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Mitsuhiro Kinoshita

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Kinya Iwatsuka; Takao Hayakawa; Kazuya Kamisue; Mitsuhiro Kinoshita; Tadashi Suzuki; Sakie Watanabe; Keita Yamada; Kazuaki Kakehi
Free glycans derived from glycoproteins present in human sera.
Yosuke Mitsui; Keita Yamada; Sayaka Hara; Takao Hayakawa; Mitsuhiro Kinoshita; Kazuaki Kakehi
Comparative studies on glycoproteins expressing polylactosamine-type N-glycans in cancer cells.
Mitsuhiro Kinoshita; Naotaka Kakoi; Yu-Ki Matsuno; Takao Hayakawa; Kazuaki Kakehi
Determination of sulfate ester content in sulfated oligo- and poly-saccharides by capillary electrophoresis with indirect UV detection.

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All Publications

2013: Kinya Iwatsuka; Takao Hayakawa; Kazuya Kamisue; Mitsuhiro Kinoshita; Tadashi Suzuki; Sakie Watanabe; Keita Yamada; Kazuaki Kakehi
Free glycans derived from glycoproteins present in human sera.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2013;928():16-21.
2012: Yosuke Mitsui; Keita Yamada; Sayaka Hara; Takao Hayakawa; Mitsuhiro Kinoshita; Kazuaki Kakehi
Comparative studies on glycoproteins expressing polylactosamine-type N-glycans in cancer cells.
Journal of pharmaceutical and biomedical analysis 2012;70():718-26.
2011: Mitsuhiro Kinoshita; Naotaka Kakoi; Yu-Ki Matsuno; Takao Hayakawa; Kazuaki Kakehi
Determination of sulfate ester content in sulfated oligo- and poly-saccharides by capillary electrophoresis with indirect UV detection.
Biomedical chromatography : BMC 2011;25(5):588-93.
2010: Hirofumi Takada; Aiko Nishida; Mitsuhiro Domae; Ayako Kita; Yuki Yamano; Atsushi Uchida; Shunji Ishiwata; Yue Fang; Xin Zhou; Takashi Masuko; Mitsuhiro Kinoshita; Kazuaki Kakehi; Reiko Sugiura
The cell surface protein gene ecm33+ is a target of the two transcription factors Atf1 and Mbx1 and negatively regulates Pmk1 MAPK cell integrity signaling in fission yeast.
Molecular biology of the cell 2010;21(4):674-85.
2010: Keita Yamada; Sakie Watanabe; Soichiro Kita; Mitsuhiro Kinoshita; Takao Hayakawa; Kazuaki Kakehi
Determination of Tn antigen released from cultured cancer cells by capillary electrophoresis.
Analytical biochemistry 2010;396(1):161-3.
2009: Mitsuhiro Kinoshita; Hiroko Ohta; Kanata Higaki; Yoko Kojima; Tadasu Urashima; Kazuki Nakajima; Minoru Suzuki; Kit M Kovacs; Christian Lydersen; Takao Hayakawa; Kazuaki Kakehi
Structural characterization of multibranched oligosaccharides from seal milk by a combination of off-line high-performance liquid chromatography-matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry and sequential exoglycosidase digestion.
Analytical biochemistry 2009;388(2):242-53.
2009: Kazuaki Kakehi; Mitsuhiro Kinoshita
Capillary lectin-affinity electrophoresis for glycan analysis.
Methods in molecular biology (Clifton, N.J.) 2009;534():93-105.
2007: Keita Yamada; Satomi Hyodo; Yu-ki Matsuno; Mitsuhiro Kinoshita; Shu-zou Maruyama; Yu-suke Osaka; Enriqueta Casal; Yuan C Lee; Kazuaki Kakehi
Rapid and sensitive analysis of mucin-type glycans using an in-line flow glycan-releasing apparatus.
Analytical biochemistry 2007;371(1):52-61.
2007: Yu-Ki Matsuno; Keita Yamada; Ayumi Tanabe; Mitsuhiro Kinoshita; Shu-zou Maruyama; Yu-suke Osaka; Takashi Masuko; Kazuaki Kakehi
Development of an apparatus for rapid release of oligosaccharides at the glycosaminoglycan-protein linkage region in chondroitin sulfate-type proteoglycans.
Analytical biochemistry 2007;362(2):245-57.
2006: Satoru Kamoda; Yasuharu Nakanishi; Mitsuhiro Kinoshita; Rika Ishikawa; Kazuaki Kakehi
Analysis of glycoprotein-derived oligosaccharides in glycoproteins detected on two-dimensional gel by capillary electrophoresis using on-line concentration method.
Journal of chromatography. A 2006;1106(1-2):67-74.
2006: Ryosuke Naka; Satoru Kamoda; Aya Ishizuka; Mitsuhiro Kinoshita; Kazuaki Kakehi
Analysis of total N-glycans in cell membrane fractions of cancer cells using a combination of serotonin affinity chromatography and normal phase chromatography.
Journal of proteome research 2006;5(1):88-97.
2006: Kazuki Nakajima; Mitsuhiro Kinoshita; Namiko Matsushita; Tadasu Urashima; Minoru Suzuki; Akemi Suzuki; Kazuaki Kakehi
Capillary affinity electrophoresis using lectins for the analysis of milk oligosaccharide structure and its application to bovine colostrum oligosaccharides.
Analytical biochemistry 2006;348(1):105-14.
2005: Shin-ichi Yasueda; Kazuhiro Yamakawa; Yasuharu Nakanishi; Mitsuhiro Kinoshita; Kazuaki Kakehi
Decreased mucin concentrations in tear fluids of contact lens wearers.
Journal of pharmaceutical and biomedical analysis 2005;39(1-2):187-95.
2005: Yu-ki Matsuno; Mitsuhiro Kinoshita; Kazuaki Kakehi
Fast analysis of glycosaminoglycans by microchip electrophoresis with in situ fluorescent detection using ethidium bromide.
Journal of pharmaceutical and biomedical analysis 2005;37(3):429-36.
2005: Mitsuhiro Kinoshita; Kazuaki Kakehi
Analysis of the interaction between hyaluronan and hyaluronan-binding proteins by capillary affinity electrophoresis: significance of hyaluronan molecular size on binding reaction.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2005;816(1-2):289-95.
2004: Satoru Kamoda; Chie Nomura; Mitsuhiro Kinoshita; Saori Nishiura; Rika Ishikawa; Kazuaki Kakehi; Nana Kawasaki; Takao Hayakawa
Profiling analysis of oligosaccharides in antibody pharmaceuticals by capillary electrophoresis.
Journal of chromatography. A 2004;1050(2):211-6.
2004: Yu-Ki Matsuno; Mitsuhiro Kinoshita; Kazuaki Kakehi
Electrophoretic analysis of di- and oligosaccharides derived from glycosaminoglycans on microchip format.
Journal of pharmaceutical and biomedical analysis 2004;36(1):9-15.
2004: Kazuki Nakajima; Mitsuhiro Kinoshita; Yasuo Oda; Takashi Masuko; Hanae Kaku; Naoto Shibuya; Kazuaki Kakehi
Screening method of carbohydrate-binding proteins in biological sources by capillary affinity electrophoresis and its application to determination of Tulipa gesneriana agglutinin in tulip bulbs.
Glycobiology 2004;14(9):793-804.
2004: Tomonori Hayashi; Shin-ichi Yasueda; Yasuharu Nakanishi; Hiroko Ohta; Mitsuhiro Kinoshita; Yasuyoshi Miki; Takashi Masuko; Kazuaki Kakehi
Capturing of acidic macromolecules from biological samples using a temperature-responsive polymer modified with poly-l-lysine.
The Analyst 2004;129(5):421-7.
2004: Atsufumi Kawabata; Hiroyuki Nishikawa; Hitomi Saitoh; Yumiko Nakaya; Kaori Hiramatsu; Satoko Kubo; Minoru Nishida; Naoyuki Kawao; Ryotaro Kuroda; Fumiko Sekiguchi; Mitsuhiro Kinoshita; Kazuaki Kakehi; Naoki Arizono; Hisakazu Yamagishi; Kenzo Kawai
A protective role of protease-activated receptor 1 in rat gastric mucosa.
Gastroenterology 2004;126(1):208-19.
2003: Kazuaki Kakehi; Mitsuhiro Kinoshita; Shin-ichi Yasueda
Hyaluronic acid: separation and biological implications.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2003;797(1-2):347-55.
2003: Yasuo Oda; Tsutomu Senaha; Yuuki Matsuno; Kazuki Nakajima; Ryousuke Naka; Mitsuhiro Kinoshita; Eiko Honda; Itaru Furuta; Kazuaki Kakehi
A new fungal lectin recognizing alpha(1-6)-linked fucose in the N-glycan.
The Journal of biological chemistry 2003;278(34):32439-47.
2003: Ryoko Akai; Mitsuhiro Kinoshita; Kazuaki Kakehi; Yuan C Lee
A method for detecting O-glycanase in biological samples using a combination of MALDI-TOF mass spectrometry and time-resolved fluorimetry.
The Analyst 2003;128(5):440-6.
2003: Kazuaki Kakehi; Mitsuhiro Kinoshita; Yasuo Oda; Badarulhisam Abdul-Rahman
Lectin from bulbs of Crocus sativus recognizing N-linked core glycan: isolation and binding studies using fluorescence polarization.
Methods in enzymology 2003;362():512-22.
2003: Kazuki Nakajima; Yasuo Oda; Mitsuhiro Kinoshita; Kazuaki Kakehi
Capillary affinity electrophoresis for the screening of post-translational modification of proteins with carbohydrates.
Journal of proteome research 2003;2(1):81-8.
2002: Kazuki Nakajima; Yasuo Oda; Mitsuhiro Kinoshita; Takashi Masuko; Kazuaki Kakehi
Time-resolved fluorometric analysis of carbohydrates labeled with amino-aromatic compounds by reductive amination.
The Analyst 2002;127(7):972-6.
2002: Kazuo Sei; Miyako Nakano; Mitsuhiro Kinoshita; Takashi Masuko; Kazuaki Kakehi
Collection of alpha1-acid glycoprotein molecular species by capillary electrophoresis and the analysis of their molecular masses and carbohydrate chains. Basic studies on the analysis of glycoprotein glycoforms.
Journal of chromatography. A 2002;958(1-2):273-81.
2002: Atsufumi Kawabata; Mitsuhiro Kinoshita; Ryotaro Kuroda; Kazuaki Kakehi
Capsazepine partially inhibits neurally mediated gastric mucus secretion following activation of protease-activated receptor 2.
Clinical and experimental pharmacology & physiology 2002;29(4):360-1.
2002: Mitsuhiro Kinoshita; Hiroyuki Shiraishi; Chiho Muranushi; Naomichi Mitsumori; Takao Ando; Yasuo Oda; Kazuaki Kakehi
Determination of molecular mass of acidic polysaccharides by capillary electrophoresis.
Biomedical chromatography : BMC 2002;16(2):141-5.
2002: Kazuaki Kakehi; Mitsuhiro Kinoshita; Miyako Nakano
Analysis of glycoproteins and the oligosaccharides thereof by high-performance capillary electrophoresis-significance in regulatory studies on biopharmaceutical products.
Biomedical chromatography : BMC 2002;16(2):103-15.
2001: N Morimoto; M Nakano; Mitsuhiro Kinoshita; Atsufumi Kawabata; M Morita; Yasuo Oda; Ryotaro Kuroda; Kazuaki Kakehi
Specific distribution of sialic acids in animal tissues as examined by LC-ESI-MS after derivatization with 1,2-diamino-4,5-methylenedioxybenzene.
Analytical chemistry 2001;73(22):5422-8.
2001: Kazuaki Kakehi; Yasuo Oda; Mitsuhiro Kinoshita
Fluorescence polarization: analysis of carbohydrate-protein interaction.
Analytical biochemistry 2001;297(2):111-6.
2001: Kazuaki Kakehi; Mitsuhiro Kinoshita; D Kawakami; J Tanaka; K Sei; K Endo; Yasuo Oda; M Iwaki; Takashi Masuko
Capillary electrophoresis of sialic acid-containing glycoprotein. Effect of the heterogeneity of carbohydrate chains on glycoform separation using an alpha1-acid glycoprotein as a model.
Analytical chemistry 2001;73(11):2640-7.
2001: Atsufumi Kawabata; Mitsuhiro Kinoshita; Hiroyuki Nishikawa; Ryotaro Kuroda; Minoru Nishida; H Araki; Naoki Arizono; Yasuo Oda; Kazuaki Kakehi
The protease-activated receptor-2 agonist induces gastric mucus secretion and mucosal cytoprotection.
The Journal of clinical investigation 2001;107(11):1443-50.
2000: Yasuo Oda; K Nakayama; Badarulhisam Abdul-Rahman; Mitsuhiro Kinoshita; O Hashimoto; N Kawasaki; T Hayakawa; Kazuaki Kakehi; Noboru Tomiya; Yuan C Lee
Crocus sativus lectin recognizes Man3GlcNAc in the N-glycan core structure.
The Journal of biological chemistry 2000;275(35):26772-9.
2000: Mitsuhiro Kinoshita; K Inagake; Atsufumi Kawabata; Ryotaro Kuroda; Yasuo Oda; Kazuaki Kakehi
Fluorometric determination of mucin-type glycoproteins by the galactose oxidase-peroxidase method.
Analytical biochemistry 2000;284(1):87-92.
2000: Atsufumi Kawabata; N Morimoto; Yasuo Oda; Mitsuhiro Kinoshita; Ryotaro Kuroda; Kazuaki Kakehi
Determination of mucin in salivary glands using sialic acids as the marker by high-performance liquid chromatography with fluorometric detection.
Analytical biochemistry 2000;283(1):119-21.
2000: Mitsuhiro Kinoshita; E Murakami; Yasuo Oda; T Funakubo; D Kawakami; Kazuaki Kakehi; N Kawasaki; K Morimoto; T Hayakawa
Comparative studies on the analysis of glycosylation heterogeneity of sialic acid-containing glycoproteins using capillary electrophoresis.
Journal of chromatography. A 2000;866(2):261-71.
1999: Yasuo Oda; Mitsuhiro Kinoshita; K Nakayama; S Ikeda; Kazuaki Kakehi
Flow injection analysis of binding reaction between fluorescent lectin and cells.
Analytical biochemistry 1999;269(2):230-5.
1999: Kazuaki Kakehi; Mitsuhiro Kinoshita; S Hayase; Yasuo Oda
Capillary electrophoresis of N-acetylneuraminic acid polymers and hyaluronic acid: correlation between migration order reversal and biological functions.
Analytical chemistry 1999;71(8):1592-6.
1998: Yasuo Oda; Mitsuhiro Kinoshita; K Nakayama; Kazuaki Kakehi
Evaluation of fluorescence polarization method for binding study in carbohydrate-lectin interaction.
Biological & pharmaceutical bulletin 1998;21(11):1215-7.
1997: Yasuo Oda; Mitsuhiro Kinoshita; Kazuaki Kakehi
Fluorometric assay of binding specificity of plant lectins to yeast cells by biotin-avidin system and its application to the classification of yeast cells.
Analytical biochemistry 1997;254(1):41-8.