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John Mort

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Chemicals & Drugs

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TOP 3
Michaela Durigova; Hideaki Nagase; John S Mort; Peter J Roughley
MMPs are less efficient than ADAMTS5 in cleaving aggrecan core protein.
Susan Wilson; Saadat Hashamiyan; Lorne Clarke; Paul Saftig; John S Mort; Valeria M Dejica; Dieter Brömme
Glycosaminoglycan-mediated loss of cathepsin K collagenolytic activity in MPS I contributes to osteoclast and growth plate abnormalities.
Eunice R Lee; Lisa Lamplugh; Beata Kluczyk; Charles P Leblond; John S Mort
Neoepitopes reveal the features of type II collagen cleavage and the identity of a collagenase involved in the transformation of the epiphyses anlagen in development.

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All Publications

2011: Michaela Durigova; Hideaki Nagase; John S Mort; Peter J Roughley
MMPs are less efficient than ADAMTS5 in cleaving aggrecan core protein.
Matrix biology : journal of the International Society for Matrix Biology 2011;30(2):145-53.
2009: Susan Wilson; Saadat Hashamiyan; Lorne Clarke; Paul Saftig; John S Mort; Valeria M Dejica; Dieter Brömme
Glycosaminoglycan-mediated loss of cathepsin K collagenolytic activity in MPS I contributes to osteoclast and growth plate abnormalities.
The American journal of pathology 2009;175(5):2053-62.
2009: Eunice R Lee; Lisa Lamplugh; Beata Kluczyk; Charles P Leblond; John S Mort
Neoepitopes reveal the features of type II collagen cleavage and the identity of a collagenase involved in the transformation of the epiphyses anlagen in development.
Developmental dynamics : an official publication of the American Association of Anatomists 2009;238(6):1547-63.
2008: R Navab; Claudio Pedraza; Lucia Fallavollita; N Wang; Eric Chevet; P Auguste; Sarah Jenna; Z You; A Bikfalvi; J Hu; R O'Connor; Ann H Erickson; John S Mort; Pnina Brodt
Loss of responsiveness to IGF-I in cells with reduced cathepsin L expression levels.
Oncogene 2008;27(37):4973-85.
2008: M Durigova; Peter J Roughley; John S Mort
Mechanism of proteoglycan aggregate degradation in cartilage stimulated with oncostatin M.
Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society 2008;16(1):98-104.
2007: Anna Ljunggren; Izabela Redzynia; Marcia Alvarez-Fernandez; Magnus Abrahamson; John S Mort; Joanne C Krupa; Mariusz Jaskolski; Grzegorz Bujacz
Crystal structure of the parasite protease inhibitor chagasin in complex with a host target cysteine protease.
Journal of molecular biology 2007;371(1):137-53.
2007: Solmaz Sahebjam; Rama Khokha; John S Mort
Increased collagen and aggrecan degradation with age in the joints of Timp3(-/-) mice.
Arthritis and rheumatism 2007;56(3):905-9.
2007: Joanne C Krupa; David Shaya; Lianli Chi; Robert J Linhardt; Miroslaw Cygler; Stephen G Withers; John S Mort
Quantitative continuous assay for hyaluronan synthase.
Analytical biochemistry 2007;361(2):218-25.
2006: Lee I Melching; William D Fisher; Eunice R Lee; John S Mort; Peter J Roughley
The cleavage of biglycan by aggrecanases.
Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society 2006;14(11):1147-54.
2006: Eunice R Lee; Lisa Lamplugh; Beata Kluczyk; John S Mort; Charles Philippe Leblond
Protease analysis by neoepitope approach reveals the activation of MMP-9 is achieved proteolytically in a test tissue cartilage model involved in bone formation.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2006;54(9):965-80.
2006: Peter J Roughley; Lee I Melching; Terrence F Heathfield; Richard H Pearce; John S Mort
The structure and degradation of aggrecan in human intervertebral disc.
European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society 2006;15 Suppl 3():S326-32.
2006: Gladys Valverde-Franco; Jean-Sébastien Binette; W Li; H Wang; Sen Chai; F Laflamme; Nicolas Tran-Khanh; E Quenneville; Tineke Meijers; A Robin Poole; John S Mort; Michael D Buschmann; Janet E Henderson
Defects in articular cartilage metabolism and early arthritis in fibroblast growth factor receptor 3 deficient mice.
Human molecular genetics 2006;15(11):1783-92.
2005: Peter J Roughley; Lee I Melching; John S Mort
Insect cell conditioned medium contains an endoglycosidase able to liberate chondroitin sulfate chains from aggrecan.
Matrix biology : journal of the International Society for Matrix Biology 2005;24(5):371-5.
2005: Mandana Mahmoodi; Solmaz Sahebjam; David S Smookler; Rama Khokha; John S Mort
Lack of tissue inhibitor of metalloproteinases-3 results in an enhanced inflammatory response in antigen-induced arthritis.
The American journal of pathology 2005;166(6):1733-40.
2005: John S Mort
Histone deacetylases--a new target for suppression of cartilage degradation?
Arthritis research & therapy 2005;7(4):155-6.
2004: John S Mort; Peter J Roughley
Production of antibodies against degradative neoepitopes in aggrecan.
Methods in molecular medicine 2004;100():237-50.
2003: Joanne C Krupa; Ann Marie Butler; John S Mort
Quantitative bead assay for hyaluronidase and heparinase I.
Analytical biochemistry 2003;319(2):280-6.
2003: John S Mort; Carl R Flannery; Joe Makkerh; Joanne C Krupa; Eunice R Lee
Use of anti-neoepitope antibodies for the analysis of degradative events in cartilage and the molecular basis for neoepitope specificity.
Biochemical Society symposium 2003;(70):107-14.
2002: Robert Sztrolovics; Judy Grover; Gabriella Cs-Szabo; Shui-Liang Shi; Yiping Zhang; John S Mort; Peter J Roughley
The characterization of versican and its message in human articular cartilage and intervertebral disc.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2002;20(2):257-66.
2002: Robert Sztrolovics; Anneliese D Recklies; Peter J Roughley; John S Mort
Hyaluronate degradation as an alternative mechanism for proteoglycan release from cartilage during interleukin-1beta-stimulated catabolism.
The Biochemical journal 2002;362(Pt 2):473-9.
2002: Robert Sztrolovics; Robert J White; Peter J Roughley; John S Mort
The mechanism of aggrecan release from cartilage differs with tissue origin and the agent used to stimulate catabolism.
The Biochemical journal 2002;362(Pt 2):465-72.
2002: Joanne C Krupa; Sadiq Hasnain; Dorit Nägler; Robert Ménard; John S Mort
S2' substrate specificity and the role of His110 and His111 in the exopeptidase activity of human cathepsin B.
The Biochemical journal 2002;361(Pt 3):613-9.
2001: MA Davoli; Lisa Lamplugh; A Beauchemin; K Chan; S Mordier; John S Mort; G Murphy; A J Docherty; Charles Philippe Leblond; Eunice R Lee
Enzymes active in the areas undergoing cartilage resorption during the development of the secondary ossification center in the tibiae of rats aged 0-21 days: II. Two proteinases, gelatinase B and collagenase-3, are implicated in the lysis of collagen fibrils.
Developmental dynamics : an official publication of the American Association of Anatomists 2001;222(1):71-88.
2001: Eunice R Lee; Lisa Lamplugh; MA Davoli; A Beauchemin; K Chan; John S Mort; Charles Philippe Leblond
Enzymes active in the areas undergoing cartilage resorption during the development of the secondary ossification center in the tibiae of rats ages 0-21 days: I. Two groups of proteinases cleave the core protein of aggrecan.
Developmental dynamics : an official publication of the American Association of Anatomists 2001;222(1):52-70.
2000: Alona Pavlova; Joanne C Krupa; John S Mort; Magnus Abrahamson; Ingemar Björk
Cystatin inhibition of cathepsin B requires dislocation of the proteinase occluding loop. Demonstration By release of loop anchoring through mutation of his110.
FEBS letters 2000;487(2):156-60.
2000: C J Billington; P Mason; MC Magny; John S Mort
The slow-binding inhibition of cathepsin K by its propeptide.
Biochemical and biophysical research communications 2000;276(3):924-9.
2000: Maryam Taheri; U Saragovi; Abraham Fuks; Joe Makkerh; John S Mort; Clifford P Stanners
Self recognition in the Ig superfamily. Identification of precise subdomains in carcinoembryonic antigen required for intercellular adhesion.
The Journal of biological chemistry 2000;275(35):26935-43.
2000: Joanne C Krupa; John S Mort
Optimization of detergents for the assay of cathepsins B, L, S, and K.
Analytical biochemistry 2000;283(1):99-103.
1999: Christopher B Little; Carl R Flannery; Clare E Hughes; John S Mort; Peter J Roughley; C Dent; Bruce Caterson
Aggrecanase versus matrix metalloproteinases in the catabolism of the interglobular domain of aggrecan in vitro.
The Biochemical journal 1999;344 Pt 1():61-8.
1999: Robert Sztrolovics; Mauro Alini; John S Mort; Peter J Roughley
Age-related changes in fibromodulin and lumican in human intervertebral discs.
Spine 1999;24(17):1765-71.
1999: Robert Sztrolovics; R J White; A Robin Poole; John S Mort; Peter J Roughley
Resistance of small leucine-rich repeat proteoglycans to proteolytic degradation during interleukin-1-stimulated cartilage catabolism.
The Biochemical journal 1999;339 ( Pt 3)():571-7.
1999: O Quraishi; Dorit Nägler; T Fox; J Sivaraman; Miroslaw Cygler; John S Mort; AC Storer
The occluding loop in cathepsin B defines the pH dependence of inhibition by its propeptide.
Biochemistry 1999;38(16):5017-23.
1999: Dorit Nägler; W Tam; AC Storer; Joanne C Krupa; John S Mort; Robert Ménard
Interdependency of sequence and positional specificities for cysteine proteases of the papain family.
Biochemistry 1999;38(15):4868-74.
1998: John S Mort; MC Magny; Eunice R Lee
Cathepsin B: an alternative protease for the generation of an aggrecan 'metalloproteinase' cleavage neoepitope.
The Biochemical journal 1998;335 ( Pt 3)():491-4.
1998: Eunice R Lee; Lisa Lamplugh; Charles Philippe Leblond; S Mordier; MC Magny; John S Mort
Immunolocalization of the cleavage of the aggrecan core protein at the Asn341-Phe342 bond, as an indicator of the location of the metalloproteinases active in the lysis of the rat growth plate.
The Anatomical record 1998;252(1):117-32.
1998: Y Yu; Wim Vranken; N Goudreau; E de Miguel; MC Magny; John S Mort; R Dupras; AC Storer; Feng Ni
An NMR-based identification of peptide fragments mimicking the interactions of the cathepsin B propeptide.
FEBS letters 1998;429(1):9-16.
1998: Robert Ménard; E Carmona; S Takebe; Éric Dufour; C Plouffe; P Mason; John S Mort
Autocatalytic processing of recombinant human procathepsin L. Contribution of both intermolecular and unimolecular events in the processing of procathepsin L in vitro.
The Journal of biological chemistry 1998;273(8):4478-84.
1998: M Nycander; Sergio Estrada; John S Mort; Magnus Abrahamson; Ingemar Björk
Two-step mechanism of inhibition of cathepsin B by cystatin C due to displacement of the proteinase occluding loop.
FEBS letters 1998;422(1):61-4.
1997: Miroslaw Cygler; John S Mort
Proregion structure of members of the papain superfamily. Mode of inhibition of enzymatic activity.
Biochimie 1997;79(11):645-52.
1997: Robert Sztrolovics; Mauro Alini; Peter J Roughley; John S Mort
Aggrecan degradation in human intervertebral disc and articular cartilage.
The Biochemical journal 1997;326 ( Pt 1)():235-41.
1997: John S Mort; David J Buttle
Cathepsin B.
The international journal of biochemistry & cell biology 1997;29(5):715-20.
1997: Y Iwata; John S Mort; H Tateishi; Eunice R Lee
Macrophage cathepsin L, a factor in the erosion of subchondral bone in rheumatoid arthritis.
Arthritis and rheumatism 1997;40(3):499-509.
1997: C Illy; O Quraishi; J Wang; Enrico O Purisima; Thierry Vernet; John S Mort
Role of the occluding loop in cathepsin B activity.
The Journal of biological chemistry 1997;272(2):1197-202.
1996: R Coulombe; Pawel Grochulski; J Sivaraman; Robert Ménard; John S Mort; Miroslaw Cygler
Structure of human procathepsin L reveals the molecular basis of inhibition by the prosegment.
The EMBO journal 1996;15(20):5492-503.
1996: Peter J Roughley; R J White; John S Mort
Presence of pro-forms of decorin and biglycan in human articular cartilage.
The Biochemical journal 1996;318 ( Pt 3)():779-84.
1996: Peter J Roughley; R J White; Gabriella Cs-Szabó; John S Mort
Changes with age in the structure of fibromodulin in human articular cartilage.
Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society 1996;4(3):153-61.
1996: J Sivaraman; R Coloumbe; MC Magny; P Mason; John S Mort; Miroslaw Cygler
Crystallization of rat procathepsin B.
Acta crystallographica. Section D, Biological crystallography 1996;52(Pt 4):874-5.
1996: R Coulombe; Y Li; S Takebe; Robert Ménard; P Mason; John S Mort; Miroslaw Cygler
Crystallization and preliminary X-ray diffraction studies of human procathepsin L.
Proteins 1996;25(3):398-400.
1996: E Carmona; Éric Dufour; C Plouffe; S Takebe; P Mason; John S Mort; Robert Ménard
Potency and selectivity of the cathepsin L propeptide as an inhibitor of cysteine proteases.
Biochemistry 1996;35(25):8149-57.
1996: Miroslaw Cygler; J Sivaraman; Pawel Grochulski; R Coulombe; AC Storer; John S Mort
Structure of rat procathepsin B: model for inhibition of cysteine protease activity by the proregion.
Structure (London, England : 1993) 1996;4(4):405-16.
1996: Z P Lum; B E Hakala; John S Mort; A D Recklies
Modulation of the catabolic effects of interleukin-1 beta on human articular chondrocytes by transforming growth factor-beta.
Journal of cellular physiology 1996;166(2):351-9.
1996: A P Hollander; T F Heathfield; J J Liu; I Pidoux; Peter J Roughley; John S Mort; A Robin Poole
Enhanced denaturation of the alpha (II) chains of type-II collagen in normal adult human intervertebral discs compared with femoral articular cartilage.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 1996;14(1):61-6.
1995: Eunice R Lee; Lisa Lamplugh; N L Shepard; John S Mort
The septoclast, a cathepsin B-rich cell involved in the resorption of growth plate cartilage.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1995;43(5):525-36.
1995: Z Jia; S Hasnain; Tomoko Hirama; Xavier Lee; John S Mort; Rebecca To; C P Huber
Crystal structures of recombinant rat cathepsin B and a cathepsin B-inhibitor complex. Implications for structure-based inhibitor design.
The Journal of biological chemistry 1995;270(10):5527-33.
1995: T Fox; P Mason; AC Storer; John S Mort
Modification of S1 subsite specificity in the cysteine protease cathepsin B.
Protein engineering 1995;8(1):53-7.
1994: Lukas Mach; John S Mort; Josef Glössl
Noncovalent complexes between the lysosomal proteinase cathepsin B and its propeptide account for stable, extracellular, high molecular mass forms of the enzyme.
The Journal of biological chemistry 1994;269(17):13036-40.
1994: Lukas Mach; John S Mort; Josef Glössl
Maturation of human procathepsin B. Proenzyme activation and proteolytic processing of the precursor to the mature proteinase, in vitro, are primarily unimolecular processes.
The Journal of biological chemistry 1994;269(17):13030-5.
1994: P Lindahl; D Ripoll; Magnus Abrahamson; John S Mort; AC Storer
Evidence for the interaction of valine-10 in cystatin C with the S2 subsite of cathepsin B.
Biochemistry 1994;33(14):4384-92.
1994: Ingemar Björk; E Pol; E Raub-Segall; Magnus Abrahamson; A D Rowan; John S Mort
Differential changes in the association and dissociation rate constants for binding of cystatins to target proteinases occurring on N-terminal truncation of the inhibitors indicate that the interaction mechanism varies with different enzymes.
The Biochemical journal 1994;299 ( Pt 1)():219-25.
1993: Peter J Roughley; R J White; MC Magny; J Liu; R H Pearce; John S Mort
Non-proteoglycan forms of biglycan increase with age in human articular cartilage.
The Biochemical journal 1993;295 ( Pt 2)():421-6.
1993: A D Rowan; R Feng; Y Konishi; John S Mort
Demonstration by electrospray mass spectrometry that the peptidyldipeptidase activity of cathepsin B is capable of rat cathepsin B C-terminal processing.
The Biochemical journal 1993;294 ( Pt 3)():923-7.
1993: Lukas Mach; H Schwihla; K Stüwe; A D Rowan; John S Mort; Josef Glössl
Activation of procathepsin B in human hepatoma cells: the conversion into the mature enzyme relies on the action of cathepsin B itself.
The Biochemical journal 1993;293 ( Pt 2)():437-42.
1993: S Hasnain; Tomoko Hirama; C P Huber; P Mason; John S Mort
Characterization of cathepsin B specificity by site-directed mutagenesis. Importance of Glu245 in the S2-P2 specificity for arginine and its role in transition state stabilization.
The Journal of biological chemistry 1993;268(1):235-40.
1992: T Fox; E de Miguel; John S Mort; AC Storer
Potent slow-binding inhibition of cathepsin B by its propeptide.
Biochemistry 1992;31(50):12571-6.
1992: A D Rowan; P Mason; Lukas Mach; John S Mort
Rat procathepsin B. Proteolytic processing to the mature form in vitro.
The Journal of biological chemistry 1992;267(22):15993-9.
1992: A D Rowan; Lukas Mach; John S Mort
Antibodies to rat procathepsin B recognize the active mature enzyme.
Biological chemistry Hoppe-Seyler 1992;373(7):427-32.
1992: S Hasnain; C P Huber; A Muir; A D Rowan; John S Mort
Investigation of structure function relationships in cathepsin B.
Biological chemistry Hoppe-Seyler 1992;373(7):413-8.
1992: Q Nguyen; John S Mort; Peter J Roughley
Preferential mRNA expression of prostromelysin relative to procollagenase and in situ localization in human articular cartilage.
The Journal of clinical investigation 1992;89(4):1189-97.
1992: S Hasnain; Tomoko Hirama; A Tam; John S Mort
Characterization of recombinant rat cathepsin B and nonglycosylated mutants expressed in yeast. New insights into the pH dependence of cathepsin B-catalyzed hydrolyses.
The Journal of biological chemistry 1992;267(7):4713-21.
1991: Q Nguyen; J Liu; Peter J Roughley; John S Mort
Link protein as a monitor in situ of endogenous proteolysis in adult human articular cartilage.
The Biochemical journal 1991;278 ( Pt 1)():143-7.
1991: J Liu; Peter J Roughley; John S Mort
Identification of human intervertebral disc stromelysin and its involvement in matrix degradation.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 1991;9(4):568-75.
1990: Xavier Lee; F R Ahmed; Tomoko Hirama; C P Huber; David R Rose; Rebecca To; S Hasnain; A Tam; John S Mort
Crystallization of recombinant rat cathepsin B.
The Journal of biological chemistry 1990;265(11):5950-1.
1990: Q Nguyen; John S Mort; Peter J Roughley
Cartilage proteoglycan aggregate is degraded more extensively by cathepsin L than by cathepsin B.
The Biochemical journal 1990;266(2):569-73.
1989: I K Campbell; A Guttman; E E Golds; Peter J Roughley; John S Mort
Variation in the effects of mononuclear cell products from different individuals on metalloproteinase secretion from human articular cartilage.
The Journal of rheumatology 1989;16(12):1552-8.
1989: C R Roberts; Peter J Roughley; John S Mort
Degradation of human proteoglycan aggregate induced by hydrogen peroxide. Protein fragmentation, amino acid modification and hyaluronic acid cleavage.
The Biochemical journal 1989;259(3):805-11.
1989: Q Nguyen; G Murphy; Peter J Roughley; John S Mort
Degradation of proteoglycan aggregate by a cartilage metalloproteinase. Evidence for the involvement of stromelysin in the generation of link protein heterogeneity in situ.
The Biochemical journal 1989;259(1):61-7.
1989: R H Pearce; J M Mathieson; John S Mort; Peter J Roughley
Effect of age on the abundance and fragmentation of link protein of the human intervertebral disc.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 1989;7(6):861-7.
1988: John S Mort; A Tam; D F Steiner; S J Chan
Expression of rat and mouse cathepsin B precursors in Escherichia coli.
Biological chemistry Hoppe-Seyler 1988;369 Suppl():163-7.
1987: John S Mort; I K Campbell; A Guttman; E E Golds; Peter J Roughley
The role of mononuclear cell products as modulators of cartilage degradation.
The Journal of rheumatology 1987;14 Spec No():86-7.
1986: I K Campbell; Peter J Roughley; John S Mort
The action of human articular-cartilage metalloproteinase on proteoglycan and link protein. Similarities between products of degradation in situ and in vitro.
The Biochemical journal 1986;237(1):117-22.
1986: I K Campbell; E E Golds; John S Mort; Peter J Roughley
Human articular cartilage secretes characteristic metal dependent proteinases upon stimulation by mononuclear cell factor.
The Journal of rheumatology 1986;13(1):20-7.
1986: I K Campbell; John S Mort; E E Golds; Peter J Roughley
Mononuclear cell factor modulates the concomitant release of proteoglycan and latent proteinase by human articular cartilage in organ culture.
Agents and actions. Supplements 1986;18():43-6.
1986: John S Mort; A D Recklies
Interrelationship of active and latent secreted human cathepsin B precursors.
The Biochemical journal 1986;233(1):57-63.
1985: A D Recklies; John S Mort
Rat mammary gland in culture secretes a stable high molecular weight form of cathepsin L.
Biochemical and biophysical research communications 1985;131(1):402-7.
1985: A D Recklies; John S Mort
Characterization of a cysteine proteinase secreted by mouse mammary gland.
Cancer research 1985;45(5):2302-7.
1985: John S Mort; A D Recklies; A R Poole
Release of cathepsin B precursors from human and murine tumours.
Progress in clinical and biological research 1985;180():243-5.
1984: John S Mort; A D Recklies; A R Poole
Extracellular presence of the lysosomal proteinase cathepsin B in rheumatoid synovium and its activity at neutral pH.
Arthritis and rheumatism 1984;27(5):509-15.
1984: P C Armour; S Levi; E E Golds; A R Poole; John S Mort; Peter J Roughley
Activation of latent collagenase by serum proteinases that interact with immobilized immunoglobulin G.
Rheumatology international 1984;4(4):151-5.
1983: J D Paquin; M van der Rest; P J Marie; John S Mort; I Pidoux; A Robin Poole; Peter J Roughley
Biochemical and morphologic studies of cartilage from the adult human sacroiliac joint.
Arthritis and rheumatism 1983;26(7):887-95.
1983: John S Mort; M S Leduc; A D Recklies
Characterization of a latent cysteine proteinase from ascitic fluid as a high molecular weight form of cathepsin B.
Biochimica et biophysica acta 1983;755(3):369-75.
1982: A D Recklies; A R Poole; John S Mort
A cysteine proteinase secreted from human breast tumours is immunologically related to cathepsin B.
The Biochemical journal 1982;207(3):633-6.
1982: A D Recklies; John S Mort
A radioimmunoassay for total human cathepsin B.
Clinica chimica acta; international journal of clinical chemistry 1982;123(1-2):127-38.
1982: A D Recklies; John S Mort; A R Poole
Secretion of a thiol proteinase from mouse mammary carcinomas and its characterization.
Cancer research 1982;42(3):1026-32.
1981: John S Mort; M Leduc; A D Recklies
A latent thiol proteinase from ascitic fluid of patients with neoplasia.
Biochimica et biophysica acta 1981;662(2):173-80.
1980: John S Mort; A D Recklies; A R Poole
Characterization of a thiol proteinase secreted by malignant human breast tumours.
Biochimica et biophysica acta 1980;614(1):134-43.