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Douglas Kilburn

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Mojgan Kavoosi; A Louise Creagh; Robin F B Turner; Douglas G Kilburn; Charles A Haynes
Direct measurement of the kinetics of CBM9 fusion-tag bioprocessing using luminescence resonance energy transfer.
Mojgan Kavoosi; Dexter Lam; Jenny Bryan; Douglas G Kilburn; Charles A Haynes
Mechanically stable porous cellulose media for affinity purification of family 9 cellulose-binding module-tagged fusion proteins.
Mojgan Kavoosi; A Louise Creagh; Douglas G Kilburn; Charles A Haynes
Strategy for selecting and characterizing linker peptides for CBM9-tagged fusion proteins expressed in Escherichia coli.

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All Publications

2009: Mojgan Kavoosi; A Louise Creagh; Robin F B Turner; Douglas G Kilburn; Charles A Haynes
Direct measurement of the kinetics of CBM9 fusion-tag bioprocessing using luminescence resonance energy transfer.
Biotechnology progress 2009;25(3):874-81.
2007: Mojgan Kavoosi; Dexter Lam; Jenny Bryan; Douglas G Kilburn; Charles A Haynes
Mechanically stable porous cellulose media for affinity purification of family 9 cellulose-binding module-tagged fusion proteins.
Journal of chromatography. A 2007;1175(2):187-96.
2007: Mojgan Kavoosi; A Louise Creagh; Douglas G Kilburn; Charles A Haynes
Strategy for selecting and characterizing linker peptides for CBM9-tagged fusion proteins expressed in Escherichia coli.
Biotechnology and bioengineering 2007;98(3):599-610.
2007: Mojgan Kavoosi; Nooshafarin Sanaie; Florian Dismer; Jürgen Hubbuch; Douglas G Kilburn; Charles A Haynes
A novel two-zone protein uptake model for affinity chromatography and its application to the description of elution band profiles of proteins fused to a family 9 cellulose binding module affinity tag.
Journal of chromatography. A 2007;1160(1-2):137-49.
2006: Alex Berlin; Neil Gilkes; Douglas G Kilburn; Vera Maximenko; Renata Bura; Alexander V Markov; Anton Skomarovsky; Alexander Gusakov; Arkady Sinitsyn; Oleg Okunev; Irina Solovieva; John N Saddler
Evaluation of cellulase preparations for hydrolysis of hardwood substrates.
Applied biochemistry and biotechnology 2006;129-132():528-45.
2005: Emily M Kwan; Alisdair B Boraston; Bradley W McLean; Douglas G Kilburn; R Antony J Warren
N-Glycosidase-carbohydrate-binding module fusion proteins as immobilized enzymes for protein deglycosylation.
Protein engineering, design & selection : PEDS 2005;18(10):497-501.
2005: Eric J Jervis; M Marta Guarna; James Doheny; Charles A Haynes; Douglas G Kilburn
Dynamic localization and persistent stimulation of factor-dependent cells by a stem cell factor / cellulose binding domain fusion protein.
Biotechnology and bioengineering 2005;91(3):314-24.
2005: Arwa Kurabi; Alex Berlin; Neil Gilkes; Douglas G Kilburn; Renata Bura; Jamie Robinson; Alexander V Markov; Anton Skomarovsky; Aleksandr Gusakov; Oleg Okunev; Arkady Sinitsyn; David J Gregg; Dan Xie; John N Saddler
Enzymatic hydrolysis of steam-exploded and ethanol organosolv-pretreated Douglas-Firby novel and commercial fungal cellulases.
Applied biochemistry and biotechnology 2005;121-124():219-30.
2005: Alex Berlin; Neil Gilkes; Arwa Kurabi; Renata Bura; Maobing Tu; Douglas G Kilburn; John N Saddler
Weak lignin-binding enzymes: a novel approach to improve activity of cellulases for hydrolysis of lignocellulosics.
Applied biochemistry and biotechnology 2005;121-124():163-70.
2004: Beatriz Rodriguez; Mojgan Kavoosi; Jürgen Koska; A Louise Creagh; Douglas G Kilburn; Charles A Haynes
Inexpensive and generic affinity purification of recombinant proteins using a family 2a CBM fusion tag.
Biotechnology progress 2004;20(5):1479-89.
2004: Mojgan Kavoosi; Julia Meijer; Emily M Kwan; A Louise Creagh; Douglas G Kilburn; Charles A Haynes
Inexpensive one-step purification of polypeptides expressed in Escherichia coli as fusions with the family 9 carbohydrate-binding module of xylanase 10A from T. maritima.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2004;807(1):87-94.
2004: Volker M Gorenflo; Tom A Pfeifer; Gary Lesnicki; Emily M Kwan; Thomas A Grigliatti; Douglas G Kilburn; James M Piret
Production of a self-activating CBM-factor X fusion protein in a stable transformed Sf9 insect cell line using high cell density perfusion culture.
Cytotechnology 2004;44(3):93-102.
2003: Alisdair B Boraston; Valerie Notenboom; R Antony J Warren; Douglas G Kilburn; David R Rose; Gideon Davies
Structure and ligand binding of carbohydrate-binding module CsCBM6-3 reveals similarities with fucose-specific lectins and "galactose-binding" domains.
Journal of molecular biology 2003;327(3):659-69.
2003: Alisdair B Boraston; Emily M Kwan; Patrick Chiu; R Antony J Warren; Douglas G Kilburn
Recognition and hydrolysis of noncrystalline cellulose.
The Journal of biological chemistry 2003;278(8):6120-7.
2003: Brian E Mayson; Douglas G Kilburn; Bruce L Zamost; Christopher K Raymond; Gary J Lesnicki
Effects of methanol concentration on expression levels of recombinant protein in fed-batch cultures of Pichia methanolica.
Biotechnology and bioengineering 2003;81(3):291-8.
2003: Alisdair B Boraston; Linda E Sandercock; R Antony J Warren; Douglas G Kilburn
O-glycosylation of a recombinant carbohydrate-binding module mutant secreted by Pichia pastoris.
Journal of molecular microbiology and biotechnology 2003;5(1):29-36.
2002: Bradley W McLean; Alisdair B Boraston; Darren Brouwer; Nooshafarin Sanaie; Colin A Fyfe; R Antony J Warren; Douglas G Kilburn; Charles A Haynes
Carbohydrate-binding modules recognize fine substructures of cellulose.
The Journal of biological chemistry 2002;277(52):50245-54.
2002: Emily M Kwan; M Marta Guarna; Alisdair B Boraston; Neil Gilkes; Charles A Haynes; Douglas G Kilburn; R Antony J Warren
Self-activating factor X derivative fused to the C-terminus of a cellulose-binding module: Production and properties.
Biotechnology and bioengineering 2002;79(7):724-32.
2002: Alisdair B Boraston; Didier Nurizzo; Valerie Notenboom; Valérie M-A Ducros; David R Rose; Douglas G Kilburn; Gideon J Davies
Differential oligosaccharide recognition by evolutionarily-related beta-1,4 and beta-1,3 glucan-binding modules.
Journal of molecular biology 2002;319(5):1143-56.
2002: Valerie Notenboom; Alisdair B Boraston; Spencer J Williams; Douglas G Kilburn; David R Rose
High-resolution crystal structures of the lectin-like xylan binding domain from Streptomyces lividans xylanase 10A with bound substrates reveal a novel mode of xylan binding.
Biochemistry 2002;41(13):4246-54.
2002: Alisdair B Boraston; Bradley W McLean; Grace Chen; Anson Li; R Antony J Warren; Douglas G Kilburn
Co-operative binding of triplicate carbohydrate-binding modules from a thermophilic xylanase.
Molecular microbiology 2002;43(1):187-94.
2002: Alisdair B Boraston; Mazyar Ghaffari; R Antony J Warren; Douglas G Kilburn
Identification and glucan-binding properties of a new carbohydrate-binding module family.
The Biochemical journal 2002;361(Pt 1):35-40.
2001: Valerie Notenboom; Alisdair B Boraston; Patrick Chiu; A C Freelove; Douglas G Kilburn; David R Rose
Recognition of cello-oligosaccharides by a family 17 carbohydrate-binding module: an X-ray crystallographic, thermodynamic and mutagenic study.
Journal of molecular biology 2001;314(4):797-806.
2001: Alisdair B Boraston; R Antony J Warren; Douglas G Kilburn
beta-1,3-Glucan binding by a thermostable carbohydrate-binding module from Thermotoga maritima.
Biochemistry 2001;40(48):14679-85.
2001: Tom A Pfeifer; M Marta Guarna; Emily M Kwan; G Lesnicki; David A Theilmann; Thomas A Grigliatti; Douglas G Kilburn
Expression analysis of a modified factor X in stably transformed insect cell lines.
Protein expression and purification 2001;23(2):233-41.
2001: Alisdair B Boraston; R Antony J Warren; Douglas G Kilburn
Glycosylation by Pichia pastoris decreases the affinity of a family 2a carbohydrate-binding module from Cellulomonas fimi: a functional and mutational analysis.
The Biochemical journal 2001;358(Pt 2):423-30.
2001: Valerie Notenboom; Alisdair B Boraston; Douglas G Kilburn; David R Rose
Crystal structures of the family 9 carbohydrate-binding module from Thermotoga maritima xylanase 10A in native and ligand-bound forms.
Biochemistry 2001;40(21):6248-56.
2001: Alisdair B Boraston; A Louise Creagh; M M Alam; JM Kormos; P Tomme; Charles A Haynes; R Antony J Warren; Douglas G Kilburn
Binding specificity and thermodynamics of a family 9 carbohydrate-binding module from Thermotoga maritima xylanase 10A.
Biochemistry 2001;40(21):6240-7.
2001: Alisdair B Boraston; Bradley W McLean; M Marta Guarna; E Amandaron-Akow; Douglas G Kilburn
A family 2a carbohydrate-binding module suitable as an affinity tag for proteins produced in Pichia pastoris.
Protein expression and purification 2001;21(3):417-23.
2001: Ali R Esteghlalian; V Srivastava; Neil Gilkes; Douglas G Kilburn; R Antony J Warren; J N Saddle
Do cellulose binding domains increase substrate accessibility?
Applied biochemistry and biotechnology 2001;91-93():575-92.
2000: Bradley W McLean; M R Bray; Alisdair B Boraston; Neil Gilkes; Charles A Haynes; Douglas G Kilburn
Analysis of binding of the family 2a carbohydrate-binding module from Cellulomonas fimi xylanase 10A to cellulose: specificity and identification of functionally important amino acid residues.
Protein engineering 2000;13(11):801-9.
2000: M Marta Guarna; Hélène C F Côté; Emily M Kwan; Gordon L Rintoul; B Meyhack; Jutta Heim; Ross T A MacGillivray; R Antony J Warren; Douglas G Kilburn
Factor X fusion proteins: improved production and use in the release in vitro of biologically active hirudin from an inactive alpha-factor-hirudin fusion protein.
Protein expression and purification 2000;20(2):133-41.
2000: Alisdair B Boraston; P Tomme; E A Amandoron; Douglas G Kilburn
A novel mechanism of xylan binding by a lectin-like module from Streptomyces lividans xylanase 10A.
The Biochemical journal 2000;350 Pt 3():933-41.
2000: Alisdair B Boraston; Patrick Chiu; R Antony J Warren; Douglas G Kilburn
Specificity and affinity of substrate binding by a family 17 carbohydrate-binding module from Clostridium cellulovorans cellulase 5A.
Biochemistry 2000;39(36):11129-36.
2000: JM Kormos; PE Johnson; E Brun; P Tomme; Lawrence P McIntosh; Charles A Haynes; Douglas G Kilburn
Binding site analysis of cellulose binding domain CBD(N1) from endoglucanse C of Cellulomonas fimi by site-directed mutagenesis.
Biochemistry 2000;39(30):8844-52.
2000: Dominik Stoll; Alisdair B Boraston; Henrik Stålbrand; Bradley W McLean; Douglas G Kilburn; R Antony J Warren
Mannanase Man26A from Cellulomonas fimi has a mannan-binding module.
FEMS microbiology letters 2000;183(2):265-9.
1999: PW Zandstra; Eric J Jervis; Charles A Haynes; Douglas G Kilburn; Connie J Eaves; James M Piret
Concentration-dependent internalization of a cytokine/cytokine receptor complex in human hematopoietic cells.
Biotechnology and bioengineering 1999;63(4):493-501.
1999: C H Fann; M Marta Guarna; Douglas G Kilburn; James M Piret
Relationship between recombinant activated protein C secretion rates and mRNA levels in baby hamster kidney cells.
Biotechnology and bioengineering 1999;63(4):464-72.
1999: James Doheny; Eric J Jervis; M Marta Guarna; R Keith Humphries; R Antony J Warren; Douglas G Kilburn
Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor-cellulose-binding domain fusion protein.
The Biochemical journal 1999;339 ( Pt 2)():429-34.
1998: P Tomme; Alisdair B Boraston; Bradley W McLean; JM Kormos; A Louise Creagh; K Sturch; Neil Gilkes; Charles A Haynes; R Antony J Warren; Douglas G Kilburn
Characterization and affinity applications of cellulose-binding domains.
Journal of chromatography. B, Biomedical sciences and applications 1998;715(1):283-96.
1998: Henrik Stålbrand; Shawn D Mansfield; John N Saddler; Douglas G Kilburn; R Antony J Warren; Neil Gilkes
Analysis of molecular size distributions of cellulose molecules during hydrolysis of cellulose by recombinant Cellulomonas fimi beta-1,4-glucanases.
Applied and environmental microbiology 1998;64(7):2374-9.
1998: A Louise Creagh; Jürgen Koska; PE Johnson; P Tomme; MD Joshi; Lawrence P McIntosh; Douglas G Kilburn; Charles A Haynes
Stability and oligosaccharide binding of the N1 cellulose-binding domain of Cellulomonas fimi endoglucanase CenC.
Biochemistry 1998;37(10):3529-37.
1997: M Marta Guarna; G J Lesnicki; B M Tam; J Robinson; C Z Radziminski; D Hasenwinkle; Alisdair B Boraston; Eric J Jervis; Ross T A MacGillivray; Robin F B Turner; Douglas G Kilburn
On-line monitoring and control of methanol concentration in shake-flask cultures of Pichia pastoris.
Biotechnology and bioengineering 1997;56(3):279-86.
1997: D Hasenwinkle; Eric J Jervis; O Kops; C Liu; G Lesnicki; Charles A Haynes; Douglas G Kilburn
Very high-level production and export in Escherichia coli of a cellulose binding domain for use in a generic secretion-affinity fusion system.
Biotechnology and bioengineering 1997;55(6):854-63.
1997: Eric J Jervis; Charles A Haynes; Douglas G Kilburn
Surface diffusion of cellulases and their isolated binding domains on cellulose.
The Journal of biological chemistry 1997;272(38):24016-23.
1996: PE Johnson; MD Joshi; P Tomme; Douglas G Kilburn; Lawrence P McIntosh
Structure of the N-terminal cellulose-binding domain of Cellulomonas fimi CenC determined by nuclear magnetic resonance spectroscopy.
Biochemistry 1996;35(45):14381-94.
1996: P Tomme; A Louise Creagh; Douglas G Kilburn; Charles A Haynes
Interaction of polysaccharides with the N-terminal cellulose-binding domain of Cellulomonas fimi CenC. 1. Binding specificity and calorimetric analysis.
Biochemistry 1996;35(44):13885-94.
1996: A Louise Creagh; E Ong; Eric J Jervis; Douglas G Kilburn; Charles A Haynes
Binding of the cellulose-binding domain of exoglucanase Cex from Cellulomonas fimi to insoluble microcrystalline cellulose is entropically driven.
Proceedings of the National Academy of Sciences of the United States of America 1996;93(22):12229-34.
1996: P Tomme; Neil Gilkes; M Marta Guarna; Charles A Haynes; D Hasenwinkle; Eric J Jervis; P Johnson; L McIntosh; R Antony J Warren; Douglas G Kilburn
Cellulose-binding domains. Versatile affinity tags for inexpensive large-scale purification, concentration, and immobilization of fusion proteins.
Annals of the New York Academy of Sciences 1996;799():418-24.
1996: M Marta Guarna; Hélène C F Côté; E A Amandoron; Ross T A MacGillivray; R Antony J Warren; Douglas G Kilburn
Engineering factor X fusions for expression in Pichia pastoris.
Annals of the New York Academy of Sciences 1996;799():397-400.
1996: P Tomme; E Kwan; Neil Gilkes; Douglas G Kilburn; R Antony J Warren
Characterization of CenC, an enzyme from Cellulomonas fimi with both endo- and exoglucanase activities.
Journal of bacteriology 1996;178(14):4216-23.
1996: Eric J Jervis; Douglas G Kilburn
Strategies for maximizing metallothionein promoter regulated recombinant protein production in mammalian cell cultures.
Cytotechnology 1996;21(3):217-23.
1995: H Shen; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi.
The Biochemical journal 1995;311 ( Pt 1)():67-74.
1995: P Tomme; D P Driver; E A Amandoron; R C Miller; R Antony; J Warren; Douglas G Kilburn
Comparison of a fungal (family I) and bacterial (family II) cellulose-binding domain.
Journal of bacteriology 1995;177(15):4356-63.
1995: G Y Xu; E Ong; Neil Gilkes; Douglas G Kilburn; D Ranjith Muhandiram; Marees Harris-Brandts; J P Carver; Lewis E Kay; T S Harvey
Solution structure of a cellulose-binding domain from Cellulomonas fimi by nuclear magnetic resonance spectroscopy.
Biochemistry 1995;34(21):6993-7009.
1995: R W Graham; J M Greenwood; R Antony J Warren; Douglas G Kilburn; D E Trimbur
The pTugA and pTugAS vectors for high-level expression of cloned genes in Escherichia coli.
Gene 1995;158(1):51-4.
1995: E Ong; J B Alimonti; J M Greenwood; R C Miller; R Antony J Warren; Douglas G Kilburn
Purification of human interleukin-2 using the cellulose-binding domain of a prokaryotic cellulase.
Bioseparation 1995;5(2):95-104.
1995: A Meinke; H G Damude; P Tomme; E Kwan; Douglas G Kilburn; R C Miller; R Antony J Warren; Neil Gilkes
Enhancement of the endo-beta-1,4-glucanase activity of an exocellobiohydrolase by deletion of a surface loop.
The Journal of biological chemistry 1995;270(9):4383-6.
1995: P W Pui; F Trampler; S A Sonderhoff; M Groeschl; Douglas G Kilburn; James M Piret
Batch and semicontinuous aggregation and sedimentation of hybridoma cells by acoustic resonance fields.
Biotechnology progress 1995;11(2):146-52.
1995: H G Damude; Stephen G Withers; Douglas G Kilburn; R C Miller; R Antony J Warren
Site-directed mutation of the putative catalytic residues of endoglucanase CenA from Cellulomonas fimi.
Biochemistry 1995;34(7):2220-4.
1995: Z Assouline; R Graham; R C Miller; A J Warren; Douglas G Kilburn
Processing of fusion proteins with immobilized factor Xa.
Biotechnology progress 1995;11(1):45-9.
1994: N Din; H G Damude; Neil Gilkes; R C Miller; R Antony J Warren; Douglas G Kilburn
C1-Cx revisited: intramolecular synergism in a cellulase.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(24):11383-7.
1994: K D Le; Neil Gilkes; Douglas G Kilburn; R C Miller; John N Saddler; R Antony J Warren
A streptavidin-cellulose-binding domain fusion protein that binds biotinylated proteins to cellulose.
Enzyme and microbial technology 1994;16(6):496-500.
1994: A Meinke; Neil Gilkes; E Kwan; Douglas G Kilburn; R Antony J Warren; R C Miller
Cellobiohydrolase A (CbhA) from the cellulolytic bacterium Cellulomonas fimi is a beta-1,4-exocellobiohydrolase analogous to Trichoderma reesei CBH II.
Molecular microbiology 1994;12(3):413-22.
1994: H Shen; P Tomme; A Meinke; Neil Gilkes; Douglas G Kilburn; R Antony J Warren; R C Miller
Stereochemical course of hydrolysis catalysed by Cellulomonas fimi CenE, a member of a new family of beta-1,4-glucanases.
Biochemical and biophysical research communications 1994;199(3):1223-8.
1994: F Trampler; S A Sonderhoff; P W Pui; Douglas G Kilburn; James M Piret
Acoustic cell filter for high density perfusion culture of hybridoma cells.
Bio/technology (Nature Publishing Company) 1994;12(3):281-4.
1994: N Din; I J Forsythe; Leslie D Burtnick; Neil Gilkes; R C Miller; R Antony J Warren; Douglas G Kilburn
The cellulose-binding domain of endoglucanase A (CenA) from Cellulomonas fimi: evidence for the involvement of tryptophan residues in binding.
Molecular microbiology 1994;11(4):747-55.
1994: E Ong; Douglas G Kilburn; R C Miller; R Antony J Warren
Streptomyces lividans glycosylates the linker region of a beta-1,4-glycanase from Cellulomonas fimi.
Journal of bacteriology 1994;176(4):999-1008.
1994: P Tomme; Neil Gilkes; R C Miller; A J Warren; Douglas G Kilburn
An internal cellulose-binding domain mediates adsorption of an engineered bifunctional xylanase/cellulase.
Protein engineering 1994;7(1):117-23.
1993: Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren; Junji Sugiyama; Henri Chanzy; Bernard Henrissat
Visualization of the adsorption of a bacterial endo-beta-1,4-glucanase and its isolated cellulose-binding domain to crystalline cellulose.
International journal of biological macromolecules 1993;15(6):347-51.
1993: C Ramírez; J Fung; R C Miller; R Antony; J Warren; Douglas G Kilburn
A bifunctional affinity linker to couple antibodies to cellulose.
Bio/technology (Nature Publishing Company) 1993;11(13):1570-3.
1993: R W Graham; T Atkinson; Douglas G Kilburn; R C Miller; R Antony J Warren
Rational design and PCR-based synthesis of an artificial Schizophyllum commune xylanase gene.
Nucleic acids research 1993;21(21):4923-8.
1993: Z Assouline; H Shen; Douglas G Kilburn; R Antony J Warren
Production and properties of a factor X-cellulose-binding domain fusion protein.
Protein engineering 1993;6(7):787-92.
1993: A Meinke; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase.
Journal of bacteriology 1993;175(7):1910-8.
1993: J A Francisco; C Stathopoulos; R A Warren; Douglas G Kilburn; george Georgiou
Specific adhesion and hydrolysis of cellulose by intact Escherichia coli expressing surface anchored cellulase or cellulose binding domains.
Bio/technology (Nature Publishing Company) 1993;11(4):491-5.
1993: H G Damude; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Endoglucanase CasA from alkalophilic Streptomyces strain KSM-9 is a typical member of family B of beta-1,4-glucanases.
Gene 1993;123(1):105-7.
1992: A M MacLeod; Neil Gilkes; L Escote-Carlson; R Antony J Warren; Douglas G Kilburn; R C Miller
Streptomyces lividans glycosylates an exoglucanase (Cex) from Cellulomonas fimi.
Gene 1992;121(1):143-7.
1992: D W Lee; James M Piret; D Gregory; D J Haddow; Douglas G Kilburn
Polystyrene macroporous bead support for mammalian cell culture.
Annals of the New York Academy of Sciences 1992;665():137-45.
1992: A Meinke; M Schmuck; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
The tertiary structure of endo-beta-1,4-glucanase B (CenB), a multidomain cellulase from the bacterium Cellulomonas fimi.
Glycobiology 1992;2(4):321-6.
1992: J Gebler; Neil Gilkes; Marc Claeyssens; D B Wilson; Pierre Béguin; Warren W Wakarchuk; Douglas G Kilburn; R C Miller; R Antony J Warren; Stephen G Withers
Stereoselective hydrolysis catalyzed by related beta-1,4-glucanases and beta-1,4-xylanases.
The Journal of biological chemistry 1992;267(18):12559-61.
1992: J M Greenwood; E Ong; Neil Gilkes; R Antony J Warren; R C Miller; Douglas G Kilburn
Cellulose-binding domains: potential for purification of complex proteins.
Protein engineering 1992;5(4):361-5.
1992: J B Coutinho; Neil Gilkes; R Antony J Warren; Douglas G Kilburn; R C Miller
The binding of Cellulomonas fimi endoglucanase C (CenC) to cellulose and Sephadex is mediated by the N-terminal repeats.
Molecular microbiology 1992;6(9):1243-52.
1992: P B Miller; H Shen; Neil Gilkes; Douglas G Kilburn; R C Miller; A G Plaut; R Antony J Warren
Endoglucanase A from Cellulomonas fimi in which the hinge sequence of human IgA1 is substituted for the linker connecting its two domains is hydrolyzed by IgA proteases from Neisseria gonorrhoeae.
FEMS microbiology letters 1992;71(2):199-203.
1992: Neil Gilkes; Eric J Jervis; Bernard Henrissat; B Tekant; R C Miller; R Antony J Warren; Douglas G Kilburn
The adsorption of a bacterial cellulase and its two isolated domains to crystalline cellulose.
The Journal of biological chemistry 1992;267(10):6743-9.
1991: Neil Gilkes; Marc Claeyssens; Ruedi Aebersold; Bernard Henrissat; A Meinke; H D Morrison; Douglas G Kilburn; R Antony J Warren; R C Miller
Structural and functional relationships in two families of beta-1,4-glycanases.
European journal of biochemistry / FEBS 1991;202(2):367-77.
1991: A Meinke; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Bacterial cellulose-binding domain-like sequences in eucaryotic polypeptides.
Protein sequences & data analysis 1991;4(6):349-53.
1991: A Meinke; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Multiple domains in endoglucanase B (CenB) from Cellulomonas fimi: functions and relatedness to domains in other polypeptides.
Journal of bacteriology 1991;173(22):7126-35.
1991: D Tull; Stephen G Withers; Neil Gilkes; Douglas G Kilburn; R Antony J Warren; Ruedi Aebersold
Glutamic acid 274 is the nucleophile in the active site of a "retaining" exoglucanase from Cellulomonas fimi.
The Journal of biological chemistry 1991;266(24):15621-5.
1991: H Shen; M Schmuck; I Pilz; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Deletion of the linker connecting the catalytic and cellulose-binding domains of endoglucanase A (CenA) of Cellulomonas fimi alters its conformation and catalytic activity.
The Journal of biological chemistry 1991;266(17):11335-40.
1991: Neil Gilkes; Bernard Henrissat; Douglas G Kilburn; R C Miller; R Antony J Warren
Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.
Microbiological reviews 1991;55(2):303-15.
1991: J B Coutinho; Bernhard Moser; Douglas G Kilburn; R Antony J Warren; R C Miller
Nucleotide sequence of the endoglucanase C gene (cenC) of Cellulomonas fimi, its high-level expression in Escherichia coli, and characterization of its products.
Molecular microbiology 1991;5(5):1221-33.
1991: A Meinke; C Braun; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Unusual sequence organization in CenB, an inverting endoglucanase from Cellulomonas fimi.
Journal of bacteriology 1991;173(1):308-14.
1991: E Ong; Neil Gilkes; R C Miller; A J Warren; Douglas G Kilburn
Enzyme immobilization using a cellulose-binding domain: properties of a beta-glucosidase fusion protein.
Enzyme and microbial technology 1991;13(1):59-65.
1991: Eric J Jervis; Douglas G Kilburn
Rapid immunoassay technique for process monitoring of animal cell fermentations.
Biotechnology progress 1991;7(1):28-32.
1990: I Pilz; E Schwarz; Douglas G Kilburn; R C Miller; R Antony J Warren; Neil Gilkes
The tertiary structure of a bacterial cellulase determined by small-angle X-ray-scattering analysis.
The Biochemical journal 1990;271(1):277-80.
1990: N Din; C F Beck; R C Miller; Douglas G Kilburn; R Antony J Warren
Expression of the Cellulomonas fimi cellulase genes cex and cenA from the divergent tet promoters of transposon Tn10.
Archives of microbiology 1990;153(2):129-33.
1989: Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Structural and functional analysis of a bacterial cellulase by proteolysis.
The Journal of biological chemistry 1989;264(30):17802-8.
1989: Bernhard Moser; Neil Gilkes; Douglas G Kilburn; R Antony J Warren; R C Miller
Purification and characterization of endoglucanase C of Cellulomonas fimi, cloning of the gene, and analysis of in vivo transcripts of the gene.
Applied and environmental microbiology 1989;55(10):2480-7.
1989: J M Greenwood; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Fusion to an endoglucanase allows alkaline phosphatase to bind to cellulose.
FEBS letters 1989;244(1):127-31.
1988: Neil Gilkes; R Antony J Warren; R C Miller; Douglas G Kilburn
Precise excision of the cellulose binding domains from two Cellulomonas fimi cellulases by a homologous protease and the effect on catalysis.
The Journal of biological chemistry 1988;263(21):10401-7.
1988: J B Owolabi; P Beguin; Douglas G Kilburn; R C Miller; R Antony J Warren
Expression in Escherichia coli of the Cellulomonas fimi Structural Gene for Endoglucanase B.
Applied and environmental microbiology 1988;54(2):518-23.
1988: Warren W Wakarchuk; N M Greenberg; Douglas G Kilburn; R C Miller; R Antony J Warren
Structure and transcription analysis of the gene encoding a cellobiase from Agrobacterium sp. strain ATCC 21400.
Journal of bacteriology 1988;170(1):301-7.
1987: M L Langsford; Neil Gilkes; Bhagirath Singh; Bernhard Moser; R C Miller; R Antony J Warren; Douglas G Kilburn
Glycosylation of bacterial cellulases prevents proteolytic cleavage between functional domains.
FEBS letters 1987;225(1-2):163-7.
1987: N M Greenberg; R Antony J Warren; Douglas G Kilburn; R C Miller
Regulation and initiation of cenB transcripts of Cellulomonas fimi.
Journal of bacteriology 1987;169(10):4674-7.
1987: F W Paradis; R Antony J Warren; Douglas G Kilburn; R C Miller
The expression of Cellulomonas fimi cellulase genes in Brevibacterium lactofermentum.
Gene 1987;61(2):199-206.
1987: R Antony J Warren; B Gerhard; Neil Gilkes; J B Owolabi; Douglas G Kilburn; R C Miller
A bifunctional exoglucanase-endoglucanase fusion protein.
Gene 1987;61(3):421-7.
1986: R Antony J Warren; C F Beck; Neil Gilkes; Douglas G Kilburn; M L Langsford; R C Miller; G P O'Neill; M Scheufens; W K Wong
Sequence conservation and region shuffling in an endoglucanase and an exoglucanase from Cellulomonas fimi.
Proteins 1986;1(4):335-41.
1986: W K Wong; B Gerhard; Z M Guo; Douglas G Kilburn; A J Warren; R C Miller
Characterization and structure of an endoglucanase gene cenA of Cellulomonas fimi.
Gene 1986;44(2-3):315-24.
1984: Neil Gilkes; M L Langsford; Douglas G Kilburn; R C Miller; R Antony J Warren
Mode of action and substrate specificities of cellulases from cloned bacterial genes.
The Journal of biological chemistry 1984;259(16):10455-9.