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Dieter Kluepfel

Research Profile

Living Beings

Streptomyces

Concepts & Ideas

Chemicals & Drugs

Genes & Molecular Sequences

Procedures

Cloning, Molecular

Physiology

Mutagenesis, Site-Directed

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Publications

TOP 3
Martin Roberge; Ruthven N A H Lewis; François Shareck; Rolf Morosoli; Dieter Kluepfel; Claude Dupont; Ronald N McElhaney
Differential scanning calorimetric, circular dichroism, and Fourier transform infrared spectroscopic characterization of the thermal unfolding of xylanase A from Streptomyces lividans.
Valérie M-A Ducros; Simon Charnock; Urszula Derewenda; Zygmunt S Derewenda; Zbigniew Dauter; Claude Dupont; François Shareck; Rolf Morosoli; Dieter Kluepfel; Gideon J Davies
Substrate specificity in glycoside hydrolase family 10. Structural and kinetic analysis of the Streptomyces lividans xylanase 10A.
Martin Roberge; François Shareck; Rolf Morosoli; Dieter Kluepfel; Claude Dupont
Characterization of active-site aromatic residues in xylanase A from Streptomyces lividans.

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All Publications

2003: Martin Roberge; Ruthven N A H Lewis; François Shareck; Rolf Morosoli; Dieter Kluepfel; Claude Dupont; Ronald N McElhaney
Differential scanning calorimetric, circular dichroism, and Fourier transform infrared spectroscopic characterization of the thermal unfolding of xylanase A from Streptomyces lividans.
Proteins 2003;50(2):341-54.
2000: Valérie M-A Ducros; Simon Charnock; Urszula Derewenda; Zygmunt S Derewenda; Zbigniew Dauter; Claude Dupont; François Shareck; Rolf Morosoli; Dieter Kluepfel; Gideon J Davies
Substrate specificity in glycoside hydrolase family 10. Structural and kinetic analysis of the Streptomyces lividans xylanase 10A.
The Journal of biological chemistry 2000;275(30):23020-6.
1999: Martin Roberge; François Shareck; Rolf Morosoli; Dieter Kluepfel; Claude Dupont
Characterization of active-site aromatic residues in xylanase A from Streptomyces lividans.
Protein engineering 1999;12(3):251-7.
1998: Martin Roberge; François Shareck; Rolf Morosoli; Dieter Kluepfel; Claude Dupont
Site-directed mutagenesis study of a conserved residue in family 10 glycanases: histidine 86 of xylanase A from Streptomyces lividans.
Protein engineering 1998;11(5):399-404.
1998: Claude Dupont; Martin Roberge; François Shareck; Rolf Morosoli; Dieter Kluepfel
Substrate-binding domains of glycanases from Streptomyces lividans: characterization of a new family of xylan-binding domains.
The Biochemical journal 1998;330 ( Pt 1)():41-5.
1997: Martin Roberge; François Shareck; Rolf Morosoli; Dieter Kluepfel; Claude Dupont
Characterization of two important histidine residues in the active site of xylanase A from Streptomyces lividans, a family 10 glycanase.
Biochemistry 1997;36(25):7769-75.
1997: Martin Roberge; Claude Dupont; Rolf Morosoli; François Shareck; Dieter Kluepfel
Asparagine-127 of xylanase A from Streptomyces lividans, a key residue in glycosyl hydrolases of superfamily 4/7: kinetic evidence for its involvement in stabilization of the catalytic intermediate.
Protein engineering 1997;10(4):399-403.
1997: P Vincent; François Shareck; Claude Dupont; Rolf Morosoli; Dieter Kluepfel
New alpha-L-arabinofuranosidase produced by Streptomyces lividans: cloning and DNA sequence of the abfB gene and characterization of the enzyme.
The Biochemical journal 1997;322 ( Pt 3)():845-52.
1997: Rolf Morosoli; François Shareck; Dieter Kluepfel
Protein secretion in streptomycetes.
FEMS microbiology letters 1997;146(2):167-74.
1996: Peter Biely; G L Côté; L Kremnický; R V Greene; C Dupont; Dieter Kluepfel
Substrate specificity and mode of action of acetylxylan esterase from Streptomyces lividans.
FEBS letters 1996;396(2-3):257-60.
1996: Claude Dupont; N Daigneault; François Shareck; Rolf Morosoli; Dieter Kluepfel
Purification and characterization of an acetyl xylan esterase produced by Streptomyces lividans.
The Biochemical journal 1996;319 ( Pt 3)():881-6.
1996: S Ostiguy; M Gilbert; François Shareck; Dieter Kluepfel; Rolf Morosoli
Cloning and sequencing of the secY homolog from Streptomyces lividans 1326.
Gene 1996;176(1-2):265-7.
1996: M Gilbert; S Ostiguy; Dieter Kluepfel; Rolf Morosoli
Cloning of a secA homolog from Streptomyces lividans 1326 and overexpression in both S. lividans and Escherichia coli.
Biochimica et biophysica acta 1996;1296(1):9-12.
1996: N Pagé; Dieter Kluepfel; François Shareck; Rolf Morosoli
Increased xylanase yield in Streptomyces lividans: dependence on number of ribosome-binding sites.
Nature biotechnology 1996;14(6):756-9.
1996: F W Paradis; François Shareck; Claude Dupont; Dieter Kluepfel; Rolf Morosoli
Expression and secretion of beta-glucuronidase and Pertussis toxin S1 by Streptomyces lividans.
Applied microbiology and biotechnology 1996;45(5):646-51.
1996: Claude Dupont; Dieter Kluepfel; Rolf Morosoli
Evidence for lysozyme-type mechanism of hydrolysis in xylanases.
EXS 1996;75():411-23.
1996: N Pagé; Dieter Kluepfel; François Shareck; Rolf Morosoli
Effect of signal peptide alterations and replacement on export of xylanase A in Streptomyces lividans.
Applied and environmental microbiology 1996;62(1):109-14.
1995: Y Hurtubise; François Shareck; Dieter Kluepfel; Rolf Morosoli
A cellulase/xylanase-negative mutant of Streptomyces lividans 1326 defective in cellobiose and xylobiose uptake is mutated in a gene encoding a protein homologous to ATP-binding proteins.
Molecular microbiology 1995;17(2):367-77.
1995: François Shareck; Peter Biely; Rolf Morosoli; Dieter Kluepfel
Analysis of DNA flanking the xlnB locus of Streptomyces lividans reveals genes encoding acetyl xylan esterase and the RNA component of ribonuclease P.
Gene 1995;153(1):105-9.
1995: M Gilbert; Rolf Morosoli; François Shareck; Dieter Kluepfel
Production and secretion of proteins by streptomycetes.
Critical reviews in biotechnology 1995;15(1):13-39.
1994: C Manin; F Shareek; Rolf Morosoli; Dieter Kluepfel
Purification and characterization of an alpha-L-arabinofuranosidase from Streptomyces lividans 66 and DNA sequence of the gene (abfA).
The Biochemical journal 1994;302 ( Pt 2)():443-9.
1994: Urszula Derewenda; L Swenson; R Green; Y Wei; Rolf Morosoli; François Shareck; Dieter Kluepfel; Zygmunt S Derewenda
Crystal structure, at 2.6-A resolution, of the Streptomyces lividans xylanase A, a member of the F family of beta-1,4-D-glycanases.
The Journal of biological chemistry 1994;269(33):20811-4.
1994: A Moreau; Martin Roberge; C Manin; François Shareck; Dieter Kluepfel; Rolf Morosoli
Identification of two acidic residues involved in the catalysis of xylanase A from Streptomyces lividans.
The Biochemical journal 1994;302 ( Pt 1)():291-5.
1994: F F Arhin; François Shareck; Dieter Kluepfel; Rolf Morosoli
Effects of disruption of xylanase-encoding genes on the xylanolytic system of Streptomyces lividans.
Journal of bacteriology 1994;176(16):4924-30.
1994: S Wittmann; François Shareck; Dieter Kluepfel; Rolf Morosoli
Purification and characterization of the CelB endoglucanase from Streptomyces lividans 66 and DNA sequence of the encoding gene.
Applied and environmental microbiology 1994;60(5):1701-3.
1994: A Moreau; François Shareck; Dieter Kluepfel; Rolf Morosoli
Increase in catalytic activity and thermostability of the xylanase A of Streptomyces lividans 1326 by site-specific mutagenesis.
Enzyme and microbial technology 1994;16(5):420-4.
1994: A Moreau; François Shareck; Dieter Kluepfel; Rolf Morosoli
Alteration of the cleavage mode and of the transglycosylation reactions of the xylanase A of Streptomyces lividans 1326 by site-directed mutagenesis of the Asn173 residue.
European journal of biochemistry / FEBS 1994;219(1-2):261-6.
1993: Peter Biely; Dieter Kluepfel; Rolf Morosoli; François Shareck
Mode of action of three endo-beta-1,4-xylanases of Streptomyces lividans.
Biochimica et biophysica acta 1993;1162(3):246-54.
1993: N Arcand; Dieter Kluepfel; F W Paradis; Rolf Morosoli; François Shareck
Beta-mannanase of Streptomyces lividans 66: cloning and DNA sequence of the manA gene and characterization of the enzyme.
The Biochemical journal 1993;290 ( Pt 3)():857-63.
1992: A Moreau; F W Paradis; Rolf Morosoli; François Shareck; Dieter Kluepfel
A novel shuttle vector for Streptomyces spp. and Escherichia coli as a tool in site-directed mutagenesis.
Canadian journal of microbiology 1992;38(4):350-3.
1992: M Théberge; P Lacaze; François Shareck; Rolf Morosoli; Dieter Kluepfel
Purification and characterization of an endoglucanase from Streptomyces lividans 66 and DNA sequence of the gene.
Applied and environmental microbiology 1992;58(3):815-20.
1991: François Shareck; C Roy; M Yaguchi; Rolf Morosoli; Dieter Kluepfel
Sequences of three genes specifying xylanases in Streptomyces lividans.
Gene 1991;107(1):75-82.
1990: Dieter Kluepfel; S Vats-Mehta; F Aumont; François Shareck; Rolf Morosoli
Purification and characterization of a new xylanase (xylanase B) produced by Streptomyces lividans 66.
The Biochemical journal 1990;267(1):45-50.
1990: S Vats-Mehta; P Bouvrette; François Shareck; Rolf Morosoli; Dieter Kluepfel
Cloning of a second xylanase-encoding gene of Streptomyces lividans 66.
Gene 1990;86(1):119-22.
1986: Rolf Morosoli; J L Bertrand; F Mondou; François Shareck; Dieter Kluepfel
Purification and properties of a xylanase from Streptomyces lividans.
The Biochemical journal 1986;239(3):587-92.
1986: F Mondou; François Shareck; Rolf Morosoli; Dieter Kluepfel
Cloning of the xylanase gene of Streptomyces lividans.
Gene 1986;49(3):323-9.
1983: N Moldoveanu; Dieter Kluepfel
Comparison of beta-Glucosidase Activities in Different Streptomyces Strains.
Applied and environmental microbiology 1983;46(1):17-21.