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Takafumi Nagatomo

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Radioligand Assay

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Murad Hossain; Masaji Ishiguro; Habib Abul Muntasir; Mamunur Rashid; Takumichi Sugihara; Mohiuddin Ahmed Bhuiyan; Takafumi Nagatomo
Mechanism of inverse agonist action of sarpogrelate at the constitutively active mutant of human 5-HT2A receptor revealed by molecular modeling.
Mohiuddin Ahmed Bhuiyan; Takafumi Nagatomo
Assessment of homologous internalization of constitutively active N111G mutant of AT(1) receptor.
Mohiuddin Ahmed Bhuiyan; Murad Hossain; Masaji Ishiguro; Takashi Nakamura; Takafumi Nagatomo
Engineered mutation of some important amino acids in angiotensin II type 1 (AT1) receptor increases the binding affinity of AT1-receptor antagonists.

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2012: Murad Hossain; Masaji Ishiguro; Habib Abul Muntasir; Mamunur Rashid; Takumichi Sugihara; Mohiuddin Ahmed Bhuiyan; Takafumi Nagatomo
Mechanism of inverse agonist action of sarpogrelate at the constitutively active mutant of human 5-HT2A receptor revealed by molecular modeling.
Biological & pharmaceutical bulletin 2012;35(9):1553-9.
2010: Mohiuddin Ahmed Bhuiyan; Takafumi Nagatomo
Assessment of homologous internalization of constitutively active N111G mutant of AT(1) receptor.
Methods in enzymology 2010;484():165-77.
2010: Mohiuddin Ahmed Bhuiyan; Murad Hossain; Masaji Ishiguro; Takashi Nakamura; Takafumi Nagatomo
Engineered mutation of some important amino acids in angiotensin II type 1 (AT1) receptor increases the binding affinity of AT1-receptor antagonists.
Journal of pharmacological sciences 2010;113(1):57-65.
2010: Mohiuddin Ahmed Bhuiyan; Murad Hossain; Takashi Nakamura; Masanobu Ozaki; Takafumi Nagatomo
Internalization of constitutively active N111G MUTANT of AT1 receptor induced by angiotensin II-receptor antagonists candesartan, losartan, and telmisartan: comparison with valsartan.
Journal of pharmacological sciences 2010;112(4):459-62.
2009: Mohiuddin Ahmed Bhuiyan; Murad Hossain; Shin-Ichiro Miura; Takashi Nakamura; Masanobu Ozaki; Takafumi Nagatomo
Constitutively active mutant N111G of angiotensin II type 1 (AT(1)) receptor induces homologous internalization through mediation of AT(1)-receptor antagonist.
Journal of pharmacological sciences 2009;111(3):227-34.
2009: Murad Hossain; Mamunur Rashid; Mohiuddin Ahmed Bhuiyan; Takashi Nakamura; Masanobu Ozaki; Takafumi Nagatomo
Mutagenesis of important amino acid reveals unconventional homologous internalization of beta(1)-adrenergic receptor.
Life sciences 2009;85(7-8):339-44.
2009: Saida Abdel Regal Aly; Murad Hossain; Mohiuddin Ahmed Bhuiyan; Takashi Nakamura; Takafumi Nagatomo
Assessment of binding affinity to 5-hydroxytryptamine 2A (5-HT2A) receptor and inverse agonist activity of naftidrofuryl: comparison with those of sarpogrelate.
Journal of pharmacological sciences 2009;110(4):445-50.
2009: Mohiuddin Ahmed Bhuiyan; Masaji Ishiguro; Murad Hossain; Takashi Nakamura; Masanobu Ozaki; Shin-Ichiro Miura; Takafumi Nagatomo
Binding sites of valsartan, candesartan and losartan with angiotensin II receptor 1 subtype by molecular modeling.
Life sciences 2009;85(3-4):136-40.
2008: Murad Hossain; Maruf Ahmed; Mohiuddin Ahmed Bhuiyan; Masaji Ishiguro; Takashi Nakamura; Masanobu Ozaki; Takafumi Nagatomo
Mutation of important amino acid residue of Asp104Lys in human beta(1)-adrenergic receptor triggers functional and constitutive inactivation.
Biological & pharmaceutical bulletin 2008;31(8):1517-22.
2008: Maruf Ahmed; Murad Hossain; Mohiuddin Ahmed Bhuiyan; Masaji Ishiguro; Takashi Tanaka; Ikunobu Muramatsu; Takafumi Nagatomo
Mutational analysis of the alpha 1a-adrenergic receptor binding pocket of antagonists by radioligand binding assay.
Biological & pharmaceutical bulletin 2008;31(4):598-601.
2008: Yuko Nagaoka; Maruf Ahmed; Murad Hossain; Mohiuddin Ahmed Bhuiyan; Masaji Ishiguro; Takashi Nakamura; Masatomo Watanabe; Takafumi Nagatomo
Amino acids of the human alpha1d-adrenergic receptor involved in antagonist binding.
Journal of pharmacological sciences 2008;106(1):114-20.
2002: Mamunur Rashid; Mikio Nakazawa; Takafumi Nagatomo
Effects of sarpogrelate, a novel 5-HT2 antagonist, on 5-HT-induced endothelium-dependent relaxations in porcine coronary artery.
Japanese journal of pharmacology 2002;89(4):405-12.
2002: Mamunur Rashid; Masatomo Watanabe; Mikio Nakazawa; Takafumi Nagatomo
AT-1015, a newly synthesized 5-HT2 receptor antagonist, dissociates slowly from the 5-HT2 receptor sites in rabbit cerebral cortex membrane.
The Journal of pharmacy and pharmacology 2002;54(8):1123-8.
2002: Malika Israilova; Fumiko Suzuki; Takashi Tanaka; Takafumi Nagatomo; Takanobu Taniguchi; Ikunobu Muramatsu
Binding and functional affinity of sarpogrelate, its metabolite m-1 and ketanserin for human recombinant alpha-1-adrenoceptor subtypes.
Pharmacology 2002;65(2):69-73.
2002: Yuki Setoguchi; Toshio Ohnuki; Mamunur Rashid; Takashi Nakamura; Kaoru Hattori; Takafumi Nagatomo; Kenichi Watanabe; Akio Mitomi; Mikio Nakazawa
Effects of chronic administration of sarpogrelate on systolic blood pressure of spontaneously hypertensive rats: comparison with quinapril.
Pharmacology 2002;64(2):71-5.
2001: Mamunur Rashid; Masatomo Watanabe; Mikio Nakazawa; Takafumi Nagatomo
Binding affinity of a newly synthesized 5-HT2 antagonist, AT-1015 (N-[2-[4-(5H-Dibenzo[a,d]cyclohepten-5-ylidene)-piperidino]ethyl]-1-formyl-4-piperidinecarboxamide monohydrochloride monohydrate), in the rabbit platelet membrane.
Biological & pharmaceutical bulletin 2001;24(10):1188-90.
2001: Takafumi Nagatomo; Toshio Ohnuki; M Ishiguro; M Ahmed; Takashi Nakamura
Beta-adrenoceptors: three-dimensional structures and binding sites for ligands.
Japanese journal of pharmacology 2001;87(1):7-13.
2001: Takafumi Nagatomo; Yoshiaki Hosohata; Toshio Ohnuki; Takashi Nakamura; Kaoru Hattori; J Suzuki; M Ishiguro
Bopindolol: pharmacological basis and clinical implications.
Cardiovascular drug reviews 2001;19(1):9-24.
2000: H Gong; Takashi Nakamura; Kaoru Hattori; Toshio Ohnuki; Mamunur Rashid; Mikio Nakazawa; Kenichi Watanabe; Takafumi Nagatomo
A novel 5-HT(2) antagonist, sarpogrelate hydrochloride, shows inhibitory effects on both contraction and relaxation mediated by 5-HT receptor subtypes in porcine coronary arteries.
Pharmacology 2000;61(4):263-8.
2000: H Gong; Mamunur Rashid; Takashi Nakamura; Kaoru Hattori; Mikio Nakazawa; Hideaki Kihara; Ryota Yoshimoto; Takafumi Nagatomo
Inhibitory effects of a newly synthesized 5-HT2 receptor antagonist, AT-1015 (N-[2-[4-(5H-dibenzo[a,d]cyclohepten-5-ylidene)piperidino]-ethyl]-1-for myl-4-piperidinecarboxamide monohydrochloride monohydrate), on contraction and relaxation of pig coronary arteries induced by 5-HT and alpha-methylserotonin: comparison with ketanserin.
Biological & pharmaceutical bulletin 2000;23(9):1105-7.
2000: Takashi Nakamura; A Suzuki; Toshio Ohnuki; Kaoru Hattori; Kenichi Watanabe; H Kurose; T Nagao; Takafumi Nagatomo
Assessment of affinities of propranolol and bopindolol to membranes from COS-7 cell transiently transfected with beta-1- and beta-2-adrenoceptors using a radioligand-binding assay method.
Pharmacology 2000;61(1):6-10.
2000: Takafumi Nagatomo; K Koike
Recent advances in structure, binding sites with ligands and pharmacological function of beta-adrenoceptors obtained by molecular biology and molecular modeling.
Life sciences 2000;66(25):2419-26.
1999: Takashi Nakamura; K Maruyama; Toshio Ohnuki; Kaoru Hattori; Kenichi Watanabe; Takafumi Nagatomo
Tamsulosin: assessment of affinityof (3)H-P razosin binding to two alpha-1- adrenoceptor subtypes in the canine aorta.
Pharmacology 1999;59(5):234-8.
1999: Toshio Ohnuki; Takafumi Nagatomo; M Ishiguro
[Modeling study of three-dimensional structure of human beta adrenoceptors and receptor-antagonists interactions].
Nihon yakurigaku zasshi. Folia pharmacologica Japonica 1999;114 Suppl 1():123P-126P.
1999: K Hattori; J Suzuki; Yoshiaki Hosohata; Toshio Ohnuki; T Nakamura; K Watanabe; Ikunobu Muramatsu; Takafumi Nagatomo
Sustaining effects of bopindolol on inhibitory chronotropic actions in guinea pig atria.
Pharmacology 1999;59(4):183-91.
1999: K Hattori; M Ishiguro; Toshio Ohnuki; T Nakamura; Ikunobu Muramatsu; Takafumi Nagatomo
Identification of binding sites of bopindolol and its two metabolites with beta1-adrenoceptors by molecular modeling: comparison with beta2 adrenoceptors.
Pharmacology 1999;58(4):183-9.
1999: Takashi Nakamura; Yoshiaki Hosohata; Keiko Hosohata; Kaoru Hattori; J Suzuki; Takafumi Nagatomo
Relationship between hydrophobicity and beta-blocking potencies, affinities or dissociation of beta-blockers from beta-adrenoceptors.
Research communications in molecular pathology and pharmacology 1999;106(3):203-11.
1998: K Maruyama; Takashi Nakamura; T Yoshihara; J Fukutomi; K Sugiyama; K Hattorim; Toshio Ohnuki; Kenichi Watanabe; Takafumi Nagatomo
Tamsulosin: assessment of affinity of 3H-prazosin bindings to two alpha1-adrenoceptor subtypes (alpha1H and alpha1L) in bovine prostate and rat heart and brain.
General pharmacology 1998;31(4):597-600.
1998: Yoshiaki Hosohata; Kaoru Hattori; Y Shen; M Okuyama; H Kaneko; Toshio Ohnuki; J Suzuki; Takafumi Nagatomo
Bopindolol: a slowly dissociating antagonist from the beta-adrenoceptors in guinea pig atria.
Pharmacology 1998;57(4):180-7.
1998: K Maruyama; M Suzuki; M Tsuchiya; Y Makara; Kaoru Hattori; Toshio Ohnuki; Kenichi Watanabe; Takafumi Nagatomo
Discrimination of alpha1-adrenoceptor subtypes in rat aorta and prostate.
Pharmacology 1998;57(2):88-95.
1998: Y L Qu; K Sugiyama; T Ohnuki; Kaoru Hattori; Kenichi Watanabe; Takafumi Nagatomo
Comparison of binding affinities of omega-conotoxin and amlodipine to N-type Ca2+ channels in rat brain.
Zhongguo yao li xue bao = Acta pharmacologica Sinica 1998;19(2):97-100.
1998: Takafumi Nagatomo; M Ishiguro; Toshio Ohnuki; Kaoru Hattori; Yoshiaki Hosohata; N Takatsu; H Katayama; Kenichi Watanabe
Studies on relationships between chemical structure and beta-blocking potency of bopindolol and its two metabolites.
Life sciences 1998;62(17-18):1597-600.
1997: T Hiroi; K Maruyama; Kaoru Hattori; Toshio Ohnuki; Takafumi Nagatomo; K Watanabe; T Yamamoto; Y Hara
Affinity for [3H]iloprost binding sites and cAMP synthesis activity of a 3-oxa-methano prostaglandin I1 analog, SM-10906, in human platelets and endothelial cells.
Japanese journal of pharmacology 1997;74(1):37-43.
1997: S Kubo; A Matsuda; Toshio Ohnuki; Kaoru Hattori; J Suzuki; Takafumi Nagatomo
Assessment of beta 2- and beta 3-adrenoceptors in rat white adipose tissues by radioligand binding assay.
Biological & pharmaceutical bulletin 1997;20(2):142-8.
1997: Takafumi Nagatomo; Yoshiaki Hosohata; Toshio Ohnuki; J Suzuki
[Pharmacological characteristics of the long-acting beta-blocker "bopindolol"].
Nihon yakurigaku zasshi. Folia pharmacologica Japonica 1997;109(1):1-12.
1996: K Maruyama; J Fukutomi; T Chiba; M Yamaguchi; M Takeda; Kaoru Hattori; Takafumi Nagatomo
Two district alpha(1)-adrenoceptor subtypes in the human prostate: assessment by radioligand binding assay using 3H-prazosin.
General pharmacology 1996;27(8):1377-81.
1996: Yoshiaki Hosohata; K Sasaki; K Maruyama; Toshio Ohnuki; Kaoru Hattori; J Suzuki; Kenichi Watanabe; Takafumi Nagatomo
Effects of chronic administration of bopindolol on the binding characteristics of cardiac alpha 1H-, alpha 1L-, beta 1- and beta 2-adrenoceptor subtypes in cardiac muscles of spontaneously hypertensive rats (SHR).
Biological & pharmaceutical bulletin 1996;19(7):932-5.
1996: Kenichi Watanabe; Y Ochiai; T Washizuka; T Inomata; Y Miyakita; M Shiba; T Izumi; A Shibata; Y L Qu; Takafumi Nagatomo
Clinical evaluation of serum amlodipine level in patients with angina pectoris.
General pharmacology 1996;27(2):205-9.
1996: K Sugiyama; Y L Qu; K Maruyama; Kaoru Hattori; Kenichi Watanabe; Takafumi Nagatomo
Slow dissociation of long-acting Ca2+ antagonist amlodipine from 3H-PN200-110 binding sites in membranes of rat hearts and brains.
Biological & pharmaceutical bulletin 1996;19(2):195-8.
1996: Y L Qu; K Sugiyama; Kaoru Hattori; A Yamamoto; Kenichi Watanabe; Takafumi Nagatomo
Slow association of positively charged Ca2+ channel antagonist amlodipine to dihydropyridine receptor sites in rat brain membranes.
General pharmacology 1996;27(1):137-40.
1995: Yoshiaki Hosohata; K Sasaki; Y Shen; Kaoru Hattori; J Suzuki; Takafumi Nagatomo
Bopindolol is a slowly dissociating beta 1-adrenoceptor antagonist when compared to other beta-blockers.
Biological & pharmaceutical bulletin 1995;18(8):1066-71.
1995: Y L Qu; C Takamizawa; K Sugiyama; K Maruyama; Kaoru Hattori; Kenichi Watanabe; Takafumi Nagatomo
Residual inhibition in density of [3H]isradipine binding sites in rat brain membrane pretreated with amlodipine.
Zhongguo yao li xue bao = Acta pharmacologica Sinica 1995;16(4):289-93.
1995: Yoshiaki Hosohata; K Sasaki; M Suzuki; Y Karakisawa; K Maruyama; H Tsuchihashi; Takafumi Nagatomo
Alpha-1 and beta-adrenergic receptor blocking potencies of bopindolol and its two metabolites (18-502 and 20-785) as assessed by radioligand binding assay methods.
General pharmacology 1995;26(4):743-7.
1994: Yoshiaki Hosohata; M Suzuki; Y Karakisawa; K Maruyama; Takafumi Nagatomo
The affinity of bopindolol and its two metabolites for a beta 2-adrenoceptor in the bovine mesenteric artery.
Biological & pharmaceutical bulletin 1994;17(9):1296-8.
1994: K Maruyama; N Ohkura; Y Yagi; Takafumi Nagatomo
Comparison of displacemental potencies of terazosin enantiomers for alpha 1-adrenoceptor subtypes.
Biological & pharmaceutical bulletin 1994;17(8):1126-9.
1993: Y L Qu; K Sugiyama; Takafumi Nagatomo; T Maniwa; A Miyagishi
Calcium channel blocking properties of SM-6586 in rat heart and brain as assessed by radioligand binding assay.
Japanese journal of pharmacology 1993;63(2):165-9.
1993: K Maruyama; N Ohkura; Y Yagi; Takafumi Nagatomo
Alpha-1 adrenoceptor subtypes in canine aorta.
Japanese journal of pharmacology 1993;62(3):263-7.
1993: K Watanabe; A Shibata; H Wakabayashi; K Shimada; K Sasaki; Takafumi Nagatomo
Cardiomyopathic hamster hearts: long-term effects of drugs on catecholamine contents and binding characteristics of alpha 1- and beta 1-adrenergic receptors.
Biological & pharmaceutical bulletin 1993;16(7):660-3.
1993: E Satoh; A Narimatsu; Yoshiaki Hosohata; H Tsuchihashi; Takafumi Nagatomo
The affinity of betaxolol, a beta 1-adrenoceptor-selective blocking agent, for beta-adrenoceptors in the bovine trachea and heart.
British journal of pharmacology 1993;108(2):484-9.
1993: J Kinami; H Tsuchihashi; K Maruyama; K Sasaki; Takafumi Nagatomo
Nipradilol displays a unique pharmacological profile of affinities for the different alpha 1-adrenoceptor subtypes.
Japanese journal of pharmacology 1993;61(2):81-6.
1992: H Tsuchihashi; T Sasaki; S Kojima; Takafumi Nagatomo
Binding of [3H]haloperidol to dopamine D2 receptors in the rat striatum.
The Journal of pharmacy and pharmacology 1992;44(11):911-4.
1992: K Maruyama; H Tsuchihashi; S Baba; F Mano; Takafumi Nagatomo
Alpha 1-adrenoceptor subtypes in bovine prostate.
The Journal of pharmacy and pharmacology 1992;44(9):727-30.
1992: J Kinami; H Tsuchihashi; S Baba; F Mano; K Maruyama; Takafumi Nagatomo
Alpha 1-adrenoceptor subtypes in the rat ventricular muscle.
The Journal of pharmacy and pharmacology 1992;44(2):97-100.
1992: J Kinami; Y L Qu; H Tsuchihashi; Takafumi Nagatomo; T Maniwa; A Miyagishi
Assessment of Ca(2+)-antagonistic effect of SM-6586 and its isomers, novel 1,4-dihydropyridine derivatives, by radioligand binding assay.
Japanese journal of pharmacology 1992;58(1):75-8.
1992: K Maruyama; N Ohkura; Y Yagi; H Tshuchihashi; Takafumi Nagatomo
Alpha-1 adrenoceptor subtypes in canine aorta.
Japanese journal of pharmacology 1992;58 Suppl 2():272P.
1991: H Tsuchihashi; K Maruyama; S Baba; F Mano; J Kinami; Takafumi Nagatomo
Comparison of alpha 1-adrenoceptors between rat brain and spleen.
Japanese journal of pharmacology 1991;56(4):523-30.
1991: H Tsuchihashi; N Yagi; M Kimura; K Shirota; J Kinami; Takafumi Nagatomo
Binding characteristics of [3H]ketanserin for serotonin-2 receptor in the rabbit platelet.
Journal of pharmacobio-dynamics 1991;14(8):461-6.
1991: K Maruyama; J Kinami; H Tsuchihashi; Takafumi Nagatomo
Binding characteristics of alpha 1-adrenoceptors in bovine prostate using a radioligand binding assay.
Journal of pharmacobio-dynamics 1991;14(6):315-9.
1991: N Sakuma; H Tsuchihashi; Yoshiaki Hosohata; H Akashi; J Kinami; Takafumi Nagatomo
Beta-blocking potency and selectivity of bopindolol and its two metabolites for beta 1- and beta 2-adrenergic receptors as assessed by radioligand binding assay.
Journal of pharmacobio-dynamics 1991;14(5):250-5.
1991: K Maruyama; J Kinami; Y Sugita; Y Takada; E Sugiyama; H Tsuchihashi; Takafumi Nagatomo
MCI-9042: high affinity for serotonergic receptors as assessed by radioligand binding assay.
Journal of pharmacobio-dynamics 1991;14(4):177-81.
1991: K Watanabe; A Shibata; H Wakabayashi; K Shimada; H Tsuchihashi; J Kinami; Takafumi Nagatomo
Changes in alpha 1- and beta 1-adrenergic receptors and calcium ion binding sites in the fetal myocardium of spontaneously hypertensive rats (SHR).
Journal of pharmacobio-dynamics 1991;14(4):182-6.
1990: H Tsuchihashi; Y Nakashima; J Kinami; Takafumi Nagatomo
Characteristics of 125I-iodocyanopindolol binding to beta-adrenergic and serotonin-1B receptors of rat brain: selectivity of beta-adrenergic agents.
Japanese journal of pharmacology 1990;52(2):195-200.
1989: H Tsuchihashi; Takafumi Nagatomo; S Imai
Three binding sites of 125I-iodocyanopindolol, i.e. beta 1, beta 2-adrenergic and 5HT1B-serotonergic receptors in rat brain determined by the displacement and Scatchard analysis.
Journal of pharmacobio-dynamics 1989;12(9):509-16.
1989: H Tsuchihashi; Takafumi Nagatomo
Alpha-blocking potencies of antihypertensive agents (prazosin, terazosin, bunazosin, SGB-1534 and ketanserin) having with quinazoline or quinazolinedione as assessed by radioligand binding assay methods in rat brain.
Journal of pharmacobio-dynamics 1989;12(3):170-4.
1989: H Tsuchihashi; H Yokoyama; Takafumi Nagatomo
Binding characteristics of 3H-CGP12177 to beta-adrenoceptors in rat myocardial membranes.
Japanese journal of pharmacology 1989;49(1):11-9.
1988: Takafumi Nagatomo; R Hokibara; Y Tanaka; T Nakamura; J Aono; H Tsuchihashi
Effects of ketanserin and 3-[2-[4-(o-methoxyphenyl)-1-piperazinyl]ethyl]-2,4(1H,3H)- quinazolinedione monohydrochloride (SGB-1534), anti-hypertensive agents, on 3H-serotonin and 3H-ketanserin bindings to serotonergic (5HT1 and 5HT2) receptors in dog brain and aorta.
Chemical & pharmaceutical bulletin 1988;36(8):3113-8.
1988: Takafumi Nagatomo; J Aono; A Tajiri; T Nakamura; H Tsuchihashi
Effect of 3-[2-[4-(o-methoxyphenyl)-1-piperazinyl]ethyl]-2,4(1H,3H)- quinazolinedione monophydrochloride (SGB-1534), an antihypertensive agent, on 3H-prazosin and 3H-p-aminoclonidine binding to alpha 1- and alpha 2-adrenoceptors in dog brain and aorta.
Chemical & pharmaceutical bulletin 1988;36(3):1122-7.
1987: H Tsuchihashi; J Aono; Takafumi Nagatomo; T Kawada; H Ohta; S Imai
Effects of bunitrolol on adrenergic and serotonergic receptors.
Japanese journal of pharmacology 1987;45(3):349-56.
1987: Takafumi Nagatomo; A Tajiri; T Nakamura; R Hokibara; Y Tanaka; J Aono; H Tsuchihashi
Adrenergic and serotonergic receptor-blocking potencies of terazosin, a new antihypertensive agent, as assessed by radioligand binding assay.
Chemical & pharmaceutical bulletin 1987;35(4):1629-32.