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Hugh Makin

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Sonoko Masuda; Martin Kaufmann; Valarie Byford; Mian Gao; René St-Arnaud; Alice Arabian; Hugh L J Makin; Joyce C Knutson; Stephen A Strugnell; Glenville Jones
Insights into Vitamin D metabolism using cyp24 over-expression and knockout systems in conjunction with liquid chromatography/mass spectrometry (LC/MS).
Neil J Schroeder; Jacky M Burrin; Kate Noonan; Hugh L J Makin; John Cunningham
Effects of 'non-calcaemic' vitamin D analogues on 24-hydroxylase expression in MG-63 osteoblast-like cells.
Valarie Byford; Stephen A Strugnell; Ruth Coldwell; Neil J Schroeder; Hugh L J Makin; Joyce C Knutson; Charles W Bishop; Glenville Jones
Use of vitamin D(4) analogs to investigate differences in hepatic and target cell metabolism of vitamins D(2) and D(3).

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2004: Sonoko Masuda; Martin Kaufmann; Valarie Byford; Mian Gao; René St-Arnaud; Alice Arabian; Hugh L J Makin; Joyce C Knutson; Stephen A Strugnell; Glenville Jones
Insights into Vitamin D metabolism using cyp24 over-expression and knockout systems in conjunction with liquid chromatography/mass spectrometry (LC/MS).
The Journal of steroid biochemistry and molecular biology 2004;89-90(1-5):149-53.
2003: Neil J Schroeder; Jacky M Burrin; Kate Noonan; Hugh L J Makin; John Cunningham
Effects of 'non-calcaemic' vitamin D analogues on 24-hydroxylase expression in MG-63 osteoblast-like cells.
Nephron. Physiology 2003;94(4):p62-73.
2002: Valarie Byford; Stephen A Strugnell; Ruth Coldwell; Neil J Schroeder; Hugh L J Makin; Joyce C Knutson; Charles W Bishop; Glenville Jones
Use of vitamin D(4) analogs to investigate differences in hepatic and target cell metabolism of vitamins D(2) and D(3).
Biochimica et biophysica acta 2002;1583(2):151-66.
2001: VN Shankar; Valarie Byford; David E Prosser; Neil J Schroeder; Hugh L J Makin; H Wiesinger; G Neef; Andreas Steinmeyer; Glenville Jones
Metabolism of a 20-methyl substituted series of vitamin D analogs by cultured human cells: apparent reduction of 23-hydroxylation of the side chain by the 20-methyl group.
Biochemical pharmacology 2001;61(7):893-902.
2001: VN Shankar; A E Propp; Neil J Schroeder; B W Surber; Hugh L J Makin; Glenville Jones
In vitro metabolism of 19-nor-1alpha, 25-(OH)2D2 in cultured cell lines: inducible synthesis of lipid- and water-soluble metabolites.
Archives of biochemistry and biophysics 2001;387(2):297-306.
2000: Stanley L-S Fan; Neil J Schroeder; Martin J Calverley; Jacky M Burrin; Hugh L J Makin; John Cunningham
Potent suppression of the parathyroid glands by hydroxylated metabolites of dihydrotachysterol(2).
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2000;15(12):1943-9.
2000: G C van den Bemd; FJ Dilworth; Hugh L J Makin; J M Prahl; Hector F Deluca; Glenville Jones; Huibert A P Pols; Johannes P T M van Leeuwen
Contribution of several metabolites of the vitamin D analog 20-epi-22-oxa-24a,26a,27a-tri-homo-1,25-(OH)(2) vitamin D(3) (KH 1060) to the overall biological activity of KH1060 by a shared mechanism of action.
Biochemical pharmacology 2000;59(6):621-7.
2000: Sonoko Masuda; Maya Kamao; Neil J Schroeder; Hugh L J Makin; Glenville Jones; Richard Kremer; Johng S Rhim; Toshio Okano
Characterization of 3-epi-1alpha,25-dihydroxyvitamin D3 involved in 1alpha,25-dihydroxyvitamin D3 metabolic pathway in cultured cell lines.
Biological & pharmaceutical bulletin 2000;23(2):133-9.
1999: Hugh L J Makin; DJ Trafford
Intoximeter 3000 and the four minute fallacy.
Medicine, science, and the law 1999;39(1):90-1.
1998: EB Mawer; Glenville Jones; Michael Davies; P E Still; Valarie Byford; Neil J Schroeder; Hugh L J Makin; Charles W Bishop; Joyce C Knutson
Unique 24-hydroxylated metabolites represent a significant pathway of metabolism of vitamin D2 in humans: 24-hydroxyvitamin D2 and 1,24-dihydroxyvitamin D2 detectable in human serum.
The Journal of clinical endocrinology and metabolism 1998;83(6):2156-66.
1997: FJ Dilworth; G R Williams; Anne-Marie Kissmeyer; Jeanet L Nielsen; Ernst Binderup; Martin J Calverley; Hugh L J Makin; Glenville Jones
The vitamin D analog, KH1060, is rapidly degraded both in vivo and in vitro via several pathways: principal metabolites generated retain significant biological activity.
Endocrinology 1997;138(12):5485-96.
1997: Anne-Marie Kissmeyer; Ernst Binderup; Lise Binderup; C Mørk Hansen; N R Andersen; Hugh L J Makin; Neil J Schroeder; VN Shankar; Glenville Jones
Metabolism of the vitamin D analog EB 1089: identification of in vivo and in vitro liver metabolites and their biological activities.
Biochemical pharmacology 1997;53(8):1087-97.
1997: VN Shankar; FJ Dilworth; Hugh L J Makin; Neil J Schroeder; DJ Trafford; Anne-Marie Kissmeyer; Martin J Calverley; Ernst Binderup; Glenville Jones
Metabolism of the vitamin D analog EB1089 by cultured human cells: redirection of hydroxylation site to distal carbons of the side-chain.
Biochemical pharmacology 1997;53(6):783-93.
1997: T K Kwan; M A Kraevskaya; Hugh L J Makin; DJ Trafford; DB Gower
Use of gas chromatographic-mass spectrometric techniques in studies of androst-16-ene and androgen biosynthesis in human testis; cytosolic specific binding of 5alpha-androst-16-en-3-one.
The Journal of steroid biochemistry and molecular biology 1997;60(1-2):137-46.
1996: Glenville Jones; V Byford; Hugh L J Makin; R Kremer; R H Rice; L A deGraffenried; Joyce C Knutson; Charles W Bishop
Anti-proliferative activity and target cell catabolism of the vitamin D analog 1 alpha,24(S)-(OH)2D2 in normal and immortalized human epidermal cells.
Biochemical pharmacology 1996;52(1):133-40.
1996: S Masuda; V Byford; R Kremer; Hugh L J Makin; N Kubodera; Y Nishii; A Okazaki; T Okano; T Kobayashi; Glenville Jones
In vitro metabolism of the vitamin D analog, 22-oxacalcitriol, using cultured osteosarcoma, hepatoma, and keratinocyte cell lines.
The Journal of biological chemistry 1996;271(15):8700-8.
1995: S Strugnell; V Byford; Hugh L J Makin; Robert M Moriarty; R Gilardi; L W LeVan; Joyce C Knutson; Charles W Bishop; Glenville Jones
1 alpha,24(S)-dihydroxyvitamin D2: a biologically active product of 1 alpha-hydroxyvitamin D2 made in the human hepatoma, Hep3B.
The Biochemical journal 1995;310 ( Pt 1)():233-41.
1995: FJ Dilworth; I Scott; A Green; S Strugnell; Y D Guo; E A Roberts; R Kremer; Martin J Calverley; Hugh L J Makin; Glenville Jones
Different mechanisms of hydroxylation site selection by liver and kidney cytochrome P450 species (CYP27 and CYP24) involved in vitamin D metabolism.
The Journal of biological chemistry 1995;270(28):16766-74.
1995: E C Castenskiold; Stephen M Kelsey; P W Collins; R D Coldwell; P D Allen; L E Side; Hugh L J Makin; A H Goldstone; Adrian C Newland
Functional hyperactivity of monocytes after bone marrow transplantation: possible relevance for the development of post-transplant complications or relapse.
Bone marrow transplantation 1995;15(6):879-84.
1994: D J Trafford; Hugh L J Makin
Breath-alcohol concentration may not always reflect the concentration of alcohol in blood.
Journal of analytical toxicology 1994;18(4):225-8.
1994: Neil J Schroeder; D J Trafford; J Cunningham; Glenville Jones; Hugh L J Makin
In vivo dihydrotachysterol2 metabolism in normal man: 1 alpha- and 1 beta-hydroxylation of 25-hydroxydihydrotachysterol2 and effects on plasma parathyroid hormone and 1 alpha,25-dihydroxyvitamin D3 concentrations.
The Journal of clinical endocrinology and metabolism 1994;78(6):1481-7.
1994: FJ Dilworth; Martin J Calverley; Hugh L J Makin; Glenville Jones
Increased biological activity of 20-epi-1,25-dihydroxyvitamin D3 is due to reduced catabolism and altered protein binding.
Biochemical pharmacology 1994;47(6):987-93.
1994: S Masuda; S Strugnell; Martin J Calverley; Hugh L J Makin; R Kremer; Glenville Jones
In vitro metabolism of the anti-psoriatic vitamin D analog, calcipotriol, in two cultured human keratinocyte models.
The Journal of biological chemistry 1994;269(7):4794-803.
1994: Stephen M Kelsey; R D Coldwell; Hugh L J Makin; Denise Syndercombe-Court; Adrian C Newland
Neopterin release by myeloid leukaemic cells can be synergistically augmented by 1,25-dihydroxyvitamin D3 in combination with gamma interferon or granulocyte-macrophage colony stimulating factor.
The Journal of steroid biochemistry and molecular biology 1994;48(1):95-9.
1993: Peter H Jellinck; Hugh L J Makin; D W Sepkovic; H Leon Bradlow
Influence of indole carbinols and growth hormone on the metabolism of 4-androstenedione by rat liver microsomes.
The Journal of steroid biochemistry and molecular biology 1993;46(6):791-8.
1993: F Qaw; Martin J Calverley; Neil J Schroeder; D J Trafford; Hugh L J Makin; Glenville Jones
In vivo metabolism of the vitamin D analog, dihydrotachysterol. Evidence for formation of 1 alpha,25- and 1 beta,25-dihydroxy-dihydrotachysterol metabolites and studies of their biological activity.
The Journal of biological chemistry 1993;268(1):282-92.
1992: T K Kwan; M A Kraevskaya; D J Trafford; Hugh L J Makin; D B Gower
Studies of 16-androstene steroid biosynthesis and binding in human testis.
Biochemical Society transactions 1992;20(4):368S.
1992: T K Kwan; D J Trafford; Hugh L J Makin; A I Mallet; D B Gower
GC-MS studies of 16-androstenes and other C19 steroids in human semen.
The Journal of steroid biochemistry and molecular biology 1992;43(6):549-56.
1992: R D Coldwell; J Nolan; D J Trafford; E B Mawer; Hugh L J Makin
The use of octadecyl-bonded microparticulate silica in the separation of free and bound fractions during saturation analysis of vitamin D metabolites.
Journal of pharmaceutical and biomedical analysis 1992;10(9):631-7.
1992: Stephen M Kelsey; Adrian C Newland; J Cunningham; Hugh L J Makin; R D Coldwell; M J Mills; I R Grant
Sustained haematological response to high-dose oral alfacalcidol in patients with myelodysplastic syndromes.
Lancet 1992;340(8814):316-7.
1992: Neil J Schroeder; F Qaw; Martin J Calverley; D J Trafford; Glenville Jones; Hugh L J Makin
Polar metabolites of dihydrotachysterol3 in the rat. Comparison with in vitro metabolites of 1 alpha,25-dihydroxydihydrotachysterol3.
Biochemical pharmacology 1992;43(9):1893-905.
1992: F S Qaw; Hugh L J Makin; Glenville Jones
Metabolism of 25-hydroxydihydrotachysterol3 in bone cells in vitro.
Steroids 1992;57(5):236-43.
1992: F Qaw; Neil J Schroeder; Martin J Calverley; D J Trafford; Hugh L J Makin; Glenville Jones
The metabolism of dihydrotachysterols: renal side chain and non-renal nuclear hydroxylations in vivo and in vitro.
The Journal of steroid biochemistry and molecular biology 1992;41(3-8):859-70.
1992: J Cunningham; J Beer; R D Coldwell; K Noonan; N Sawyer; Hugh L J Makin
Dialysate calcium reduction in CAPD patients treated with calcium carbonate and alfacalcidol.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 1992;7(1):63-8.
1991: D J Trafford; R D Coldwell; Hugh L J Makin
Gas chromatography-mass spectrometry in the investigation of on-column dehydration of steroid hormones during gas-liquid chromatography.
Journal of pharmaceutical and biomedical analysis 1991;9(10-12):1095-105.
1990: R D Coldwell; D J Trafford; M J Varley; D N Kirk; Hugh L J Makin
Stable isotope-labeled vitamin D, metabolites and chemical analogs: synthesis and use in mass spectrometric studies.
Steroids 1990;55(10):418-32.
1990: J Cunningham; R D Coldwell; G Jones; H S Tenenhouse; D J Trafford; Hugh L J Makin
Plasma 24,25-dihydroxyvitamin D3 concentrations in X-linked hypophosphatemic mice: studies using mass fragmentographic and radioreceptor assays.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 1990;5(2):173-7.
1989: J M Street; D J Trafford; Hugh L J Makin
Carrier gas flow-rate and injection system in capillary gas chromatography of unconjugated and glycine-conjugated bile acids.
Journal of chromatography 1989;495():31-40.
1989: R A Cattini; Hugh L J Makin; D J Trafford; P Vanezis
An apparent fatal overdose of terodiline.
Journal of analytical toxicology 1989;13(2):110-2.
1989: R D Coldwell; D J Trafford; M J Varley; D N Kirk; Hugh L J Makin
Measurement of 25-hydroxyvitamin D2, 25-hydroxyvitamin D3, 24,25-dihydroxyvitamin D2 and 25,26-dihydroxyvitamin D2 in a single plasma sample by mass fragmentography.
Clinica chimica acta; international journal of clinical chemistry 1989;180(2):157-68.
1988: C Porteous; D J Trafford; Hugh L J Makin; J Cunningham; Glenville Jones
Use of mass spectrometry in the identification of in vivo and in vitro metabolites of dihydrotachysterol3 in the rat.
Biomedical & environmental mass spectrometry 1988;16(1-12):87-92.
1988: R D Coldwell; D J Trafford; M J Varley; Hugh L J Makin; D N Kirk
The measurement of vitamins D2 and D3 and seven major metabolites in a single sample of human plasma using gas chromatography/mass spectrometry.
Biomedical & environmental mass spectrometry 1988;16(1-12):81-5.
1988: Glenville Jones; N Edwards; D Vriezen; C Porteous; D J Trafford; J Cunningham; Hugh L J Makin
Isolation and identification of seven metabolites of 25-hydroxydihydrotachysterol3 formed in the isolated perfused rat kidney: a model for the study of side-chain metabolism of vitamin D.
Biochemistry 1988;27(18):7070-9.
1987: C E Porteous; R D Coldwell; D J Trafford; Hugh L J Makin
Recent developments in the measurement of vitamin D and its metabolites in human body fluids.
Journal of steroid biochemistry 1987;28(6):785-801.
1987: R D Coldwell; C E Porteous; D J Trafford; Hugh L J Makin
Gas chromatography-mass spectrometry and the measurement of vitamin D metabolites in human serum or plasma.
Steroids 1987;49(1-3):155-96.
1985: N D Martin; G J Snodgrass; R D Cohen; C E Porteous; R D Coldwell; D J Trafford; Hugh L J Makin
Vitamin D metabolites in idiopathic infantile hypercalcaemia.
Archives of disease in childhood 1985;60(12):1140-3.
1985: R D Coldwell; D J Trafford; Hugh L J Makin
Improved chromatographic determination of 25-hydroxyvitamins D2 and D3.
Clinical chemistry 1985;31(11):1919-20.
1985: J M Street; D J Trafford; Hugh L J Makin
Extraction and fractionation of bile acids and their conjugates using pre-packed microparticulate silica cartridges (Sep-Pak Sil and Bond-Elut C18).
Journal of chromatography 1985;343(2):259-70.
1985: R D Coldwell; D J Trafford; Hugh L J Makin; M J Varley
Specific mass fragmentographic assay for 25,26-dihydroxyvitamin D in human plasma using a deuterated internal standard.
Journal of chromatography 1985;338(2):289-302.
1984: R D Coldwell; D J Trafford; Hugh L J Makin; M J Varley; D N Kirk
Specific estimation of 24,25-dihydroxyvitamin D in plasma by gas chromatography-mass spectrometry.
Clinical chemistry 1984;30(7):1193-8.
1983: Hugh L J Makin; D A Seamark; D J Trafford
Vitamin D and its metabolites in human breast milk.
Archives of disease in childhood 1983;58(9):750-3.
1983: C J Rhodes; P A Claridge; D J Trafford; Hugh L J Makin
An evaluation of the use of Sep-Pak C18 cartridges for the extraction of vitamin D3 and some of its metabolites from plasma and urine.
Journal of steroid biochemistry 1983;19(3):1349-54.
1983: D J Trafford; Hugh L J Makin
Studies on the presence of 25-hydroxyvitamin D in human saliva.
Clinica chimica acta; international journal of clinical chemistry 1983;129(1):19-25.
1982: H Turnbull; D J Trafford; Hugh L J Makin
A rapid and simple method for the measurement of plasma 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 using Sep-Pak C18 cartridges and a single high-performance liquid chromatographic step.
Clinica chimica acta; international journal of clinical chemistry 1982;120(1):65-76.
1981: D J Trafford; D A Seamark; H Turnbull; Hugh L J Makin
High-performance liquid chromatography of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in human plasma. Use of isotachysterols and a comparison with gas chromatography--mass spectrometry.
Journal of chromatography 1981;226(2):351-60.
1981: J M Holly; D J Trafford; J W Sear; Hugh L J Makin
The in vivo metabolism of Althesin (alphaxalone + alphadolone acetate) in man.
The Journal of pharmacy and pharmacology 1981;33(7):427-33.
1981: J M Lillington; D J Trafford; Hugh L J Makin
A rapid and simple method for the esterification of fatty acids and steroid carboxylic acids prior to gas-liquid chromatography.
Clinica chimica acta; international journal of clinical chemistry 1981;111(1):91-8.
1981: D A Seamark; D J Trafford; Hugh L J Makin
The estimation of vitamin D and its metabolites in human plasma.
Journal of steroid biochemistry 1981;14(1):111-23.
1980: D A Seamark; D J Trafford; P G Hiscocks; Hugh L J Makin
High-performance liquid chromatography of vitamin D: enhanced ultraviolet absorbance by prior conversion to isotachysterol derivatives.
Journal of chromatography 1980;197(2):271-3.
1980: D A Seamark; D J Trafford; Hugh L J Makin
The estimation of vitamin D and some metabolites in human plasma by mass fragmentography.
Clinica chimica acta; international journal of clinical chemistry 1980;106(1):51-62.
1980: D A Seamark; D J Trafford; Hugh L J Makin
A new procedure for the formation of isotachysterol derivatives of subnanomole quantities of ergocalciferol, cholecalciferol and its metabolites prior to gas liquid chromatography.
Journal of steroid biochemistry 1980;13(9):1057-63.
1980: J W Sear; J M Holly; D J Trafford; Hugh L J Makin
Plasma concentrations of alphaxalone by gas chromatography: comparison with other gas chromatographic methods and gas chromatography-mass spectrometry.
The Journal of pharmacy and pharmacology 1980;32(5):349-52.