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Atsushi Miyawaki

This is a preview profile on BiomedExperts - the first literature-based scientific social network. It brings the right researchers together and allows them to collaborate online. Collexis and Dell provide the BiomedExperts network of +1.5 Million pre-calculated profiles free of charge to researchers worldwide.

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Tomura Michio; Mori Yoshiko Saito; Watanabe Ryousuke; Tanaka Masato; Miyawaki Atsushi; Kanagawa Osami
Time-lapse observation of cellular function with fluorescent probe reveals novel CTL-target cell interactions.
Shimozono Satoshi; Tsutsui Hidekazu; Miyawaki Atsushi
Diffusion of large molecules into assembling nuclei revealed using an optical highlighting technique.
Kobiyama Kouji; Takeshita Fumihiko; Ishii Ken J; Koyama Shohei; Aoshi Taiki; Akira Shizuo; Sakaue-Sawano Asako; Miyawaki Atsushi; Yamanaka Yuko; Hirano Hisashi; Suzuki Koichi; Okuda Kenji
A signaling polypeptide derived from an innate immune adaptor molecule can be harnessed as a new class of vaccine adjuvant.

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All Publications

2009: Tomura Michio; Mori Yoshiko Saito; Watanabe Ryousuke; Tanaka Masato; Miyawaki Atsushi; Kanagawa Osami
Time-lapse observation of cellular function with fluorescent probe reveals novel CTL-target cell interactions.
International immunology 2009;21(10):1145-50.
2009: Shimozono Satoshi; Tsutsui Hidekazu; Miyawaki Atsushi
Diffusion of large molecules into assembling nuclei revealed using an optical highlighting technique.
Biophysical journal 2009;97(5):1288-94.
2009: Kobiyama Kouji; Takeshita Fumihiko; Ishii Ken J; Koyama Shohei; Aoshi Taiki; Akira Shizuo; Sakaue-Sawano Asako; Miyawaki Atsushi; Yamanaka Yuko; Hirano Hisashi; Suzuki Koichi; Okuda Kenji
A signaling polypeptide derived from an innate immune adaptor molecule can be harnessed as a new class of vaccine adjuvant.
Journal of immunology (Baltimore, Md. : 1950) 2009;182(3):1593-601.
2008: Asselberghs Inge; Flors Cristina; Ferrighi Lara; Botek Edith; Champagne Benoît; Mizuno Hideaki; Ando Ryoko; Miyawaki Atsushi; Hofkens Johan; Van der Auweraer Mark; Clays Koen
Second-harmonic generation in GFP-like proteins.
Journal of the American Chemical Society 2008;130(46):15713-9.
2008: Fukano Takashi; Shimozono Satoshi; Miyawaki Atsushi
Development of microscopic systems for high-speed dual-excitation ratiometric Ca2+ imaging.
Brain cell biology 2008;36(1-4):43-52.
2008: Tsutsui Hidekazu; Karasawa Satoshi; Okamura Yasushi; Miyawaki Atsushi
Improving membrane voltage measurements using FRET with new fluorescent proteins.
Nature methods 2008;5(8):683-5.
2008: Kogure Takako; Kawano Hiroyuki; Abe Yukiko; Miyawaki Atsushi
Fluorescence imaging using a fluorescent protein with a large Stokes shift.
Methods (San Diego, Calif.) 2008;45(3):223-6.
2008: Sakaue-Sawano Asako; Kurokawa Hiroshi; Morimura Toshifumi; Hanyu Aki; Hama Hiroshi; Osawa Hatsuki; Kashiwagi Saori; Fukami Kiyoko; Miyata Takaki; Miyoshi Hiroyuki; Imamura Takeshi; Ogawa Masaharu; Masai Hisao; Miyawaki Atsushi
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Cell 2008;132(3):487-98.
2008: Takeshita Fumihiko; Kobiyama Kouji; Miyawaki Atsushi; Jounai Nao; Okuda Kenji
The non-canonical role of Atg family members as suppressors of innate antiviral immune signaling.
Autophagy 2008;4(1):67-9.
2008: Katayama Hiroyuki; Yamamoto Akitsugu; Mizushima Noboru; Yoshimori Tamotsu; Miyawaki Atsushi
GFP-like proteins stably accumulate in lysosomes.
Cell structure and function 2008;33(1):1-12.
2008: Shimozono Satoshi; Miyawaki Atsushi
Engineering FRET constructs using CFP and YFP.
Methods in cell biology 2008;85():381-93.
2007: Dedecker Peter; Hotta Jun-ichi; Flors Cristina; Sliwa Michel; Uji-i Hiroshi; Roeffaers Maarten B J; Ando Ryoko; Mizuno Hideaki; Miyawaki Atsushi; Hofkens Johan
Subdiffraction imaging through the selective donut-mode depletion of thermally stable photoswitchable fluorophores: numerical analysis and application to the fluorescent protein Dronpa.
Journal of the American Chemical Society 2007;129(51):16132-41.
2007: Flors Cristina; Hotta Jun-ichi; Uji-i Hiroshi; Dedecker Peter; Ando Ryoko; Mizuno Hideaki; Miyawaki Atsushi; Hofkens Johan
A stroboscopic approach for fast photoactivation-localization microscopy with Dronpa mutants.
Journal of the American Chemical Society 2007;129(45):13970-7.
2007: Miyawaki Atsushi; Karasawa Satoshi
Memorizing spatiotemporal patterns.
Nature chemical biology 2007;3(10):598-601.
2007: Hayashi Ikuko; Mizuno Hideaki; Tong Kit I; Furuta Toshiaki; Tanaka Fujie; Yoshimura Masato; Miyawaki Atsushi; Ikura Mitsuhiko
Crystallographic evidence for water-assisted photo-induced peptide cleavage in the stony coral fluorescent protein Kaede.
Journal of molecular biology 2007;372(4):918-26.
2007: Jounai Nao; Takeshita Fumihiko; Kobiyama Kouji; Sawano Asako; Miyawaki Atsushi; Xin Ke-Qin; Ishii Ken J; Kawai Taro; Akira Shizuo; Suzuki Koichi; Okuda Kenji
The Atg5 Atg12 conjugate associates with innate antiviral immune responses.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(35):14050-5.
2007: Ando Ryoko; Flors Cristina; Mizuno Hideaki; Hofkens Johan; Miyawaki Atsushi
Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants.
Biophysical journal 2007;92(12):L97-9.
2007: Fron Eduard; Flors Cristina; Schweitzer Gerd; Habuchi Satoshi; Mizuno Hideaki; Ando Ryoko; Schryver Frans C De; Miyawaki Atsushi; Hofkens Johan
Ultrafast excited-state dynamics of the photoswitchable protein Dronpa.
Journal of the American Chemical Society 2007;129(16):4870-1.
2007: Fukano Takashi; Sawano Asako; Ohba Yusuke; Matsuda Michiyuki; Miyawaki Atsushi
Differential Ras activation between caveolae/raft and non-raft microdomains.
Cell structure and function 2007;32(1):9-15.
2007: Truong Kevin; Sawano Asako; Miyawaki Atsushi; Ikura Mitsuhiko
Calcium indicators based on calmodulin-fluorescent protein fusions.
Methods in molecular biology (Clifton, N.J.) 2007;352():71-82.
2006: Hara Chikako; Tateyama Kiyohiko; Akamatsu Naoki; Imabayashi Hiroyuki; Karaki Koichi; Nomura Nobuo; Okano Hideyuki; Miyawaki Atsushi
A practical device for pinpoint delivery of molecules into multiple neurons in culture.
Brain cell biology 2006;35(4-6):229-37.
2006: Chan Mitchell C Y; Karasawa Satoshi; Mizuno Hideaki; Bosanac Ivan; Ho Dona; Privé Gilbert G; Miyawaki Atsushi; Ikura Mitsuhiko
Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus.
The Journal of biological chemistry 2006;281(49):37813-9.
2006: Dedecker Peter; Hotta Jun-ichi; Ando Ryoko; Miyawaki Atsushi; Engelborghs Yves; Hofkens Johan
Fast and reversible photoswitching of the fluorescent protein dronpa as evidenced by fluorescence correlation spectroscopy.
Biophysical journal 2006;91(5):L45-7.
2006: Kazuno An-a; Munakata Kae; Nagai Takeharu; Shimozono Satoshi; Tanaka Masashi; Yoneda Makoto; Kato Nobumasa; Miyawaki Atsushi; Kato Tadafumi
Identification of mitochondrial DNA polymorphisms that alter mitochondrial matrix pH and intracellular calcium dynamics.
PLoS genetics 2006;2(8):e128.
2006: Habuchi Satoshi; Dedecker Peter; Hotta Jun-ichi; Flors Cristina; Ando Ryoko; Mizuno Hideaki; Miyawaki Atsushi; Hofkens Johan
Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: mechanism responsible for the reversible photoswitching.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology 2006;5(6):567-76.
2006: Shimozono Satoshi; Hosoi Haruko; Mizuno Hideaki; Fukano Takashi; Tahara Tahei; Miyawaki Atsushi
Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer.
Biochemistry 2006;45(20):6267-71.
2006: Kogure Takako; Karasawa Satoshi; Araki Toshio; Saito Kenta; Kinjo Masataka; Miyawaki Atsushi
A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy.
Nature biotechnology 2006;24(5):577-81.
2006: Fukano Takashi; Shimozono Satoshi; Miyawaki Atsushi
Fast dual-excitation ratiometry with light-emitting diodes and high-speed liquid crystal shutters.
Biochemical and biophysical research communications 2006;340(1):250-5.
2005: Miyawaki Atsushi
Innovations in the imaging of brain functions using fluorescent proteins.
Neuron 2005;48(2):189-99.
2005: Habuchi Satoshi; Ando Ryoko; Dedecker Peter; Verheijen Wendy; Mizuno Hideaki; Miyawaki Atsushi; Hofkens Johan
Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(27):9511-6.
2005: Tsutsui Hidekazu; Karasawa Satoshi; Shimizu Hideaki; Nukina Nobuyuki; Miyawaki Atsushi
Semi-rational engineering of a coral fluorescent protein into an efficient highlighter.
EMBO reports 2005;6(3):233-8.
2005: Miyawaki Atsushi; Nagai Takeharu; Mizuno Hideaki
Engineering fluorescent proteins.
Advances in biochemical engineering/biotechnology 2005;95():1-15.
2004: Ando Ryoko; Mizuno Hideaki; Miyawaki Atsushi
Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting.
Science (New York, N.Y.) 2004;306(5700):1370-3.
2004: Nagai Takeharu; Yamada Shuichi; Tominaga Takashi; Ichikawa Michinori; Miyawaki Atsushi
Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(29):10554-9.
2004: Fukano Takashi; Hama Hiroshi; Miyawaki Atsushi
Similar diffusibility of membrane proteins across the axon-soma and dendrite-soma boundaries revealed by a novel FRAP technique.
Journal of structural biology 2004;147(1):12-8.
2004: Karasawa Satoshi; Araki Toshio; Nagai Takeharu; Mizuno Hideaki; Miyawaki Atsushi
Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer.
The Biochemical journal 2004;381(Pt 1):307-12.
2004: Nagai Takeharu; Miyawaki Atsushi
A high-throughput method for development of FRET-based indicators for proteolysis.
Biochemical and biophysical research communications 2004;319(1):72-7.
2004: Shimozono Satoshi; Fukano Takashi; Kimura Koutarou D; Mori Ikue; Kirino Yutaka; Miyawaki Atsushi
Slow Ca2+ dynamics in pharyngeal muscles in Caenorhabditis elegans during fast pumping.
EMBO reports 2004;5(5):521-6.
2004: Fukano Takashi; Shimozono Satoshi; Miyawaki Atsushi
Simultaneous dual-excitation ratiometry using orthogonal linear polarized lights.
Biochemical and biophysical research communications 2004;317(1):77-83.
2004: Hama Hiroshi; Hara Chikako; Yamaguchi Kazuhiko; Miyawaki Atsushi
PKC signaling mediates global enhancement of excitatory synaptogenesis in neurons triggered by local contact with astrocytes.
Neuron 2004;41(3):405-15.
2003: Miyawaki Atsushi
Fluorescence imaging of physiological activity in complex systems using GFP-based probes.
Current opinion in neurobiology 2003;13(5):591-6.
2003: Miyawaki Atsushi; Nagai Takeharu; Mizuno Hideaki
Mechanisms of protein fluorophore formation and engineering.
Current opinion in chemical biology 2003;7(5):557-62.
2003: Mizuno Hideaki; Mal Tapas Kumar; Tong Kit I; Ando Ryoko; Furuta Toshiaki; Ikura Mitsuhiko; Miyawaki Atsushi
Photo-induced peptide cleavage in the green-to-red conversion of a fluorescent protein.
Molecular cell 2003;12(4):1051-8.
2003: Miyawaki Atsushi; Sawano Asako; Kogure Takako
Lighting up cells: labelling proteins with fluorophores.
Nature cell biology 2003;Suppl():S1-7.
2003: Karasawa Satoshi; Araki Toshio; Yamamoto-Hino Miki; Miyawaki Atsushi
A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling.
The Journal of biological chemistry 2003;278(36):34167-71.
2003: Fukano Takashi; Miyawaki Atsushi
Whole-field fluorescence microscope with digital micromirror device: imaging of biological samples.
Applied optics 2003;42(19):4119-24.
2003: Miyawaki Atsushi
Visualization of the spatial and temporal dynamics of intracellular signaling.
Developmental cell 2003;4(3):295-305.
2003: Miyawaki Atsushi; Mizuno Hideaki; Nagai Takeharu; Sawano Asako
Development of genetically encoded fluorescent indicators for calcium.
Methods in enzymology 2003;360():202-25.
2002: Rekas Agata; Alattia Jean-René; Nagai Takeharu; Miyawaki Atsushi; Ikura Mitsuhiko
Crystal structure of venus, a yellow fluorescent protein with improved maturation and reduced environmental sensitivity.
The Journal of biological chemistry 2002;277(52):50573-8.
2002: Miyawaki Atsushi
Green fluorescent protein-like proteins in reef Anthozoa animals.
Cell structure and function 2002;27(5):343-7.
2002: Ando Ryoko; Hama Hiroshi; Yamamoto-Hino Miki; Mizuno Hideaki; Miyawaki Atsushi
An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(20):12651-6.
2002: Sawano Asako; Takayama Shuichi; Matsuda Michiyuki; Miyawaki Atsushi
Lateral propagation of EGF signaling after local stimulation is dependent on receptor density.
Developmental cell 2002;3(2):245-57.
2002: Shimozono Satoshi; Fukano Takashi; Nagai Takeharu; Kirino Yutaka; Mizuno Hideaki; Miyawaki Atsushi
Confocal imaging of subcellular Ca2+ concentrations using a dual-excitation ratiometric indicator based on green fluorescent protein.
Science's STKE : signal transduction knowledge environment 2002;2002(125):pl4.
2002: Sawano Asako; Hama Hiroshi; Saito Naoaki; Miyawaki Atsushi
Multicolor imaging of Ca(2+) and protein kinase C signals using novel epifluorescence microscopy.
Biophysical journal 2002;82(2):1076-85.
2002: Nagai Takeharu; Ibata Keiji; Park Eun Sun; Kubota Mie; Mikoshiba Katsuhiko; Miyawaki Atsushi
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications.
Nature biotechnology 2002;20(1):87-90.
2001: Truong K; Sawano A; Mizuno H; Hama H; Tong K I; Mal T K; Miyawaki A; Ikura M
FRET-based in vivo Ca2+ imaging by a new calmodulin-GFP fusion molecule.
Nature structural biology 2001;8(12):1069-73.
2001: Nagai T; Sawano A; Park E S; Miyawaki A
Circularly permuted green fluorescent proteins engineered to sense Ca2+.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(6):3197-202.
2001: Mizuno H; Sawano A; Eli P; Hama H; Miyawaki A
Red fluorescent protein from Discosoma as a fusion tag and a partner for fluorescence resonance energy transfer.
Biochemistry 2001;40(8):2502-10.
2000: Sawano A; Miyawaki A
Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesis.
Nucleic acids research 2000;28(16):E78.

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Atsushi Miyawaki

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