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Douglas Lauffenburger

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TOP 3
Tharathorn Rimchala; Roger D Kamm; Douglas A Lauffenburger
Endothelial cell phenotypic behaviors cluster into dynamic state transition programs modulated by angiogenic and angiostatic cytokines.
Ranjani K Paradise; Douglas A Lauffenburger; Matthew J Whitfield; Krystyn J Van Vliet
Directional cell migration in an extracellular pH gradient: a model study with an engineered cell line and primary microvascular endothelial cells.
Chia-Hung Chen; Linda G Griffith; Michael T Beste; Keith B Isaacson; Douglas A Lauffenburger; Miles A Miller; Aniruddh Sarkar; Jongyoon Han
Multiplexed protease activity assay for low-volume clinical samples using droplet-based microfluidics and its application to endometriosis.

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All Publications

2013: Tharathorn Rimchala; Roger D Kamm; Douglas A Lauffenburger
Endothelial cell phenotypic behaviors cluster into dynamic state transition programs modulated by angiogenic and angiostatic cytokines.
Integrative biology : quantitative biosciences from nano to macro 2013;5(3):510-22.
2013: Ranjani K Paradise; Douglas A Lauffenburger; Matthew J Whitfield; Krystyn J Van Vliet
Directional cell migration in an extracellular pH gradient: a model study with an engineered cell line and primary microvascular endothelial cells.
Experimental cell research 2013;319(4):487-97.
2013: Chia-Hung Chen; Linda G Griffith; Michael T Beste; Keith B Isaacson; Douglas A Lauffenburger; Miles A Miller; Aniruddh Sarkar; Jongyoon Han
Multiplexed protease activity assay for low-volume clinical samples using droplet-based microfluidics and its application to endometriosis.
Journal of the American Chemical Society 2013;135(5):1645-8.
2013: Shao-shan Carol Huang; Adam Labadorf; Douglas A Lauffenburger; Candace R Chouinard; David C Clarke; William Gordon; Sara J C Gosline; Ernest Fraenkel
Linking proteomic and transcriptional data through the interactome and epigenome reveals a map of oncogene-induced signaling.
PLoS computational biology 2013;9(2):e1002887.
2013: Justin R Pritchard; Douglas A Lauffenburger; Peter M Bruno; Kelsey L Capron; Luke A Gilbert; Michael T Hemann
Defining principles of combination drug mechanisms of action.
Proceedings of the National Academy of Sciences of the United States of America 2013;110(2):E170-9.
2013: Douglas A Lauffenburger
Our new look at biology.
Integrative biology : quantitative biosciences from nano to macro 2013;5(1):15-6.
2013: David C Clarke; Melody K Morris; Douglas A Lauffenburger
Normalization and statistical analysis of multiplexed bead-based immunoassay data using mixed-effects modeling.
Molecular & cellular proteomics : MCP 2013;12(1):245-62.
2013: Kelly F Benedict; Douglas A Lauffenburger
Insights into proteomic immune cell signaling and communication via data-driven modeling.
Current topics in microbiology and immunology 2013;363():201-33.
2012: Yvonne J Yamanaka; Gregory L Szeto; Kelly F Benedict; Mavis S N Brefo; Talitha L Forcier; Todd M Gierahn; Darrell J Irvine; Douglas A Lauffenburger; J Christopher Love
Cellular barcodes for efficiently profiling single-cell secretory responses by microengraving.
Analytical chemistry 2012;84(24):10531-6.
2012: Justin R Pritchard; Douglas A Lauffenburger; Michael T Hemann
Understanding resistance to combination chemotherapy.
Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 2012;15(5-6):249-57.
2012: Ericka M Noonan; Dharini Shah; Michael B Yaffe; Douglas A Lauffenburger; Leona D Samson
O6-Methylguanine DNA lesions induce an intra-S-phase arrest from which cells exit into apoptosis governed by early and late multi-pathway signaling network activation.
Integrative biology : quantitative biosciences from nano to macro 2012;4(10):1237-55.
2012: Kristen M Naegle; Forest M White; Douglas A Lauffenburger; Michael B Yaffe
Robust co-regulation of tyrosine phosphorylation sites on proteins reveals novel protein interactions.
Molecular bioSystems 2012;8(10):2771-82.
2012: Sarah E Kolitz; Douglas A Lauffenburger
Measurement and modeling of signaling at the single-cell level.
Biochemistry 2012;51(38):7433-43.
2012: Brian A Joughin; Douglas A Lauffenburger; Chengcheng Liu; Christopher W V Hogue; Michael B Yaffe
Protein kinases display minimal interpositional dependence on substrate sequence: potential implications for the evolution of signalling networks.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences 2012;367(1602):2574-83.
2012: Aaron S Meyer; Alan Wells; Amalchi Castillo; Frank B Gertler; Shannon K Hughes-Alford; Jennifer E Kay; Douglas A Lauffenburger
2D protrusion but not motility predicts growth factor-induced cancer cell migration in 3D collagen.
The Journal of cell biology 2012;197(6):721-9.
2012: Shannon K Hughes-Alford; Douglas A Lauffenburger
Quantitative analysis of gradient sensing: towards building predictive models of chemotaxis in cancer.
Current opinion in cell biology 2012;24(2):284-91.
2012: Melody K Morris; Ram Sasisekharan; Zachary Shriver; Douglas A Lauffenburger
Querying quantitative logic models (Q2LM) to study intracellular signaling networks and cell-cytokine interactions.
Biotechnology journal 2012;7(3):374-86.
2012: Cliff I Stains; Nathan C Tedford; Traci C Walkup; Brenda N Goguen; Linda G Griffith; Douglas A Lauffenburger; Elvedin Luković; Barbara Imperiali
Interrogating signaling nodes involved in cellular transformations using kinase activity probes.
Chemistry & biology 2012;19(2):210-7.
2012: David C Clarke; Douglas A Lauffenburger
Multi-pathway network analysis of mammalian epithelial cell responses in inflammatory environments.
Biochemical Society transactions 2012;40(1):133-8.
2012: Ta-Chun Hang; Douglas A Lauffenburger; Linda G Griffith; Donna B Stolz
Lipids promote survival, proliferation, and maintenance of differentiation of rat liver sinusoidal endothelial cells in vitro.
American journal of physiology. Gastrointestinal and liver physiology 2012;302(3):G375-88.
2012: Qing Han; Douglas A Lauffenburger; Neda Bagheri; Elizabeth M Bradshaw; David A Hafler; J Christopher Love
Polyfunctional responses by human T cells result from sequential release of cytokines.
Proceedings of the National Academy of Sciences of the United States of America 2012;109(5):1607-12.
2012: Alexander Mitsos; Melody K Morris; Julio Saez-Rodriguez; Douglas A Lauffenburger; Ioannis N Melas; Leonidas Alexopoulos
Non Linear Programming (NLP) formulation for quantitative modeling of protein signal transduction pathways.
PloS one 2012;7(11):e50085.
2012: Camille Terfve; Martijn van Iersel; Thomas Cokelaer; Emanuel Goncalves; David Henriques; Douglas A Lauffenburger; Aidan MacNamara; Melody K Morris; Julio Saez-Rodriguez
CellNOptR: a flexible toolkit to train protein signaling networks to data using multiple logic formalisms.
BMC systems biology 2012;6():133.
2012: Daniel C Kirouac; Douglas A Lauffenburger; Julio Saez-Rodriguez; John M Burke; Jennifer Swantek; Peter K Sorger
Creating and analyzing pathway and protein interaction compendia for modelling signal transduction networks.
BMC systems biology 2012;6():29.
2012: Andrea R Tentner; Douglas A Lauffenburger; Michael J Lee; Gerard J. Ostheimer; Leona D Samson; Michael B Yaffe
Combined experimental and computational analysis of DNA damage signaling reveals context-dependent roles for Erk in apoptosis and G1/S arrest after genotoxic stress.
Molecular systems biology 2012;8():568.
2011: Marcia L Moss; Robert Petrovich; Gary Powell; Bin Qi; Qing-Xiang Amy Sang; Uwe Schlomann; Mara Taverna; Ina Tesseur; Julia Li Zhong; Pei Zhou; Bart De Strooper; Colin Dingwall; Lori Edwards; Taheera Ferdous; Linda G Griffith; Douglas A Lauffenburger; Stefan F Lichtenthaler; Miles A Miller; Joerg Walter Bartsch
ADAM9 inhibition increases membrane activity of ADAM10 and controls α-secretase processing of amyloid precursor protein.
The Journal of biological chemistry 2011;286(47):40443-51.
2011: Hyung-Do Kim; Shannon K Alford; Frank B Gertler; Aaron S Meyer; Joel P Wagner; Alan Wells; Douglas A Lauffenburger
Signaling network state predicts twist-mediated effects on breast cell migration across diverse growth factor contexts.
Molecular & cellular proteomics : MCP 2011;10(11):M111.008433.
2011: Shan Wu; Alan Wells; Linda G Griffith; Douglas A Lauffenburger
Controlling multipotent stromal cell migration by integrating "course-graining" materials and "fine-tuning" small molecules via decision tree signal-response modeling.
Biomaterials 2011;32(30):7524-31.
2011: Laura B Kleiman; Douglas A Lauffenburger; Thomas Maiwald; Holger Conzelmann; Peter K Sorger
Rapid phospho-turnover by receptor tyrosine kinases impacts downstream signaling and drug binding.
Molecular cell 2011;43(5):723-37.
2011: Justin R Pritchard; Jennifer L Ricks; Luke A Gilbert; Hai Jiang; Douglas A Lauffenburger; Corbin E Meacham; Michael T Hemann
Bcl-2 family genetic profiling reveals microenvironment-specific determinants of chemotherapeutic response.
Cancer research 2011;71(17):5850-8.
2011: Julio Saez-Rodriguez; Leonidas G Alexopoulos; Douglas A Lauffenburger; Melody K Morris; Mingsheng Zhang; Peter K Sorger
Comparing signaling networks between normal and transformed hepatocytes using discrete logical models.
Cancer research 2011;71(16):5400-11.
2011: Chia-Hung Chen; Linda G Griffith; Sung Jae Kim; Douglas A Lauffenburger; Miles A Miller; Aniruddh Sarkar; Yong-Ak Song; Jongyoon Han
Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator.
Journal of the American Chemical Society 2011;133(27):10368-71.
2011: Kristen M Naegle; Roy E Welsch; Forest M White; Michael B Yaffe; Douglas A Lauffenburger
MCAM: multiple clustering analysis methodology for deriving hypotheses and insights from high-throughput proteomic datasets.
PLoS computational biology 2011;7(7):e1002119.
2011: Evanthia T Roussos; Jeffrey E Segall; Robert Stobezki; Yarong Wang; Jeffrey Bruce Wyckoff; Shannon K Alford; Michele Balsamo; Anne R Bresnick; Frank B Gertler; Bojana Gligorijevic; Sumanta Goswami; Douglas A Lauffenburger; Maria Pozzuto; John Condeelis
Mena invasive (MenaINV) promotes multicellular streaming motility and transendothelial migration in a mouse model of breast cancer.
Journal of cell science 2011;124(Pt 13):2120-31.
2011: Megan J Palmer; Vinay S Mahajan; Jianzhu Chen; Darrell J Irvine; Douglas A Lauffenburger
Signaling thresholds govern heterogeneity in IL-7-receptor-mediated responses of naïve CD8(+) T cells.
Immunology and cell biology 2011;89(5):581-94.
2011: Shelly R Peyton; Z Ilke Kalcioglu; Joshua C Cohen; Anne P Runkle; Krystyn J Van Vliet; Douglas A Lauffenburger; Linda G Griffith
Marrow-derived stem cell motility in 3D synthetic scaffold is governed by geometry along with adhesivity and stiffness.
Biotechnology and bioengineering 2011;108(5):1181-93.
2011: Miles A Miller; Layla Barkal; Karen Jeng; Andreas Herrlich; Marcia Moss; Linda G Griffith; Douglas A Lauffenburger
Proteolytic Activity Matrix Analysis (PrAMA) for simultaneous determination of multiple protease activities.
Integrative biology : quantitative biosciences from nano to macro 2011;3(4):422-38.
2011: Melody K Morris; Julio Saez-Rodriguez; David C Clarke; Peter K Sorger; Douglas A Lauffenburger
Training signaling pathway maps to biochemical data with constrained fuzzy logic: quantitative analysis of liver cell responses to inflammatory stimuli.
PLoS computational biology 2011;7(3):e1001099.
2011: Neda Bagheri; Marisa Shiina; Douglas A Lauffenburger; W Michael Korn
A dynamical systems model for combinatorial cancer therapy enhances oncolytic adenovirus efficacy by MEK-inhibition.
PLoS computational biology 2011;7(2):e1001085.
2011: Hai Jiang; Justin R Pritchard; Richard T Williams; Douglas A Lauffenburger; Michael T Hemann
A mammalian functional-genetic approach to characterizing cancer therapeutics.
Nature chemical biology 2011;7(2):92-100.
2011: Bree B Aldridge; Suzanne Gaudet; Douglas A Lauffenburger; Peter K Sorger
Lyapunov exponents and phase diagrams reveal multi-factorial control over TRAIL-induced apoptosis.
Molecular systems biology 2011;7():553.
2011: Ken S Lau; Alwin M Juchheim; Kimberly R Cavaliere; Sarah R Philips; Douglas A Lauffenburger; Kevin M Haigis
In vivo systems analysis identifies spatial and temporal aspects of the modulation of TNF-α-induced apoptosis and proliferation by MAPKs.
Science signaling 2011;4(165):ra16.
2011: Ranjani K Paradise; Douglas A Lauffenburger; Krystyn J Van Vliet
Acidic extracellular pH promotes activation of integrin α(v)β(3).
PloS one 2011;6(1):e15746.
2011: Alan Wells; Yvonne L Chao; Jelena Grahovac; Qian Wu; Douglas A Lauffenburger
Epithelial and mesenchymal phenotypic switchings modulate cell motility in metastasis.
Frontiers in bioscience : a journal and virtual library 2011;16():815-37.
2010: Kristen M Naegle; Melissa Gymrek; Brian A Joughin; Joel P Wagner; Roy E Welsch; Michael B Yaffe; Douglas A Lauffenburger; Forest M White
PTMScout, a Web resource for analysis of high throughput post-translational proteomics studies.
Molecular & cellular proteomics : MCP 2010;9(11):2558-70.
2010: Christopher W Espelin; Arthur Goldsipe; Peter K Sorger; Douglas A Lauffenburger; David de Graaf; Bart S Hendriks
Elevated GM-CSF and IL-1beta levels compromise the ability of p38 MAPK inhibitors to modulate TNFalpha levels in the human monocytic/macrophage U937 cell line.
Molecular bioSystems 2010;6(10):1956-72.
2010: Leonidas Alexopoulos; Julio Saez-Rodriguez; Benjamin D Cosgrove; Douglas A Lauffenburger; Peter K Sorger
Networks inferred from biochemical data reveal profound differences in toll-like receptor and inflammatory signaling between normal and transformed hepatocytes.
Molecular & cellular proteomics : MCP 2010;9(9):1849-65.
2010: Jamie B Spangler; Jason R Neil; Sivan Abramovitch; Yosef Yarden; Forest M White; Douglas A Lauffenburger; K Dane Wittrup
Combination antibody treatment down-regulates epidermal growth factor receptor by inhibiting endosomal recycling.
Proceedings of the National Academy of Sciences of the United States of America 2010;107(30):13252-7.
2010: Robin N Prince; Eric R Schreiter; Peng Zou; H Steven Wiley; Alice Y Ting; Richard T Lee; Douglas A Lauffenburger
The heparin-binding domain of HB-EGF mediates localization to sites of cell-cell contact and prevents HB-EGF proteolytic release.
Journal of cell science 2010;123(Pt 13):2308-18.
2010: Elizabeth J Joslin; Harish Shankaran; Lee K Opresko; Nikki Bollinger; Douglas A Lauffenburger; H Steven Wiley
Structure of the EGF receptor transactivation circuit integrates multiple signals with cell context.
Molecular bioSystems 2010;6(7):1293-306.
2010: Benjamin D Cosgrove; Leonidas Alexopoulos; Ta-chun Hang; Bart S Hendriks; Peter K Sorger; Linda G Griffith; Douglas A Lauffenburger
Cytokine-associated drug toxicity in human hepatocytes is associated with signaling network dysregulation.
Molecular bioSystems 2010;6(7):1195-206.
2010: Matthew J Lazzara; Keara Lane; Richard Chan; Paul J Jasper; Michael B Yaffe; Peter K Sorger; Tyler Jacks; Benjamin G Neel; Douglas A Lauffenburger
Impaired SHP2-mediated extracellular signal-regulated kinase activation contributes to gefitinib sensitivity of lung cancer cells with epidermal growth factor receptor-activating mutations.
Cancer research 2010;70(9):3843-50.
2010: Melody K Morris; Julio Saez-Rodriguez; Peter K Sorger; Douglas A Lauffenburger
Logic-based models for the analysis of cell signaling networks.
Biochemistry 2010;49(15):3216-24.
2010: Pamela K Kreeger; Yufang Wang; Kevin M Haigis; Douglas A Lauffenburger
Integration of multiple signaling pathway activities resolves K-RAS/N-RAS mutation paradox in colon epithelial cell response to inflammatory cytokine stimulation.
Integrative biology : quantitative biosciences from nano to macro 2010;2(4):202-8.
2010: Mark F Ciaccio; Joel P Wagner; Chih-Pin Chuu; Douglas A Lauffenburger; Richard B Jones
Systems analysis of EGF receptor signaling dynamics with microwestern arrays.
Nature methods 2010;7(2):148-55.
2010: Pamela K Kreeger; Douglas A Lauffenburger
Cancer systems biology: a network modeling perspective.
Carcinogenesis 2010;31(1):2-8.
2009: Manu Platt; Catera L Wilder; Alan Wells; Linda G Griffith; Douglas A Lauffenburger
Multipathway kinase signatures of multipotent stromal cells are predictive for osteogenic differentiation: tissue-specific stem cells.
Stem cells (Dayton, Ohio) 2009;27(11):2804-14.
2009: Manu Platt; Arian J Roman; Alan Wells; Douglas A Lauffenburger; Linda G Griffith
Sustained epidermal growth factor receptor levels and activation by tethered ligand binding enhances osteogenic differentiation of multi-potent marrow stromal cells.
Journal of cellular physiology 2009;221(2):306-17.
2009: Pamela K Kreeger; Roli Mandhana; Shannon Alford; Kevin Haigis; Douglas A Lauffenburger
RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects.
Cancer research 2009;69(20):8191-9.
2009: Jeong Hoon Lee; Benjamin D Cosgrove; Douglas A Lauffenburger; Jongyoon Han
Microfluidic concentration-enhanced cellular kinase activity assay.
Journal of the American Chemical Society 2009;131(30):10340-1.
2009: Justin R Pritchard; Benjamin D Cosgrove; Michael T Hemann; Linda G Griffith; Jack R Wands; Douglas A Lauffenburger
Three-kinase inhibitor combination recreates multipathway effects of a geldanamycin analogue on hepatocellular carcinoma cell death.
Molecular cancer therapeutics 2009;8(8):2183-92.
2009: Benjamin D Cosgrove; Bracken M King; Maya A Hasan; Leonidas Alexopoulos; Paraskevi A Farazi; Bart S Hendriks; Linda G Griffith; Peter K Sorger; Bruce Tidor; Jinghai J Xu; Douglas A Lauffenburger
Synergistic drug-cytokine induction of hepatocellular death as an in vitro approach for the study of inflammation-associated idiosyncratic drug hepatotoxicity.
Toxicology and applied pharmacology 2009;237(3):317-30.
2009: Bree B Aldridge; Julio Saez-Rodriguez; Jeremy L Muhlich; Peter K Sorger; Douglas A Lauffenburger
Fuzzy logic analysis of kinase pathway crosstalk in TNF/EGF/insulin-induced signaling.
PLoS computational biology 2009;5(4):e1000340.
2009: Matthew J Lazzara; Douglas A Lauffenburger
Quantitative modeling perspectives on the ErbB system of cell regulatory processes.
Experimental cell research 2009;315(4):717-25.
2009: Karen Sachs; Solomon Itani; Jennifer Carlisle; Garry P Nolan; Dana Pe'er; Douglas A Lauffenburger
Learning signaling network structures with sparsely distributed data.
Journal of computational biology : a journal of computational molecular cell biology 2009;16(2):201-12.
2009: Benjamin D Cosgrove; Leonidas Alexopoulos; Julio Saez-Rodriguez; Linda G Griffith; Douglas A Lauffenburger
A multipathway phosphoproteomic signaling network model of idiosyncratic drug- and inflammatory cytokine-induced toxicity in human hepatocytes.
Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference 2009;2009():5452-5.
2009: Julio Saez-Rodriguez; Leonidas G Alexopoulos; Jonathan Epperlein; Regina Samaga; Douglas A Lauffenburger; Steffen Klamt; Peter K Sorger
Discrete logic modelling as a means to link protein signalling networks with functional analysis of mammalian signal transduction.
Molecular systems biology 2009;5():331.
2009: Dominik M Wittmann; Jan Krumsiek; Julio Saez-Rodriguez; Douglas A Lauffenburger; Steffen Klamt; Fabian J Theis
Transforming Boolean models to continuous models: methodology and application to T-cell receptor signaling.
BMC systems biology 2009;3():98.
2009: William W Chen; Birgit Schoeberl; Paul J Jasper; Mario Niepel; Ulrik B Nielsen; Douglas A Lauffenburger; Peter K Sorger
Input-output behavior of ErbB signaling pathways as revealed by a mass action model trained against dynamic data.
Molecular systems biology 2009;5():239.
2008: John G Albeck; John M Burke; Sabrina L Spencer; Douglas A Lauffenburger; Peter K Sorger
Modeling a snap-action, variable-delay switch controlling extrinsic cell death.
PLoS biology 2008;6(12):2831-52.
2008: Hyung-Do Kim; Tiffany W Guo; Angela P Wu; Alan Wells; Frank B Gertler; Douglas A Lauffenburger
Epidermal growth factor-induced enhancement of glioblastoma cell migration in 3D arises from an intrinsic increase in speed but an extrinsic matrix- and proteolysis-dependent increase in persistence.
Molecular biology of the cell 2008;19(10):4249-59.
2008: Brendan A Harley; Hyung-Do Kim; Muhammad H Zaman; Ioannis V Yannas; Douglas A Lauffenburger; Lorna J Gibson
Microarchitecture of three-dimensional scaffolds influences cell migration behavior via junction interactions.
Biophysical journal 2008;95(8):4013-24.
2008: Benjamin D Cosgrove; Connie Cheng; Justin R Pritchard; Donna B Stolz; Douglas A Lauffenburger; Linda G Griffith
An inducible autocrine cascade regulates rat hepatocyte proliferation and apoptosis responses to tumor necrosis factor-alpha.
Hepatology (Baltimore, Md.) 2008;48(1):276-88.
2008: Neil Kumar; Raffi Afeyan; Hyung-Do Kim; Douglas A Lauffenburger
Multipathway model enables prediction of kinase inhibitor cross-talk effects on migration of Her2-overexpressing mammary epithelial cells.
Molecular pharmacology 2008;73(6):1668-78.
2008: John G Albeck; John M Burke; Bree B Aldridge; Mingsheng Zhang; Douglas A Lauffenburger; Peter K Sorger
Quantitative analysis of pathways controlling extrinsic apoptosis in single cells.
Molecular cell 2008;30(1):11-25.
2008: Alice Macdonald; Alan Rick Horwitz; Douglas A Lauffenburger
Kinetic model for lamellipodal actin-integrin 'clutch' dynamics.
Cell adhesion & migration 2008;2(2):95-105.
2008: Megan J Palmer; Vinay S Mahajan; Lily C Trajman; Darrell J Irvine; Douglas A Lauffenburger; Jianzhu Chen
Interleukin-7 receptor signaling network: an integrated systems perspective.
Cellular & molecular immunology 2008;5(2):79-89.
2008: Julio Saez-Rodriguez; Arthur Goldsipe; Jeremy Muhlich; Leonidas Alexopoulos; Bjorn Millard; Douglas A Lauffenburger; Peter K Sorger
Flexible informatics for linking experimental data to mathematical models via DataRail.
Bioinformatics (Oxford, England) 2008;24(6):840-7.
2007: Gregory T Zugates; Nathan C Tedford; Andreas Zumbuehl; Siddharth Jhunjhunwala; Christina S Kang; Linda G Griffith; Douglas A Lauffenburger; Robert Langer; Daniel G Anderson
Gene delivery properties of end-modified poly(beta-amino ester)s.
Bioconjugate chemistry 2007;18(6):1887-96.
2007: Elizabeth J Joslin; Lee K Opresko; Alan Wells; H Steven Wiley; Douglas A Lauffenburger
EGF-receptor-mediated mammary epithelial cell migration is driven by sustained ERK signaling from autocrine stimulation.
Journal of cell science 2007;120(Pt 20):3688-99.
2007: Kathryn Miller-Jensen; Kevin Janes; Joan S Brugge; Douglas A Lauffenburger
Common effector processing mediates cell-specific responses to stimuli.
Nature 2007;448(7153):604-8.
2007: Lucia Wille; Melissa L Kemp; Peter Sandy; Christina L Lewis; Douglas A Lauffenburger
Epi-allelic Erk1 and Erk2 knockdown series for quantitative analysis of T cell Erk regulation and IL-2 production.
Molecular immunology 2007;44(12):3085-91.
2007: Melissa L Kemp; Lucia Wille; Christina L Lewis; Lindsay B Nicholson; Douglas A Lauffenburger
Quantitative network signal combinations downstream of TCR activation can predict IL-2 production response.
Journal of immunology (Baltimore, Md. : 1950) 2007;178(8):4984-92.
2007: Alejandro Wolf-Yadlin; Sampsa Hautaniemi; Douglas A Lauffenburger; Forest M White
Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(14):5860-5.
2007: Neil Kumar; Raffi Afeyan; Sarah Sheppard; Brian Harms; Douglas A Lauffenburger
Quantitative analysis of Akt phosphorylation and activity in response to EGF and insulin treatment.
Biochemical and biophysical research communications 2007;354(1):14-20.
2007: Neil Kumar; Alejandro Wolf-Yadlin; Forest M White; Douglas A Lauffenburger
Modeling HER2 effects on cell behavior from mass spectrometry phosphotyrosine data.
PLoS computational biology 2007;3(1):e4.
2007: Sourabh Kharait; Sampsa Hautaniemi; Shan Wu; Akihiro Iwabu; Douglas A Lauffenburger; Alan Wells
Decision tree modeling predicts effects of inhibiting contractility signaling on cell motility.
BMC systems biology 2007;1():9.
2007: Muhammad H Zaman; Paul Matsudaira; Douglas A Lauffenburger
Understanding effects of matrix protease and matrix organization on directional persistence and translational speed in three-dimensional cell migration.
Annals of biomedical engineering 2007;35(1):91-100.
2006: Bart S Hendriks; G J Griffiths; R Benson; D Kenyon; M Lazzara; J Swinton; S Beck; M Hickinson; J M Beusmans; Douglas A Lauffenburger; David de Graaf
Decreased internalisation of erbB1 mutants in lung cancer is linked with a mechanism conferring sensitivity to gefitinib.
Systems biology 2006;153(6):457-66.
2006: Bree B Aldridge; G Haller; Peter K Sorger; Douglas A Lauffenburger
Direct Lyapunov exponent analysis enables parametric study of transient signalling governing cell behaviour.
Systems biology 2006;153(6):425-32.
2006: Bree B Aldridge; John M Burke; Douglas A Lauffenburger; Peter K Sorger
Physicochemical modelling of cell signalling pathways.
Nature cell biology 2006;8(11):1195-203.
2006: John G Albeck; Gavin MacBeath; Forest M White; Peter K Sorger; Douglas A Lauffenburger; Suzanne Gaudet
Collecting and organizing systematic sets of protein data.
Nature reviews. Molecular cell biology 2006;7(11):803-12.
2006: Douglas A Lauffenburger
Right on cue.
Nature chemical biology 2006;2(11):569-70.
2006: Kathryn Miller-Jensen; Kevin Janes; Yun-Ling Wong; Linda G Griffith; Douglas A Lauffenburger
Adenoviral vector saturates Akt pro-survival signaling and blocks insulin-mediated rescue of tumor necrosis-factor-induced apoptosis.
Journal of cell science 2006;119(Pt 18):3788-98.
2006: Neil Kumar; Bart S Hendriks; Kevin Janes; David de Graaf; Douglas A Lauffenburger
Applying computational modeling to drug discovery and development.
Drug discovery today 2006;11(17-18):806-11.
2006: Fei Hua; Sampsa Hautaniemi; Rayka Yokoo; Douglas A Lauffenburger
Integrated mechanistic and data-driven modelling for multivariate analysis of signalling pathways.
Journal of the Royal Society, Interface / the Royal Society 2006;3(9):515-26.
2006: Neil Kumar; Muhammad H Zaman; Hyung-Do Kim; Douglas A Lauffenburger
A high-throughput migration assay reveals HER2-mediated cell migration arising from increased directional persistence.
Biophysical journal 2006;91(4):L32-4.
2006: Muhammad H Zaman; Linda M Trapani; Alisha L Sieminski; Alisha Siemeski; Drew Mackellar; Haiyan Gong; Roger Kamm; Alan Wells; Douglas A Lauffenburger; Paul Matsudaira
Migration of tumor cells in 3D matrices is governed by matrix stiffness along with cell-matrix adhesion and proteolysis.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(29):10889-94.
2006: Trey Ideker; L Raimond Winslow; Douglas A Lauffenburger
Bioengineering and systems biology.
Annals of biomedical engineering 2006;34(7):1226-33.
2006: Kirsty D Smith; Alan Wells; Douglas A Lauffenburger
Multiple signaling pathways mediate compaction of collagen matrices by EGF-stimulated fibroblasts.
Experimental cell research 2006;312(11):1970-82.
2006: Sourabh Kharait; Rajiv Dhir; Douglas A Lauffenburger; Alan Wells
Protein kinase Cdelta signaling downstream of the EGF receptor mediates migration and invasiveness of prostate cancer cells.
Biochemical and biophysical research communications 2006;343(3):848-56.
2006: Kevin Janes; Suzanne Gaudet; John G Albeck; Ulrik B Nielsen; Douglas A Lauffenburger; Peter K Sorger
The response of human epithelial cells to TNF involves an inducible autocrine cascade.
Cell 2006;124(6):1225-39.
2006: Kevin Janes; Douglas A Lauffenburger
A biological approach to computational models of proteomic networks.
Current opinion in chemical biology 2006;10(1):73-80.
2006: Carlos E Semino; Roger Kamm; Douglas A Lauffenburger
Autocrine EGF receptor activation mediates endothelial cell migration and vascular morphogenesis induced by VEGF under interstitial flow.
Experimental cell research 2006;312(3):289-98.
2006: Alejandro Wolf-Yadlin; Neil Kumar; Yi Zhang; Sampsa Hautaniemi; Muhammad Zaman; Hyung-Do Kim; Viara Grantcharova; Douglas A Lauffenburger; Forest M White
Effects of HER2 overexpression on cell signaling networks governing proliferation and migration.
Molecular systems biology 2006;2():54.
2006: Bart S Hendriks; J Cook; J M Burke; J M Beusmans; Douglas A Lauffenburger; David de Graaf
Computational modelling of ErbB family phosphorylation dynamics in response to transforming growth factor alpha and heregulin indicates spatial compartmentation of phosphatase activity.
Systems biology 2006;153(1):22-33.
2006: Alan Wells; Brian Harms; Akihiro Iwabu; Lily Koo; Kirsty Smith; Linda Griffith; Douglas A Lauffenburger
Motility signaled from the EGF receptor and related systems.
Methods in molecular biology (Clifton, N.J.) 2006;327():159-77.
2005: Daniel T Kamei; Bert J Lao; Margaret Speed Ricci; Rohini Deshpande; Han Xu; Bruce Tidor; Douglas A Lauffenburger
Quantitative methods for developing Fc mutants with extended half-lives.
Biotechnology and bioengineering 2005;92(6):748-60.
2005: Kevin Janes; John G Albeck; Suzanne Gaudet; Peter K Sorger; Douglas A Lauffenburger; Michael B Yaffe
A systems model of signaling identifies a molecular basis set for cytokine-induced apoptosis.
Science (New York, N.Y.) 2005;310(5754):1646-53.
2005: Suzanne Gaudet; Kevin Janes; John G Albeck; Emily A Pace; Douglas A Lauffenburger; Peter K Sorger
A compendium of signals and responses triggered by prodeath and prosurvival cytokines.
Molecular & cellular proteomics : MCP 2005;4(10):1569-90.
2005: Sowmya Viswanathan; Ryan E Davey; Donavan Cheng; Riyad C Raghu; Douglas A Lauffenburger; Peter W Zandstra
Clonal evolution of stem and differentiated cells can be predicted by integrating cell-intrinsic and -extrinsic parameters.
Biotechnology and applied biochemistry 2005;42(Pt 2):119-31.
2005: Yi Zhang; Alejandro Wolf-Yadlin; Phillip L Ross; Darryl Pappin; John Rush; Douglas A Lauffenburger; Forest M White
Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules.
Molecular & cellular proteomics : MCP 2005;4(9):1240-50.
2005: Balaji M Rao; Ian Driver; Douglas A Lauffenburger; K Dane Wittrup
High-affinity CD25-binding IL-2 mutants potently stimulate persistent T cell growth.
Biochemistry 2005;44(31):10696-701.
2005: Muhammad H Zaman; Roger Kamm; Paul Matsudaira; Douglas A Lauffenburger
Computational model for cell migration in three-dimensional matrices.
Biophysical journal 2005;89(2):1389-97.
2005: Jun Yoshioka; Robin N Prince; Hayden Huang; Scott B Perkins; Francisco U Cruz; Catherine MacGillivray; Douglas A Lauffenburger; Richard T Lee
Cardiomyocyte hypertrophy and degradation of connexin43 through spatially restricted autocrine/paracrine heparin-binding EGF.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(30):10622-7.
2005: Fei Hua; Melanie G Cornejo; Mike H Cardone; Cynthia L Stokes; Douglas A Lauffenburger
Effects of Bcl-2 levels on Fas signaling-induced caspase-3 activation: molecular genetic tests of computational model predictions.
Journal of immunology (Baltimore, Md. : 1950) 2005;175(2):985-95.
2005: Jianying Dong; Lee K Opresko; William Chrisler; Galya Orr; Ryan D Quesenberry; Douglas A Lauffenburger; H Steven Wiley
The membrane-anchoring domain of epidermal growth factor receptor ligands dictates their ability to operate in juxtacrine mode.
Molecular biology of the cell 2005;16(6):2984-98.
2005: Sampsa Hautaniemi; Sourabh Kharait; Akihiro Iwabu; Alan Wells; Douglas A Lauffenburger
Modeling of signal-response cascades using decision tree analysis.
Bioinformatics (Oxford, England) 2005;21(9):2027-35.
2005: Karen Sachs; Omar Perez; Dana Pe'er; Douglas A Lauffenburger; Garry P Nolan
Causal protein-signaling networks derived from multiparameter single-cell data.
Science (New York, N.Y.) 2005;308(5721):523-9.
2005: Melissa D Shults; Kevin Janes; Douglas A Lauffenburger; Barbara Imperiali
A multiplexed homogeneous fluorescence-based assay for protein kinase activity in cell lysates.
Nature methods 2005;2(4):277-83.
2005: Michael I Monine; Alexander M Berezhkovskii; Elizabeth J Joslin; H Steven Wiley; Douglas A Lauffenburger; Stanislav Y Shvartsman
Ligand accumulation in autocrine cell cultures.
Biophysical journal 2005;88(4):2384-90.
2005: Peter J Woolf; Wendy Prudhomme; Laurence Daheron; George Q Daley; Douglas A Lauffenburger
Bayesian analysis of signaling networks governing embryonic stem cell fate decisions.
Bioinformatics (Oxford, England) 2005;21(6):741-53.
2005: Bart S Hendriks; Galya Orr; Alan Wells; H Steven Wiley; Douglas A Lauffenburger
Parsing ERK activation reveals quantitatively equivalent contributions from epidermal growth factor receptor and HER2 in human mammary epithelial cells.
The Journal of biological chemistry 2005;280(7):6157-69.
2005: Brian D Harms; Gina M Bassi; Alan Rick Horwitz; Douglas A Lauffenburger
Directional persistence of EGF-induced cell migration is associated with stabilization of lamellipodial protrusions.
Biophysical journal 2005;88(2):1479-88.
2005: Balaji M Rao; Douglas A Lauffenburger; K Dane Wittrup
Integrating cell-level kinetic modeling into the design of engineered protein therapeutics.
Nature biotechnology 2005;23(2):191-4.
2005: Alan Wells; Anna Huttenlocher; Douglas A Lauffenburger
Calpain proteases in cell adhesion and motility.
International review of cytology 2005;245():1-16.
2004: Maya R Said; Thomas Begley; Alan V Oppenheim; Douglas A Lauffenburger; Leona D Samson
Global network analysis of phenotypic effects: protein networks and toxicity modulation in Saccharomyces cerevisiae.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(52):18006-11.
2004: Cheri S Lazar; Catherine M Cresson; Douglas A Lauffenburger; Gordon N Gill
The Na+/H+ exchanger regulatory factor stabilizes epidermal growth factor receptors at the cell surface.
Molecular biology of the cell 2004;15(12):5470-80.
2004: Wendy A Prudhomme; Keith H Duggar; Douglas A Lauffenburger
Cell population dynamics model for deconvolution of murine embryonic stem cell self-renewal and differentiation responses to cytokines and extracellular matrix.
Biotechnology and bioengineering 2004;88(3):264-72.
2004: Ivan V Maly; Richard T Lee; Douglas A Lauffenburger
A model for mechanotransduction in cardiac muscle: effects of extracellular matrix deformation on autocrine signaling.
Annals of biomedical engineering 2004;32(10):1319-35.
2004: Balaji M Rao; Ian Driver; Douglas A Lauffenburger; K Dane Wittrup
Interleukin 2 (IL-2) variants engineered for increased IL-2 receptor alpha-subunit affinity exhibit increased potency arising from a cell surface ligand reservoir effect.
Molecular pharmacology 2004;66(4):864-9.
2004: Hang Lu; Lily Y Koo; Wechung M Wang; Douglas A Lauffenburger; Linda G Griffith; Klavs F Jensen
Microfluidic shear devices for quantitative analysis of cell adhesion.
Analytical chemistry 2004;76(18):5257-64.
2004: Daniel J Tschumperlin; Guohao Dai; Ivan V Maly; Tadashi Kikuchi; Lily H Laiho; Anna K McVittie; Kathleen J Haley; Craig M Lilly; Peter So; Douglas A Lauffenburger; Roger Kamm; Jeffrey M Drazen
Mechanotransduction through growth-factor shedding into the extracellular space.
Nature 2004;429(6987):83-6.
2004: Akihiro Iwabu; Kirsty Smith; Fred D Allen; Douglas A Lauffenburger; Alan Wells
Epidermal growth factor induces fibroblast contractility and motility via a protein kinase C delta-dependent pathway.
The Journal of biological chemistry 2004;279(15):14551-60.
2004: Wendy Prudhomme; George Q Daley; Peter Zandstra; Douglas A Lauffenburger
Multivariate proteomic analysis of murine embryonic stem cell self-renewal versus differentiation signaling.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(9):2900-5.
2004: Douglas A Lauffenburger
Biological engineering and systems biology--new opportunities for engineers in the pharmaceutical industry.
Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference 2004;7():5456.
2004: Kevin Janes; Jason R Kelly; Suzanne Gaudet; John G Albeck; Peter K Sorger; Douglas A Lauffenburger
Cue-signal-response analysis of TNF-induced apoptosis by partial least squares regression of dynamic multivariate data.
Journal of computational biology : a journal of computational molecular cell biology 2004;11(4):544-61.
2004: Ivan V Maly; H Steven Wiley; Douglas A Lauffenburger
Self-organization of polarized cell signaling via autocrine circuits: computational model analysis.
Biophysical journal 2004;86(1 Pt 1):10-22.
2003: Sowmya Viswanathan; Tania Benatar; Maria Mileikovsky; Douglas A Lauffenburger; Andras Nagy; Peter W Zandstra
Supplementation-dependent differences in the rates of embryonic stem cell self-renewal, differentiation, and apoptosis.
Biotechnology and bioengineering 2003;84(5):505-17.
2003: Balaji M Rao; Andrew T Girvin; Thomas Ciardelli; Douglas A Lauffenburger; K Dane Wittrup
Interleukin-2 mutants with enhanced alpha-receptor subunit binding affinity.
Protein engineering 2003;16(12):1081-7.
2003: Bart S Hendriks; H Steven Wiley; Douglas A Lauffenburger
HER2-mediated effects on EGFR endosomal sorting: analysis of biophysical mechanisms.
Biophysical journal 2003;85(4):2732-45.
2003: Asmaa Mamoune; Jian-Hua Luo; Douglas A Lauffenburger; Alan Wells
Calpain-2 as a target for limiting prostate cancer invasion.
Cancer research 2003;63(15):4632-40.
2003: Kevin Janes; John G Albeck; Lili X Peng; Peter K Sorger; Douglas A Lauffenburger; Michael B Yaffe
A high-throughput quantitative multiplex kinase assay for monitoring information flow in signaling networks: application to sepsis-apoptosis.
Molecular & cellular proteomics : MCP 2003;2(7):463-73.
2003: Bart S Hendriks; Lee K Opresko; H Steven Wiley; Douglas A Lauffenburger
Quantitative analysis of HER2-mediated effects on HER2 and epidermal growth factor receptor endocytosis: distribution of homo- and heterodimers depends on relative HER2 levels.
The Journal of biological chemistry 2003;278(26):23343-51.
2003: Trey Ideker; Douglas A Lauffenburger
Building with a scaffold: emerging strategies for high- to low-level cellular modeling.
Trends in biotechnology 2003;21(6):255-62.
2003: Douglas A Lauffenburger; Alan Wells
Quantitative parsing of cell multi-tasking in wound repair and tissue morphogenesis.
Biophysical journal 2003;84(6):3499-500.
2003: Casim A Sarkar; Ky Lowenhaupt; Peggy J Wang; Thomas Horan; Douglas A Lauffenburger
Parsing the effects of binding, signaling, and trafficking on the mitogenic potencies of granulocyte colony-stimulating factor analogues.
Biotechnology progress 2003;19(3):955-64.
2003: Margaret Speed Ricci; Casim A Sarkar; Eric M Fallon; Douglas A Lauffenburger; David N Brems
pH Dependence of structural stability of interleukin-2 and granulocyte colony-stimulating factor.
Protein science : a publication of the Protein Society 2003;12(5):1030-8.
2003: Bart S Hendriks; Lee K Opresko; H Steven Wiley; Douglas A Lauffenburger
Coregulation of epidermal growth factor receptor/human epidermal growth factor receptor 2 (HER2) levels and locations: quantitative analysis of HER2 overexpression effects.
Cancer research 2003;63(5):1130-7.
2003: Casim A Sarkar; Douglas A Lauffenburger
Cell-level pharmacokinetic model of granulocyte colony-stimulating factor: implications for ligand lifetime and potency in vivo.
Molecular pharmacology 2003;63(1):147-58.
2003: H Steven Wiley; Stanislav Y Shvartsman; Douglas A Lauffenburger
Computational modeling of the EGF-receptor system: a paradigm for systems biology.
Trends in cell biology 2003;13(1):43-50.
2002: Ann DeWitt; Tomoko Iida; Ho-Yan Lam; Virginia Hill; H Steven Wiley; Douglas A Lauffenburger
Affinity regulates spatial range of EGF receptor autocrine ligand binding.
Developmental biology 2002;250(2):305-16.
2002: Karen Sachs; David Gifford; Tommi S Jaakkola; Peter Sorger; Douglas A Lauffenburger
Bayesian network approach to cell signaling pathway modeling.
Science's STKE : signal transduction knowledge environment 2002;2002(148):pe38.
2002: Casim A Sarkar; Ky Lowenhaupt; Thomas Horan; Thomas C Boone; Bruce Tidor; Douglas A Lauffenburger
Rational cytokine design for increased lifetime and enhanced potency using pH-activated "histidine switching".
Nature biotechnology 2002;20(9):908-13.
2002: Stanislav Y Shvartsman; Cyrill B Muratov; Douglas A Lauffenburger
Modeling and computational analysis of EGF receptor-mediated cell communication in Drosophila oogenesis.
Development (Cambridge, England) 2002;129(11):2577-89.
2002: Lily Y Koo; Darrell J Irvine; Anne M Mayes; Douglas A Lauffenburger; Linda G Griffith
Co-regulation of cell adhesion by nanoscale RGD organization and mechanical stimulus.
Journal of cell science 2002;115(Pt 7):1423-33.
2002: Stanislav Y Shvartsman; M P Hagan; Adly Yacoub; P Dent; H Steven Wiley; Douglas A Lauffenburger
Autocrine loops with positive feedback enable context-dependent cell signaling.
American journal of physiology. Cell physiology 2002;282(3):C545-59.
2002: Michael R Caplan; Elissa M Schwartzfarb; Shuguang Zhang; Roger Kamm; Douglas A Lauffenburger
Effects of systematic variation of amino acid sequence on the mechanical properties of a self-assembling, oligopeptide biomaterial.
Journal of biomaterials science. Polymer edition 2002;13(3):225-36.
2002: Alan Wells; Jareer Kassis; James Solava; Timothy Turner; Douglas A Lauffenburger
Growth factor-induced cell motility in tumor invasion.
Acta oncologica (Stockholm, Sweden) 2002;41(2):124-30.
2002: Fred D Allen; Clara F Asnes; Philip Chang; Elliot L Elson; Douglas A Lauffenburger; Alan Wells
Epidermal growth factor induces acute matrix contraction and subsequent calpain-modulated relaxation.
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 2002;10(1):67-76.
2002: Angela Glading; Douglas A Lauffenburger; Alan Wells
Cutting to the chase: calpain proteases in cell motility.
Trends in cell biology 2002;12(1):46-54.
2002: Michael R Caplan; Elissa M Schwartzfarb; Shuguang Zhang; Roger Kamm; Douglas A Lauffenburger
Control of self-assembling oligopeptide matrix formation through systematic variation of amino acid sequence.
Biomaterials 2002;23(1):219-27.
2001: G Maheshwari; H Steven Wiley; Douglas A Lauffenburger
Autocrine epidermal growth factor signaling stimulates directionally persistent mammary epithelial cell migration.
The Journal of cell biology 2001;155(7):1123-8.
2001: Stanislav Y Shvartsman; H Steven Wiley; William M Deen; Douglas A Lauffenburger
Spatial range of autocrine signaling: modeling and computational analysis.
Biophysical journal 2001;81(4):1854-67.
2001: Atul Narang; K K Subramanian; Douglas A Lauffenburger
A mathematical model for chemoattractant gradient sensing based on receptor-regulated membrane phospholipid signaling dynamics.
Annals of biomedical engineering 2001;29(8):677-91.
2001: C E Orsello; Douglas A Lauffenburger; D A Hammer
Molecular properties in cell adhesion: a physical and engineering perspective.
Trends in biotechnology 2001;19(8):310-6.
2001: Angela Glading; Florian Uberall; S M Keyse; Douglas A Lauffenburger; Alan Wells
Membrane proximal ERK signaling is required for M-calpain activation downstream of epidermal growth factor receptor signaling.
The Journal of biological chemistry 2001;276(26):23341-8.
2001: A E DeWitt; J Y Dong; H Steven Wiley; Douglas A Lauffenburger
Quantitative analysis of the EGF receptor autocrine system reveals cryptic regulation of cell response by ligand capture.
Journal of cell science 2001;114(Pt 12):2301-13.
2001: Douglas A Lauffenburger; Linda G Griffith
Who's got pull around here? Cell organization in development and tissue engineering.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(8):4282-4.
2001: Jareer Kassis; Douglas A Lauffenburger; Timothy Turner; Alan Wells
Tumor invasion as dysregulated cell motility.
Seminars in cancer biology 2001;11(2):105-17.
2001: AR Asthagiri; Douglas A Lauffenburger
A computational study of feedback effects on signal dynamics in a mitogen-activated protein kinase (MAPK) pathway model.
Biotechnology progress 2001;17(2):227-39.
2000: C M Varga; T J Wickham; Douglas A Lauffenburger
Receptor-mediated targeting of gene delivery vectors: insights from molecular mechanisms for improved vehicle design.
Biotechnology and bioengineering 2000;70(6):593-605.
2000: AR Asthagiri; C A Reinhart; Alan F Horwitz; Douglas A Lauffenburger
The role of transient ERK2 signals in fibronectin- and insulin-mediated DNA synthesis.
Journal of cell science 2000;113 Pt 24():4499-510.
2000: JM Haugh; Alan Wells; Douglas A Lauffenburger
Mathematical modeling of epidermal growth factor receptor signaling through the phospholipase C pathway: mechanistic insights and predictions for molecular interventions.
Biotechnology and bioengineering 2000;70(2):225-38.
2000: Peter W Zandstra; H V Le; George Q Daley; L G Griffith; Douglas A Lauffenburger
Leukemia inhibitory factor (LIF) concentration modulates embryonic stem cell self-renewal and differentiation independently of proliferation.
Biotechnology and bioengineering 2000;69(6):607-17.
2000: EM Fallon; Douglas A Lauffenburger
Computational model for effects of ligand/receptor binding properties on interleukin-2 trafficking dynamics and T cell proliferation response.
Biotechnology progress 2000;16(5):905-16.
2000: Douglas A Lauffenburger
Cell signaling pathways as control modules: complexity for simplicity?
Proceedings of the National Academy of Sciences of the United States of America 2000;97(10):5031-3.
2000: G Maheshwari; G Brown; Douglas A Lauffenburger; Alan Wells; Linda G Griffith
Cell adhesion and motility depend on nanoscale RGD clustering.
Journal of cell science 2000;113 ( Pt 10)():1677-86.
2000: EM Fallon; Stefano F Liparoto; K J Lee; Thomas L Ciardelli; Douglas A Lauffenburger
Increased endosomal sorting of ligand to recycling enhances potency of an interleukin-2 analog.
The Journal of biological chemistry 2000;275(10):6790-7.
2000: David V Schaffer; N A Fidelman; N Dan; Douglas A Lauffenburger
Vector unpacking as a potential barrier for receptor-mediated polyplex gene delivery.
Biotechnology and bioengineering 2000;67(5):598-606.
2000: Angela Glading; P Chang; Douglas A Lauffenburger; Alan Wells
Epidermal growth factor receptor activation of calpain is required for fibroblast motility and occurs via an ERK/MAP kinase signaling pathway.
The Journal of biological chemistry 2000;275(4):2390-8.
2000: Michael R Caplan; Peter N Moore; Shuguang Zhang; Roger Kamm; Douglas A Lauffenburger
Self-assembly of a beta-sheet protein governed by relief of electrostatic repulsion relative to van der Waals attraction.
Biomacromolecules 2000;1(4):627-31.
2000: AR Asthagiri; Douglas A Lauffenburger
Bioengineering models of cell signaling.
Annual review of biomedical engineering 2000;2():31-53.
1999: Alan Wells; M F Ware; F D Allen; Douglas A Lauffenburger
Shaping up for shipping out: PLCgamma signaling of morphology changes in EGF-stimulated fibroblast migration.
Cell motility and the cytoskeleton 1999;44(4):227-33.
1999: JM Haugh; A C Huang; H Steven Wiley; Alan Wells; Douglas A Lauffenburger
Internalized epidermal growth factor receptors participate in the activation of p21(ras) in fibroblasts.
The Journal of biological chemistry 1999;274(48):34350-60.
1999: AR Asthagiri; C M Nelson; Alan F Horwitz; Douglas A Lauffenburger
Quantitative relationship among integrin-ligand binding, adhesion, and signaling via focal adhesion kinase and extracellular signal-regulated kinase 2.
The Journal of biological chemistry 1999;274(38):27119-27.
1999: Shuguang Zhang; L Yan; Michael D Altman; M Lässle; Helen M Nugent; F Frankel; Douglas A Lauffenburger; George M Whitesides; A Rich
Biological surface engineering: a simple system for cell pattern formation.
Biomaterials 1999;20(13):1213-20.
1999: Jianying Dong; Lee K Opresko; Peter J Dempsey; Douglas A Lauffenburger; R J Coffey; H Steven Wiley
Metalloprotease-mediated ligand release regulates autocrine signaling through the epidermal growth factor receptor.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(11):6235-40.
1999: G Maheshwari; Alan Wells; Linda G Griffith; Douglas A Lauffenburger
Biophysical integration of effects of epidermal growth factor and fibronectin on fibroblast migration.
Biophysical journal 1999;76(5):2814-23.
1999: AR Asthagiri; Alan F Horwitz; Douglas A Lauffenburger
A rapid and sensitive quantitative kinase activity assay using a convenient 96-well format.
Analytical biochemistry 1999;269(2):342-7.
1999: JM Haugh; K Schooler; Alan Wells; H Steven Wiley; Douglas A Lauffenburger
Effect of epidermal growth factor receptor internalization on regulation of the phospholipase C-gamma1 signaling pathway.
The Journal of biological chemistry 1999;274(13):8958-65.
1999: SP Palecek; Alan F Horwitz; Douglas A Lauffenburger
Kinetic model for integrin-mediated adhesion release during cell migration.
Annals of biomedical engineering 1999;27(2):219-35.
1999: G Oehrtman; L Walker; B Will; Lee K Opresko; H Steven Wiley; Douglas A Lauffenburger
Quantitative assessment of autocrine cell loops.
Methods in molecular medicine 1999;18():143-54.
1998: Douglas A Lauffenburger; G T Oehrtman; L Walker; H Steven Wiley
Real-time quantitative measurement of autocrine ligand binding indicates that autocrine loops are spatially localized.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(26):15368-73.
1998: G Maheshwari; Douglas A Lauffenburger
Deconstructing (and reconstructing) cell migration.
Microscopy research and technique 1998;43(5):358-68.
1998: H Steven Wiley; Margaret F Woolf; Lee K Opresko; PM Burke; B Will; J R Morgan; Douglas A Lauffenburger
Removal of the membrane-anchoring domain of epidermal growth factor leads to intracrine signaling and disruption of mammary epithelial cell organization.
The Journal of cell biology 1998;143(5):1317-28.
1998: JM Haugh; Douglas A Lauffenburger
Analysis of receptor internalization as a mechanism for modulating signal transduction.
Journal of theoretical biology 1998;195(2):187-218.
1998: David V Schaffer; Douglas A Lauffenburger
Optimization of cell surface binding enhances efficiency and specificity of molecular conjugate gene delivery.
The Journal of biological chemistry 1998;273(43):28004-9.
1998: Douglas A Lauffenburger; EM Fallon; JM Haugh
Scratching the (cell) surface: cytokine engineering for improved ligand/receptor trafficking dynamics.
Chemistry & biology 1998;5(10):R257-63.
1998: M F Ware; Alan Wells; Douglas A Lauffenburger
Epidermal growth factor alters fibroblast migration speed and directional persistence reciprocally and in a matrix-dependent manner.
Journal of cell science 1998;111 ( Pt 16)():2423-32.
1998: C C Reddy; A Wells; Douglas A Lauffenburger
Comparative mitogenic potencies of EGF and TGF alpha and their dependence on receptor-limitation versus ligand-limitation.
Medical & biological engineering & computing 1998;36(4):499-507.
1998: SP Palecek; Anna Huttenlocher; Alan F Horwitz; Douglas A Lauffenburger
Physical and biochemical regulation of integrin release during rear detachment of migrating cells.
Journal of cell science 1998;111 ( Pt 7)():929-40.
1998: G T Oehrtman; H Steven Wiley; Douglas A Lauffenburger
Escape of autocrine ligands into extracellular medium: experimental test of theoretical model predictions.
Biotechnology and bioengineering 1998;57(5):571-82.
1998: E J Leon; N Verma; Shuguang Zhang; Douglas A Lauffenburger; Roger Kamm
Mechanical properties of a self-assembling oligopeptide matrix.
Journal of biomaterials science. Polymer edition 1998;9(3):297-312.
1997: Anna Huttenlocher; SP Palecek; Q Lu; W Zhang; R L Mellgren; Douglas A Lauffenburger; M H Ginsberg; Alan F Horwitz
Regulation of cell migration by the calcium-dependent protease calpain.
The Journal of biological chemistry 1997;272(52):32719-22.
1997: M F Ware; David A Tice; Sarah J Parsons; Douglas A Lauffenburger
Overexpression of cellular Src in fibroblasts enhances endocytic internalization of epidermal growth factor receptor.
The Journal of biological chemistry 1997;272(48):30185-90.
1997: A R French; Douglas A Lauffenburger
Controlling receptor/ligand trafficking: effects of cellular and molecular properties on endosomal sorting.
Annals of biomedical engineering 1997;25(4):690-707.
1997: S C Kuo; Daniel A Hammer; Douglas A Lauffenburger
Simulation of detachment of specifically bound particles from surfaces by shear flow.
Biophysical journal 1997;73(1):517-31.
1997: JM Haugh; Douglas A Lauffenburger
Physical modulation of intracellular signaling processes by locational regulation.
Biophysical journal 1997;72(5):2014-31.
1997: SP Palecek; J C Loftus; M H Ginsberg; Douglas A Lauffenburger; Alan F Horwitz
Integrin-ligand binding properties govern cell migration speed through cell-substratum adhesiveness.
Nature 1997;385(6616):537-40.
1996: C C Reddy; S K Niyogi; A Wells; H S Wiley; Douglas A Lauffenburger
Engineering epidermal growth factor for enhanced mitogenic potency.
Nature biotechnology 1996;14(13):1696-9.
1996: Douglas A Lauffenburger; L Chu; A French; G Oehrtman; C Reddy; A Wells; S Niyogi; H S Wiley
Engineering dynamics of growth factors and other therapeutic ligands.
Biotechnology and bioengineering 1996;52(1):61-80.
1996: A Saterbak; Douglas A Lauffenburger
Adhesion mediated by bonds in series.
Biotechnology progress 1996;12(5):682-99.
1996: A R French; Douglas A Lauffenburger
Intracellular receptor/ligand sorting based on endosomal retention components.
Biotechnology and bioengineering 1996;51(3):281-97.
1996: SP Palecek; C E Schmidt; Douglas A Lauffenburger; Alan F Horwitz
Integrin dynamics on the tail region of migrating fibroblasts.
Journal of cell science 1996;109 ( Pt 5)():941-52.
1996: C C Reddy; A Wells; Douglas A Lauffenburger
Receptor-mediated effects on ligand availability influence relative mitogenic potencies of epidermal growth factor and transforming growth factor alpha.
Journal of cellular physiology 1996;166(3):512-22.
1996: L Chu; H S Wiley; Douglas A Lauffenburger
Endocytic Relay as a Potential Means for Enhancing Ligand Transport through Cellular Tissue Matrices: Analysis and Possible Implications for Drug Delivery.
Tissue engineering 1996;2(1):17-38.
1995: C E Schmidt; J Dai; Douglas A Lauffenburger; M P Sheetz; Alan F Horwitz
Integrin-cytoskeletal interactions in neuronal growth cones.
The Journal of neuroscience : the official journal of the Society for Neuroscience 1995;15(5 Pt 1):3400-7.
1995: Douglas A Lauffenburger; K E Forsten; B Will; H S Wiley
Molecular/cell engineering approach to autocrine ligand control of cell function.
Annals of biomedical engineering 1995;23(3):208-15.
1995: A R French; D K Tadaki; S K Niyogi; Douglas A Lauffenburger
Intracellular trafficking of epidermal growth factor family ligands is directly influenced by the pH sensitivity of the receptor/ligand interaction.
The Journal of biological chemistry 1995;270(9):4334-40.
1995: B H Will; Douglas A Lauffenburger; H Steven Wiley
Studies on engineered autocrine systems: requirements for ligand release from cells producing an artificial growth factor.
Tissue engineering 1995;1(1):81-94.
1994: K E Forsten; Douglas A Lauffenburger
The role of low-affinity interleukin-2 receptors in autocrine ligand binding: alternative mechanisms for enhanced binding effect.
Molecular immunology 1994;31(10):739-51.
1994: C C Reddy; A Wells; Douglas A Lauffenburger
Proliferative response of fibroblasts expressing internalization-deficient epidermal growth factor (EGF) receptors is altered via differential EGF depletion effect.
Biotechnology progress 1994;10(4):377-84.
1994: C E Schmidt; T Chen; Douglas A Lauffenburger
Simulation of integrin-cytoskeletal interactions in migrating fibroblasts.
Biophysical journal 1994;67(1):461-74.
1994: A R French; G P Sudlow; H S Wiley; Douglas A Lauffenburger
Postendocytic trafficking of epidermal growth factor-receptor complexes is mediated through saturable and specific endosomal interactions.
The Journal of biological chemistry 1994;269(22):15749-55.
1994: J J Herbst; Lee K Opresko; B J Walsh; Douglas A Lauffenburger; H Steven Wiley
Regulation of postendocytic trafficking of the epidermal growth factor receptor through endosomal retention.
The Journal of biological chemistry 1994;269(17):12865-73.
1994: P Wu; J B Hoying; Stuart K Williams; B A Kozikowski; Douglas A Lauffenburger
Integrin-binding peptide in solution inhibits or enhances endothelial cell migration, predictably from cell adhesion.
Annals of biomedical engineering 1994;22(2):144-52.
1994: K E Forsten; Douglas A Lauffenburger
Probability of autocrine ligand capture by cell-surface receptors: implications for ligand secretion measurements.
Journal of computational biology : a journal of computational molecular cell biology 1994;1(1):15-23.
1993: S C Kuo; Douglas A Lauffenburger
Relationship between receptor/ligand binding affinity and adhesion strength.
Biophysical journal 1993;65(5):2191-200.
1993: C E Schmidt; Alan F Horwitz; Douglas A Lauffenburger; M P Sheetz
Integrin-cytoskeletal interactions in migrating fibroblasts are dynamic, asymmetric, and regulated.
The Journal of cell biology 1993;123(4):977-91.
1993: P A DiMilla; J A Stone; J A Quinn; Steven M Albelda; Douglas A Lauffenburger
Maximal migration of human smooth muscle cells on fibronectin and type IV collagen occurs at an intermediate attachment strength.
The Journal of cell biology 1993;122(3):729-37.
1993: A Saterbak; S C Kuo; Douglas A Lauffenburger
Heterogeneity and probabilistic binding contributions to receptor-mediated cell detachment kinetics.
Biophysical journal 1993;65(1):243-52.
1993: M Nunez; K H Mayo; C Starbuck; Douglas A Lauffenburger
pH sensitivity of epidermal growth factor receptor complexes.
Journal of cellular biochemistry 1993;51(3):312-21.
1992: K E Forsten; Douglas A Lauffenburger
Interrupting autocrine ligand-receptor binding: comparison between receptor blockers and ligand decoys.
Biophysical journal 1992;63(3):857-61.
1992: Roseanne M Ford; Douglas A Lauffenburger
A simple expression for quantifying bacterial chemotaxis using capillary assay data: application to the analysis of enhanced chemotactic responses from growth-limited cultures.
Mathematical biosciences 1992;109(2):127-49.
1992: C Starbuck; Douglas A Lauffenburger
Mathematical model for the effects of epidermal growth factor receptor trafficking dynamics on fibroblast proliferation responses.
Biotechnology progress 1992;8(2):132-43.
1992: K E Forsten; Douglas A Lauffenburger
Autocrine ligand binding to cell receptors. Mathematical analysis of competition by solution "decoys".
Biophysical journal 1992;61(2):518-29.
1991: M L Cano; Douglas A Lauffenburger; Sally H Zigmond
Kinetic analysis of F-actin depolymerization in polymorphonuclear leukocyte lysates indicates that chemoattractant stimulation increases actin filament number without altering the filament length distribution.
The Journal of cell biology 1991;115(3):677-87.
1991: C L Stokes; Douglas A Lauffenburger
Analysis of the roles of microvessel endothelial cell random motility and chemotaxis in angiogenesis.
Journal of theoretical biology 1991;152(3):377-403.
1991: P A DiMilla; K Barbee; Douglas A Lauffenburger
Mathematical model for the effects of adhesion and mechanics on cell migration speed.
Biophysical journal 1991;60(1):15-37.
1991: H Steven Wiley; J J Herbst; B J Walsh; Douglas A Lauffenburger; Michael G Rosenfeld; Gordon N Gill
The role of tyrosine kinase activity in endocytosis, compartmentation, and down-regulation of the epidermal growth factor receptor.
The Journal of biological chemistry 1991;266(17):11083-94.
1991: C L Stokes; Douglas A Lauffenburger; Stuart K Williams
Migration of individual microvessel endothelial cells: stochastic model and parameter measurement.
Journal of cell science 1991;99 ( Pt 2)():419-30.
1991: Roseanne M Ford; Douglas A Lauffenburger
Analysis of chemotactic bacterial distributions in population migration assays using a mathematical model applicable to steep or shallow attractant gradients.
Bulletin of mathematical biology 1991;53(5):721-49.
1991: S B Charnick; E S Fisher; Douglas A Lauffenburger
Computer simulations of cell-target encounter including biased cell motion toward targets: single and multiple cell-target simulations in two dimensions.
Bulletin of mathematical biology 1991;53(4):591-621.
1990: C L Stokes; M A Rupnick; Stuart K Williams; Douglas A Lauffenburger
Chemotaxis of human microvessel endothelial cells in response to acidic fibroblast growth factor.
Laboratory investigation; a journal of technical methods and pathology 1990;63(5):657-68.
1990: C Cozens-Roberts; J A Quinn; Douglas A Lauffenburger
Receptor-mediated cell attachment and detachment kinetics. II. Experimental model studies with the radial-flow detachment assay.
Biophysical journal 1990;58(4):857-72.
1990: C Cozens-Roberts; Douglas A Lauffenburger; J A Quinn
Receptor-mediated cell attachment and detachment kinetics. I. Probabilistic model and analysis.
Biophysical journal 1990;58(4):841-56.
1990: C L Stokes; P B Weisz; Stuart K Williams; Douglas A Lauffenburger
Inhibition of microvascular endothelial cell migration by beta-cyclodextrin tetradecasulfate and hydrocortisone.
Microvascular research 1990;40(2):279-84.
1990: S B Charnick; Douglas A Lauffenburger
Mathematical analysis of cell-target encounter rates in three dimensions. Effect of chemotaxis.
Biophysical journal 1990;57(5):1009-23.
1990: M R Wattenbarger; D J Graves; Douglas A Lauffenburger
Specific adhesion of glycophorin liposomes to a lectin surface in shear flow.
Biophysical journal 1990;57(4):765-77.
1990: E S Fisher; Douglas A Lauffenburger
Analysis of the effects of immune cell motility and chemotaxis on target elimination dynamics.
Mathematical biosciences 1990;98(1):73-102.
1990: B E Farrell; R P Daniele; Douglas A Lauffenburger
Quantitative relationships between single-cell and cell-population model parameters for chemosensory migration responses of alveolar macrophages to C5a.
Cell motility and the cytoskeleton 1990;16(4):279-93.
1989: K H Mayo; M Nunez; C Burke; C Starbuck; Douglas A Lauffenburger; C R Savage
Epidermal growth factor receptor binding is not a simple one-step process.
The Journal of biological chemistry 1989;264(30):17838-44.
1989: J E Glasgow; B E Farrell; E S Fisher; Douglas A Lauffenburger; R P Daniele
The motile response of alveolar macrophages. An experimental study using single-cell and cell population approaches.
The American review of respiratory disease 1989;139(2):320-9.
1988: M A Rupnick; C L Stokes; Stuart K Williams; Douglas A Lauffenburger
Quantitative analysis of random motility of human microvessel endothelial cells using a linear under-agarose assay.
Laboratory investigation; a journal of technical methods and pathology 1988;59(3):363-72.
1988: Jennifer J Linderman; Douglas A Lauffenburger
Analysis of intracellular receptor/ligand sorting in endosomes.
Journal of theoretical biology 1988;132(2):203-45.
1988: E S Fisher; Douglas A Lauffenburger; R P Daniele
The effect of alveolar macrophage chemotaxis on bacterial clearance from the lung surface.
The American review of respiratory disease 1988;137(5):1129-34.
1988: Robert T Tranquillo; Douglas A Lauffenburger; Sally H Zigmond
A stochastic model for leukocyte random motility and chemotaxis based on receptor binding fluctuations.
The Journal of cell biology 1988;106(2):303-9.
1988: Douglas A Lauffenburger; Robert T Tranquillo; Sally H Zigmond
Concentration gradients of chemotactic factors in chemotaxis assays.
Methods in enzymology 1988;162():85-101.
1988: Robert T Tranquillo; Sally H Zigmond; Douglas A Lauffenburger
Measurement of the chemotaxis coefficient for human neutrophils in the under-agarose migration assay.
Cell motility and the cytoskeleton 1988;11(1):1-15.
1987: Daniel A Hammer; Douglas A Lauffenburger
A dynamical model for receptor-mediated cell adhesion to surfaces.
Biophysical journal 1987;52(3):475-87.
1987: Douglas A Lauffenburger; M Rivero; F Kelly; R Ford; Joseph M DiRienzo
Bacterial chemotaxis. Cell flux model, parameter measurement, population dynamics, and genetic manipulation.
Annals of the New York Academy of Sciences 1987;506():281-95.
1987: Douglas A Lauffenburger; Jennifer J Linderman; L Berkowitz
Analysis of mammalian cell growth factor receptor dynamics.
Annals of the New York Academy of Sciences 1987;506():147-62.
1985: Douglas A Lauffenburger
Common ground.
Biotechnology progress 1985;1(4):d3.
1985: Douglas A Lauffenburger
Chemotaxis: analysis for quantitative studies.
Biotechnology progress 1985;1(3):151-60.
1985: Douglas A Lauffenburger
Stability of colicin plasmids in continuous culture: mathematical model and analysis.
Biotechnology progress 1985;1(1):53-9.
1981: Douglas A Lauffenburger; C R Kennedy
Analysis of a lumped model for tissue inflammation dynamics.
Mathematical biosciences 1981;53(3-4):189-221.