Jacobsen; Chaitan Khosla; David E Cane
Molecular recognition of diketide substrates by a beta-ketoacyl-acyl carrier protein synthase domain within a bimodular polyketide synthase.
Chemistry & biology 1997;4(10):757-66.
1997: C W Carreras; A M Gehring; Christopher T Walsh; Chaitan Khosla
Utilization of enzymatically phosphopantetheinylated acyl carrier proteins and acetyl-acyl carrier proteins by the actinorhodin polyketide synthase.
Biochemistry 1997;36(39):11757-61.
1997: R McDaniel; C M Kao; S J Hwang; Chaitan Khosla
Engineered intermodular and intramodular polyketide synthase fusions.
Chemistry & biology 1997;4(9):667-74.
1997: JR Jacobsen; C Richard Hutchinson; David E Cane; Chaitan Khosla
Precursor-directed biosynthesis of erythromycin analogs by an engineered polyketide synthase.
Science (New York, N.Y.) 1997;277(5324):367-9.
1997: R J Zawada; Chaitan Khosla
Domain analysis of the molecular recognition features of aromatic polyketide synthase subunits.
The Journal of biological chemistry 1997;272(26):16184-8.
1997: Rembert Pieper; RS Gokhale; G Luo; David E Cane; Chaitan Khosla
Purification and characterization of bimodular and trimodular derivatives of the erythromycin polyketide synthase.
Biochemistry 1997;36(7):1846-51.
1996: N Tsukamoto; J A Chuck; G Luo; C M Kao; Chaitan Khosla; David E Cane
6-deoxyerythronolide B synthase 1 is specifically acylated by a diketide intermediate at the beta-ketoacyl-acyl carrier protein synthase domain of module 2.
Biochemistry 1996;35(48):15244-8.
1996: R H Lambalot; AM Gehring; R S Flugel; Peter Zuber; M LaCelle; Mohamed A Marahiel; R Reid; Chaitan Khosla; Christopher T Walsh
A new enzyme superfamily - the phosphopantetheinyl transferases.
Chemistry & biology 1996;3(11):923-36.
1996: P J Kramer; Chaitan Khosla
Engineering of novel polyketides. Progress and Prospects.
Annals of the New York Academy of Sciences 1996;799():32-45.
1996: Chaitan Khosla; R Caren; C M Kao; R McDaniel; S W Wang
Evolutionally guided enzyme design.
Biotechnology and bioengineering 1996;52(1):122-8.
1996: D Bedford; JR Jacobsen; G Luo; David E Cane; Chaitan Khosla
A functional chimeric modular polyketide synthase generated via domain replacement.
Chemistry & biology 1996;3(10):827-31.
1996: C M Kao; Rembert Pieper; David E Cane; Chaitan Khosla
Evidence for two catalytically independent clusters of active sites in a functional modular polyketide synthase.
Biochemistry 1996;35(38):12363-8.
1996: Chaitan Khosla; R J Zawada
Generation of polyketide libraries via combinatorial biosynthesis.
Trends in biotechnology 1996;14(9):335-41.
1996: G Luo; Rembert Pieper; A Rosa; Chaitan Khosla; David E Cane
Erythromycin biosynthesis: exploiting the catalytic versatility of the modular polyketide synthase.
Bioorganic & medicinal chemistry 1996;4(7):995-9.
1996: H Fu; M A Alvarez; Chaitan Khosla; J E Bailey
Engineered biosynthesis of novel polyketides: regiospecific methylation of an unnatural substrate by the tcmO O-methyltransferase.
Biochemistry 1996;35(21):6527-32.
1996: Rembert Pieper; S Ebert-Khosla; David E Cane; Chaitan Khosla
Erythromycin biosynthesis: kinetic studies on a fully active modular polyketide synthase using natural and unnatural substrates.
Biochemistry 1996;35(7):2054-60.
1995: Rembert Pieper; G Luo; David E Cane; Chaitan Khosla
Cell-free synthesis of polyketides by recombinant erythromycin polyketide synthases.
Nature 1995;378(6554):263-6.
1995: D J Bedford; E Schweizer; David A Hopwood; Chaitan Khosla
Expression of a functional fungal polyketide synthase in the bacterium Streptomyces coelicolor A3(2).
Journal of bacteriology 1995;177(15):4544-8.
1995: David E Cane; G Luo; Chaitan Khosla; C M Kao; L Katz
Erythromycin biosynthesis. Highly efficient incorporation of polyketide chain elongation intermediates into 6-deoxyerythronolide B in an engineered Streptomyces host.
The Journal of antibiotics 1995;48(7):647-51.
1995: R McDaniel; S Ebert-Khosla; David A Hopwood; Chaitan Khosla
Rational design of aromatic polyketide natural products by recombinant assembly of enzymatic subunits.
Nature 1995;375(6532):549-54.
1995: C J Tsoi; Chaitan Khosla
Combinatorial biosynthesis of 'unnatural' natural products: the polyketide example.
Chemistry & biology 1995;2(6):355-62.
1994: H Fu; David A Hopwood; Chaitan Khosla
Engineered biosynthesis of novel polyketides: evidence for temporal, but not regiospecific, control of cyclization of an aromatic polyketide precursor.
Chemistry & biology 1994;1(4):205-10.
1994: R McDaniel; S Ebert-Khosla; H Fu; David A Hopwood; Chaitan Khosla
Engineered biosynthesis of novel polyketides: influence of a downstream enzyme on the catalytic specificity of a minimal aromatic polyketide synthase.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(24):11542-6.
1994: H Fu; R McDaniel; David A Hopwood; Chaitan Khosla
Engineered biosynthesis of novel polyketides: stereochemical course of two reactions catalyzed by a polyketide synthase.
Biochemistry 1994;33(31):9321-6.
1994: C M Kao; L Katz; Chaitan Khosla
Engineered biosynthesis of a complete macrolactone in a heterologous host.
Science (New York, N.Y.) 1994;265(5171):509-12.
1993: R McDaniel; S Ebert-Khosla; David A Hopwood; Chaitan Khosla
Engineered biosynthesis of novel polyketides.
Science (New York, N.Y.) 1993;262(5139):1546-50.
1993: Chaitan Khosla; R McDaniel; S Ebert-Khosla; R Torres; David H Sherman; Maureen J Bibb; David A Hopwood
Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins.
Journal of bacteriology 1993;175(8):2197-204.
1992: Chaitan Khosla; S Ebert-Khosla; David A Hopwood
Targeted gene replacements in a Streptomyces polyketide synthase gene cluster: role for the acyl carrier protein.
Molecular microbiology 1992;6(21):3237-49.
1992: David A Hopwood; Chaitan Khosla
Genes for polyketide secondary metabolic pathways in microorganisms and plants.
Ciba Foundation symposium 1992;171():88-106; discussion 106-12.