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Shigeru Nakamori

Research Profile

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Yoshimitsu Hamano; T Yoshida; M Kito; Shigeru Nakamori; T Nagasawa; Hiroshi Takagi
Biological function of the pld gene product that degrades epsilon-poly-L-lysine in Streptomyces albulus.
Satoshi Yamada; Naoki Awano; Kyoko Inubushi; Eri Maeda; Shigeru Nakamori; Kunihiko Nishino; Akihito Yamaguchi; Hiroshi Takagi
Effect of drug transporter genes on cysteine export and overproduction in Escherichia coli.
Yutaka Haitani; Naoki Awano; Mizue Yamazaki; Masaru Wada; Shigeru Nakamori; Hiroshi Takagi
Functional analysis of L-serine O-acetyltransferase from Corynebacterium glutamicum.

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2006: Yoshimitsu Hamano; T Yoshida; M Kito; Shigeru Nakamori; T Nagasawa; Hiroshi Takagi
Biological function of the pld gene product that degrades epsilon-poly-L-lysine in Streptomyces albulus.
Applied microbiology and biotechnology 2006;72(1):173-81.
2006: Satoshi Yamada; Naoki Awano; Kyoko Inubushi; Eri Maeda; Shigeru Nakamori; Kunihiko Nishino; Akihito Yamaguchi; Hiroshi Takagi
Effect of drug transporter genes on cysteine export and overproduction in Escherichia coli.
Applied and environmental microbiology 2006;72(7):4735-42.
2006: Yutaka Haitani; Naoki Awano; Mizue Yamazaki; Masaru Wada; Shigeru Nakamori; Hiroshi Takagi
Functional analysis of L-serine O-acetyltransferase from Corynebacterium glutamicum.
FEMS microbiology letters 2006;255(1):156-63.
2005: Masakazu Takahashi; Kazuhisa Tsujimoto; Youichi Kato; Hideyuki Yamada; Hiroshi Takagi; Shigeru Nakamori
A sericin-derived peptide protects sf9 insect cells from death caused by acute serum deprivation.
Biotechnology letters 2005;27(13):893-7.
2005: Naoki Awano; Masaru Wada; Hirotada Mori; Shigeru Nakamori; Hiroshi Takagi
Identification and functional analysis of Escherichia coli cysteine desulfhydrases.
Applied and environmental microbiology 2005;71(7):4149-52.
2005: Yoshimitsu Hamano; Ine Nicchu; Yusuke Hoshino; Takahiro Kawai; Shigeru Nakamori; Hiroshi Takagi
Development of gene delivery systems for the epsilon-poly-L-lysine producer, Streptomyces albulus.
Journal of bioscience and bioengineering 2005;99(6):636-41.
2004: Masakazu Takahashi; Tomoko Sekine; Naoko Kuba; Shigeru Nakamori; Masaaki Yasuda; Hiroshi Takagi
The production of recombinant APRP, an alkaline protease derived from Bacillus pumilus TYO-67, by in vitro refolding of pro-enzyme fixed on a solid surface.
Journal of biochemistry 2004;136(4):549-56.
2004: Yoshimitsu Hamano; Yusuke Hoshino; Shigeru Nakamori; Hiroshi Takagi
Overexpression and characterization of an aminoglycoside 6'-N-acetyltransferase with broad specificity from an epsilon-poly-L-lysine producer, Streptomyces albulus IFO14147.
Journal of biochemistry 2004;136(4):517-24.
2004: Masaru Wada; Naoki Awano; Hiroyuki Yamazawa; Hiroshi Takagi; Shigeru Nakamori
Purification and characterization of O-Acetylserine sulfhydrylase of Corynebacterium glutamicum.
Bioscience, biotechnology, and biochemistry 2004;68(7):1581-3.
2004: Masaru Wada; Ayumi Yoshizumi; Yuki Furukawa; Hiroshi Kawabata; Makoto Ueda; Hiroshi Takagi; Shigeru Nakamori
Cloning and overexpression of the Exiguobacterium sp. F42 gene encoding a new short chain dehydrogenase, which catalyzes the stereoselective reduction of ethyl 3-oxo-3-(2-thienyl)propanoate to ethyl (S)-3-hydroxy-3-(2-thienyl)propanoate.
Bioscience, biotechnology, and biochemistry 2004;68(7):1481-8.
2003: Yukiyasu Terao; Shigeru Nakamori; Hiroshi Takagi
Gene dosage effect of L-proline biosynthetic enzymes on L-proline accumulation and freeze tolerance in Saccharomyces cerevisiae.
Applied and environmental microbiology 2003;69(11):6527-32.
2003: Chikara Hoshikawa; Mika Shichiri; Shigeru Nakamori; Hiroshi Takagi
A nonconserved Ala401 in the yeast Rsp5 ubiquitin ligase is involved in degradation of Gap1 permease and stress-induced abnormal proteins.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(20):11505-10.
2003: Yusuke Hoshino; Shigeru Nakamori; Hiroshi Takagi
Cloning and analysis of the beta-lactamase gene from epsilon-poly-L-lysine-producing actinomycete Streptomyces albulus IFO14147.
Journal of biochemistry 2003;134(3):473-8.
2003: Masaru Wada; Shigeru Nakamori; Hiroshi Takagi
Serine racemase homologue of Saccharomyces cerevisiae has L-threo-3-hydroxyaspartate dehydratase activity.
FEMS microbiology letters 2003;225(2):189-93.
2003: Naoki Awano; Masaru Wada; A Kohdoh; T Oikawa; Hiroshi Takagi; Shigeru Nakamori
Effect of cysteine desulfhydrase gene disruption on L-cysteine overproduction in Escherichia coli.
Applied microbiology and biotechnology 2003;62(2-3):239-43.
2003: Masaru Wada; Ayumi Yoshizumi; Yumiko Noda; Michihiko Kataoka; Sakayu Shimizu; Hiroshi Takagi; Shigeru Nakamori
Production of a doubly chiral compound, (4R,6R)-4-hydroxy-2,2,6-trimethylcyclohexanone, by two-step enzymatic asymmetric reduction.
Applied and environmental microbiology 2003;69(2):933-7.
2003: Hiroshi Takagi; Kenji Yoshioka; Naoki Awano; Shigeru Nakamori; Bun-ichiro Ono
Role of Saccharomyces cerevisiae serine O-acetyltransferase in cysteine biosynthesis.
FEMS microbiology letters 2003;218(2):291-7.
2003: Michiyo Nomura; Shigeru Nakamori; Hiroshi Takagi
Characterization of novel acetyltransferases found in budding and fission yeasts that detoxify a proline analogue, azetidine-2-carboxylic acid.
Journal of biochemistry 2003;133(1):67-74.
2003: Yuko Morita; Shigeru Nakamori; Hiroshi Takagi
L-proline accumulation and freeze tolerance of Saccharomyces cerevisiae are caused by a mutation in the PRO1 gene encoding gamma-glutamyl kinase.
Applied and environmental microbiology 2003;69(1):212-9.
2002: Yasuko Kimura; Shigeru Nakamori; Hiroshi Takagi
Polymorphism of the MPR1 gene required for toxic proline analogue resistance in the Saccharomyces cerevisiae complex species.
Yeast (Chichester, England) 2002;19(16):1437-45.
2002: Masaru Wada; Naoki Awano; Keiko Haisa; Hiroshi Takagi; Shigeru Nakamori
Purification, characterization and identification of cysteine desulfhydrase of Corynebacterium glutamicum, and its relationship to cysteine production.
FEMS microbiology letters 2002;217(1):103-7.
2002: Yuko Morita; Shigeru Nakamori; Hiroshi Takagi
Effect of proline and arginine metabolism on freezing stress of Saccharomyces cerevisiae.
Journal of bioscience and bioengineering 2002;94(5):390-4.
2001: Hiroshi Takagi; M Koga; S Katsurada; Yukihiro Yabuta; Ujwal Shinde; Masayori Inouye; Shigeru Nakamori
Functional analysis of the propeptides of subtilisin E and aqualysin I as intramolecular chaperones.
FEBS letters 2001;508(2):210-4.
2001: M Shichiri; C Hoshikawa; Shigeru Nakamori; Hiroshi Takagi
A novel acetyltransferase found in Saccharomyces cerevisiae Sigma1278b that detoxifies a proline analogue, azetidine-2-carboxylic acid.
The Journal of biological chemistry 2001;276(45):41998-2002.
2001: Masakazu Takahashi; Y Hasuura; Shigeru Nakamori; Hiroshi Takagi
Improved autoprocessing efficiency of mutant subtilisins E with altered specificity by engineering of the pro-region.
Journal of biochemistry 2001;130(1):99-106.
2001: A Yoshisumi; Masaru Wada; Hiroshi Takagi; Sakayu Shimizu; Shigeru Nakamori
Cloning, sequence analysis, and expression in Escherichia coli of the gene encoding monovalent cation-activated levodione reductase from Corynebacterium aquaticum M-13.
Bioscience, biotechnology, and biochemistry 2001;65(4):830-6.
2000: Hiroshi Takagi; K Hirai; M Wada; Shigeru Nakamori
Enhanced thermostability of the single-Cys mutant subtilisin E under oxidizing conditions.
Journal of biochemistry 2000;128(4):585-9.
2000: Hiroshi Takagi; M Shichiri; M Takemura; M Mohri; Shigeru Nakamori
Saccharomyces cerevisiae sigma 1278b has novel genes of the N-acetyltransferase gene superfamily required for L-proline analogue resistance.
Journal of bacteriology 2000;182(15):4249-56.
2000: Hiroshi Takagi; A Suzumura; Y Hasuura; T Hoshino; Shigeru Nakamori
Efficient selection for thermostable protease in Thermus thermophilus.
Bioscience, biotechnology, and biochemistry 2000;64(4):899-902.
2000: Hiroshi Takagi; K Hirai; Y Maeda; H Matsuzawa; Shigeru Nakamori
Engineering subtilisin E for enhanced stability and activity in polar organic solvents.
Journal of biochemistry 2000;127(4):617-25.
2000: Hiroshi Takagi; K Sakai; K Morida; Shigeru Nakamori
Proline accumulation by mutation or disruption of the proline oxidase gene improves resistance to freezing and desiccation stresses in Saccharomyces cerevisiae.
FEMS microbiology letters 2000;184(1):103-8.
2000: Hiroshi Takagi; Yusuke Hoshino; Shigeru Nakamori; S Inouye
Isolation and sequence analysis of plasmid pNO33 in the epsilon-poly-L-lysine-producing actinomycete Streptomyces albulus IFO14147.
Journal of bioscience and bioengineering 2000;89(1):94-6.
1999: Hiroshi Takagi; Naoki Awano; SI Kobayashi; M Noji; Kazuki Saito; Shigeru Nakamori
Overproduction of L-cysteine and L-cystine by expression of genes for feedback inhibition-insensitive serine acetyltransferase from Arabidopsis thaliana in Escherichia coli.
FEMS microbiology letters 1999;179(2):453-9.
1999: Masaru Wada; Ayumi Yoshizumi; Shigeru Nakamori; Sakayu Shimizu
Purification and characterization of monovalent cation-activated levodione reductase from Corynebacterium aquaticum M-13.
Applied and environmental microbiology 1999;65(10):4399-403.
1999: Masaru Wada; T Matsumoto; Shigeru Nakamori; M Sakamoto; Michihiko Kataoka; J Q Liu; N Itoh; H Yamada; Sakayu Shimizu
Purification and characterization of a novel enzyme, L-threo-3-hydroxyaspartate dehydratase, from Pseudomonas sp. T62.
FEMS microbiology letters 1999;179(1):147-51.
1999: Shigeru Nakamori; S Kobayashi; T Nishimura; Hiroshi Takagi
Mechanism of L-methionine overproduction by Escherichia coli: the replacement of Ser-54 by Asn in the MetJ protein causes the derepression of L-methionine biosynthetic enzymes.
Applied microbiology and biotechnology 1999;52(2):179-85.
1999: Hiroshi Takagi; C Kobayashi; SI Kobayashi; Shigeru Nakamori
PCR random mutagenesis into Escherichia coli serine acetyltransferase: isolation of the mutant enzymes that cause overproduction of L-cysteine and L-cystine due to the desensitization to feedback inhibition.
FEBS letters 1999;452(3):323-7.
1999: Masaru Wada; Hiroshi Kawabata; Ayumi Yoshizumi; Michihiko Kataoka; Shigeru Nakamori; Yoshihiko Yasohara; Noriyuki Kizaki; J Hasegawa; Sakayu Shimizu
Occurrence of multiple ethyl 4-chloro-3-oxobutanoate-reducing enzymes in Candida magnoliae.
Journal of bioscience and bioengineering 1999;87(2):144-8.
1998: Hiroshi Takagi; M Yamamoto; I Ohtsu; Shigeru Nakamori
Random mutagenesis into the conserved Gly154 of subtilisin E: isolation and characterization of the revertant enzymes.
Protein engineering 1998;11(12):1205-10.
1998: Shigeru Nakamori; SI Kobayashi; C Kobayashi; Hiroshi Takagi
Overproduction of L-cysteine and L-cystine by Escherichia coli strains with a genetically altered serine acetyltransferase.
Applied and environmental microbiology 1998;64(5):1607-11.
1997: Hiroshi Takagi; I Ohtsu; Shigeru Nakamori
Construction of novel subtilisin E with high specificity, activity and productivity through multiple amino acid substitutions.
Protein engineering 1997;10(9):985-9.
1997: Hiroshi Takagi; F Iwamoto; Shigeru Nakamori
Isolation of freeze-tolerant laboratory strains of Saccharomyces cerevisiae from proline-analogue-resistant mutants.
Applied microbiology and biotechnology 1997;47(4):405-11.
1996: Hiroshi Takagi; T Maeda; I Ohtsu; Y C Tsai; Shigeru Nakamori
Restriction of substrate specificity of subtilisin E by introduction of a side chain into a conserved glycine residue.
FEBS letters 1996;395(2-3):127-32.