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HS Lu

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TOP 3
X Jiang; O Gurel; EA Mendiaz; G W Stearns; C L Clogston; HS Lu; Timothy D Osslund; Rashid Syed; KE Langley; Wayne A Hendrickson
Structure of the active core of human stem cell factor and analysis of binding to its receptor kit.
L A Merewether; J Le; MD Jones; R Lee; Grant Shimamoto; HS Lu
Development of disulfide peptide mapping and determination of disulfide structure of recombinant human osteoprotegerin chimera produced in Escherichia coli.
S Hara; V Katta; HS Lu
Peptide map procedure using immobilized protease cartridges in tandem for disulfide linkage identification of neu differentiation factor epidermal growth factor domain.

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All Publications

2000: X Jiang; O Gurel; EA Mendiaz; G W Stearns; C L Clogston; HS Lu; Timothy D Osslund; Rashid Syed; KE Langley; Wayne A Hendrickson
Structure of the active core of human stem cell factor and analysis of binding to its receptor kit.
The EMBO journal 2000;19(13):3192-203.
2000: L A Merewether; J Le; MD Jones; R Lee; Grant Shimamoto; HS Lu
Development of disulfide peptide mapping and determination of disulfide structure of recombinant human osteoprotegerin chimera produced in Escherichia coli.
Archives of biochemistry and biophysics 2000;375(1):101-10.
2000: S Hara; V Katta; HS Lu
Peptide map procedure using immobilized protease cartridges in tandem for disulfide linkage identification of neu differentiation factor epidermal growth factor domain.
Journal of chromatography. A 2000;867(1-2):151-60.
1999: YR Hsu; Rebecca Nybo; J K Sullivan; V Costigan; C S Spahr; C Wong; M Jones; A G Pentzer; JA Crouse; RE Pacifici; HS Lu; C Fred Morris; John Philo
Heparin is essential for a single keratinocyte growth factor molecule to bind and form a complex with two molecules of the extracellular domain of its receptor.
Biochemistry 1999;38(8):2523-34.
1999: HS Lu; P R Fausset; Linda O Narhi; TP Horan; K Shinagawa; Grant Shimamoto; Tom Boone
Chemical modification and site-directed mutagenesis of methionine residues in recombinant human granulocyte colony-stimulating factor: effect on stability and biological activity.
Archives of biochemistry and biophysics 1999;362(1):1-11.
1998: J L Liu; K V Lu; T Eris; V Katta; K R Westcott; Linda O Narhi; HS Lu
In vitro methionine oxidation of recombinant human leptin.
Pharmaceutical research 1998;15(4):632-40.
1998: YR Hsu; E W Hsu; V Katta; D Brankow; J Tseng; S Hu; C Fred Morris; W C Kenney; HS Lu
Human keratinocyte growth factor recombinantly expressed in Chinese hamster ovary cells: isolation of isoforms and characterization of post-translational modifications.
Protein expression and purification 1998;12(2):189-200.
1998: YR Hsu; W C Chang; EA Mendiaz; S Hara; D T Chow; M B Mann; KE Langley; HS Lu
Selective deamidation of recombinant human stem cell factor during in vitro aging: isolation and characterization of the aspartyl and isoaspartyl homodimers and heterodimers.
Biochemistry 1998;37(8):2251-62.
1998: MD Jones; S D Patterson; HS Lu
Determination of disulfide bonds in highly bridged disulfide-linked peptides by matrix-assisted laser desorption/ionization mass spectrometry with postsource decay.
Analytical chemistry 1998;70(1):136-43.
1997: MD Jones; J Hunt; J L Liu; S D Patterson; T Kohno; HS Lu
Determination of tumor necrosis factor binding protein disulfide structure: deviation of the fourth domain structure from the TNFR/NGFR family cysteine-rich region signature.
Biochemistry 1997;36(48):14914-23.
1997: YR Hsu; GM Wu; EA Mendiaz; Rashid Syed; Jette Wypych; R Toso; M B Mann; Tom Boone; Linda O Narhi; HS Lu; KE Langley
The majority of stem cell factor exists as monomer under physiological conditions. Implications for dimerization mediating biological activity.
The Journal of biological chemistry 1997;272(10):6406-15.
1996: S Hara; R Rosenfeld; HS Lu
Preventing the generation of artifacts during peptide map analysis of recombinant human insulin-like growth factor-I.
Analytical biochemistry 1996;243(1):74-9.
1996: HS Lu; YR Hsu; Linda O Narhi; S Karkare; F K Lin
Purification and characterization of human tissue prokallikrein and kallikrein isoforms expressed in Chinese hamster ovary cells.
Protein expression and purification 1996;8(2):227-37.
1996: HS Lu; YR Hsu; L I Lu; D Ruff; D Lyons; F K Lin
Isolation and characterization of human tissue kallikrein produced in Escherichia coli: biochemical comparison to the enzymatically inactive prokallikrein and methionyl kallikrein.
Protein expression and purification 1996;8(2):215-26.
1996: YR Hsu; Linda O Narhi; C Spahr; KE Langley; HS Lu
In vitro methionine oxidation of Escherichia coli-derived human stem cell factor: effects on the molecular structure, biological activity, and dimerization.
Protein science : a publication of the Protein Society 1996;5(6):1165-73.
1996: HS Lu; M D Jones; J H Shieh; EA Mendiaz; D Feng; P Watler; Linda O Narhi; KE Langley
Isolation and characterization of a disulfide-linked human stem cell factor dimer. Biochemical, biophysical, and biological comparison to the noncovalently held dimer.
The Journal of biological chemistry 1996;271(19):11309-16.
1996: M D Jones; Linda O Narhi; W C Chang; HS Lu
Refolding and oxidation of recombinant human stem cell factor produced in Escherichia coli.
The Journal of biological chemistry 1996;271(19):11301-8.
1996: A C Herman; Tom Boone; HS Lu
Characterization, formulation, and stability of Neupogen (Filgrastim), a recombinant human granulocyte-colony stimulating factor.
Pharmaceutical biotechnology 1996;9():303-28.
1995: K V Lu; MF Rohde; A R Thomason; W C Kenney; HS Lu
Mistranslation of a TGA termination codon as tryptophan in recombinant platelet-derived growth factor expressed in Escherichia coli.
The Biochemical journal 1995;309 ( Pt 2)():411-7.
1995: HS Lu; D Chang; D Brankow; D Wen
Detection of neu differentiation factor with a biospecific affinity sensor during chromatography.
Journal of chromatography. A 1995;705(1):163-9.
1995: HS Lu; D Chang; John Philo; K Zhang; Linda O Narhi; N Liu; M Zhang; J Sun; J Wen; D Yanagihara
Studies on the structure and function of glycosylated and nonglycosylated neu differentiation factors. Similarities and differences of the alpha and beta isoforms.
The Journal of biological chemistry 1995;270(9):4784-91.
1995: HS Lu; S Hara; L W Wong; M D Jones; V Katta; G Trail; A Zou; D Brankow; S Cole; S Hu
Post-translational processing of membrane-associated neu differentiation factor proisoforms expressed in mammalian cells.
The Journal of biological chemistry 1995;270(9):4775-83.
1995: HS Lu; W C Chang; EA Mendiaz; M B Mann; KE Langley; YR Hsu
Spontaneous dissociation-association of monomers of the human-stem-cell-factor dimer.
The Biochemical journal 1995;305 ( Pt 2)():563-8.
1995: Jette Wypych; L G Bennett; M G Schwartz; C L Clogston; HS Lu; Virginia C Broudy; T D Bartley; V P Parker; KE Langley
Soluble kit receptor in human serum.
Blood 1995;85(1):66-73.
1994: T D Bartley; J Bogenberger; P Hunt; Y S Li; HS Lu; F Martin; M S Chang; B Samal; Janet L Nichol; S Swift
Identification and cloning of a megakaryocyte growth and development factor that is a ligand for the cytokine receptor Mpl.
Cell 1994;77(7):1117-24.
1994: KE Langley; EA Mendiaz; N Liu; Linda O Narhi; L Zeni; C M Parseghian; C L Clogston; I Leslie; J A Pope; HS Lu
Properties of variant forms of human stem cell factor recombinantly expressed in Escherichia coli.
Archives of biochemistry and biophysics 1994;311(1):55-61.
1994: T D Bartley; R W Hunt; Andrew A Welcher; william boyle; V P Parker; R A Lindberg; HS Lu; A Colombero; R L Elliott; B A Guthrie
B61 is a ligand for the ECK receptor protein-tyrosine kinase.
Nature 1994;368(6471):558-60.
1994: E Watson; B Shah; L Leiderman; YR Hsu; S Karkare; HS Lu; F K Lin
Comparison of N-linked oligosaccharides of recombinant human tissue kallikrein produced by Chinese hamster ovary cells on microcarrier beads and in serum-free suspension culture.
Biotechnology progress 1994;10(1):39-44.
1994: M D Jones; L A Merewether; C L Clogston; HS Lu
Peptide map analysis of recombinant human granulocyte colony stimulating factor: elimination of methionine modification and nonspecific cleavages.
Analytical biochemistry 1994;216(1):135-46.
1993: HS Lu; P R Fausset; L S Sotos; C L Clogston; MF Rohde; K S Stoney; A C Herman
Isolation and characterization of three recombinant human granulocyte colony stimulating factor His-->Gln isoforms produced in Escherichia coli.
Protein expression and purification 1993;4(5):465-72.
1993: F K Lin; C H Lin; C C Chou; K Chen; HS Lu; W Bacheller; C Herrera; T Jones; Julie Chao; Lee Chao
Molecular cloning and sequence analysis of the monkey and human tissue kallikrein genes.
Biochimica et biophysica acta 1993;1173(3):325-8.
1993: C L Clogston; S Hu; Tom Boone; HS Lu
Glycosidase digestion, electrophoresis and chromatographic analysis of recombinant human granulocyte colony-stimulating factor glycoforms produced in Chinese hamster ovary cells.
Journal of chromatography 1993;637(1):55-62.
1992: HS Lu; C L Clogston; Jette Wypych; V P Parker; T D Lee; K Swiderek; R F Baltera; A C Patel; D C Chang; D W Brankow
Post-translational processing of membrane-associated recombinant human stem cell factor expressed in Chinese hamster ovary cells.
Archives of biochemistry and biophysics 1992;298(1):150-8.
1992: HS Lu; C L Clogston; Linda O Narhi; L A Merewether; W R Pearl; Tom Boone
Folding and oxidation of recombinant human granulocyte colony stimulating factor produced in Escherichia coli. Characterization of the disulfide-reduced intermediates and cysteine----serine analogs.
The Journal of biological chemistry 1992;267(13):8770-7.
1992: C L Clogston; YR Hsu; Tom Boone; HS Lu
Detection and quantitation of recombinant granulocyte colony-stimulating factor charge isoforms: comparative analysis by cationic-exchange chromatography, isoelectric focusing gel electrophoresis, and peptide mapping.
Analytical biochemistry 1992;202(2):375-83.
1991: Tsutomu Arakawa; D A Yphantis; J W Lary; Linda O Narhi; HS Lu; S J Prestrelski; C L Clogston; K M Zsebo; EA Mendiaz; Jette Wypych
Glycosylated and unglycosylated recombinant-derived human stem cell factors are dimeric and have extensive regular secondary structure.
The Journal of biological chemistry 1991;266(28):18942-8.
1991: K M Yen; M R Karl; L M Blatt; M J Simon; R B Winter; P R Fausset; HS Lu; A A Harcourt; K K Chen
Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
Journal of bacteriology 1991;173(17):5315-27.
1991: HS Lu; C L Clogston; Jette Wypych; P R Fausset; S Lauren; EA Mendiaz; K M Zsebo; KE Langley
Amino acid sequence and post-translational modification of stem cell factor isolated from buffalo rat liver cell-conditioned medium.
The Journal of biological chemistry 1991;266(13):8102-7.
1991: Yves A DeClerck; T D Yean; HS Lu; J Ting; KE Langley
Inhibition of autoproteolytic activation of interstitial procollagenase by recombinant metalloproteinase inhibitor MI/TIMP-2.
The Journal of biological chemistry 1991;266(6):3893-9.
1991: P R Fausset; HS Lu
Structural analysis of recombinant proteins prepared by semi-dry electroblotting after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Electrophoresis 1991;12(1):22-7.
1990: F H Martin; S V Suggs; KE Langley; HS Lu; J Ting; K H Okino; C Fred Morris; Ian K McNiece; F W Jacobsen; EA Mendiaz
Primary structure and functional expression of rat and human stem cell factor DNAs.
Cell 1990;63(1):203-11.
1990: K M Zsebo; Jette Wypych; Ian K McNiece; HS Lu; K A Smith; S B Karkare; R K Sachdev; V N Yuschenkoff; Neal Birkett; L R Williams
Identification, purification, and biological characterization of hematopoietic stem cell factor from buffalo rat liver--conditioned medium.
Cell 1990;63(1):195-201.
1990: M L Klein; T D Bartley; J M Davis; D W Whiteley; HS Lu
Isolation and structural characterization of three isoforms of recombinant consensus alpha interferon.
Archives of biochemistry and biophysics 1990;276(2):531-7.
1989: Y A De Clerck; T D Yean; B J Ratzkin; HS Lu; KE Langley
Purification and characterization of two related but distinct metalloproteinase inhibitors secreted by bovine aortic endothelial cells.
The Journal of biological chemistry 1989;264(29):17445-53.
1989: C L Clogston; Tom Boone; B C Crandall; EA Mendiaz; HS Lu
Disulfide structures of human interleukin-6 are similar to those of human granulocyte colony stimulating factor.
Archives of biochemistry and biophysics 1989;272(1):144-51.
1989: HS Lu; F K Lin; Lee Chao; Julie Chao
Human urinary kallikrein. Complete amino acid sequence and sites of glycosylation.
International journal of peptide and protein research 1989;33(4):237-49.
1989: HS Lu; Tom Boone; L M Souza; P H Lai
Disulfide and secondary structures of recombinant human granulocyte colony stimulating factor.
Archives of biochemistry and biophysics 1989;268(1):81-92.
1988: M L Klein; T D Bartley; P H Lai; HS Lu
Structural characterization of recombinant consensus interferon-alpha.
Journal of chromatography 1988;454():205-15.
1988: HS Lu; L B Tsai; W C Kenney; P H Lai
Identification of unusual replacement of methionine by norleucine in recombinant interleukin-2 produced by E. coli.
Biochemical and biophysical research communications 1988;156(2):807-13.
1988: L B Tsai; HS Lu; W C Kenney; C C Curless; M L Klein; P H Lai; D M Fenton; B W Altrock; M B Mann
Control of misincorporation of de novo synthesized norleucine into recombinant interleukin-2 in E. coli.
Biochemical and biophysical research communications 1988;156(2):733-9.
1988: HS Lu; M L Klein; P H Lai
Narrow-bore high-performance liquid chromatography of phenylthiocarbamyl amino acids and carboxypeptidase P digestion for protein C-terminal sequence analysis.
Journal of chromatography 1988;447(2):351-64.
1986: HS Lu; P H Lai
Use of narrow-bore high-performance liquid chromatography for microanalysis of protein structure.
Journal of chromatography 1986;368(2):215-31.
1986: K M Zsebo; HS Lu; J C Fieschko; L Goldstein; J Davis; K Duker; S V Suggs; P H Lai; G A Bitter
Protein secretion from Saccharomyces cerevisiae directed by the prepro-alpha-factor leader region.
The Journal of biological chemistry 1986;261(13):5858-65.