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Graeme Milligan

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Suparna Patowary; Tian-Rui Xu; Elisa Alvarez-Curto; Jessica D Holz; Graeme Milligan; Julie A Oliver; Valerica Raicu
The muscarinic M3 acetylcholine receptor exists as two differently sized complexes at the plasma membrane.
Zaynab Neetoo-Isseljee; Craig Southern; Debra L Taylor; Nicholas Harries; Jeff Jerman; Amanda E MacKenzie; Edward G McIver; Graeme Milligan
High-throughput identification and characterization of novel, species-selective GPR35 agonists.
Brian D Hudson; Trond Ulven; Graeme Milligan
The therapeutic potential of allosteric ligands for free fatty acid sensitive GPCRs.

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2013: Suparna Patowary; Tian-Rui Xu; Elisa Alvarez-Curto; Jessica D Holz; Graeme Milligan; Julie A Oliver; Valerica Raicu
The muscarinic M3 acetylcholine receptor exists as two differently sized complexes at the plasma membrane.
The Biochemical journal 2013;452(2):303-12.
2013: Zaynab Neetoo-Isseljee; Craig Southern; Debra L Taylor; Nicholas Harries; Jeff Jerman; Amanda E MacKenzie; Edward G McIver; Graeme Milligan
High-throughput identification and characterization of novel, species-selective GPR35 agonists.
The Journal of pharmacology and experimental therapeutics 2013;344(3):568-78.
2013: Brian D Hudson; Trond Ulven; Graeme Milligan
The therapeutic potential of allosteric ligands for free fatty acid sensitive GPCRs.
Current topics in medicinal chemistry 2013;13(1):14-25.
2013: Richard J Ward; Tian-Rui Xu; Graeme Milligan
GPCR oligomerization and receptor trafficking.
Methods in enzymology 2013;521():69-90.
2013: Carolyn Clarke; Monica Flores-Muñoz; Clare A McKinney; Graeme Milligan; Stuart A Nicklin
Regulation of cardiovascular remodeling by the counter-regulatory axis of the renin-angiotensin system.
Future cardiology 2013;9(1):23-38.
2012: Laura Jenkins; Jennifer E Lappin; Amanda E MacKenzie; Edward G McIver; Zaynab Neetoo-Isseljee; Stuart A Nicklin; Craig Southern; Debra L Taylor; Nicholas Harries; Graeme Milligan
Antagonists of GPR35 display high species ortholog selectivity and varying modes of action.
The Journal of pharmacology and experimental therapeutics 2012;343(3):683-95.
2012: Brian D Hudson; Evi Kostenis; David R Adams; Elisabeth Christiansen; Manuel Grundmann; Irina G Tikhonova; Trond Ulven; Graeme Milligan
Chemically engineering ligand selectivity at the free fatty acid receptor 2 based on pharmacological variation between species orthologs.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012;26(12):4951-65.
2012: Brian D Hudson; Sunil K Pandey; Irina G Tikhonova; Trond Ulven; Graeme Milligan
Extracellular ionic locks determine variation in constitutive activity and ligand potency between species orthologs of the free fatty acid receptors FFA2 and FFA3.
The Journal of biological chemistry 2012;287(49):41195-209.
2012: Tian-Rui Xu; John D Pediani; Richard J Ward; Graeme Milligan
Intramolecular fluorescence resonance energy transfer (FRET) sensors of the orexin OX1 and OX2 receptors identify slow kinetics of agonist activation.
The Journal of biological chemistry 2012;287(18):14937-49.
2012: Katie White; Nicole M Kane; Graeme Milligan; Andrew H Baker
The role of miRNA in stem cell pluripotency and commitment to the vascular endothelial lineage.
Microcirculation (New York, N.Y. : 1994) 2012;19(3):196-207.
2012: Nicole M Kane; Ruifang Lu; Ruth M Mackenzie; Angela McCahill; John McClure; Marco Meloni; Graeme Milligan; joanne mountford; Christopher Breen; Christian Delles; Betty Descamps; Costanza Emanueli; Lynsey Howard; Andrew H Baker
Role of microRNAs 99b, 181a, and 181b in the differentiation of human embryonic stem cells to vascular endothelial cells.
Stem cells (Dayton, Ohio) 2012;30(4):643-54.
2012: Chantevy Pou; Leigh A Stoddart; Clotilde Mannoury La Cour; Mark J Millan; Graeme Milligan
Functional homomers and heteromers of dopamine D2L and D3 receptors co-exist at the cell surface.
The Journal of biological chemistry 2012;287(12):8864-78.
2012: Selim Terhzaz; Pablo Cabrero; Julian A T Dow; Brian D Hudson; Graeme Milligan; Jonathan C Radford; Joris H Robben; Shireen A Davies
Mechanism and function of Drosophila capa GPCR: a desiccation stress-responsive receptor with functional homology to human neuromedinU receptor.
PloS one 2012;7(1):e29897.
2011: Elisa Alvarez-Curto; Carsten Hoffmann; Martin J Lohse; John D Pediani; Rudi Prihandoko; Christofer S Tautermann; Andrew B Tobin; Jurriaan M Zwier; Graeme Milligan
Developing chemical genetic approaches to explore G protein-coupled receptor function: validation of the use of a receptor activated solely by synthetic ligand (RASSL).
Molecular pharmacology 2011;80(6):1033-46.
2011: Richard J Ward; John D Pediani; Graeme Milligan
Heteromultimerization of cannabinoid CB(1) receptor and orexin OX(1) receptor generates a unique complex in which both protomers are regulated by orexin A.
The Journal of biological chemistry 2011;286(43):37414-28.
2011: Lynsey Howard; Nicole M Kane; Graeme Milligan; Andrew H Baker
MicroRNAs regulating cell pluripotency and vascular differentiation.
Vascular pharmacology 2011;55(4):69-78.
2011: Tian-Rui Xu; John D Pediani; Richard J Ward; Graeme Milligan
The orexin OX(1) receptor exists predominantly as a homodimer in the basal state: potential regulation of receptor organization by both agonist and antagonist ligands.
The Biochemical journal 2011;439(1):171-83.
2011: Marcel Van Lith; Graeme Milligan; John Pediani; Shweta Tiwari; Neil J Bulleid
Real-time monitoring of redox changes in the mammalian endoplasmic reticulum.
Journal of cell science 2011;124(Pt 14):2349-56.
2011: Nicola J Smith; Leigh A Stoddart; Irina G Tikhonova; Christian Tränkle; Trond Ulven; Richard J Ward; David R Adams; Brian D Hudson; Evi Kostenis; Joanne C Morris; Graeme Milligan
Extracellular loop 2 of the free fatty acid receptor 2 mediates allosterism of a phenylacetamide ago-allosteric modulator.
Molecular pharmacology 2011;80(1):163-73.
2011: Graeme Milligan
Orthologue selectivity and ligand bias: translating the pharmacology of GPR35.
Trends in pharmacological sciences 2011;32(5):317-25.
2011: Richard J Ward; John D Pediani; Graeme Milligan
Ligand-induced internalization of the orexin OX(1) and cannabinoid CB(1) receptors assessed via N-terminal SNAP and CLIP-tagging.
British journal of pharmacology 2011;162(6):1439-52.
2011: D F Anthony; Y Y Sin; S Vadrevu; N Advant; J P Day; A M Byrne; M J Lynch; Graeme Milligan; Miles D Houslay; George S Baillie
β-Arrestin 1 inhibits the GTPase-activating protein function of ARHGAP21, promoting activation of RhoA following angiotensin II type 1A receptor stimulation.
Molecular and cellular biology 2011;31(5):1066-75.
2011: Laura Jenkins; Elisa Alvarez-Curto; Kate Campbell; Sabrina de Munnik; Meritxell Canals; Sabine Schlyer; Graeme Milligan
Agonist activation of the G protein-coupled receptor GPR35 involves transmembrane domain III and is transduced via Gα₁₃ and β-arrestin-2.
British journal of pharmacology 2011;162(3):733-48.
2011: Nicola J Smith; Kirstie A Bennett; Graeme Milligan
When simple agonism is not enough: emerging modalities of GPCR ligands.
Molecular and cellular endocrinology 2011;331(2):241-7.
2011: Richard J Ward; Elisa Alvarez-Curto; Graeme Milligan
Using the Flp-In™ T-Rex™ system to regulate GPCR expression.
Methods in molecular biology (Clifton, N.J.) 2011;746():21-37.
2010: Peter Burton; David R Adams; Achamma Abraham; Robert W Allcock; Zhong Jiang; Angela McCahill; Jane Gilmour; John McAbney; Alexandra Kaupisch; Nicole Kane; George S Baillie; Andrew H Baker; Graeme Milligan; Miles D Houslay; joanne mountford
Erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA) blocks differentiation and maintains the expression of pluripotency markers in human embryonic stem cells.
The Biochemical journal 2010;432(3):575-84.
2010: Laura Jenkins; Jose Brea; Nicola J Smith; Brian D Hudson; Graeme Reilly; Nia J Bryant; Marian Castro; María-Isabel Loza; Graeme Milligan
Identification of novel species-selective agonists of the G-protein-coupled receptor GPR35 that promote recruitment of β-arrestin-2 and activate Gα13.
The Biochemical journal 2010;432(3):451-9.
2010: Nicola J Smith; Graeme Milligan
Allostery at G protein-coupled receptor homo- and heteromers: uncharted pharmacological landscapes.
Pharmacological reviews 2010;62(4):701-25.
2010: Nicole Kane; Ali Nowrouzi; Sayandip Mukherjee; Michael P Blundell; Jenny A Greig; Wai Kwong Lee; Miles D Houslay; Graeme Milligan; joanne mountford; Christof von Kalle; Manfred Schmidt; Adrian J Thrasher; Andrew H Baker
Lentivirus-mediated reprogramming of somatic cells in the absence of transgenic transcription factors.
Molecular therapy : the journal of the American Society of Gene Therapy 2010;18(12):2139-45.
2010: Juan F Lopez-Gimenez; Graeme Milligan
Opioid regulation of mu receptor internalisation: relevance to the development of tolerance and dependence.
CNS & neurological disorders drug targets 2010;9(5):616-26.
2010: Elisa Alvarez-Curto; John D Pediani; Graeme Milligan
Applications of fluorescence and bioluminescence resonance energy transfer to drug discovery at G protein coupled receptors.
Analytical and bioanalytical chemistry 2010;398(1):167-80.
2010: Peter Burton; David R Adams; Achamma Abraham; Robert W Allcock; Zhong Jiang; Angela McCahill; Jane Gilmour; John McAbney; Nicole Kane; George S Baillie; Fergus R McKenzie; Andrew H Baker; Miles D Houslay; joanne mountford; Graeme Milligan
Identification and characterization of small-molecule ligands that maintain pluripotency of human embryonic stem cells.
Biochemical Society transactions 2010;38(4):1058-61.
2010: Elisa Alvarez-Curto; Richard J Ward; John D Pediani; Graeme Milligan
Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogeneous time-resolved FRET.
The Journal of biological chemistry 2010;285(30):23318-30.
2010: Nicole Kane; Marco Meloni; Helen L Spencer; Margaret A Craig; Raimund Strehl; Graeme Milligan; Miles D Houslay; joanne mountford; Costanza Emanueli; Andrew H Baker
Derivation of endothelial cells from human embryonic stem cells by directed differentiation: analysis of microRNA and angiogenesis in vitro and in vivo.
Arteriosclerosis, thrombosis, and vascular biology 2010;30(7):1389-97.
2010: Laura Saenz Del Burgo; Graeme Milligan
Heterodimerisation of G protein-coupled receptors: implications for drug design and ligand screening.
Expert opinion on drug discovery 2010;5(5):461-74.
2010: Graeme Milligan
The role of dimerisation in the cellular trafficking of G-protein-coupled receptors.
Current opinion in pharmacology 2010;10(1):23-9.
2010: Tian-Rui Xu; Vladislav Vyshemirsky; Amélie Gormand; Alex von Kriegsheim; Mark Girolami; George S Baillie; Dominic Ketley; Allan J Dunlop; Graeme Milligan; Miles D Houslay; Walter Kolch
Inferring signaling pathway topologies from multiple perturbation measurements of specific biochemical species.
Science signaling 2010;3(113):ra20.
2009: Kirstie A Bennett; Christopher J Langmead; Alan Wise; Graeme Milligan
Growth hormone secretagogues and growth hormone releasing peptides act as orthosteric super-agonists but not allosteric regulators for activation of the G protein Galpha(o1) by the Ghrelin receptor.
Molecular pharmacology 2009;76(4):802-11.
2009: Graeme Milligan; Leigh Stoddart; Nicola J Smith
Agonism and allosterism: the pharmacology of the free fatty acid receptors FFA2 and FFA3.
British journal of pharmacology 2009;158(1):146-53.
2009: Graeme Milligan; J C McGrath
GPCR theme editorial.
British journal of pharmacology 2009;158(1):1-4.
2009: Graeme Milligan
G protein-coupled receptor hetero-dimerization: contribution to pharmacology and function.
British journal of pharmacology 2009;158(1):5-14.
2009: Nicola J Smith; Leigh Stoddart; Nicola M Devine; Laura Jenkins; Graeme Milligan
The action and mode of binding of thiazolidinedione ligands at free fatty acid receptor 1.
The Journal of biological chemistry 2009;284(26):17527-39.
2009: Richard J Ward; Laura Jenkins; Graeme Milligan
Selectivity and functional consequences of interactions of family A G protein-coupled receptors with neurochondrin and periplakin.
Journal of neurochemistry 2009;109(1):182-92.
2009: Shirley Appelbe; Graeme Milligan
Chapter 10. Hetero-oligomerization of chemokine receptors.
Methods in enzymology 2009;461():207-25.
2009: Meritxell Canals; Juan F Lopez-Gimenez; Graeme Milligan
Cell surface delivery and structural re-organization by pharmacological chaperones of an oligomerization-defective alpha(1b)-adrenoceptor mutant demonstrates membrane targeting of GPCR oligomers.
The Biochemical journal 2009;417(1):161-72.
2008: Leigh Stoddart; Nicola J Smith; Graeme Milligan
International Union of Pharmacology. LXXI. Free fatty acid receptors FFA1, -2, and -3: pharmacology and pathophysiological functions.
Pharmacological reviews 2008;60(4):405-17.
2008: Leigh Stoddart; Nicola J Smith; Laura Jenkins; Andrew J Brown; Graeme Milligan
Conserved polar residues in transmembrane domains V, VI, and VII of free fatty acid receptor 2 and free fatty acid receptor 3 are required for the binding and function of short chain fatty acids.
The Journal of biological chemistry 2008;283(47):32913-24.
2008: Juan F Lopez-Gimenez; M Teresa Vilaró; Graeme Milligan
Morphine desensitization, internalization, and down-regulation of the mu opioid receptor is facilitated by serotonin 5-hydroxytryptamine2A receptor coactivation.
Molecular pharmacology 2008;74(5):1278-91.
2008: Tian-Rui Xu; George S Baillie; Narinder Bhari; Thomas M Houslay; Andrew M Pitt; David R Adams; Walter Kolch; Miles D Houslay; Graeme Milligan
Mutations of beta-arrestin 2 that limit self-association also interfere with interactions with the beta2-adrenoceptor and the ERK1/2 MAPKs: implications for beta2-adrenoceptor signalling via the ERK1/2 MAPKs.
The Biochemical journal 2008;413(1):51-60.
2008: Geraldine Parenty; Shirley Appelbe; Graeme Milligan
CXCR2 chemokine receptor antagonism enhances DOP opioid receptor function via allosteric regulation of the CXCR2-DOP receptor heterodimer.
The Biochemical journal 2008;412(2):245-56.
2008: J Robert Lane; David Henderson; Ben Powney; Alan Wise; Steven Rees; Dion Daniels; Chris Plumpton; Ian Kinghorn; Graeme Milligan
Antibodies that identify only the active conformation of G(i) family G protein alpha subunits.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008;22(6):1924-32.
2008: Meritxell Canals; Graeme Milligan
Constitutive activity of the cannabinoid CB1 receptor regulates the function of co-expressed Mu opioid receptors.
The Journal of biological chemistry 2008;283(17):11424-34.
2008: J Robert Lane; Ben Powney; Alan Wise; Steven Rees; Graeme Milligan
G protein coupling and ligand selectivity of the D2L and D3 dopamine receptors.
The Journal of pharmacology and experimental therapeutics 2008;325(1):319-30.
2008: Clare V McCulloch; Valerie Morrow; Sandra Milasta; Iain Comerford; Graeme Milligan; Gerard J Graham; Neil W Isaacs; Robert J B Nibbs
Multiple roles for the C-terminal tail of the chemokine scavenger D6.
The Journal of biological chemistry 2008;283(12):7972-82.
2008: Javier González-Maeso; Rosalind L Ang; Tony Yuen; Pokman Chan; Noelia Weisstaub; Juan F López-Giménez; Mingming Zhou; Yuuya Okawa; Luis F Callado; Graeme Milligan; Jay A Gingrich; Marta Filizola; J Javier Meana; Stuart C Sealfon
Identification of a serotonin/glutamate receptor complex implicated in psychosis.
Nature 2008;452(7183):93-7.
2008: Graeme Milligan
A day in the life of a G protein-coupled receptor: the contribution to function of G protein-coupled receptor dimerization.
British journal of pharmacology 2008;153 Suppl 1():S216-29.
2007: Graeme Milligan; Nicola J Smith
Allosteric modulation of heterodimeric G-protein-coupled receptors.
Trends in pharmacological sciences 2007;28(12):615-20.
2007: Graeme Milligan; Geraldine Parenty; Leigh Stoddart; J Robert Lane
Novel pharmacological applications of G-protein-coupled receptor-G protein fusions.
Current opinion in pharmacology 2007;7(5):521-6.
2007: Nana Sartania; Shirley Appelbe; John D Pediani; Graeme Milligan
Agonist occupancy of a single monomeric element is sufficient to cause internalization of the dimeric beta2-adrenoceptor.
Cellular signalling 2007;19(9):1928-38.
2007: George S Baillie; David R Adams; Narinder Bhari; Thomas M Houslay; Suryakiran Vadrevu; Dong Meng; Xiang Li; Allan J Dunlop; Graeme Milligan; Graeme B Bolger; Enno Klussmann; Miles D Houslay
Mapping binding sites for the PDE4D5 cAMP-specific phosphodiesterase to the N- and C-domains of beta-arrestin using spot-immobilized peptide arrays.
The Biochemical journal 2007;404(1):71-80.
2007: J Robert Lane; Ben Powney; Alan Wise; Steven Rees; Graeme Milligan
Protean agonism at the dopamine D2 receptor: (S)-3-(3-hydroxyphenyl)-N-propylpiperidine is an agonist for activation of Go1 but an antagonist/inverse agonist for Gi1,Gi2, and Gi3.
Molecular pharmacology 2007;71(5):1349-59.
2007: Juan F Lopez-Gimenez; Meritxell Canals; John D Pediani; Graeme Milligan
The alpha1b-adrenoceptor exists as a higher-order oligomer: effective oligomerization is required for receptor maturation, surface delivery, and function.
Molecular pharmacology 2007;71(4):1015-29.
2007: Leigh Stoddart; Andrew J Brown; Graeme Milligan
Uncovering the pharmacology of the G protein-coupled receptor GPR40: high apparent constitutive activity in guanosine 5'-O-(3-[35S]thio)triphosphate binding studies reflects binding of an endogenous agonist.
Molecular pharmacology 2007;71(4):994-1005.
2007: Graeme Milligan
G protein-coupled receptor dimerisation: molecular basis and relevance to function.
Biochimica et biophysica acta 2007;1768(4):825-35.
2006: James Ellis; John D Pediani; Meritxell Canals; Sandra Milasta; Graeme Milligan
Orexin-1 receptor-cannabinoid CB1 receptor heterodimerization results in both ligand-dependent and -independent coordinated alterations of receptor localization and function.
The Journal of biological chemistry 2006;281(50):38812-24.
2006: Graeme Milligan; John D Pediani; Meritxell Canals; Juan F Lopez-Gimenez
Oligomeric structure of the alpha1b-adrenoceptor: comparisons with rhodopsin.
Vision research 2006;46(27):4434-41.
2006: Felix Francke; Richard J Ward; Laura Jenkins; Elaine Kellett; Dietmar Richter; Graeme Milligan; Dietmar Bächner
Interaction of neurochondrin with the melanin-concentrating hormone receptor 1 interferes with G protein-coupled signal transduction but not agonist-mediated internalization.
The Journal of biological chemistry 2006;281(43):32496-507.
2006: Graeme Milligan; Leigh Stoddart; Andrew J Brown
G protein-coupled receptors for free fatty acids.
Cellular signalling 2006;18(9):1360-5.
2006: Jiri Novotny; Dana Durchankova; Richard J Ward; Juan J Carrillo; Petr Svoboda; Graeme Milligan
Functional interactions between the alpha1b-adrenoceptor and Galpha11 are compromised by de-palmitoylation of the G protein but not of the receptor.
Cellular signalling 2006;18(8):1244-51.
2006: Iain Comerford; Sandra Milasta; Valerie Morrow; Graeme Milligan; Robert J B Nibbs
The chemokine receptor CCX-CKR mediates effective scavenging of CCL19 in vitro.
European journal of immunology 2006;36(7):1904-16.
2006: Meritxell Canals; Laura Jenkins; Elaine Kellett; Graeme Milligan
Up-regulation of the angiotensin II type 1 receptor by the MAS proto-oncogene is due to constitutive activation of Gq/G11 by MAS.
The Journal of biological chemistry 2006;281(24):16757-67.
2006: Graeme Milligan
G-protein-coupled receptor heterodimers: pharmacology, function and relevance to drug discovery.
Drug discovery today 2006;11(11-12):541-9.
2006: Francisco Ciruela; Vicent Casadó; Ricardo Jorge Rodrigues; Rafael Luján; Javier Burgueño; Meritxell Canals; Janusz Borycz; Nelson Rebola; Steven R Goldberg; Josefa Mallol; Antonio Cortés; Enric Isidre Canela; Juan F López-Giménez; Graeme Milligan; Carmen Lluis; Rodrigo A Cunha; Sergi Ferré; Rafael Franco
Presynaptic control of striatal glutamatergic neurotransmission by adenosine A1-A2A receptor heteromers.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006;26(7):2080-7.
2006: Sandra Milasta; John D Pediani; Shirley Appelbe; Steven Trim; Michael Wyatt; Peter Cox; Mark Fidock; Graeme Milligan
Interactions between the Mas-related receptors MrgD and MrgE alter signalling and trafficking of MrgD.
Molecular pharmacology 2006;69(2):479-91.
2006: Graeme Milligan; Meritxell Canals; John D Pediani; J Ellis; Juan F Lopez-Gimenez
The role of GPCR dimerisation/oligomerisation in receptor signalling.
Ernst Schering Foundation symposium proceedings 2006;(2):145-61.
2006: Graeme Milligan; Evi Kostenis
Heterotrimeric G-proteins: a short history.
British journal of pharmacology 2006;147 Suppl 1():S46-55.
2005: Richard J Ward; Graeme Milligan
A key serine for the GTPase-activating protein function of regulator of G protein signaling proteins is not a general target for 14-3-3 interactions.
Molecular pharmacology 2005;68(6):1821-30.
2005: Geraldine Pascal; Graeme Milligan
Functional complementation and the analysis of opioid receptor homodimerization.
Molecular pharmacology 2005;68(3):905-15.
2005: Shirley Wilson; Graeme Wilkinson; Graeme Milligan
The CXCR1 and CXCR2 receptors form constitutive homo- and heterodimers selectively and with equal apparent affinities.
The Journal of biological chemistry 2005;280(31):28663-74.
2005: Graeme Milligan; Michel Bouvier
Methods to monitor the quaternary structure of G protein-coupled receptors.
The FEBS journal 2005;272(12):2914-25.
2005: Sandra Milasta; Nicholas A Evans; Laura Ormiston; Shelagh Wilson; Robert J Lefkowitz; Graeme Milligan
The sustainability of interactions between the orexin-1 receptor and beta-arrestin-2 is defined by a single C-terminal cluster of hydroxy amino acids and modulates the kinetics of ERK MAPK regulation.
The Biochemical journal 2005;387(Pt 3):573-84.
2005: John D Pediani; Janet F Colston; Darren Caldwell; Graeme Milligan; Craig J Daly; John C McGrath
Beta-arrestin-dependent spontaneous alpha1a-adrenoceptor endocytosis causes intracellular transportation of alpha-blockers via recycling compartments.
Molecular pharmacology 2005;67(4):992-1004.
2005: Hannah Murdoch; Gui-Jie Feng; Dietmar Bächner; Laura Ormiston; Julia H White; Dietmar Richter; Graeme Milligan
Periplakin interferes with G protein activation by the melanin-concentrating hormone receptor-1 by binding to the proximal segment of the receptor C-terminal tail.
The Journal of biological chemistry 2005;280(9):8208-20.
2005: Graeme Milligan
Opioid receptors and their interacting proteins.
Neuromolecular medicine 2005;7(1-2):51-9.
2005: Graeme Milligan; Shirley Wilson; Juan F López-Gimenez
The specificity and molecular basis of alpha1-adrenoceptor and CXCR chemokine receptor dimerization.
Journal of molecular neuroscience : MN 2005;26(2-3):161-8.
2005: Elaine Barclay; Mark O'Reilly; Graeme Milligan
Activation of an alpha2A-adrenoceptor-Galphao1 fusion protein dynamically regulates the palmitoylation status of the G protein but not of the receptor.
The Biochemical journal 2005;385(Pt 1):197-206.
2004: Graeme Milligan; Hannah Murdoch; Elaine Kellett; Julia H White; Gui-Jie Feng
Interactions between G-protein-coupled receptors and periplakin: a selective means to regulate G-protein activation.
Biochemical Society transactions 2004;32(Pt 5):878-80.
2004: Graeme Milligan; John D Pediani; Mark Fidock; Juan F López-Giménez
Dimerization of alpha1-adrenoceptors.
Biochemical Society transactions 2004;32(Pt 5):847-50.
2004: Juan J Carrillo; Juan F López-Giménez; Graeme Milligan
Multiple interactions between transmembrane helices generate the oligomeric alpha1b-adrenoceptor.
Molecular pharmacology 2004;66(5):1123-37.
2004: Douglas Ramsay; I Craig Carr; John D Pediani; Juan F Lopez-Gimenez; Richard Thurlow; Mark Fidock; Graeme Milligan
High-affinity interactions between human alpha1A-adrenoceptor C-terminal splice variants produce homo- and heterodimers but do not generate the alpha1L-adrenoceptor.
Molecular pharmacology 2004;66(2):228-39.
2004: Graeme Milligan
G protein-coupled receptor dimerization: function and ligand pharmacology.
Molecular pharmacology 2004;66(1):1-7.
2004: Graeme Milligan; Juan F Lopez-Gimenez; Shirley Wilson; Juan J Carrillo
Selectivity in the oligomerisation of G protein-coupled receptors.
Seminars in cell & developmental biology 2004;15(3):263-8.
2004: Graeme Milligan
Applications of bioluminescence- and fluorescence resonance energy transfer to drug discovery at G protein-coupled receptors.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2004;21(4):397-405.
2004: Graeme Milligan; Gui-Jie Feng; Richard J Ward; Nana Sartania; Douglas Ramsay; Alison J McLean; Juan J Carrillo
G protein-coupled receptor fusion proteins in drug discovery.
Current pharmaceutical design 2004;10(17):1989-2001.
2004: Richard J Ward; Graeme Milligan
Analysis of function of receptor-G-protein and receptor-RGS fusion proteins.
Methods in molecular biology (Clifton, N.J.) 2004;259():225-47.
2004: Ian Mullaney; Graeme Milligan
Identification and quantitation of G-protein alpha-subunits.
Methods in molecular biology (Clifton, N.J.) 2004;259():207-24.
2003: Graeme Milligan; Douglas Ramsay; Geraldine Pascal; Juan J Carrillo
GPCR dimerisation.
Life sciences 2003;74(2-3):181-8.
2003: Fu-Yue Zeng; Alison J McLean; Graeme Milligan; Michael Lerner; Derek T Chalmers; Dominic P Behan
Ligand specific up-regulation of a Renilla reniformis luciferase-tagged, structurally unstable muscarinic M3 chimeric G protein-coupled receptor.
Molecular pharmacology 2003;64(6):1474-84.
2003: Graeme Milligan
Constitutive activity and inverse agonists of G protein-coupled receptors: a current perspective.
Molecular pharmacology 2003;64(6):1271-6.
2003: Juan J Carrillo; John D Pediani; Graeme Milligan
Dimers of class A G protein-coupled receptors function via agonist-mediated trans-activation of associated G proteins.
The Journal of biological chemistry 2003;278(43):42578-87.
2003: Gui-Jie Feng; Elaine Kellett; Carol A Scorer; Jonathan Wilde; Julia H White; Graeme Milligan
Selective interactions between helix VIII of the human mu-opioid receptors and the C terminus of periplakin disrupt G protein activation.
The Journal of biological chemistry 2003;278(35):33400-7.
2003: Graeme Milligan
High-content assays for ligand regulation of G-protein-coupled receptors.
Drug discovery today 2003;8(13):579-85.
2003: Daljit S Bahia; Nana Sartania; Richard J Ward; Antonella Cavalli; Teresa L Z Jones; Kirk M Druey; Graeme Milligan
Concerted stimulation and deactivation of pertussis toxin-sensitive G proteins by chimeric G protein-coupled receptor-regulator of G protein signaling 4 fusion proteins: analysis of the contribution of palmitoylated cysteine residues to the GAP activity of RGS4.
Journal of neurochemistry 2003;85(5):1289-98.
2003: Abel Hiol; Penelope C Davey; James L Osterhout; Abdul A Waheed; Elizabeth R Fischer; Ching-Kang Chen; Graeme Milligan; Kirk M Druey; Teresa L Z Jones
Palmitoylation regulates regulators of G-protein signaling (RGS) 16 function. I. Mutation of amino-terminal cysteine residues on RGS16 prevents its targeting to lipid rafts and palmitoylation of an internal cysteine residue.
The Journal of biological chemistry 2003;278(21):19301-8.
2003: James L Osterhout; Abdul A Waheed; Abel Hiol; Richard J Ward; Penelope C Davey; Lylia Nini; Jiun Wang; Graeme Milligan; Teresa L Z Jones; Kirk M Druey
Palmitoylation regulates regulator of G-protein signaling (RGS) 16 function. II. Palmitoylation of a cysteine residue in the RGS box is critical for RGS16 GTPase accelerating activity and regulation of Gi-coupled signalling.
The Journal of biological chemistry 2003;278(21):19309-16.
2003: Alexandrine Derrien; Bin Zheng; James L Osterhout; Yong-Chao Ma; Graeme Milligan; Marilyn G Farquhar; Kirk M Druey
Src-mediated RGS16 tyrosine phosphorylation promotes RGS16 stability.
The Journal of biological chemistry 2003;278(18):16107-16.
2003: Graeme Milligan
Principles: extending the utility of [35S]GTP gamma S binding assays.
Trends in pharmacological sciences 2003;24(2):87-90.
2002: Philip J Welsby; I Craig Carr; Graeme Wilkinson; Graeme Milligan
Regulation of the avidity of ternary complexes containing the human 5-HT(1A) receptor by mutation of a receptor contact site on the interacting G protein alpha subunit.
British journal of pharmacology 2002;137(3):345-52.
2002: Juan J Carrillo; Patricia A Stevens; Graeme Milligan
Measurement of agonist-dependent and -independent signal initiation of alpha(1b)-adrenoceptor mutants by direct analysis of guanine nucleotide exchange on the G protein galpha(11).
The Journal of pharmacology and experimental therapeutics 2002;302(3):1080-8.
2002: Alison J McLean; Fu-Yue Zeng; Dominic P Behan; Derek Chalmers; Graeme Milligan
Generation and analysis of constitutively active and physically destabilized mutants of the human beta(1)-adrenoceptor.
Molecular pharmacology 2002;62(3):747-55.
2002: Graeme Milligan
Strategies to identify ligands for orphan G-protein-coupled receptors.
Biochemical Society transactions 2002;30(4):789-93.
2002: Douglas Ramsay; Elaine Kellett; Mary McVey; Steven Rees; Graeme Milligan
Homo- and hetero-oligomeric interactions between G-protein-coupled receptors in living cells monitored by two variants of bioluminescence resonance energy transfer (BRET): hetero-oligomers between receptor subtypes form more efficiently than between less closely related sequences.
The Biochemical journal 2002;365(Pt 2):429-40.
2002: Sen Liu; Juan J Carrillo; John D Pediani; Graeme Milligan
Effective information transfer from the alpha 1b-adrenoceptor to Galpha 11 requires both beta/gamma interactions and an aromatic group four amino acids from the C terminus of the G protein.
The Journal of biological chemistry 2002;277(28):25707-14.
2002: Philip J Welsby; Elaine Kellett; Graeme Wilkinson; Graeme Milligan
Enhanced detection of receptor constitutive activity in the presence of regulators of G protein signaling: applications to the detection and analysis of inverse agonists and low-efficacy partial agonists.
Molecular pharmacology 2002;61(5):1211-21.
2002: Richard J Ward; Graeme Milligan
Reciprocal mutations of highly conserved residues in transmembrane helices 2 and 7 of the alpha(2A)-adrenoceptor restore agonist activation of G(i1)alpha.
Cellular signalling 2002;14(2):139-44.
2002: Gui-Jie Feng; Antonella Cavalli; Graeme Milligan
Engineering a V(2) vasopressin receptor agonist- and regulator of G-protein-signaling-sensitive G protein.
Analytical biochemistry 2002;300(2):212-20.
2002: Graeme Milligan
The use of receptor G-protein fusion proteins for the study of ligand activity.
Receptors & channels 2002;8(5-6):309-17.
2002: Graeme Milligan; Patricia A Stevens; Douglas Ramsay; Alison J McLean
Ligand rescue of constitutively active mutant receptors.
Neuro-Signals 2002;11(1):29-33.
2002: Graeme Milligan
Construction and analysis of function of G protein-coupled receptor-G protein fusion proteins.
Methods in enzymology 2002;343():260-73.
2001: Graeme Milligan; J H White
Protein-protein interactions at G-protein-coupled receptors.
Trends in pharmacological sciences 2001;22(10):513-8.
2001: Patricia A Stevens; John D Pediani; Juan J Carrillo; Graeme Milligan
Coordinated agonist regulation of receptor and G protein palmitoylation and functional rescue of palmitoylation-deficient mutants of the G protein G11alpha following fusion to the alpha1b-adrenoreceptor: palmitoylation of G11alpha is not required for interaction with beta*gamma complex.
The Journal of biological chemistry 2001;276(38):35883-90.
2001: H E Moon; Daljit S Bahia; Antonella Cavalli; M Hoffmann; Graeme Milligan
Control of the efficiency of agonist-induced information transfer and stability of the ternary complex containing the delta opioid receptor and the alpha subunit of G(i1) by mutation of a receptor/G protein contact interface.
Neuropharmacology 2001;41(3):321-30.
2001: M Hoffmann; Richard J Ward; Antonella Cavalli; I Craig Carr; Graeme Milligan
Differential capacities of the RGS1, RGS16 and RGS-GAIP regulators of G protein signaling to enhance alpha2A-adrenoreceptor agonist-stimulated GTPase activity of G(o1)alpha.
Journal of neurochemistry 2001;78(4):797-806.
2001: Douglas Ramsay; N Bevan; Steven Rees; Graeme Milligan
Detection of receptor ligands by monitoring selective stabilization of a Renilla luciferase-tagged, constitutively active mutant, G-protein-coupled receptor.
British journal of pharmacology 2001;133(2):315-23.
2001: Mary McVey; Douglas Ramsay; Elaine Kellett; Steven Rees; Shelagh Wilson; Andrew J Pope; Graeme Milligan
Monitoring receptor oligomerization using time-resolved fluorescence resonance energy transfer and bioluminescence resonance energy transfer. The human delta -opioid receptor displays constitutive oligomerization at the cell surface, which is not regulated by receptor occupancy.
The Journal of biological chemistry 2001;276(17):14092-9.
2001: Nicholas A Evans; DA Groarke; J Warrack; C J Greenwood; K Dodgson; Graeme Milligan; Shelagh Wilson
Visualizing differences in ligand-induced beta-arrestin-GFP interactions and trafficking between three recently characterized G protein-coupled receptors.
Journal of neurochemistry 2001;77(2):476-85.
2001: H E Moon; Antonella Cavalli; Daljit S Bahia; M Hoffmann; D Massotte; Graeme Milligan
The human delta opioid receptor activates G(i1)alpha more efficiently than G(o1)alpha.
Journal of neurochemistry 2001;76(6):1805-13.
2001: Graeme Milligan; Elaine Kellett; Catherine Dacquet; V Dubreuil; E Jacoby; Mark J Millan; Gilbert Lavielle; Michael Spedding
S 14506: novel receptor coupling at 5-HT(1A) receptors.
Neuropharmacology 2001;40(3):334-44.
2001: DA Groarke; T Drmota; Daljit S Bahia; Nicholas A Evans; Shelagh Wilson; Graeme Milligan
Analysis of the C-terminal tail of the rat thyrotropin-releasing hormone receptor-1 in interactions and cointernalization with beta-arrestin 1-green fluorescent protein.
Molecular pharmacology 2001;59(2):375-85.
2000: Graeme Milligan; A P Ijzerman
Stochastic multidimensional hypercubes and inverse agonism.
Trends in pharmacological sciences 2000;21(10):362-3.
2000: M Sautel; Graeme Milligan
Molecular manipulation of G-protein-coupled receptors: a new avenue into drug discovery.
Current medicinal chemistry 2000;7(9):889-96.
2000: Nabil A Elshourbagy; Robert S Ames; LR Fitzgerald; James J Foley; Jon K Chambers; Philip G Szekeres; Nicholas A Evans; Dulcie B Schmidt; Peter T Buckley; GM Dytko; Paul R Murdock; Graeme Milligan; DA Groarke; Kong B Tan; Usman Shabon; Parvathi Nuthulaganti; D Y Wang; Shelagh Wilson; DJ Bergsma; Henry M Sarau
Receptor for the pain modulatory neuropeptides FF and AF is an orphan G protein-coupled receptor.
The Journal of biological chemistry 2000;275(34):25965-71.
2000: Antonella Cavalli; Kirk M Druey; Graeme Milligan
The regulator of G protein signaling RGS4 selectively enhances alpha 2A-adreoreceptor stimulation of the GTPase activity of Go1alpha and Gi2alpha.
The Journal of biological chemistry 2000;275(31):23693-9.
2000: Alison J McLean; Graeme Milligan
Ligand regulation of green fluorescent protein-tagged forms of the human beta(1)- and beta(2)-adrenoceptors; comparisons with the unmodified receptors.
British journal of pharmacology 2000;130(8):1825-32.
2000: Patricia A Stevens; N Bevan; Steven Rees; Graeme Milligan
Resolution of inverse agonist-induced up-regulation from constitutive activity of mutants of the alpha(1b)-adrenoceptor.
Molecular pharmacology 2000;58(2):438-48.
2000: Graeme Milligan
Neurobiology. Receptors as kissing cousins.
Science (New York, N.Y.) 2000;288(5463):65-7.
2000: T Drmota; Graeme Milligan
Kinetic analysis of the internalization and recycling of [3H]TRH and C-terminal truncations of the long isoform of the rat thyrotropin-releasing hormone receptor-1.
The Biochemical journal 2000;346 Pt 3():711-8.
2000: Graeme Milligan
Insights into ligand pharmacology using receptor-G-protein fusion proteins.
Trends in pharmacological sciences 2000;21(1):24-8.
1999: Richard J Ward; Graeme Milligan
An Asp79Asn mutation of the alpha2A-adrenoceptor interferes equally with agonist activation of individual Gialpha-family G protein subtypes.
FEBS letters 1999;462(3):459-63.
1999: Alison J McLean; N Bevan; Steven Rees; Graeme Milligan
Visualizing differences in ligand regulation of wild-type and constitutively active mutant beta(2)-adrenoceptor-green fluorescent protein fusion proteins.
Molecular pharmacology 1999;56(6):1182-91.
1999: N Bevan; T Palmer; T Drmota; A Wise; J Coote; Graeme Milligan; Steven Rees
Functional analysis of a human A(1) adenosine receptor/green fluorescent protein/G(i1)alpha fusion protein following stable expression in CHO cells.
FEBS letters 1999;462(1-2):61-5.
1999: Graeme Milligan
Exploring the dynamics of regulation of G protein-coupled receptors using green fluorescent protein.
British journal of pharmacology 1999;128(3):501-10.
1999: Elaine Kellett; I Craig Carr; Graeme Milligan
Regulation of G protein activation and effector modulation by fusion proteins between the human 5-hydroxytryptamine(1A) receptor and the alpha subunit of G(i1): differences in receptor-constitutive activity imparted by single amino acid substitutions in G(i1)alpha.
Molecular pharmacology 1999;56(4):684-92.
1999: CW Fong; Graeme Milligan
Analysis of agonist function at fusion proteins between the IP prostanoid receptor and cognate, unnatural and chimaeric G-proteins.
The Biochemical journal 1999;342 ( Pt 2)():457-63.
1999: DA Groarke; Shelagh Wilson; C Krasel; Graeme Milligan
Visualization of agonist-induced association and trafficking of green fluorescent protein-tagged forms of both beta-arrestin-1 and the thyrotropin-releasing hormone receptor-1.
The Journal of biological chemistry 1999;274(33):23263-9.
1999: T Drmota; J Novotny; Gwyn W Gould; Petr Svoboda; Graeme Milligan
Visualization of distinct patterns of subcellular redistribution of the thyrotropin-releasing hormone receptor-1 and gqalpha /G11alpha induced by agonist stimulation.
The Biochemical journal 1999;340 ( Pt 2)():529-38.
1999: Graeme Milligan; S Rees
Chimaeric G alpha proteins: their potential use in drug discovery.
Trends in pharmacological sciences 1999;20(3):118-24.
1999: Graeme Milligan; DA Groarke; A McLean; R Ward; CW Fong; A Cavalli; T Drmota
Diversity in the signalling and regulation of G-protein-coupled receptors.
Biochemical Society transactions 1999;27(2):149-54.
1999: V N Jackson; Daljit S Bahia; Graeme Milligan
Modulation of relative intrinsic activity of agonists at the alpha-2A adrenoceptor by mutation of residue 351 of G protein gi1alpha.
Molecular pharmacology 1999;55(2):195-201.
1998: M Sautel; Graeme Milligan
Loss of activation of Gs but not Gi following expression of an alpha2A-adrenoceptor-Gi1alpha fusion protein.
FEBS letters 1998;436(1):46-50.
1998: T Drmota; Gwyn W Gould; Graeme Milligan
Real time visualization of agonist-mediated redistribution and internalization of a green fluorescent protein-tagged form of the thyrotropin-releasing hormone receptor.
The Journal of biological chemistry 1998;273(37):24000-8.
1998: T Drmota; J Novotny; G D Kim; K A Eidne; Graeme Milligan; Petr Svoboda
Agonist-induced internalization of the G protein G11alpha and thyrotropin-releasing hormone receptors proceed on different time scales.
The Journal of biological chemistry 1998;273(34):21699-707.
1998: Daljit S Bahia; Alan Wise; F Fanelli; M Lee; Steven Rees; Graeme Milligan
Hydrophobicity of residue351 of the G protein Gi1 alpha determines the extent of activation by the alpha 2A-adrenoceptor.
Biochemistry 1998;37(33):11555-62.
1998: Craig J Daly; C M Milligan; Graeme Milligan; JF Mackenzie; John C McGrath
Cellular localization and pharmacological characterization of functioning alpha-1 adrenoceptors by fluorescent ligand binding and image analysis reveals identical binding properties of clustered and diffuse populations of receptors.
The Journal of pharmacology and experimental therapeutics 1998;286(2):984-90.
1998: CW Fong; Daljit S Bahia; Steven Rees; Graeme Milligan
Selective activation of a chimeric Gi1/Gs G protein alpha subunit by the human IP prostanoid receptor: analysis using agonist stimulation of high affinity GTPase activity and [35S]guanosine-5'-O-(3-thio)triphosphate binding.
Molecular pharmacology 1998;54(2):249-57.
1998: I Craig Carr; AR Burt; V N Jackson; J Wright; Alan Wise; Steven Rees; Graeme Milligan
Quantitative analysis of a cysteine351glycine mutation in the G protein Gi1alpha: effect on alpha2A-adrenoceptor-Gi1alpha fusion protein activation.
FEBS letters 1998;428(1-2):17-22.
1998: AR Burt; M Sautel; M A Wilson; Steven Rees; Alan Wise; Graeme Milligan
Agonist occupation of an alpha2A-adrenoreceptor-Gi1alpha fusion protein results in activation of both receptor-linked and endogenous Gi proteins. Comparisons of their contributions to GTPase activity and signal transduction and analysis of receptor-G protein activation stoichiometry.
The Journal of biological chemistry 1998;273(17):10367-75.
1998: Graeme Milligan
New aspects of G-protein-coupled receptor signalling and regulation.
Trends in endocrinology and metabolism: TEM 1998;9(1):13-9.
1998: MA Grassie; Graeme Milligan
Agonist-mediated turnover of G-protein alpha-subunit palmitoyl groups. Role in membrane insertion.
Methods in molecular biology (Clifton, N.J.) 1998;88():241-8.
1998: MA Grassie; Graeme Milligan
Molecular genetic approaches. IV. Recombinant expression of wild-type and acylation-resistant G-protein alpha-subunits using transient transfection systems.
Methods in molecular biology (Clifton, N.J.) 1998;88():151-60.
1998: Patricia A Stevens; Graeme Milligan
Efficacy of inverse agonists in cells overexpressing a constitutively active beta2-adrenoceptor and type II adenylyl cyclase.
British journal of pharmacology 1998;123(2):335-43.
1998: Graeme Milligan; Ian Mullaney; G D Kim; D MacEwan
Regulation of the stoichiometry of protein components of the stimulatory adenylyl cyclase cascade.
Advances in pharmacology (San Diego, Calif.) 1998;42():462-5.
1997: Zafiroula Georgoussi; Manolis Merkouris; Ian Mullaney; G Megaritis; C Carr; C Zioudrou; Graeme Milligan
Selective interactions of mu-opioid receptors with pertussis toxin-sensitive G proteins: involvement of the third intracellular loop and the c-terminal tail in coupling.
Biochimica et biophysica acta 1997;1359(3):263-74.
1997: Alan Wise; I Craig Carr; DA Groarke; Graeme Milligan
Measurement of agonist efficacy using an alpha2A-adrenoceptor-Gi1alpha fusion protein.
FEBS letters 1997;419(1):141-6.
1997: Graeme Milligan; R A Bond
Inverse agonism and the regulation of receptor number.
Trends in pharmacological sciences 1997;18(12):468-74.
1997: Manolis Merkouris; Ian Mullaney; Zafiroula Georgoussi; Graeme Milligan
Regulation of spontaneous activity of the delta-opioid receptor: studies of inverse agonism in intact cells.
Journal of neurochemistry 1997;69(5):2115-22.
1997: Alan Wise; Graeme Milligan
Rescue of functional interactions between the alpha2A-adrenoreceptor and acylation-resistant forms of Gi1alpha by expressing the proteins from chimeric open reading frames.
The Journal of biological chemistry 1997;272(39):24673-8.
1997: Alan Wise; MA Grassie; M Parenti; M Lee; Steven Rees; Graeme Milligan
A cysteine-3 to serine mutation of the G-protein Gi1 alpha abrogates functional activation by the alpha 2A-adrenoceptor but not interactions with the beta gamma complex.
Biochemistry 1997;36(35):10620-9.
1997: Ian Mullaney; I Craig Carr; AR Burt; M Wilson; NG Anderson; Graeme Milligan
Agonist-mediated tyrosine phosphorylation of isoforms of the shc adapter protein by the delta opioid receptor.
Cellular signalling 1997;9(6):423-9.
1997: T W Lee; S Cotecchia; Graeme Milligan
Up-regulation of the levels of expression and function of a constitutively active mutant of the hamster alpha1B-adrenoceptor by ligands that act as inverse agonists.
The Biochemical journal 1997;325 ( Pt 3)():733-9.
1997: M A Wilson; AR Burt; Graeme Milligan; NG Anderson
Mitogenic signalling by delta opioid receptors expressed in rat-1 fibroblasts involves activation of the p70s6k/p85s6k S6 kinase.
The Biochemical journal 1997;325 ( Pt 1)():217-22.
1997: Alan Wise; I Craig Carr; Graeme Milligan
Measurement of agonist-induced guanine nucleotide turnover by the G-protein Gi1alpha when constrained within an alpha2A-adrenoceptor-Gi1alpha fusion protein.
The Biochemical journal 1997;325 ( Pt 1)():17-21.
1997: Miles D Houslay; Graeme Milligan
Tailoring cAMP-signalling responses through isoform multiplicity.
Trends in biochemical sciences 1997;22(6):217-24.
1997: Alan Wise; M Parenti; Graeme Milligan
Interaction of the G-protein G11alpha with receptors and phosphoinositidase C: the contribution of G-protein palmitoylation and membrane association.
FEBS letters 1997;407(3):257-60.
1997: Alan Wise; M A Watson-Koken; Steven Rees; M Lee; Graeme Milligan
Interactions of the alpha2A-adrenoceptor with multiple Gi-family G-proteins: studies with pertussis toxin-resistant G-protein mutants.
The Biochemical journal 1997;321 ( Pt 3)():721-8.
1997: Graeme Milligan; D J MacEwan; M Mercouris; Ian Mullaney
Inverse agonism at adrenergic and opioid receptors: studies with wild type and constitutively active mutant receptors.
Receptors & channels 1997;5(3-4):209-13.
1997: Graeme Milligan; MA Grassie
How do G-proteins stay at the plasma membrane?
Essays in biochemistry 1997;32():49-60.
1997: Ian Mullaney; Graeme Milligan
Identification and quantitation of G protein alpha-subunits.
Methods in molecular biology (Clifton, N.J.) 1997;83():159-77.
1996: D J MacEwan; Graeme Milligan
Up-regulation of a constitutively active form of the beta2-adrenoceptor by sustained treatment with inverse agonists but not antagonists.
FEBS letters 1996;399(1-2):108-12.
1996: D J MacEwan; Graeme Milligan
Inverse agonist-induced up-regulation of the human beta2-adrenoceptor in transfected neuroblastoma X glioma hybrid cells.
Molecular pharmacology 1996;50(6):1479-86.
1996: Ian Mullaney; I Craig Carr; Graeme Milligan
Overexpression of G(s)alpha in NG108-15, neuroblastomaXglioma cells: effects on receptor regulation of the stimulatory adenylyl cyclase cascade.
FEBS letters 1996;397(2-3):325-30.
1996: AR Burt; I Craig Carr; Ian Mullaney; NG Anderson; Graeme Milligan
Agonist activation of p42 and p44 mitogen-activated protein kinases following expression of the mouse delta opioid receptor in Rat-1 fibroblasts: effects of receptor expression levels and comparisons with G-protein activation.
The Biochemical journal 1996;320 ( Pt 1)():227-35.
1996: T W Lee; Alan Wise; S Cotecchia; Graeme Milligan
A constitutively active mutant of the alpha 1B-adrenergic receptor can cause greater agonist-dependent down-regulation of the G-proteins G9 alpha and G11 alpha than the wild-type receptor.
The Biochemical journal 1996;320 ( Pt 1)():79-86.
1996: Manolis Merkouris; I Dragatsis; G Megaritis; G Konidakis; C Zioudrou; Graeme Milligan; Zafiroula Georgoussi
Identification of the critical domains of the delta-opioid receptor involved in G protein coupling using site-specific synthetic peptides.
Molecular pharmacology 1996;50(4):985-93.
1996: D J MacEwan; G D Kim; Graeme Milligan
Agonist regulation of adenylate cyclase activity in neuroblastoma x glioma hybrid NG108-15 cells transfected to co-express adenylate cyclase type II and the beta 2-adrenoceptor. Evidence that adenylate cyclase is the limiting component for receptor-mediated stimulation of adenylate cyclase activity.
The Biochemical journal 1996;318 ( Pt 3)():1033-9.
1996: Graeme Milligan; F Marshall; S Rees
G16 as a universal G protein adapter: implications for agonist screening strategies.
Trends in pharmacological sciences 1996;17(7):235-7.
1996: M Wilson; AR Burt; Graeme Milligan; NG Anderson
Wortmannin-sensitive activation of p70s6k by endogenous and heterologously expressed Gi-coupled receptors.
The Journal of biological chemistry 1996;271(15):8537-40.
1996: Ian Mullaney; I Craig Carr; Graeme Milligan
Analysis of inverse agonism at the delta opioid receptor after expression in Rat 1 fibroblasts.
The Biochemical journal 1996;315 ( Pt 1)():227-34.
1996: Petr Svoboda; G D Kim; MA Grassie; Karin A Eidne; Graeme Milligan
Thyrotropin-releasing hormone-induced subcellular redistribution and down-regulation of G11alpha: analysis of agonist regulation of coexpressed G11alpha species variants.
Molecular pharmacology 1996;49(4):646-55.
1996: Graeme Milligan
The stoichiometry of expression of protein components of the stimulatory adenylyl cyclase cascade and the regulation of information transfer.
Cellular signalling 1996;8(2):87-95.
1996: F Galbiati; F Guzzi; A I Magee; Graeme Milligan; Marco Parenti
Chemical inhibition of myristoylation of the G-protein Gi1 alpha by 2-hydroxymyristate does not interfere with its palmitoylation or membrane association. Evidence that palmitoylation, but not myristoylation, regulates membrane attachment.
The Biochemical journal 1996;313 ( Pt 3)():717-20.
1995: J F McCallum; Alan Wise; MA Grassie; A I Magee; F Guzzi; Marco Parenti; Graeme Milligan
The role of palmitoylation of the guanine nucleotide binding protein G11 alpha in defining interaction with the plasma membrane.
The Biochemical journal 1995;310 ( Pt 3)():1021-7.
1995: T W Lee; L A Anderson; Karin A Eidne; Graeme Milligan
Comparison of the signalling properties of the long and short isoforms of the rat thyrotropin-releasing-hormone receptor following expression in rat 1 fibroblasts.
The Biochemical journal 1995;310 ( Pt 1)():291-8.
1995: Graeme Milligan; G D Kim; Ian Mullaney; E J Adie
Regulation of cellular Gs alpha levels and basal adenylyl cyclase activity by expression of the beta 2-adrenoceptor in neuroblastoma cell lines.
Molecular and cellular biochemistry 1995;149-150():213-6.
1995: Graeme Milligan; MA Grassie; Alan Wise; D J MacEwan; A I Magee; Marco Parenti
G-protein palmitoylation: regulation and functional significance.
Biochemical Society transactions 1995;23(3):583-7.
1995: MA Grassie; Graeme Milligan
G(o1) alpha palmitoylation: its contribution to alpha 2C10 adrenoceptor--G(o1) alpha coupling.
Biochemical Society transactions 1995;23(3):406S.
1995: D J MacEwan; G D Kim; Graeme Milligan
Analysis of the role of receptor number in defining the intrinsic activity and potency of partial agonists in neuroblastoma x glioma hybrid NG108-15 cells transfected to express differing levels of the human beta 2-adrenoceptor.
Molecular pharmacology 1995;48(2):316-25.
1995: Ian Mullaney; B H Shah; A Wise; Graeme Milligan
Expression of the human beta 2-adrenoceptor in NCB20 cells results in agonist activation of adenylyl cyclase and agonist-mediated selective down-regulation of Gs alpha.
Journal of neurochemistry 1995;65(2):545-53.
1995: Alan Wise; T W Lee; D J MacEwan; Graeme Milligan
Degradation of G11 alpha/Gq alpha is accelerated by agonist occupancy of alpha 1A/D, alpha 1B, and alpha 1C adrenergic receptors.
The Journal of biological chemistry 1995;270(29):17196-203.
1995: G D Kim; I Craig Carr; Graeme Milligan
Detection and analysis of agonist-induced formation of the complex of the stimulatory guanine nucleotide-binding protein with adenylate cyclase in intact wild-type and beta 2-adrenoceptor-expressing NG108-15 cells.
The Biochemical journal 1995;308 ( Pt 1)():275-81.
1995: Graeme Milligan; Marco Parenti; A I Magee
The dynamic role of palmitoylation in signal transduction.
Trends in biochemical sciences 1995;20(5):181-7.
1995: B H Shah; D J MacEwan; Graeme Milligan
Gonadotrophin-releasing hormone receptor agonist-mediated down-regulation of Gq alpha/G11 alpha (pertussis toxin-insensitive) G proteins in alpha T3-1 gonadotroph cells reflects increased G protein turnover but not alterations in mRNA levels.
Proceedings of the National Academy of Sciences of the United States of America 1995;92(6):1886-90.
1995: MA Grassie; Graeme Milligan
Analysis of the relative interactions between the alpha 2C10 adrenoceptor and the guanine-nucleotide-binding proteins G(o)1 alpha and Gi 2 alpha following co-expression of these polypeptides in rat 1 fibroblasts.
The Biochemical journal 1995;306 ( Pt 2)():525-30.
1995: Zafiroula Georgoussi; Graeme Milligan; C Zioudrou
Immunoprecipitation of opioid receptor-Go-protein complexes using specific GTP-binding-protein antisera.
The Biochemical journal 1995;306 ( Pt 1)():71-5.
1995: J F McCallum; Alan Wise; Marco Parenti; Graeme Milligan
Palmitoylation negative mutants of murine G11 alpha have decreased ability to interact with the plasma membrane when expressed in COS-1 cells.
Biochemical Society transactions 1995;23(1):9S.
1995: Graeme Milligan; Alan Wise; D J MacEwan; MA Grassie; F R Kennedy; T W Lee; E J Adie; G D Kim; J F McCallum; A Burt
Mechanisms of agonist-induced G-protein elimination.
Biochemical Society transactions 1995;23(1):166-70.
1995: Alan Wise; Graeme Milligan
High level expression of mammalian G protein alpha subunit Gq subtypes in Escherichia coli.
Biochemical Society transactions 1995;23(1):12S.
1995: T W Lee; Karin A Eidne; Graeme Milligan
Signalling characteristics of thyrotropin releasing hormone (TRH) receptor isoforms.
Biochemical Society transactions 1995;23(1):11S.
1995: B H Shah; Graeme Milligan
Cyclic AMP differentially regulates the expression of the alpha-subunits of Gs, Gq and G11 G-proteins and their mRNA levels in rat C6 glioma cells.
Biochemical Society transactions 1995;23(1):10S.
1995: G D Kim; Graeme Milligan
Agonist regulation of high affinity [3H] forskolin binding as a measure of GS alpha-adenylyl cyclase interactions.
Biochemical Society transactions 1995;23(1):8S.
1995: Ian Mullaney; Graeme Milligan
Interaction of the beta 2-adrenoceptor with epitope-tagged Gs alpha in NG108-15 cells.
Biochemical Society transactions 1995;23(1):7S.
1995: Graeme Milligan; B H Shah; Ian Mullaney; MA Grassie
Biochemical approaches to examine the specificity of interactions between receptors and guanine nuclotide binding proteins.
Journal of receptor and signal transduction research 1995;15(1-4):253-65.
1995: Graeme Milligan
Signal sorting by G-protein-linked receptors.
Advances in pharmacology (San Diego, Calif.) 1995;32():1-29.
1995: Graeme Milligan; R A Bond; M Lee
Inverse agonism: pharmacological curiosity or potential therapeutic strategy?
Trends in pharmacological sciences 1995;16(1):10-3.
1994: G D Kim; Graeme Milligan
Receptor availability defines the extent of agonist-mediated G-protein down-regulation in neuroblastoma x glioma hybrid cells transfected to express the beta 2-adrenoceptor.
FEBS letters 1994;355(2):166-70.
1994: E J Adie; Graeme Milligan
Regulation of basal adenylate cyclase activity in neuroblastoma x glioma hybrid, NG108-15, cells transfected to express the human beta 2 adrenoceptor: evidence for empty receptor stimulation of the adenylate cyclase cascade.
The Biochemical journal 1994;303 ( Pt 3)():803-8.
1994: F Galbiati; F Guzzi; A I Magee; Graeme Milligan; Marco Parenti
N-terminal fatty acylation of the alpha-subunit of the G-protein Gi1: only the myristoylated protein is a substrate for palmitoylation.
The Biochemical journal 1994;303 ( Pt 3)():697-700.
1994: Ian Mullaney; Graeme Milligan
Equivalent regulation of wild type and an epitope-tagged variant of Gs alpha by the IP prostanoid receptor following expression in neuroblastoma x glioma hybrid, NG108-15, cells.
FEBS letters 1994;353(3):231-4.
1994: MA Grassie; J F McCallum; F Guzzi; A I Magee; Graeme Milligan; Marco Parenti
The palmitoylation status of the G-protein G(o)1 alpha regulates its activity of interaction with the plasma membrane.
The Biochemical journal 1994;302 ( Pt 3)():913-20.
1994: Petr Svoboda; Graeme Milligan
Agonist-induced transfer of the alpha subunits of the guanine-nucleotide-binding regulatory proteins Gq and G11 and of muscarinic m1 acetylcholine receptors from plasma membranes to a light-vesicular membrane fraction.
European journal of biochemistry / FEBS 1994;224(2):455-62.
1994: G D Kim; I Craig Carr; L A Anderson; J Zabavnik; Karin A Eidne; Graeme Milligan
The long isoform of the rat thyrotropin-releasing hormone receptor down-regulates Gq proteins.
The Journal of biological chemistry 1994;269(31):19933-40.
1994: G D Kim; Graeme Milligan
Concurrent specific immunological detection of both primate and rodent forms of the guanine nucleotide binding protein G11 alpha following their coexpression.
Biochimica et biophysica acta 1994;1222(3):369-74.
1994: B H Shah; Graeme Milligan
The gonadotrophin-releasing hormone receptor of alpha T3-1 pituitary cells regulates cellular levels of both of the phosphoinositidase C-linked G proteins, Gq alpha and G11 alpha, equally.
Molecular pharmacology 1994;46(1):1-7.
1994: E J Adie; Graeme Milligan
Agonist regulation of cellular Gs alpha-subunit levels in neuroblastoma x glioma hybrid NG108-15 cells transfected to express different levels of the human beta 2 adrenoceptor.
The Biochemical journal 1994;300 ( Pt 3)():709-15.
1994: C Carr; MA Grassie; Graeme Milligan
Stimulation of high-affinity GTPase activity and cholera toxin-catalysed [32P]ADP-ribosylation of Gi by lysophosphatidic acid (LPA) in wild-type and alpha 2C10 adrenoceptor-transfected Rat 1 fibroblasts.
The Biochemical journal 1994;298 ( Pt 2)():493-7.
1994: G D Kim; E J Adie; Graeme Milligan
Quantitative stoichiometry of the proteins of the stimulatory arm of the adenylyl cyclase cascade in neuroblastoma x glioma hybrid, NG108-15 cells.
European journal of biochemistry / FEBS 1994;219(1-2):135-43.
1994: Graeme Milligan
Specificity and functional applications of antipeptide antisera which identify G-protein alpha subunits.
Methods in enzymology 1994;237():268-83.
1993: Graeme Milligan; Ian Mullaney; J F McCallum
Distribution and relative levels of expression of the phosphoinositidase-C-linked G-proteins Gq alpha and G11 alpha: absence of G11 alpha in human platelets and haemopoietically derived cell lines.
Biochimica et biophysica acta 1993;1179(2):208-12.
1993: Graeme Milligan
Agonist regulation of cellular G protein levels and distribution: mechanisms and functional implications.
Trends in pharmacological sciences 1993;14(11):413-8.
1993: MA Grassie; J F McCallum; Marco Parenti; A I Magee; Graeme Milligan
Lack of N terminal palmitoylation of G protein alpha subunits reduces membrane association.
Biochemical Society transactions 1993;21(4):499S.
1993: B H Shah; Graeme Milligan
Activation of the gonadotrophin releasing hormone receptor of alpha T3 cells results in downregulation of the alpha subunits of both Gq/G11.
Biochemical Society transactions 1993;21(4):498S.
1993: Ian Mullaney; Graeme Milligan
Agonist activation of transfected human M1 muscarinic acetylcholine receptor in Chinese hamster ovary cells results in concurrent downregulation of Gq alpha and G11 alpha.
Biochemical Society transactions 1993;21(4):497S.
1993: Brian M Ross; M McLaughlin; M Roberts; Graeme Milligan; James McCulloch; J T Knowler
Alterations in the activity of adenylate cyclase and high affinity GTPase in Alzheimer's disease.
Brain research 1993;622(1-2):35-42.
1993: Graeme Milligan
Regional distribution and quantitative measurement of the phosphoinositidase C-linked guanine nucleotide binding proteins G11 alpha and Gq alpha in rat brain.
Journal of neurochemistry 1993;61(3):845-51.
1993: F M Mitchell; N J Buckley; Graeme Milligan
Enhanced degradation of the phosphoinositidase C-linked guanine-nucleotide-binding protein Gq alpha/G11 alpha following activation of the human M1 muscarinic acetylcholine receptor expressed in CHO cells.
The Biochemical journal 1993;293 ( Pt 2)():495-9.
1993: Graeme Milligan; F M Mitchell
An arginine residue is the site of receptor-stimulated, cholera toxin-catalysed ADP-ribosylation of pertussis toxin-sensitive G-proteins.
Cellular signalling 1993;5(4):485-93.
1993: Zafiroula Georgoussi; C Carr; Graeme Milligan
Direct measurements of in situ interactions of rat brain opioid receptors with the guanine nucleotide-binding protein Go.
Molecular pharmacology 1993;44(1):62-9.
1993: Ian Mullaney; F M Mitchell; J F McCallum; N J Buckley; Graeme Milligan
The human muscarinic M1 acetylcholine receptor, when express in CHO cells, activates and downregulates both Gq alpha and G11 alpha equally and non-selectively.
FEBS letters 1993;324(2):241-5.
1993: Graeme Milligan
Mechanisms of multifunctional signalling by G protein-linked receptors.
Trends in pharmacological sciences 1993;14(6):239-44.
1993: A M Brant; Thomas Jess; Graeme Milligan; C M Brown; G W Gould
Immunological analysis of glucose transporters expressed in different regions of the rat brain and central nervous system.
Biochemical and biophysical research communications 1993;192(3):1297-302.
1993: E J Adie; Graeme Milligan
Agonist regulation of cellular levels of the stimulatory guanine nucleotide-binding protein, Gs, in wild type and transfected neuroblastoma-glioma hybrid NG108-15 cells.
Biochemical Society transactions 1993;21(2):432-5.
1993: Ian Mullaney; M W Dodd; N Buckley; Graeme Milligan
Agonist activation of transfected human M1 muscarinic acetylcholine receptors in CHO cells results in down-regulation of both the receptor and the alpha subunit of the G-protein Gq.
The Biochemical journal 1993;289 ( Pt 1)():125-31.
1992: E J Adie; Ian Mullaney; F R McKenzie; Graeme Milligan
Concurrent down-regulation of IP prostanoid receptors and the alpha-subunit of the stimulatory guanine-nucleotide-binding protein (Gs) during prolonged exposure of neuroblastoma x glioma cells to prostanoid agonists. Quantification and functional implications.
The Biochemical journal 1992;285 ( Pt 2)():529-36.
1992: Steven McClue; Edgar Selzer; Michael Freissmuth; Graeme Milligan
Gi3 does not contribute to the inhibition of adenylate cyclase when stimulation of an alpha 2-adrenergic receptor causes activation of both Gi2 and Gi3.
The Biochemical journal 1992;284 ( Pt 2)():565-8.
1992: D Strassheim; Miles D Houslay; Graeme Milligan
Regulation of cardiac adenylate cyclase activity in rodent models of obesity.
The Biochemical journal 1992;283 ( Pt 1)():203-8.
1992: E E MacNulty; Steven McClue; I Craig Carr; Thomas Jess; Michael J O Wakelam; Graeme Milligan
Alpha 2-C10 adrenergic receptors expressed in rat 1 fibroblasts can regulate both adenylylcyclase and phospholipase D-mediated hydrolysis of phosphatidylcholine by interacting with pertussis toxin-sensitive guanine nucleotide-binding proteins.
The Journal of biological chemistry 1992;267(4):2149-56.
1992: Graeme Milligan; Ian Mullaney; F M Mitchell
Immunological identification of the alpha subunit of G13, a novel guanine nucleotide binding protein.
FEBS letters 1992;297(1-2):186-8.
1992: Graeme Milligan
Multiple heterotrimeric guanine nucleotide binding proteins: roles in the determination of cellular signalling specificity.
Biochemical Society transactions 1992;20(1):135-40.
1991: Steven McClue; Graeme Milligan
Molecular interaction of the human alpha 2-C10-adrenergic receptor, when expressed in Rat-1 fibroblasts, with multiple pertussis toxin-sensitive guanine nucleotide-binding proteins: studies with site-directed antisera.
Molecular pharmacology 1991;40(5):627-32.
1991: F M Mitchell; Ian Mullaney; P P Godfrey; Steve Arkinstall; Michael J O Wakelam; Graeme Milligan
Widespread distribution of Gq alpha/G11 alpha detected immunologically by an antipeptide antiserum directed against the predicted C-terminal decapeptide.
FEBS letters 1991;287(1-2):171-4.
1991: M McLaughlin; Brian M Ross; Graeme Milligan; James McCulloch; J T Knowler
Robustness of G proteins in Alzheimer's disease: an immunoblot study.
Journal of neurochemistry 1991;57(1):9-14.
1991: Graeme Milligan; A Green
Agonist control of G-protein levels.
Trends in pharmacological sciences 1991;12(6):207-9.
1991: D Strassheim; T Palmer; Graeme Milligan; Miles D Houslay
Alterations in G-protein expression and the hormonal regulation of adenylate cyclase in the adipocytes of obese (fa/fa) Zucker rats.
The Biochemical journal 1991;276 ( Pt 1)():197-202.
1991: Graeme Milligan; C Carr; G W Gould; Ian Mullaney; B E Lavan
Agonist-dependent, cholera toxin-catalyzed ADP-ribosylation of pertussis toxin-sensitive G-proteins following transfection of the human alpha 2-C10 adrenergic receptor into rat 1 fibroblasts. Evidence for the direct interaction of a single receptor with two pertussis toxin-sensitive G-proteins, Gi2 and Gi3.
The Journal of biological chemistry 1991;266(10):6447-55.
1991: F M Mitchell; Graeme Milligan
U937 cell differentiation: G protein alterations and interaction with the chemotactic peptide receptor.
Biochemical Society transactions 1991;19(2):98S.
1991: F R McKenzie; E J Adie; Graeme Milligan
Prostanoid-mediated downregulation of Gs in NG108-15 cells.
Biochemical Society transactions 1991;19(2):81S.
1991: Graeme Milligan; E E MacNulty; Steven McClue; Michael J O Wakelam
Signal transduction in Rat-1 fibroblasts expressing high levels of the alpha 2-C10 adrenergic receptor.
Biochemical Society transactions 1991;19(2):102S.
1991: F R McKenzie; Graeme Milligan
Cholera toxin impairment of opioid-mediated inhibition of adenylate cyclase in neuroblastoma x glioma hybrid cells is due to a toxin-induced decrease in opioid receptor levels.
The Biochemical journal 1991;275 ( Pt 1)():175-81.
1991: C Livingstone; A R McLellan; M A McGregor; A Wilson; J M Connell; M Small; Graeme Milligan; K R Paterson; Miles D Houslay
Altered G-protein expression and adenylate cyclase activity in platelets of non-insulin-dependent diabetic (NIDDM) male subjects.
Biochimica et biophysica acta 1991;1096(2):127-33.
1991: Z Tanfin; O Goureau; Graeme Milligan; Simone Harbon
Characterization of G proteins in rat myometrium. A differential modulation of Gi2 alpha and Gi3 alpha during gestation.
FEBS letters 1991;278(1):4-8.
1990: Ian Mullaney; Graeme Milligan
Identification of two distinct isoforms of the guanine nucleotide binding protein G0 in neuroblastoma X glioma hybrid cells: independent regulation during cyclic AMP-induced differentiation.
Journal of neurochemistry 1990;55(6):1890-8.
1990: M Bushfield; S L Griffiths; G J Murphy; N J Pyne; J T Knowler; Graeme Milligan; P J Parker; S Mollner; Miles D Houslay
Diabetes-induced alterations in the expression, functioning and phosphorylation state of the inhibitory guanine nucleotide regulatory protein Gi-2 in hepatocytes.
The Biochemical journal 1990;271(2):365-72.
1990: F R McKenzie; Graeme Milligan
Prostaglandin E1-mediated, cyclic AMP-independent, down-regulation of Gs alpha in neuroblastoma x glioma hybrid cells.
The Journal of biological chemistry 1990;265(28):17084-93.
1990: Graeme Milligan; E D Saggerson
Concurrent up-regulation of guanine-nucleotide-binding proteins Gi1 alpha, Gi2 alpha and Gi3 alpha in adipocytes of hypothyroid rats.
The Biochemical journal 1990;270(3):765-9.
1990: Steven McClue; Graeme Milligan
The alpha 2B adrenergic receptor of undifferentiated neuroblastoma x glioma hybrid NG108-15 cells, interacts directly with the guanine nucleotide binding protein, Gi2.
FEBS letters 1990;269(2):430-4.
1990: C A Evans; Graeme Milligan; Michael J O Wakelam
Effect of muscle cell differentiation upon G-protein levels.
Biochemical Society transactions 1990;18(3):481-2.
1990: Ian Mullaney; Graeme Milligan
Identification and analysis of two distinct isoforms of the guanine nucleotide-binding protein Go in NG108-15 cells.
Biochemical Society transactions 1990;18(3):396-9.
1990: M Bushfield; G J Murphy; B E Lavan; P J Parker; V J Hruby; Graeme Milligan; Miles D Houslay
Hormonal regulation of Gi2 alpha-subunit phosphorylation in intact hepatocytes.
The Biochemical journal 1990;268(2):449-57.
1990: C Carr; C Loney; Cecilia G Unson; J Knowler; Graeme Milligan
Chronic exposure of rat glioma C6 cells to cholera toxin induces loss of the alpha-subunit of the stimulatory guanine nucleotide-binding protein (Gs).
European journal of pharmacology 1990;188(4-5):203-9.
1990: F R McKenzie; Graeme Milligan
Delta-opioid-receptor-mediated inhibition of adenylate cyclase is transduced specifically by the guanine-nucleotide-binding protein Gi2.
The Biochemical journal 1990;267(2):391-8.
1990: D Strassheim; Graeme Milligan; Miles D Houslay
Diabetes abolishes the GTP-dependent, but not the receptor-dependent inhibitory function of the inhibitory guanine-nucleotide-binding regulatory protein (Gi) on adipocyte adenylate cyclase activity.
The Biochemical journal 1990;266(2):521-6.
1990: Graeme Milligan; Ian Mullaney; F R McKenzie
Specificity of interactions of receptors and effectors with GTP-binding proteins in native membranes.
Biochemical Society symposium 1990;56():21-34.
1990: Graeme Milligan; F R McKenzie; Steven McClue; F M Mitchell; Ian Mullaney
Guanine nucleotide binding proteins in neuroblastoma x glioma hybrid, NG108-15, cells. Regulation of expression and function.
The International journal of biochemistry 1990;22(7):701-7.
1990: K G Macleod; Graeme Milligan
Biphasic regulation of adenylate cyclase by cholera toxin in neuroblastoma x glioma hybrid cells is due to the activation and subsequent loss of the alpha subunit of the stimulatory GTP binding protein (GS).
Cellular signalling 1990;2(2):139-51.
1990: Graeme Milligan; F M Mitchell; Ian Mullaney; Steven McClue; F R McKenzie
The role and specificity of guanine nucleotide binding proteins in receptor-effector coupling.
Symposia of the Society for Experimental Biology 1990;44():157-72.
1989: Graeme Milligan; Cecilia G Unson; Michael J O Wakelam
Cholera toxin treatment produces down-regulation of the alpha-subunit of the stimulatory guanine-nucleotide-binding protein (Gs).
The Biochemical journal 1989;262(2):643-9.
1989: F M Mitchell; S L Griffiths; E D Saggerson; Miles D Houslay; J T Knowler; Graeme Milligan
Guanine-nucleotide-binding proteins expressed in rat white adipose tissue. Identification of both mRNAs and proteins corresponding to Gi1, Gi2 and Gi3.
The Biochemical journal 1989;262(2):403-8.
1989: J J Ohisalo; Graeme Milligan
Guanine-nucleotide-binding proteins Gi and Gs in fat-cells from normal, hypothyroid and obese human subjects.
The Biochemical journal 1989;260(3):843-7.
1989: Graeme Milligan; Cecilia G Unson
Persistent activation of the alpha subunit of Gs promotes its removal from the plasma membrane.
The Biochemical journal 1989;260(3):837-41.
1989: S D Gardner; Graeme Milligan; J E Rice; Michael J O Wakelam
The effect of cholera toxin on the inhibition of vasopressin-stimulated inositol phospholipid hydrolysis is a cyclic AMP-mediated event at the level of receptor binding.
The Biochemical journal 1989;259(3):679-84.
1989: Graeme Milligan; Shireen A Davies; Miles D Houslay; Michael J O Wakelam
Identification of the pertussis and cholera toxin substrates in normal and N-ras transformed NIH3T3 fibroblasts and an assessment of their involvement in bombesin-stimulation of inositol phospholipid metabolism.
Oncogene 1989;4(5):659-63.
1989: Graeme Milligan
Guanine nucleotide-binding proteins and cellular control.
Current opinion in cell biology 1989;1(2):196-200.
1989: G J Murphy; D J Gawler; Graeme Milligan; Michael J O Wakelam; N J Pyne; Miles D Houslay
Glucagon desensitization of adenylate cyclase and stimulation of inositol phospholipid metabolism does not involve the inhibitory guanine nucleotide regulatory protein Gi, which is inactivated upon challenge of hepatocytes with glucagon.
The Biochemical journal 1989;259(1):191-7.
1989: Graeme Milligan; Z Tanfin; O Goureau; Cecilia G Unson; Simone Harbon
Identification of both Gi2 and a novel, immunologically distinct, form of Go in rat myometrial membranes.
FEBS letters 1989;244(2):411-6.
1989: Ian Mullaney; Graeme Milligan
Elevated levels of the guanine nucleotide binding protein, Go, are associated with differentiation of neuroblastoma x glioma hybrid cells.
FEBS letters 1989;244(1):113-8.
1989: N J Pyne; G J Murphy; Graeme Milligan; Miles D Houslay
Treatment of intact hepatocytes with either the phorbol ester TPA or glucagon elicits the phosphorylation and functional inactivation of the inhibitory guanine nucleotide regulatory protein Gi.
FEBS letters 1989;243(1):77-82.
1989: Miles D Houslay; D J Gawler; Graeme Milligan; A Wilson
Multiple defects occur in the guanine nucleotide regulatory protein system in liver plasma membranes of obese (fa/fa) but not lean (Fa/Fa) Zucker rats: loss of functional Gi and abnormal Gs function.
Cellular signalling 1989;1(1):9-22.
1989: Graeme Milligan; Michael J O Wakelam
G-proteins and second messengers in mitogenesis.
Progress in growth factor research 1989;1(3):161-77.
1989: Graeme Milligan
Foetal calf serum enhances cholera toxin-catalysed ADP-ribosylation of the pertussis toxin-sensitive guanine nucleotide binding protein, Gi2, in rat glioma C6BU1 cells.
Cellular signalling 1989;1(1):65-74.
1989: Graeme Milligan
Tissue distribution and subcellular location of guanine nucleotide binding proteins: implications for cellular signalling.
Cellular signalling 1989;1(5):411-9.
1988: Ian Mullaney; A I Magee; Cecilia G Unson; Graeme Milligan
Differential regulation of amounts of the guanine-nucleotide-binding proteins Gi and Go in neuroblastoma x glioma hybrid cells in response to dibutyryl cyclic AMP.
The Biochemical journal 1988;256(2):649-56.
1988: Graeme Milligan
Techniques used in the identification and analysis of function of pertussis toxin-sensitive guanine nucleotide binding proteins.
The Biochemical journal 1988;255(1):1-13.
1988: Graeme Milligan; Ian Mullaney; Cecilia G Unson; L Marshall; Allen Spiegel; H McArdle
GTP analogues promote release of the alpha subunit of the guanine nucleotide binding protein, Gi2, from membranes of rat glioma C6 BU1 cells.
The Biochemical journal 1988;254(2):391-6.
1988: F R McKenzie; Ian Mullaney; Cecilia G Unson; Allen Spiegel; Graeme Milligan
The use of anti-peptide antisera to probe interactions between receptors and guanine nucleotide binding proteins.
Biochemical Society transactions 1988;16(4):434-7.
1988: Graeme Milligan; F R McKenzie
Opioid peptides promote cholera-toxin-catalysed ADP-ribosylation of the inhibitory guanine-nucleotide-binding protein (Gi) in membranes of neuroblastoma x glioma hybrid cells.
The Biochemical journal 1988;252(2):369-73.
1988: H McArdle; Ian Mullaney; A Magee; Cecilia G Unson; Graeme Milligan
GTP analogues cause release of the alpha subunit of the GTP binding protein, GO, from the plasma membrane of NG108-15 cells.
Biochemical and biophysical research communications 1988;152(1):243-51.
1988: S J Butler; E C Kelly; F R McKenzie; S B Guild; Michael J O Wakelam; Graeme Milligan
Differential effects of suramin on the coupling of receptors to individual species of pertussis-toxin-sensitive guanine-nucleotide-binding proteins.
The Biochemical journal 1988;251(1):201-5.
1988: F R McKenzie; E C Kelly; Cecilia G Unson; Allen Spiegel; Graeme Milligan
Antibodies which recognize the C-terminus of the inhibitory guanine-nucleotide-binding protein (Gi) demonstrate that opioid peptides and foetal-calf serum stimulate the high-affinity GTPase activity of two separate pertussis-toxin substrates.
The Biochemical journal 1988;249(3):653-9.
1988: D Gawler; Graeme Milligan; Miles D Houslay
Treatment of streptozotocin-diabetic rats with metformin restores the ability of insulin to inhibit adenylate cyclase activity and demonstrates that insulin does not exert this action through the inhibitory guanine nucleotide regulatory protein Gi.
The Biochemical journal 1988;249(2):537-42.
1987: N J Pyne; N Anderson; B E Lavan; Graeme Milligan; Hugh G Nimmo; Miles D Houslay
Specific antibodies and the selective inhibitor ICI 118233 demonstrate that the hormonally stimulated 'dense-vesicle' and peripheral-plasma-membrane cyclic AMP phosphodiesterases display distinct tissue distributions in the rat.
The Biochemical journal 1987;248(3):897-901.
1987: Graeme Milligan; Allen Spiegel; Cecilia G Unson; E D Saggerson
Chemically induced hypothyroidism produces elevated amounts of the alpha subunit of the inhibitory guanine nucleotide binding protein (Gi) and the beta subunit common to all G-proteins.
The Biochemical journal 1987;247(1):223-7.
1987: Graeme Milligan
Foetal-calf serum stimulates a pertussis-toxin-sensitive high-affinity GTPase activity in rat glioma C6 BU1 cells.
The Biochemical journal 1987;245(2):501-5.