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Renate Kunert

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TOP 3
Alexander Mader; Bernhard Prewein; Katalin Zboray; Emilio Casanova; Renate Kunert
Exploration of BAC versus plasmid expression vectors in recombinant CHO cells.
Vaibhav Jadhav; Renate Kunert; Matthias Wieser; Juan A Hernandez Bort; Nicole Borth; Matthias Hackl; Eva Harreither; Johannes Grillari
A screening method to assess biological effects of microRNA overexpression in Chinese hamster ovary cells.
David Reinhart; Robert Weik; Renate Kunert
Recombinant IgA production: single step affinity purification using camelid ligands and product characterization.

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All Publications

2013: Alexander Mader; Bernhard Prewein; Katalin Zboray; Emilio Casanova; Renate Kunert
Exploration of BAC versus plasmid expression vectors in recombinant CHO cells.
Applied microbiology and biotechnology 2013;97(9):4049-54.
2012: Vaibhav Jadhav; Renate Kunert; Matthias Wieser; Juan A Hernandez Bort; Nicole Borth; Matthias Hackl; Eva Harreither; Johannes Grillari
A screening method to assess biological effects of microRNA overexpression in Chinese hamster ovary cells.
Biotechnology and bioengineering 2012;109(6):1376-85.
2012: David Reinhart; Robert Weik; Renate Kunert
Recombinant IgA production: single step affinity purification using camelid ligands and product characterization.
Journal of immunological methods 2012;378(1-2):95-101.
2012: Michael Taschwer; Friedrich Altmann; Josephine Grass; Matthias Hackl; Juan A Hernández Bort; Niraj Kumar; Renate Kunert; Christian Leitner; Martin Papst; Urszula Puc; Nicole Borth
Growth, productivity and protein glycosylation in a CHO EpoFc producer cell line adapted to glutamine-free growth.
Journal of biotechnology 2012;157(2):295-303.
2012: Nerea Huarte; Renate Kunert; Maier Lorizate; Aitziber Araujo; Rocio Arranz; Heribert Quendler; José M Valpuesta; José L Nieva
Recognition of membrane-bound fusion-peptide/MPER complexes by the HIV-1 neutralizing 2F5 antibody: implications for anti-2F5 immunogenicity.
PloS one 2012;7(12):e52740.
2012: Alexander Mader; Renate Kunert
Evaluation of the potency of the anti-idiotypic antibody Ab2/3H6 mimicking gp41 as an HIV-1 vaccine in a rabbit prime/boost study.
PloS one 2012;7(6):e39063.
2012: Renate Kunert; Karola Vorauer-Uhl
Strategies for efficient transfection of CHO-cells with plasmid DNA.
Methods in molecular biology (Clifton, N.J.) 2012;801():213-26.
2011: Matthias Hackl; Tobias Jakobi; Jochen Blom; Daniel Doppmeier; Karina Brinkrolf; Rafael Szczepanowski; Stephan H Bernhart; Juan Antonio Hernandez Bort; Matthias Wieser; Renate Kunert; Simon Jeffs; Ivo L Hofacker; Alexander Goesmann; Alfred Pühler; Nicole Borth; Christian Höner Zu Siederdissen; Johannes Grillari
Next-generation sequencing of the Chinese hamster ovary microRNA transcriptome: Identification, annotation and profiling of microRNAs as targets for cellular engineering.
Journal of biotechnology 2011;153(1-2):62-75.
2011: Andreas Loos; Bart Van Droogenbroeck; Stefan Hillmer; Josephine Grass; Martin Pabst; Alexandra Castilho; Renate Kunert; Mifang Liang; Elsa Arcalis; David G Robinson; Ann Depicker; Herta Steinkellner
Expression of antibody fragments with a controlled N-glycosylation pattern and induction of endoplasmic reticulum-derived vesicles in seeds of Arabidopsis.
Plant physiology 2011;155(4):2036-48.
2011: Andreas Loos; Bart Van Droogenbroeck; Stefan Hillmer; Josephine Grass; Renate Kunert; Jingyuan Cao; David G Robinson; Ann Depicker; Herta Steinkellner
Production of monoclonal antibodies with a controlled N-glycosylation pattern in seeds of Arabidopsis thaliana.
Plant biotechnology journal 2011;9(2):179-92.
2010: A Mader; Renate Kunert
Humanization strategies for an anti-idiotypic antibody mimicking HIV-1 gp41.
Protein engineering, design & selection : PEDS 2010;23(12):947-54.
2010: Karola Vorauer-Uhl; Jakob Wallner; Gabriele Lhota; Hermann Katinger; Renate Kunert
IgM characterization directly performed in crude culture supernatants by a new simple electrophoretic method.
Journal of immunological methods 2010;359(1-2):21-7.
2010: Helga Böhm-Hofstätter; Marion Tschernutter; Renate Kunert
Comparison of hybridization methods and real-time PCR: their value in animal cell line characterization.
Applied microbiology and biotechnology 2010;87(2):419-25.
2010: Alexandra Castilho; Richard Strasser; Johannes Stadlmann; Josephine Grass; Jakub Jez; Pia Gattinger; Renate Kunert; Heribert Quendler; Martin Pabst; Renaud Leonard; Friedrich Altmann; Herta Steinkellner
In planta protein sialylation through overexpression of the respective mammalian pathway.
The Journal of biological chemistry 2010;285(21):15923-30.
2010: Stefanie Strobach; Renate Kunert; Johannes Stadlmann; Paul Messner; Eva Sevcsik; Gabriele Lhota; Hermann Katinger; Karola Vorauer-Uhl
Topological transformation of liposomes by a membrane-affecting domain of recombinant human erythropoietin.
Journal of liposome research 2010;20(1):24-30.
2010: Johannes Gach; Paul Georg Furtmüller; Heribert Quendler; Paul Messner; Ralf Wagner; Hermann Katinger; Renate Kunert
Proline is not uniquely capable of providing the pivot point for domain swapping in 2G12, a broadly neutralizing antibody against HIV-1.
The Journal of biological chemistry 2010;285(2):1122-7.
2009: Johannes Stadlmann; Alfred Weber; Martin Pabst; Heinz Anderle; Renate Kunert; Hartmut J Ehrlich; Hans Peter Schwarz; Friedrich Altmann
A close look at human IgG sialylation and subclass distribution after lectin fractionation.
Proteomics 2009;9(17):4143-53.
2009: Renate Kunert; Willibald Steinfellner; Friedrich Altmann; Jakob Wallner; Hermann Katinger; Karola Vorauer-Uhl
CHO-recombinant human growth hormone as a protease sensitive reporter protein.
Applied microbiology and biotechnology 2009;84(4):693-9.
2009: Hannes Reisinger; Willibald Steinfellner; Hermann Katinger; Renate Kunert
Serum-free transfection of CHO cells with chemically defined transfection systems and investigation of their potential for transient and stable transfection.
Cytotechnology 2009;60(1-3):115-23.
2008: Hannes Reisinger; Willibald Steinfellner; Beate Stern; Hermann Katinger; Renate Kunert
The absence of effect of gene copy number and mRNA level on the amount of mAb secretion from mammalian cells.
Applied microbiology and biotechnology 2008;81(4):701-10.
2008: Johannes Stadlmann; Martin Pabst; Daniel Kolarich; Renate Kunert; Friedrich Altmann
Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides.
Proteomics 2008;8(14):2858-71.
2008: Evelyn Trummer; Wolfgang Ernst; Friedemann Hesse; Kornelia Schriebl; Christine Lattenmayer; Renate Kunert; Karola Vorauer-Uhl; Hermann Katinger; Dethardt Müller
Transcriptional profiling of phenotypically different Epo-Fc expressing CHO clones by cross-species microarray analysis.
Biotechnology journal 2008;3(7):924-37.
2008: Johannes Gach; Heribert Quendler; Boris Ferko; Hermann Katinger; Renate Kunert
Expression, purification, and in vivo administration of a promising anti-idiotypic HIV-1 vaccine.
Molecular biotechnology 2008;39(2):119-25.
2008: Thomas Rademacher; Markus Sack; Elsa Arcalis; Johannes Stadlmann; Simone Balzer; Friedrich Altmann; Heribert Quendler; Gabriela Stiegler; Renate Kunert; Rainer Fischer; Eva Stoger
Recombinant antibody 2G12 produced in maize endosperm efficiently neutralizes HIV-1 and contains predominantly single-GlcNAc N-glycans.
Plant biotechnology journal 2008;6(2):189-201.
2008: Johannes Gach; Heribert Quendler; Stefanie Strobach; Hermann Katinger; Renate Kunert
Structural analysis and in vivo administration of an anti-idiotypic antibody against mAb 2F5.
Molecular immunology 2008;45(4):1027-34.
2007: Johannes Gach; Heribert Quendler; Robert Weik; Hermann Katinger; Renate Kunert
Partial humanization and characterization of an anti-idiotypic antibody against monoclonal antibody 2F5, a potential HIV vaccine?
AIDS research and human retroviruses 2007;23(11):1405-15.
2007: Brigitta Vcelar; Gabriela Stiegler; Hermann M Wolf; Wolfgang Muntean; Bettina Leschnik; Saurabh Mehandru; Martin Markowitz; Christine Armbruster; Renate Kunert; Martha M Eibl; Hermann Katinger
Reassessment of autoreactivity of the broadly neutralizing HIV antibodies 4E10 and 2F5 and retrospective analysis of clinical safety data.
AIDS (London, England) 2007;21(16):2161-70.
2007: Matthias Schähs; Richard Strasser; Johannes Stadlmann; Renate Kunert; Thomas Rademacher; Herta Steinkellner
Production of a monoclonal antibody in plants with a humanized N-glycosylation pattern.
Plant biotechnology journal 2007;5(5):657-63.
2007: Hannes Reisinger; Eva Sevcsik; Karola Vorauer-Uhl; Karl Lohner; Hermann Katinger; Renate Kunert
Serum-free transfection of CHO-cells with tailor-made unilamellar vesicles.
Cytotechnology 2007;54(3):157-68.
2007: Kornelia Schriebl; Evelyn Trummer; Robert Weik; Christine Lattenmayer; Dethardt Müller; Renate Kunert; Hermann Katinger; Karola Vorauer-Uhl
Applicability of different fluorescent dyes for isoform quantification on linear IPG gels.
Electrophoresis 2007;28(12):2100-7.
2007: Markus Sack; Antje Paetz; Renate Kunert; Michael Bomble; Friedemann Hesse; Gabriela Stiegler; Rainer Fischer; Hermann Katinger; Eva Stoeger; Thomas Rademacher
Functional analysis of the broadly neutralizing human anti-HIV-1 antibody 2F5 produced in transgenic BY-2 suspension cultures.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007;21(8):1655-64.
2007: Georg Hinterkörner; Gudrun Brugger; Dethardt Müller; Friedemann Hesse; Renate Kunert; Hermann Katinger; Nicole Borth
Improvement of the energy metabolism of recombinant CHO cells by cell sorting for reduced mitochondrial membrane potential.
Journal of biotechnology 2007;129(4):651-7.
2007: Christine Lattenmayer; Martina Loeschel; Kornelia Schriebl; Willibald Steinfellner; Thomas Sterovsky; Evelyn Trummer; Karola Vorauer-Uhl; Dethardt Müller; Hermann Katinger; Renate Kunert
Protein-free transfection of CHO host cells with an IgG-fusion protein: selection and characterization of stable high producers and comparison to conventionally transfected clones.
Biotechnology and bioengineering 2007;96(6):1118-26.
2007: Christine Lattenmayer; Evelyn Trummer; Kornelia Schriebl; Karola Vorauer-Uhl; Dethardt Mueller; Hermann Katinger; Renate Kunert
Characterisation of recombinant CHO cell lines by investigation of protein productivities and genetic parameters.
Journal of biotechnology 2007;128(4):716-25.
2007: Johannes Gach; Michael Maurer; Rainer Hahn; Brigitte Gasser; Diethard Mattanovich; Hermann Katinger; Renate Kunert
High level expression of a promising anti-idiotypic antibody fragment vaccine against HIV-1 in Pichia pastoris.
Journal of biotechnology 2007;128(4):735-46.
2006: Kornelia Schriebl; Evelyn Trummer; Christine Lattenmayer; Robert Weik; Renate Kunert; Dethardt Müller; Hermann Katinger; Karola Vorauer-Uhl
Biochemical characterization of rhEpo-Fc fusion protein expressed in CHO cells.
Protein expression and purification 2006;49(2):265-75.
2006: Kornelia Schriebl; Evelyn Trummer; Robert Weik; Dethardt Müller; Renate Kunert; Christine Lattenmayer; Hermann Katinger; Karola Vorauer-Uhl
A novel strategy for quantitative isoform detection directly performed from culture supernatant.
Journal of pharmaceutical and biomedical analysis 2006;42(3):322-7.
2006: Evelyn Trummer; Katharina Fauland; Silke Seidinger; Kornelia Schriebl; Christine Lattenmayer; Renate Kunert; Karola Vorauer-Uhl; Robert Weik; Nicole Borth; Hermann Katinger; Dethardt Müller
Process parameter shifting: Part II. Biphasic cultivation-A tool for enhancing the volumetric productivity of batch processes using Epo-Fc expressing CHO cells.
Biotechnology and bioengineering 2006;94(6):1045-52.
2006: Evelyn Trummer; Katharina Fauland; Silke Seidinger; Kornelia Schriebl; Christine Lattenmayer; Renate Kunert; Karola Vorauer-Uhl; Robert Weik; Nicole Borth; Hermann Katinger; Dethardt Müller
Process parameter shifting: Part I. Effect of DOT, pH, and temperature on the performance of Epo-Fc expressing CHO cells cultivated in controlled batch bioreactors.
Biotechnology and bioengineering 2006;94(6):1033-44.
2006: Christine Lattenmayer; Martina Loeschel; Willibald Steinfellner; Evelyn Trummer; Dethardt Mueller; Kornelia Schriebl; Karola Vorauer-Uhl; Hermann Katinger; Renate Kunert
Identification of transgene integration loci of different highly expressing recombinant CHO cell lines by FISH.
Cytotechnology 2006;51(3):171-82.
2006: Brigitte Gasser; Michael Maurer; Johannes Gach; Renate Kunert; Diethard Mattanovich
Engineering of Pichia pastoris for improved production of antibody fragments.
Biotechnology and bioengineering 2006;94(2):353-61.
2006: Renate Kunert; Johannes Gach; Karola Vorauer-Uhl; Edwin Engel; Hermann Katinger
Validated method for quantification of genetically modified organisms in samples of maize flour.
Journal of agricultural and food chemistry 2006;54(3):678-81.
2005: Ernst Böhm; Johannes Grillari; Regina Voglauer; Stefan Gross; Wolfgang Ernst; Boris Ferko; Renate Kunert; Hermann Katinger; Nicole Borth
Establishment of a strategy for the rapid generation of a monoclonal antibody against the human protein SNEV (hNMP200) by flow-cytometric cell sorting.
Journal of immunological methods 2005;307(1-2):13-23.
2005: Sabine Nakowitsch; Heribert Quendler; Helga Fekete; Renate Kunert; Hermann Katinger; Gabriela Stiegler
HIV-1 mutants escaping neutralization by the human antibodies 2F5, 2G12, and 4E10: in vitro experiments versus clinical studies.
AIDS (London, England) 2005;19(17):1957-66.
2005: Barton F Haynes; Judith Fleming; E William St Clair; Hermann Katinger; Gabriela Stiegler; Renate Kunert; James Robinson; Richard M Scearce; Kelly Plonk; Herman F Staats; Thomas L Ortel; Hua-Xin Liao; S Munir Alam
Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodies.
Science (New York, N.Y.) 2005;308(5730):1906-8.
2005: Rosa M F Cardoso; Michael B Zwick; Robyn L Stanfield; Renate Kunert; James M Binley; Hermann Katinger; Dennis R Burton; Ian A Wilson
Broadly neutralizing anti-HIV antibody 4E10 recognizes a helical conformation of a highly conserved fusion-associated motif in gp41.
Immunity 2005;22(2):163-73.
2005: Nicole Borth; Diethard Mattanovich; Renate Kunert; Hermann Katinger
Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell line.
Biotechnology progress 2005;21(1):106-11.
2005: Michael B Zwick; Richard Jensen; Sarah Church; Meng Wang; Gabriela Stiegler; Renate Kunert; Hermann Katinger; Dennis R Burton
Anti-human immunodeficiency virus type 1 (HIV-1) antibodies 2F5 and 4E10 require surprisingly few crucial residues in the membrane-proximal external region of glycoprotein gp41 to neutralize HIV-1.
Journal of virology 2005;79(2):1252-61.
2004: Ernst Böhm; Regina Voglauer; Willibald Steinfellner; Renate Kunert; Nicole Borth; Hermann Katinger
Screening for improved cell performance: selection of subclones with altered production kinetics or improved stability by cell sorting.
Biotechnology and bioengineering 2004;88(6):699-706.
2004: James M Binley; Terri Wrin; Bette Korber; Michael B Zwick; Meng Wang; Colombe Chappey; Gabriela Stiegler; Renate Kunert; Susan Zolla-Pazner; Hermann Katinger; Christos J Petropoulos; Dennis R Burton
Comprehensive cross-clade neutralization analysis of a panel of anti-human immunodeficiency virus type 1 monoclonal antibodies.
Journal of virology 2004;78(23):13232-52.
2004: Kenneth H Roux; Ping Zhu; Margaret Seavy; Hermann Katinger; Renate Kunert; Vanessa Seamon
Electron microscopic and immunochemical analysis of the broadly neutralizing HIV-1-specific, anti-carbohydrate antibody, 2G12.
Molecular immunology 2004;41(10):1001-11.
2004: Renate Kunert; Susanne Wolbank; Gabriela Stiegler; Robert Weik; Hermann Katinger
Characterization of molecular features, antigen-binding, and in vitro properties of IgG and IgM variants of 4E10, an anti-HIV type 1 neutralizing monoclonal antibody.
AIDS research and human retroviruses 2004;20(7):755-62.
2004: Michael B Zwick; H Kiyomi Komori; Robyn L Stanfield; Sarah Church; Meng Wang; Paul W H I Parren; Renate Kunert; Hermann Katinger; Ian A Wilson; Dennis R Burton
The long third complementarity-determining region of the heavy chain is important in the activity of the broadly neutralizing anti-human immunodeficiency virus type 1 antibody 2F5.
Journal of virology 2004;78(6):3155-61.
2003: Daniel A Calarese; Christopher N Scanlan; Michael B Zwick; Songpon Deechongkit; Yusuke Mimura; Renate Kunert; Ping Zhu; Mark R Wormald; Robyn L Stanfield; Kenneth H Roux; Jeffery W Kelly; Pauline M Rudd; Raymond A Dwek; Hermann Katinger; Dennis R Burton; Ian A Wilson
Antibody domain exchange is an immunological solution to carbohydrate cluster recognition.
Science (New York, N.Y.) 2003;300(5628):2065-71.
2003: Susanne Wolbank; Renate Kunert; Gabriela Stiegler; Hermann Katinger
Characterization of human class-switched polymeric (immunoglobulin M [IgM] and IgA) anti-human immunodeficiency virus type 1 antibodies 2F5 and 2G12.
Journal of virology 2003;77(7):4095-103.
2002: Gabriela Stiegler; Christine Armbruster; Brigitta Vcelar; Heribert Stoiber; Renate Kunert; Nelson L Michael; Linda L Jagodzinski; Christoph G Ammann; Walter Jäger; Jeffrey Jacobson; Norbert Vetter; Hermann Katinger
Antiviral activity of the neutralizing antibodies 2F5 and 2G12 in asymptomatic HIV-1-infected humans: a phase I evaluation.
AIDS (London, England) 2002;16(15):2019-25.
2002: Renate Kunert; Robert Weik; Boris Ferko; Gabriela Stiegler; Hermann Katinger
Anti-idiotypic antibody Ab2/3H6 mimics the epitope of the neutralizing anti-HIV-1 monoclonal antibody 2F5.
AIDS (London, England) 2002;16(4):667-8.
2001: Gabriela Stiegler; Renate Kunert; M Purtscher; Susanne Wolbank; Regina Voglauer; Franz Steindl; Hermann Katinger
A potent cross-clade neutralizing human monoclonal antibody against a novel epitope on gp41 of human immunodeficiency virus type 1.
AIDS research and human retroviruses 2001;17(18):1757-65.
2001: Johannes Grillari; Klaus Fortschegger; Reingard Grabherr; O Hohenwarter; Renate Kunert; Hermann Katinger
Analysis of alterations in gene expression after amplification of recombinant genes in CHO cells.
Journal of biotechnology 2001;87(1):59-65.
2000: Renate Kunert; Willibald Steinfellner; M Purtscher; A Assadian; Hermann Katinger
Stable recombinant expression of the anti HIV-1 monoclonal antibody 2F5 after IgG3/IgG1 subclass switch in CHO cells.
Biotechnology and bioengineering 2000;67(1):97-103.
2000: Nicole Borth; M Zeyda; Renate Kunert; Hermann Katinger
Efficient selection of high-producing subclones during gene amplification of recombinant Chinese hamster ovary cells by flow cytometry and cell sorting.
Biotechnology and bioengineering 2000;71(4):266-73.
1999: M Zeyda; Nicole Borth; Renate Kunert; Hermann Katinger
Optimization of sorting conditions for the selection of stable, high-producing mammalian cell lines.
Biotechnology progress 1999;15(5):953-7.
1999: K Strutzenberger; Nicole Borth; Renate Kunert; Willibald Steinfellner; Hermann Katinger
Changes during subclone development and ageing of human antibody-producing recombinant CHO cells.
Journal of biotechnology 1999;69(2-3):215-26.
1999: Nicole Borth; K Strutzenberger; Renate Kunert; Willibald Steinfellner; Hermann Katinger
Analysis of changes during subclone development and ageing of human antibody-producing heterohybridoma cells by northern blot and flow cytometry.
Journal of biotechnology 1999;67(1):57-66.
1998: Renate Kunert; Florian Rüker; Hermann Katinger
Molecular characterization of five neutralizing anti-HIV type 1 antibodies: identification of nonconventional D segments in the human monoclonal antibodies 2G12 and 2F5.
AIDS research and human retroviruses 1998;14(13):1115-28.
1996: Nicole Borth; U Donalies; Renate Kunert; K Strutzenberger; Hermann Katinger
Comparison of the production of a human monoclonal antibody against HIV-1 by heterohybridoma cells and recombinant CHO cells: A flow cytometric study.
Cytotechnology 1996;22(1-3):129-38.